Restriction map for pF1KB6708
 (Circular) (Six-base) MAPSORT of: pF1KB6708.seq  Check: 1082  from: 1  to: 3500



pF1KB6708  3500 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:    538     538 
   Size:       3500
 
Acc65I G'GTAC_C

Cuts at:    516     516 
   Size:       3500
 
AclI AA'CG_TT

Cuts at:   3340    3340 
   Size:       3500
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    302    1325    1757    2504    2772     302 
   Size:       1023     432     747     268    1030

  Fragments arranged by size: 

                1030    1023     747     432     268
 
AflIII A'CryG_T

Cuts at:    492     852    1956    2933     492 
   Size:        360    1104     977    1059

  Fragments arranged by size: 

                1104    1059     977     360
 
AgeI A'CCGG_T

Cuts at:   1888    1888 
   Size:       3500
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    200     232     200 
   Size:         32    3468
 
AlwNI CAG_nnn'CTG

Cuts at:    156     486     805    2372     156 
   Size:        330     319    1567    1284

  Fragments arranged by size: 

                1567    1284     330     319
 
ApaLI G'TGCA_C

Cuts at:   2270    2270 
   Size:       3500
 
ApoI r'AATT_y

Cuts at:    504    2853    3109     504 
   Size:       2349     256     895

  Fragments arranged by size: 

                2349     895     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3500
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3500
 
AvaI C'yCGr_G

Cuts at:    520     520 
   Size:       3500
 
BamHI G'GATC_C

Cuts at:    525     525 
   Size:       3500
 
BanI G'GyrC_C

Cuts at:    322     474     516     930    1178    1213     322 
   Size:        152      42     414     248      35    2609

  Fragments arranged by size: 

                2609     414     248     152      42      35
 
BanII G_rGCy'C

Cuts at:    165     462     514    1544     165 
   Size:        297      52    1030    2121

  Fragments arranged by size: 

                2121    1030     297      52
 
BbeI G_GCGC'C

Cuts at:    934    1182     934 
   Size:        248    3252
 
BbsI GAAGACnn'nnnn_

Cuts at:    338     338 
   Size:       3500
 
BciVI GTATCCnnnnn_n'

Cuts at:   1398    2159    3500    1398 
   Size:        761    1341    1398

  Fragments arranged by size: 

                1398    1341     761
 
BclI T'GATC_A

Cuts at:   1020    1020 
   Size:       3500
 
BglI GCCn_nnn'nGGC

Cuts at:    151     160     176     151 
   Size:          9      16    3475

  Fragments arranged by size: 

                3475      16       9
 
BglII A'GATC_T

Cuts at:   1015    3052    1015 
   Size:       2037    1463
 
BlpI GC'TnA_GC

Cuts at:    612     612 
   Size:       3500
 
Bme1580I G_kGCm'C

Cuts at:    479    1125    1218    2274     479 
   Size:        646      93    1056    1705

  Fragments arranged by size: 

                1705    1056     646      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    167     251     712     793    1128    3272     167 
   Size:         84     461      81     335    2144     395

  Fragments arranged by size: 

                2144     461     395     335      84      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1867    1867 
   Size:       3500
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    227     227 
   Size:       3500
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    676    1555    2047    2345     676 
   Size:        879     492     298    1831

  Fragments arranged by size: 

                1831     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3204    3204 
   Size:       3500
 
BsaAI yAC'GTr

Cuts at:    853     853 
   Size:       3500
 
BsaBI GATnn'nnATC

Cuts at:      6    1014    3051       6 
   Size:       1008    2037     455

  Fragments arranged by size: 

                2037    1008     455
 
BsaHI Gr'CG_yC

Cuts at:    931    1179    1881    3377     931 
   Size:        248     702    1496    1054

  Fragments arranged by size: 

                1496    1054     702     248
 
BsaWI w'CCGG_w

Cuts at:    689    1210    1888    2162    2309    2720     689 
   Size:        521     678     274     147     411    1469

  Fragments arranged by size: 

                1469     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    200     230     200 
   Size:         30    3470
 
BseYI C'CCAG_C

Cuts at:    437     479     925    1683    2260    2756     437 
   Size:         42     446     758     577     496    1181

  Fragments arranged by size: 

                1181     758     577     496     446      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    406     406 
   Size:       3500
 
BsiEI CG_ry'CG

Cuts at:    104     185    1042    1088    2296     104 
   Size:         81     857      46    1208    1308

  Fragments arranged by size: 

                1308    1208     857      81      46
 
BsiHKAI G_wGCw'C

Cuts at:    514    1292    1482    2274     514 
   Size:        778     190     792    1740

  Fragments arranged by size: 

                1740     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    165     462     479     514    1125    1218    1292    1482    1544 
   Size:        297      17      35     611      93      74     190      62

Cuts at:   1544    2274     165 
   Size:        730    1391

  Fragments arranged by size: 

                1391     730     611     297     190      93      74      62
                  35      17
 
BspEI T'CCGG_A

Cuts at:   2720    2720 
   Size:       3500
 
BspHI T'CATG_A

Cuts at:   2676    2676 
   Size:       3500
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    550     764    1066    1447     550 
   Size:        214     302     381    2603

  Fragments arranged by size: 

                2603     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1792    1846    3330    3495      50 
   Size:       1742      54    1484     165      55

  Fragments arranged by size: 

                1742    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1412    1412 
   Size:       3500
 
BsrFI r'CCGG_y

Cuts at:   1498    1679    1888    1498 
   Size:        181     209    3110

  Fragments arranged by size: 

                3110     209     181
 
BssSI C'ACGA_G

Cuts at:   1771    2129    1771 
   Size:        358    3142
 
BstAPI GCAn_nnn'nTGC

Cuts at:    877     877 
   Size:       3500
 
BstBI TT'CG_AA

Cuts at:   1861    1861 
   Size:       3500
 
BstYI r'GATC_y

Cuts at:      1     525     992    1015    1350    1596    2608    2694    2706 
   Size:        524     467      23     335     246    1012      86      12

Cuts at:   2706    3052       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     524     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    157     157 
   Size:       3500
 
BtgI C'CryG_G

Cuts at:    107     197     107 
   Size:         90    3410
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    202     456    1450    1631    1794     202 
   Size:        254     994     181     163    1908

  Fragments arranged by size: 

                1908     994     254     181     163
 
BtsI GCAGTG_nn'

Cuts at:    743     743 
   Size:       3500
 
DraI TTT'AAA

Cuts at:    499     499 
   Size:       3500
 
DrdI GACnn_nn'nnGTC

Cuts at:   1206    2064    1206 
   Size:        858    2642
 
EaeI y'GGCC_r

Cuts at:    143     182    1085    1259    1650    1677    3022     143 
   Size:         39     903     174     391      27    1345     621

  Fragments arranged by size: 

                1345     903     621     391     174      39      27
 
EagI C'GGCC_G

Cuts at:    182    1085     182 
   Size:        903    2597
 
EarI CTCTTCn'nnn_

Cuts at:    224     383    1523    1733     224 
   Size:        159    1140     210    1991

  Fragments arranged by size: 

                1991    1140     210     159
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1708    2018    2164    3310    1708 
   Size:        310     146    1146    1898

  Fragments arranged by size: 

                1898    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    512     512 
   Size:       3500
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    227     302    1325    1757    2504    2772     227 
   Size:         75    1023     432     747     268     955

  Fragments arranged by size: 

                1023     955     747     432     268      75
 
EcoO109I rG'GnC_Cy

Cuts at:    153     639     153 
   Size:        486    3014
 
EcoRI G'AATT_C

Cuts at:    504     504 
   Size:       3500
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1519    1519 
   Size:       3500
 
FspI TGC'GCA

Cuts at:   1281    1281 
   Size:       3500
 
HaeII r_GCGC'y

Cuts at:    934    1182    2204    3183     934 
   Size:        248    1022     979    1251

  Fragments arranged by size: 

                1251    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1342    1372    1374    1404    1342 
   Size:         30       2      30    3438

  Fragments arranged by size: 

                3438      30      30       2
 
HincII GTy'rAC

Cuts at:    539     539 
   Size:       3500
 
KasI G'GCGC_C

Cuts at:    930    1178     930 
   Size:        248    3252
 
KpnI G_GTAC'C

Cuts at:    520     520 
   Size:       3500
 
MluI A'CGCG_T

Cuts at:   2933    2933 
   Size:       3500
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    227     383    2171    2355     227 
   Size:        156    1788     184    1372

  Fragments arranged by size: 

                1788    1372     184     156
 
MscI TGG'CCA

Cuts at:   1261    1261 
   Size:       3500
 
MslI CAynn'nnrTG

Cuts at:   1489    1616    1489 
   Size:        127    3373
 
MspA1I CmG'CkG

Cuts at:    452     486     611     925    1285    2298    2543     452 
   Size:         34     125     314     360    1013     245    1409

  Fragments arranged by size: 

                1409    1013     360     314     245     125      34
 
NaeI GCC'GGC

Cuts at:   1681    1681 
   Size:       3500
 
NarI GG'CG_CC

Cuts at:    931    1179     931 
   Size:        248    3252
 
NcoI C'CATG_G

Cuts at:    107     197     107 
   Size:         90    3410
 
NgoMIV G'CCGG_C

Cuts at:   1679    1679 
   Size:       3500
 
NotI GC'GGCC_GC

Cuts at:    182     182 
   Size:       3500
 
NspI r_CATG'y

Cuts at:    553    1053    1960     553 
   Size:        500     907    2093

  Fragments arranged by size: 

                2093     907     500
 
PciI A'CATG_T

Cuts at:   1956    1956 
   Size:       3500
 
PflMI CCAn_nnn'nTGG

Cuts at:    486     486 
   Size:       3500
 
PmeI GTTT'AAAC

Cuts at:    499     499 
   Size:       3500
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    200     232    2639    2671     200 
   Size:         32    2407      32    1029

  Fragments arranged by size: 

                2407    1029      32      32
 
PstI C_TGCA'G

Cuts at:    547    1232     547 
   Size:        685    2815
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3500
 
PvuII CAG'CTG

Cuts at:    486     925    1285     486 
   Size:        439     360    2701

  Fragments arranged by size: 

                2701     439     360
 
RsrII CG'GwC_CG

Cuts at:   1695    1695 
   Size:       3500
 
SacI G_AGCT'C

Cuts at:    514     514 
   Size:       3500
 
SalI G'TCGA_C

Cuts at:    537     537 
   Size:       3500
 
SapI GCTCTTCn'nnn_

Cuts at:    224    1523    1733     224 
   Size:       1299     210    1991

  Fragments arranged by size: 

                1991    1299     210
 
SbfI CC_TGCA'GG

Cuts at:    547     547 
   Size:       3500
 
ScaI AGT'ACT

Cuts at:   3017    3017 
   Size:       3500
 
SfcI C'TryA_G

Cuts at:     33     543    1228    2221    2412      33 
   Size:        510     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     510     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    151     151 
   Size:       3500
 
SfoI GGC'GCC

Cuts at:    932    1180     932 
   Size:        248    3252
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3500
 
SmaI CCC'GGG

Cuts at:    522     522 
   Size:       3500
 
SmlI C'TyrA_G

Cuts at:    655    1570    2062    2324     655 
   Size:        915     492     262    1831

  Fragments arranged by size: 

                1831     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3500
 
SphI G_CATG'C

Cuts at:    553    1053     553 
   Size:        500    3000
 
StyI C'CwwG_G

Cuts at:    107     197     634     988     107 
   Size:         90     437     354    2619

  Fragments arranged by size: 

                2619     437     354      90
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1083    1216    1675    1884    1083 
   Size:        133     459     209    2699

  Fragments arranged by size: 

                2699     459     209     133
 
TatI w'GTAC_w

Cuts at:    297    3015     297 
   Size:       2718     782
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3017    3049    3017 
   Size:         32    3468
 
Tth111I GACn'n_nGTC

Cuts at:   1297    1297 
   Size:       3500
 
XbaI T'CTAG_A

Cuts at:    531     531 
   Size:       3500
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    995     995 
   Size:       3500
 
XmaI C'CCGG_G

Cuts at:    520     520 
   Size:       3500
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI
     NspI     PciI    PflMI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
   Bpu10I    BseRI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NruI     NsiI
     PacI     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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