Restriction map for pF1KB7794
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AvrII       BbeI        
BbrPI       BcgI        BciVI       BfrI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrDI       BsrGI       Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoRI       EcoRV       EcoT22I     FseI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MaeII       MamI        MfeI        MluI        
MseI        MunI        NarI        NdeI        NheI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SbfI        ScaI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        TfiI        Tru9I       TthHB8I     XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI         1240  GTCTAC               GT^[AC][GT]AC
AccIII        881  TCCGGA               T^CCGGA
AfaI         1440  GTAC                 GT^AC
ApaI         1530  GGGCCC               GGGCC^C
Asp700I       570  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII          99  TGCGCA               TGC^GCA
BalI          458  TGGCCA               TGG^CCA
BamHI          46  GGATCC               G^GATCC
BanI         1477  GGCACC               G^G[CT][GA]CC
BbsI         1216  GAAGAC               GAAGAC(2/6)
BfaI          952  CTAG                 C^TAG
BpuAI        1216  GAAGAC               GAAGAC(2/6)
BseAI         881  TCCGGA               T^CCGGA
BsgI          604  GTGCAG               GTGCAG(16/14)
BsmI         1020  GAATGC               GAATGC(1/-1)
BspEI         881  TCCGGA               T^CCGGA
BsrFI        1371  GCCGGC               [GA]^CCGG[CT]
BssHII        703  GCGCGC               G^CGCGC
BstEII       1068  GGTCACC              G^GT[ACGT]ACC
BstPI        1068  GGTCACC              G^GT[ACGT]ACC
Cfr10I       1371  GCCGGC               [GA]^CCGG[CT]
EcoO65I      1068  GGTCACC              G^GT[ACGT]ACC
FspI           99  TGCGCA               TGC^GCA
KspI          446  CCGCGG               CCGC^GG
MaeI          952  CTAG                 C^TAG
MluNI         458  TGGCCA               TGG^CCA
MroI          881  TCCGGA               T^CCGGA
MscI          458  TGGCCA               TGG^CCA
NaeI         1371  GCCGGC               GCC^GGC
NgoMIV       1371  GCCGGC               G^CCGGC
NotI          448  GCGGCCGC             GC^GGCCGC
PshAI        1062  GACTCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI         1440  GTAC                 GT^AC
SacII         446  CCGCGG               CCGC^GG
SapI          198  GAAGAGC              GCTCTTC(1/4)
SexAI         599  ACCTGGT              A^CC[AT]GGT
SfiI          450  GGCCGCCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp509I       755  AATT                 ^AATT
XbaI          951  TCTAGA               T^CTAGA
XcmI          464  CCACCCCGCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          570  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII         447  CGCG                 CG^CG
              704  CGCG                
AlwNI        1042  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1060  CAGACTCTG           
AspI            4  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1234  GACGGCGTC           
BbvCI         480  CCTCAGC              CCTCAGC(-5/-2)
              814  GCTGAGG             
BclI          545  TGATCA               T^GATCA
             1407  TGATCA              
BglI          427  GCCCCCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              451  GCCGCCATGGC         
BglII         902  AGATCT               A^GATCT
             1079  AGATCT              
BsaI         1009  GAGACC               GGTCTC(1/5)
             1429  GAGACC              
BsaWI         591  ACCGGA               [AT]^CCGG[AT]
              881  TCCGGA              
BsiEI          26  CGGCCG               CG[GA][CT]^CG
              449  CGGCCG              
BspMI        1120  ACCTGC               ACCTGC(4/8)
             1397  ACCTGC              
BsrBI         666  CCGCTC               CCGCTC(-3/-3)
              270  GAGCGG              
BssSI        1426  CACGAG               CACGAG(-5/-1)
             1168  CTCGTG              
BstUI         447  CGCG                 CG^CG
              704  CGCG                
EagI           26  CGGCCG               C^GGCCG
              449  CGGCCG              
EarI          198  GAAGAG               CTCTTC(1/4)
              873  GAAGAG              
EclXI          26  CGGCCG               C^GGCCG
              449  CGGCCG              
Eco52I         26  CGGCCG               C^GGCCG
              449  CGGCCG              
FauI          468  CCCGC                CCCGC(4/6)
              665  CCCGC               
FbaI          545  TGATCA               T^GATCA
             1407  TGATCA              
HaeII         282  AGCGCC               [GA]GCGC^[CT]
              351  GGCGCT              
Ksp632I       198  GAAGAG               CTCTTC(1/4)
              873  GAAGAG              
MvnI          447  CGCG                 CG^CG
              704  CGCG                
PflFI           4  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1234  GACGGCGTC           
PflMI        1059  CCAGACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1502  CCAAGGAGTGG         
PstI         1048  CTGCAG               CTGCA^G
             1399  CTGCAG              
PvuII         535  CAGCTG               CAG^CTG
             1045  CAGCTG              
SfcI         1048  CTGCAG               C^T[GA][CT]AG
             1399  CTGCAG              
StuI          866  AGGCCT               AGG^CCT
             1451  AGGCCT              
Tth111I         4  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1234  GACGGCGTC           
Van91I       1059  CCAGACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1502  CCAAGGAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AspHI       
AvaI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoNI       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       Sau3AI      
Sau96I      ScrFI       SfaNI       SmaI        StyI        
TseI        Tsp45I      TspRI       XhoII       XmaCI       
XmaI        
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