Restriction map for pF1KA0034
##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AatII       AcyI        AgeI        AhdI        Aor51HI     
ApaI        AscI        AspEI       AviII       AvrII       
BbeI        BcgI        BglI        BglII       BlnI        
BsaHI       BsiEI       BsiWI       BsmBI       BspLU11I    
BsrBI       BssHII      Bst1107I    BstAPI      BstZ17I     
CpoI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      FauI        FseI        FspI        Hin1I       
HindIII     KasI        KspI        MfeI        MluI        
MunI        NaeI        NarI        NcoI        NgoMIV      
NotI        NruI        PacI        PinAI       PmeI        
PshAI       PvuI        RsrII       SacII       SbfI        
SfiI        SfoI        SgrAI       SmaI        Sse8387I    
SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   73 enzymes) #
###################################

Acc65I        868  GGTACC               G^GTACC
AccI         2429  GTCGAC               GT^[AC][GT]AC
AccIII       3215  TCCGGA               T^CCGGA
AflII        2287  CTTAAG               C^TTAAG
Alw44I       2179  GTGCAC               G^TGCAC
AocI         1437  CCTAAGG              CC^T[ACGT]AGG
ApaLI        2179  GTGCAC               G^TGCAC
AseI         2791  ATTAAT               AT^TAAT
AsnI         2791  ATTAAT               AT^TAAT
Asp718I       868  GGTACC               G^GTACC
AspI         2344  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         3491  CTCGAG               C^[CT]CG[GA]G
BalI         2769  TGGCCA               TGG^CCA
BbrPI        3113  CACGTG               CAC^GTG
BbvCI        2539  GCTGAGG              CCTCAGC(-5/-2)
BclI         3896  TGATCA               T^GATCA
BfrI         2287  CTTAAG               C^TTAAG
BlpI         4486  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     4486  GCTCAGC              GC^T[ACGT]AGC
BsaBI        2737  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3215  TCCGGA               [AT]^CCGG[AT]
BseAI        3215  TCCGGA               T^CCGGA
BsmI         1690  GCATTC               GAATGC(1/-1)
BsoBI        3491  CTCGAG               C^[CT]CG[GA]G
BspDI        2336  ATCGAT               AT^CGAT
BspEI        3215  TCCGGA               T^CCGGA
BsrFI        1072  GCCGGT               [GA]^CCGG[CT]
BssSI        4298  CTCGTG               CACGAG(-5/-1)
BstBI         185  TTCGAA               TT^CGAA
BstEII       3604  GGTGACC              G^GT[ACGT]ACC
BstPI        3604  GGTGACC              G^GT[ACGT]ACC
Bsu36I       1437  CCTAAGG              CC^T[ACGT]AGG
CelII        4486  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1072  GCCGGT               [GA]^CCGG[CT]
ClaI         2336  ATCGAT               AT^CGAT
DraI         2185  TTTAAA               TTT^AAA
DraIII       1424  CACTTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           90  GACTATGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         4282  CCACGG               C^C[GA][CT]GG
EaeI         2769  TGGCCA               [CT]^GGCC[GA]
Eco81I       1437  CCTAAGG              CC^T[ACGT]AGG
EcoNI        4596  CCTTTACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3604  GGTGACC              G^GT[ACGT]ACC
EcoRV        4177  GATATC               GAT^ATC
EcoT22I      1768  ATGCAT               ATGCA^T
FbaI         3896  TGATCA               T^GATCA
HaeII         208  AGCGCC               [GA]GCGC^[CT]
HgaI         1743  GACGC                GACGC(5/10)
HpaI         2155  GTTAAC               GTT^AAC
KpnI          868  GGTACC               GGTAC^C
MamI         2737  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2769  TGGCCA               TGG^CCA
MroI         3215  TCCGGA               T^CCGGA
MscI         2769  TGGCCA               TGG^CCA
NdeI         3284  CATATG               CA^TATG
NsiI         1768  ATGCAT               ATGCA^T
NspV          185  TTCGAA               TT^CGAA
PaeR7I       3491  CTCGAG               C^TCGAG
PflFI        2344  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3113  CACGTG               CAC^GTG
PmlI         3113  CACGTG               CAC^GTG
PshBI        2791  ATTAAT               AT^TAAT
SacI         3932  GAGCTC               GAGCT^C
SalI         2429  GTCGAC               G^TCGAC
SfuI          185  TTCGAA               TT^CGAA
SnaBI        1058  TACGTA               TAC^GTA
SpeI         3436  ACTAGT               A^CTAGT
SphI         1770  GCATGC               GCATG^C
SspI         4030  AATATT               AAT^ATT
StuI         1309  AGGCCT               AGG^CCT
Tth111I      2344  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4021  TCTAGA               T^CTAGA
XhoI         3491  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII        1745  CGCG                 CG^CG
             2707  CGCG                
AclI          639  AACGTT               AA^CGTT
             3299  AACGTT              
AflIII       3112  ACACGT               A^C[GA][CT]GT
             3114  ACGTGT              
Asp700I      3415  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3789  GAAAGAATTC          
BamHI        2146  GGATCC               G^GATCC
             2853  GGATCC              
BanII        1829  GGGCTC               G[GA]GC[CT]^C
             3932  GAGCTC              
BciVI        1525  GGATAC               GTATCC(6/5)
             1256  GTATCC              
BsaAI        1058  TACGTA               [CT]AC^GT[GA]
             3113  CACGTG              
BsaI         2901  GAGACC               GGTCTC(1/5)
             2128  GGTCTC              
BseRI        1235  CTCCTC               GAGGAG(10/8)
             2611  GAGGAG              
BspMI         871  ACCTGC               ACCTGC(4/8)
             3312  ACCTGC              
BstUI        1745  CGCG                 CG^CG
             2707  CGCG                
BstXI        2857  CCAGAATTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3594  CCAACAAGTTGG        
CfoI          209  GCGC                 GCG^C
             1774  GCGC                
HhaI          209  GCGC                 GCG^C
             1774  GCGC                
HinP1I        209  GCGC                 G^CGC
             1774  GCGC                
MspA1I        935  CAGCTG               C[AC]G^C[GT]G
             3716  CAGCTG              
MvnI         1745  CGCG                 CG^CG
             2707  CGCG                
NheI          226  GCTAGC               G^CTAGC
              595  GCTAGC              
Psp1406I      639  AACGTT               AA^CGTT
             3299  AACGTT              
PvuII         935  CAGCTG               CAG^CTG
             3716  CAGCTG              
SapI         1618  GAAGAGC              GCTCTTC(1/4)
             3889  GAAGAGC             
SexAI        2837  ACCTGGT              A^CC[AT]GGT
             2915  ACCAGGT             
Tsp45I        322  GTCAC                ^GT[GC]AC
             3605  GTGAC               
XcmI         4144  CCAACTGATGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4953  CCAGGCACCTTTTGG     
XmnI         3415  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3789  GAAAGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BanI        
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
Bsp1407I    BspHI       BsrDI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        RcaI        RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com