Restriction map for pF1KB7794
 (Linear) (Six-base) MAPSORT of: KIBB7794.seq  Check: 1187  from: 1  to: 1557



KIBB7794.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1406    1557 
   Size:       1406     151
 
AccI GT'mk_AC

Cuts at:      0    1241    1557 
   Size:       1241     316
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1158    1339    1342    1557 
   Size:       1158     181       3     215

  Fragments arranged by size: 

                1158     215     181       3
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1047    1065    1557 
   Size:       1047      18     492

  Fragments arranged by size: 

                1047     492      18
 
ApaI G_GGCC'C

Cuts at:      0    1534    1557 
   Size:       1534      23
 
AvaI C'yCGr_G

Cuts at:      0     519    1183    1363    1388    1557 
   Size:        519     664     180      25     169

  Fragments arranged by size: 

                 664     519     180     169      25
 
BamHI G'GATC_C

Cuts at:      0      46    1557 
   Size:         46    1511
 
BanI G'GyrC_C

Cuts at:      0    1477    1557 
   Size:       1477      80
 
BanII G_rGCy'C

Cuts at:      0     205     295     400    1165    1340    1534    1557 
   Size:        205      90     105     765     175     194      23

  Fragments arranged by size: 

                 765     205     194     175     105      90      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1223    1557 
   Size:       1223     334
 
BbvCI CC'TCA_GC

Cuts at:      0     481     815    1557 
   Size:        481     334     742

  Fragments arranged by size: 

                 742     481     334
 
BclI T'GATC_A

Cuts at:      0     545    1407    1557 
   Size:        545     862     150

  Fragments arranged by size: 

                 862     545     150
 
BglI GCCn_nnn'nGGC

Cuts at:      0     433     457    1557 
   Size:        433      24    1100

  Fragments arranged by size: 

                1100     433      24
 
BglII A'GATC_T

Cuts at:      0     902    1079    1557 
   Size:        902     177     478

  Fragments arranged by size: 

                 902     478     177
 
Bme1580I G_kGCm'C

Cuts at:      0     305    1175    1534    1557 
   Size:        305     870     359      23

  Fragments arranged by size: 

                 870     359     305      23
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1124    1557 
   Size:       1124     433
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     165    1209    1557 
   Size:        165    1044     348

  Fragments arranged by size: 

                1044     348     165
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583     666    1024    1036    1042    1068    1557 
   Size:        583      83     358      12       6      26     489

  Fragments arranged by size: 

                 583     489     358      83      26      12       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     110     268     481     815    1557 
   Size:        110     158     213     334     742

  Fragments arranged by size: 

                 742     334     213     158     110
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1003    1423    1557 
   Size:       1003     420     134

  Fragments arranged by size: 

                1003     420     134
 
BsaHI Gr'CG_yC

Cuts at:      0       5     619    1238    1557 
   Size:          5     614     619     319

  Fragments arranged by size: 

                 619     614     319       5
 
BsaWI w'CCGG_w

Cuts at:      0     591     881    1557 
   Size:        591     290     676

  Fragments arranged by size: 

                 676     591     290
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     247     277    1066    1096    1557 
   Size:        247      30     789      30     461

  Fragments arranged by size: 

                 789     461     247      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     699     727     805     808     874    1182    1557 
   Size:        699      28      78       3      66     308     375

  Fragments arranged by size: 

                 699     375     308      78      66      28       3
 
BseYI C'CCAG_C

Cuts at:      0     354     474     625    1158    1349    1557 
   Size:        354     120     151     533     191     208

  Fragments arranged by size: 

                 533     354     208     191     151     120
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625    1557 
   Size:        625     932
 
BsiEI CG_ry'CG

Cuts at:      0      29     452    1557 
   Size:         29     423    1105

  Fragments arranged by size: 

                1105     423      29
 
BsiHKAI G_wGCw'C

Cuts at:      0     116     257     373    1557 
   Size:        116     141     116    1184

  Fragments arranged by size: 

                1184     141     116     116
 
BsmI GAATG_Cn'

Cuts at:      0    1026    1557 
   Size:       1026     531
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     205     257     295     305     373     400    1165 
   Size:        116      89      52      38      10      68      27     765

Cuts at:   1165    1175    1340    1534    1557 
   Size:         10     165     194      23

  Fragments arranged by size: 

                 765     194     165     116      89      68      52      38
                  27      23      10      10
 
BspEI T'CCGG_A

Cuts at:      0     881    1557 
   Size:        881     676
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1129    1406    1557 
   Size:       1129     277     151

  Fragments arranged by size: 

                1129     277     151
 
BsrBI CCG'CTC

Cuts at:      0     272     668    1557 
   Size:        272     396     889

  Fragments arranged by size: 

                 889     396     272
 
BsrFI r'CCGG_y

Cuts at:      0    1371    1557 
   Size:       1371     186
 
BssHII G'CGCG_C

Cuts at:      0     703    1557 
   Size:        703     854
 
BssSI C'ACGA_G

Cuts at:      0    1168    1426    1557 
   Size:       1168     258     131

  Fragments arranged by size: 

                1168     258     131
 
BstEII G'GTnAC_C

Cuts at:      0    1068    1557 
   Size:       1068     489
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      57     312    1349    1557 
   Size:         57     255    1037     208

  Fragments arranged by size: 

                1037     255     208      57
 
BstYI r'GATC_y

Cuts at:      0      46      69     902    1079    1524    1557 
   Size:         46      23     833     177     445      33

  Fragments arranged by size: 

                 833     445     177      46      33      23
 
BtgI C'CryG_G

Cuts at:      0     410     437     446     455     633     698     713    1331 
   Size:        410      27       9       9     178      65      15     618

Cuts at:   1331    1557 
   Size:        226

  Fragments arranged by size: 

                 618     410     226     178      65      27      15       9
                   9
 
BtsI GCAGTG_nn'

Cuts at:      0     645     740     775    1557 
   Size:        645      95      35     782

  Fragments arranged by size: 

                 782     645      95      35
 
EaeI y'GGCC_r

Cuts at:      0      26     434     449     458     716    1100    1226    1247 
   Size:         26     408      15       9     258     384     126      21

Cuts at:   1247    1311    1557 
   Size:         64     246

  Fragments arranged by size: 

                 408     384     258     246     126      64      26      21
                  15       9
 
EagI C'GGCC_G

Cuts at:      0      26     449    1557 
   Size:         26     423    1108

  Fragments arranged by size: 

                1108     423      26
 
EarI CTCTTCn'nnn_

Cuts at:      0     193     868    1557 
   Size:        193     675     689

  Fragments arranged by size: 

                 689     675     193
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     265    1557 
   Size:        265    1292
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583     666    1024    1036    1042    1068    1557 
   Size:        583      83     358      12       6      26     489

  Fragments arranged by size: 

                 583     489     358      83      26      12       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     236     492     787    1447    1557 
   Size:        236     256     295     660     110

  Fragments arranged by size: 

                 660     295     256     236     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0     516     597    1031    1118    1531    1557 
   Size:        516      81     434      87     413      26

  Fragments arranged by size: 

                 516     434     413      87      81      26
 
FspI TGC'GCA

Cuts at:      0     101    1557 
   Size:        101    1456
 
FspAI rTGC'GCAy

Cuts at:      0     101    1557 
   Size:        101    1456
 
HaeII r_GCGC'y

Cuts at:      0     286     355    1557 
   Size:        286      69    1202

  Fragments arranged by size: 

                1202     286      69
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     640    1557 
   Size:         62     578     917

  Fragments arranged by size: 

                 917     578      62
 
MscI TGG'CCA

Cuts at:      0     460    1557 
   Size:        460    1097
 
MslI CAynn'nnrTG

Cuts at:      0     310     379     638    1557 
   Size:        310      69     259     919

  Fragments arranged by size: 

                 919     310     259      69
 
MspA1I CmG'CkG

Cuts at:      0     448     471     537    1047    1383    1557 
   Size:        448      23      66     510     336     174

  Fragments arranged by size: 

                 510     448     336     174      66      23
 
NaeI GCC'GGC

Cuts at:      0    1373    1557 
   Size:       1373     184
 
NcoI C'CATG_G

Cuts at:      0     437     455     633     698    1331    1557 
   Size:        437      18     178      65     633     226

  Fragments arranged by size: 

                 633     437     226     178      65      18
 
NgoMIV G'CCGG_C

Cuts at:      0    1371    1557 
   Size:       1371     186
 
NotI GC'GGCC_GC

Cuts at:      0     449    1557 
   Size:        449    1108
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1065    1508    1557 
   Size:       1065     443      49

  Fragments arranged by size: 

                1065     443      49
 
PfoI T'CCnGG_A

Cuts at:      0      56    1557 
   Size:         56    1501
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     889     921    1557 
   Size:        889      32     636

  Fragments arranged by size: 

                 889     636      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     597    1031    1118    1557 
   Size:        597     434      87     439

  Fragments arranged by size: 

                 597     439     434      87
 
PshAI GACnn'nnGTC

Cuts at:      0    1066    1557 
   Size:       1066     491
 
PspOMI G'GGCC_C

Cuts at:      0    1530    1557 
   Size:       1530      27
 
PstI C_TGCA'G

Cuts at:      0    1052    1403    1557 
   Size:       1052     351     154

  Fragments arranged by size: 

                1052     351     154
 
PvuII CAG'CTG

Cuts at:      0     537    1047    1557 
   Size:        537     510     510

  Fragments arranged by size: 

                 537     510     510
 
SacII CC_GC'GG

Cuts at:      0     449    1557 
   Size:        449    1108
 
SapI GCTCTTCn'nnn_

Cuts at:      0     193    1557 
   Size:        193    1364
 
SexAI A'CCwGG_T

Cuts at:      0     599    1557 
   Size:        599     958
 
SfcI C'TryA_G

Cuts at:      0    1048    1399    1557 
   Size:       1048     351     158

  Fragments arranged by size: 

                1048     351     158
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     457    1557 
   Size:        457    1100
 
SmaI CCC'GGG

Cuts at:      0     521    1365    1390    1557 
   Size:        521     844      25     167

  Fragments arranged by size: 

                 844     521     167      25
 
StuI AGG'CCT

Cuts at:      0     868    1453    1557 
   Size:        868     585     104

  Fragments arranged by size: 

                 868     585     104
 
StyI C'CwwG_G

Cuts at:      0     437     455     633     698    1331    1502    1557 
   Size:        437      18     178      65     633     171      55

  Fragments arranged by size: 

                 633     437     178     171      65      55      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     340    1123    1438    1557 
   Size:        340     783     315     119

  Fragments arranged by size: 

                 783     340     315     119
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     247     279    1557 
   Size:        247      32    1278

  Fragments arranged by size: 

                1278     247      32
 
Tth111I GACn'n_nGTC

Cuts at:      0       7    1237    1557 
   Size:          7    1230     320

  Fragments arranged by size: 

                1230     320       7
 
XbaI T'CTAG_A

Cuts at:      0     951    1557 
   Size:        951     606
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     471    1557 
   Size:        471    1086
 
XmaI C'CCGG_G

Cuts at:      0     519    1363    1388    1557 
   Size:        519     844      25     169

  Fragments arranged by size: 

                 844     519     169      25
 
XmnI GAAnn'nnTTC

Cuts at:      0     574    1557 
   Size:        574     983
 

 Enzymes that do cut:

     AarI     AccI     AlfI    AlwNI     ApaI     AvaI    BamHI     BanI
    BanII     BbsI    BbvCI     BclI     BglI    BglII Bme1580I     BmrI
     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI   BssHII    BssSI   BstEII    BstXI    BstYI     BtgI
     BtsI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I
     FspI    FspAI    HaeII     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NotI    PflMI     PfoI     PpiI    PpuMI    PshAI
   PspOMI     PstI    PvuII    SacII     SapI    SexAI     SfcI     SfiI
     SmaI     StuI     StyI    TaqII     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI     BcgI    BciVI    BfrBI     BlpI    BmgBI
     BmtI    BpuEI    BsaAI    BsaBI    BsiWI    BsmBI    BspHI    BsrDI
    BsrGI   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI  EcoICRI    EcoRI    EcoRV     FalI     FseI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NdeI     NheI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI    SanDI     SbfI     ScaI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     XhoI     ZraI
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