Restriction map for pF1KA0034
 (Circular) (Six-base) MAPSORT of: pF1KA0034.seq  Check: 3038  from: 1  to: 8138



pF1KA0034  8138 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:24  ..

AccI GT'mk_AC

Cuts at:   2538    5176    2538 
   Size:       2638    5500
 
Acc65I G'GTAC_C

Cuts at:    976    5154     976 
   Size:       4178    3960
 
AclI AA'CG_TT

Cuts at:    748    3408    7978     748 
   Size:       2660    4570     908

  Fragments arranged by size: 

                4570    2660     908
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    171     355    1208    1336    1411    1508    1837    1856    2603 
   Size:        184     853     128      75      97     329      19     747

Cuts at:   2603    3083    3329    4778    4937    5963    6395    6748    7410 
   Size:        480     246    1449     159    1026     432     353     662

Cuts at:   7410     171 
   Size:        899

  Fragments arranged by size: 

                1449    1026     899     853     747     662     480     432
                 353     329     246     184     159     128      97      75
                  19
 
AflII C'TTAA_G

Cuts at:   2395    2395 
   Size:       8138
 
AflIII A'CryG_T

Cuts at:   3220    3222    5490    7290    7571    3220 
   Size:          2    2268    1800     281    3787

  Fragments arranged by size: 

                3787    2268    1800     281       2
 
AleI CACnn'nnGTG

Cuts at:   3809    4406    3809 
   Size:        597    7541
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1811    3001    1811 
   Size:       1190    6948
 
AlwNI CAG_nnn'CTG

Cuts at:    120     159    2142    2199    3079    4545    5443    6881     120 
   Size:         39    1983      57     880    1466     898    1438    1377

  Fragments arranged by size: 

                1983    1466    1438    1377     898     880      57      39
            
 
ApaLI G'TGCA_C

Cuts at:   2287    6976    2287 
   Size:       4689    3449
 
ApoI r'AATT_y

Cuts at:    214    1207    1277    2388    2702    2916    3328    3670    3901 
   Size:        993      70    1111     314     214     412     342     231

Cuts at:   3901    4084    4345    5142    7491    7747     214 
   Size:        183     261     797    2349     256     605

  Fragments arranged by size: 

                2349    1111     993     797     605     412     342     314
                 261     256     231     214     183      70
 
AseI AT'TA_AT

Cuts at:     20    2900      20 
   Size:       2880    5258
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8138
 
AvaI C'yCGr_G

Cuts at:   3599    5158    3599 
   Size:       1559    6579
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    147     180    1347    1380     147 
   Size:         33    1167      33    6905

  Fragments arranged by size: 

                6905    1167      33      33
 
BamHI G'GATC_C

Cuts at:   2254    2961    5163    2254 
   Size:        707    2202    5229

  Fragments arranged by size: 

                5229    2202     707
 
BanI G'GyrC_C

Cuts at:    557     976    5064    5154    5568    5816    5851     557 
   Size:        419    4088      90     414     248      35    2844

  Fragments arranged by size: 

                4088    2844     419     414     248      90      35
 
BanII G_rGCy'C

Cuts at:   1941    4044    5152    6182    1941 
   Size:       2103    1108    1030    3897

  Fragments arranged by size: 

                3897    2103    1108    1030
 
BbeI G_GCGC'C

Cuts at:   5572    5820    5572 
   Size:        248    7890
 
BbsI GAAGACnn'nnnn_

Cuts at:    303     849    2096     303 
   Size:        546    1247    6345

  Fragments arranged by size: 

                6345    1247     546
 
BbvCI CC'TCA_GC

Cuts at:   2648    2648 
   Size:       8138
 
BciVI GTATCCnnnnn_n'

Cuts at:   1375    1627    6036    7092    8138    1375 
   Size:        252    4409    1056    1046    1375

  Fragments arranged by size: 

                4409    1375    1056    1046     252
 
BclI T'GATC_A

Cuts at:   4004    5658    4004 
   Size:       1654    6484
 
BfrBI ATG'CAT

Cuts at:   1878    1878 
   Size:       8138
 
BglII A'GATC_T

Cuts at:   5653    7690    5653 
   Size:       2037    6101
 
BlpI GC'TnA_GC

Cuts at:   4595    5250    4595 
   Size:        655    7483
 
Bme1580I G_kGCm'C

Cuts at:   2291    5763    5856    6980    2291 
   Size:       3472      93    1124    3449

  Fragments arranged by size: 

                3472    3449    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1504    2330    3444    3564    4036    5013    5036    5350    5431 
   Size:        826    1114     120     472     977      23     314      81

Cuts at:   5431    5766    7910    1504 
   Size:        335    2144    1732

  Fragments arranged by size: 

                2144    1732    1114     977     826     472     335     314
                 120      81      23
 
BmtI G_CTAG'C

Cuts at:    338     707     338 
   Size:        369    7769
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6505    6505 
   Size:       8138
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    130     136     516     800     851    1274    1625    3151    3450 
   Size:          6     380     284      51     423     351    1526     299

Cuts at:   3450    4135     130 
   Size:        685    4133

  Fragments arranged by size: 

                4133    1526     685     423     380     351     299     284
                  51       6
 
Bpu10I CC'TnA_GC

Cuts at:   2375    2648    2742    2375 
   Size:        273      94    7771

  Fragments arranged by size: 

                7771     273      94
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    988    1871    1882    4054    5314    6193    6907    7205     988 
   Size:        883      11    2172    1260     879     714     298    1921

  Fragments arranged by size: 

                2172    1921    1260     883     879     714     298      11
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   2242    3003    7842    2242 
   Size:        761    4839    2538

  Fragments arranged by size: 

                4839    2538     761
 
BsaAI yAC'GTr

Cuts at:   1168    3223    5491    1168 
   Size:       2055    2268    3815

  Fragments arranged by size: 

                3815    2268    2055
 
BsaBI GATnn'nnATC

Cuts at:      6    2849    5652    7689       6 
   Size:       2843    2803    2037     455

  Fragments arranged by size: 

                2843    2803    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   5569    5817    6519    8015    5569 
   Size:        248     702    1496    5692

  Fragments arranged by size: 

                5692    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   3323    5327    5848    6526    6937    7084    7358    3323 
   Size:       2004     521     678     411     147     274    4103

  Fragments arranged by size: 

                4103    2004     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1444    1474    1444 
   Size:         30    8108
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1334    2734    1334 
   Size:       1400    6738
 
BseYI C'CCAG_C

Cuts at:    171     358    1320    1325    5563    6321    6986    7394     171 
   Size:        187     962       5    4238     758     665     408     915

  Fragments arranged by size: 

                4238     962     915     758     665     408     187       5
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    605    1010    2042    4430     605 
   Size:        405    1032    2388    4313

  Fragments arranged by size: 

                4313    2388    1032     405
 
BsiEI CG_ry'CG

Cuts at:    104    5680    5726    6956     104 
   Size:       5576      46    1230    1286

  Fragments arranged by size: 

                5576    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   2291    2474    4044    4112    5152    5930    6120    6980    2291 
   Size:        183    1570      68    1040     778     190     860    3449

  Fragments arranged by size: 

                3449    1570    1040     860     778     190     183      68
            
 
BsmI GAATG_Cn'

Cuts at:   1798    1798 
   Size:       8138
 
Bsp1286I G_dGCh'C

Cuts at:   1941    2291    2474    4044    4112    5152    5763    5856    5930 
   Size:        350     183    1570      68    1040     611      93      74

Cuts at:   5930    6120    6182    6980    1941 
   Size:        190      62     798    3099

  Fragments arranged by size: 

                3099    1570    1040     798     611     350     190     183
                  93      74      68      62
 
BspEI T'CCGG_A

Cuts at:   3323    3323 
   Size:       8138
 
BspHI T'CATG_A

Cuts at:    323    1032    2175    2706    3092    4913    6570     323 
   Size:        709    1143     531     386    1821    1657    1891

  Fragments arranged by size: 

                1891    1821    1657    1143     709     531     386
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    988    3429    5188    5402    5704    6085     988 
   Size:       2441    1759     214     302     381    3041

  Fragments arranged by size: 

                3041    2441    1759     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    6430    6484    7968    8133      50 
   Size:       6380      54    1484     165      55

  Fragments arranged by size: 

                6380    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    902    2916    3297    3420    4724    6050     902 
   Size:       2014     381     123    1304    1326    2990

  Fragments arranged by size: 

                2990    2014    1326    1304     381     123
 
BsrFI r'CCGG_y

Cuts at:   1180    6136    6317    1180 
   Size:       4956     181    3001

  Fragments arranged by size: 

                4956    3001     181
 
BsrGI T'GTAC_A

Cuts at:   2517    3030    3758    3939    2517 
   Size:        513     728     181    6716

  Fragments arranged by size: 

                6716     728     513     181
 
BssSI C'ACGA_G

Cuts at:   4406    6409    7117    4406 
   Size:       2003     708    5427

  Fragments arranged by size: 

                5427    2003     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   5515    5515 
   Size:       8138
 
BstBI TT'CG_AA

Cuts at:    294    6499     294 
   Size:       6205    1933
 
BstEII G'GTnAC_C

Cuts at:   3712    3712 
   Size:       8138
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2972    3709    2972 
   Size:        737    7401
 
BstYI r'GATC_y

Cuts at:      1     446    2254    2844    2961    5163    5630    5653    5988 
   Size:        445    1808     590     117    2202     467      23     335

Cuts at:   5988    6234    6540    6552    6638    7690       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                2202    1808    1052     590     467     449     445     335
                 306     246     117      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1546    1546 
   Size:       8138
 
BtgI C'CryG_G

Cuts at:    107    4390     107 
   Size:       4283    3855
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   6088    6269    6432    6088 
   Size:        181     163    7794

  Fragments arranged by size: 

                7794     181     163
 
BtsI GCAGTG_nn'

Cuts at:    854    1795    2030    3197    4777    5381     854 
   Size:        941     235    1167    1580     604    3611

  Fragments arranged by size: 

                3611    1580    1167     941     604     235
 
ClaI AT'CG_AT

Cuts at:   2445    2445 
   Size:       8138
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   3528    3563    3528 
   Size:         35    8103
 
DraI TTT'AAA

Cuts at:   2295    5137    2295 
   Size:       2842    5296
 
DraIII CAC_nnn'GTG

Cuts at:   1537    1537 
   Size:       8138
 
DrdI GACnn_nn'nnGTC

Cuts at:    204    5844    7188     204 
   Size:       5640    1344    1154

  Fragments arranged by size: 

                5640    1344    1154
 
EaeI y'GGCC_r

Cuts at:   2877    5723    5897    6288    6315    7660    2877 
   Size:       2846     174     391      27    1345    3355

  Fragments arranged by size: 

                3355    2846    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   5723    5723 
   Size:       8138
 
EarI CTCTTCn'nnn_

Cuts at:    374    1721    2833    2929    3992    4654    6161    6371     374 
   Size:       1347    1112      96    1063     662    1507     210    2141

  Fragments arranged by size: 

                2141    1507    1347    1112    1063     662     210      96
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   6346    7088    7234    7948    6346 
   Size:        742     146     714    6536

  Fragments arranged by size: 

                6536     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   4042    5150    4042 
   Size:       1108    7030
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    130     136     171     355     516     800     851    1208    1274 
   Size:          6      35     184     161     284      51     357      66

Cuts at:   1274    1336    1411    1508    1625    1837    1856    2603    3083 
   Size:         62      75      97     117     212      19     747     480

Cuts at:   3083    3151    3329    3450    4135    4778    4937    5963    6395 
   Size:         68     178     121     685     643     159    1026     432

Cuts at:   6395    6748    7410     130 
   Size:        353     662     858

  Fragments arranged by size: 

                1026     858     747     685     662     643     480     432
                 357     353     284     212     184     178     161     159
                 121     117      97      75      68      66      62      51
                  35      19       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:   4708    4708 
   Size:       8138
 
EcoO109I rG'GnC_Cy

Cuts at:   1840    2277    3057    5038    5277    1840 
   Size:        437     780    1981     239    4701

  Fragments arranged by size: 

                4701    1981     780     437     239
 
EcoRI G'AATT_C

Cuts at:   1277    2702    2916    3901    4345    5142    1277 
   Size:       1425     214     985     444     797    4273

  Fragments arranged by size: 

                4273    1425     985     797     444     214
 
EcoRV GAT'ATC

Cuts at:   4287    4287 
   Size:       8138
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1750    3017    4192    4720    4941    6157    1750 
   Size:       1267    1175     528     221    1216    3731

  Fragments arranged by size: 

                3731    1267    1216    1175     528     221
 
FspI TGC'GCA

Cuts at:   5919    5919 
   Size:       8138
 
HaeII r_GCGC'y

Cuts at:    320    5572    5820    7050    7821     320 
   Size:       5252     248    1230     771     637

  Fragments arranged by size: 

                5252    1230     771     637     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   3496    3528    4233    4265    4570    4602    5980    6010    6012 
   Size:         32     705      32     305      32    1378      30       2

Cuts at:   6012    6042    3496 
   Size:         30    5592

  Fragments arranged by size: 

                5592    1378     705     305      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2064    2189    2265    2539    2574    3436    5177    2064 
   Size:        125      76     274      35     862    1741    5025

  Fragments arranged by size: 

                5025    1741     862     274     125      76      35
 
HpaI GTT'AAC

Cuts at:   2265    2265 
   Size:       8138
 
KasI G'GCGC_C

Cuts at:   5568    5816    5568 
   Size:        248    7890
 
KpnI G_GTAC'C

Cuts at:    980    5158     980 
   Size:       4178    3960
 
MluI A'CGCG_T

Cuts at:   7571    7571 
   Size:       8138
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1611    2802    3726    3995    4276    4376    6897    7081    1611 
   Size:       1191     924     269     281     100    2521     184    2668

  Fragments arranged by size: 

                2668    2521    1191     924     281     269     184     100
            
 
MscI TGG'CCA

Cuts at:   2879    5899    2879 
   Size:       3020    5118
 
MslI CAynn'nnrTG

Cuts at:    268     388     959     988    3809    3947    4051    4406    6127 
   Size:        120     571      29    2821     138     104     355    1721

Cuts at:   6127    6254     268 
   Size:        127    2152

  Fragments arranged by size: 

                2821    2152    1721     571     355     138     127     120
                 104      29
 
MspA1I CmG'CkG

Cuts at:   1045    3826    5249    5563    5923    6707    6952    1045 
   Size:       2781    1423     314     360     784     245    2231

  Fragments arranged by size: 

                2781    2231    1423     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   6319    6319 
   Size:       8138
 
NarI GG'CG_CC

Cuts at:   5569    5817    5569 
   Size:        248    7890
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       8138
 
NdeI CA'TA_TG

Cuts at:   3393    3393 
   Size:       8138
 
NgoMIV G'CCGG_C

Cuts at:   6317    6317 
   Size:       8138
 
NheI G'CTAG_C

Cuts at:    334     703     334 
   Size:        369    7769
 
NsiI A_TGCA'T

Cuts at:   1880    1880 
   Size:       8138
 
NspI r_CATG'y

Cuts at:    700    1882    3946    5191    5691    7294     700 
   Size:       1182    2064    1245     500    1603    1544

  Fragments arranged by size: 

                2064    1603    1544    1245    1182     500
 
PasI CC'CwG_GG

Cuts at:   1227    1227 
   Size:       8138
 
PciI A'CATG_T

Cuts at:   7290    7290 
   Size:       8138
 
PflMI CCAn_nnn'nTGG

Cuts at:    159    2466    5098    5113     159 
   Size:       2307    2632      15    3184

  Fragments arranged by size: 

                3184    2632    2307      15
 
PmeI GTTT'AAAC

Cuts at:   5137    5137 
   Size:       8138
 
PmlI CAC'GTG

Cuts at:   3223    3223 
   Size:       8138
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6584    6616    6584 
   Size:         32    8106
 
PpuMI rG'GwC_Cy

Cuts at:   1840    2277    3057    5038    1840 
   Size:        437     780    1981    4940

  Fragments arranged by size: 

                4940    1981     780     437
 
PstI C_TGCA'G

Cuts at:   1979    2147    3102    4164    4772    5185    5870    1979 
   Size:        168     955    1062     608     413     685    4247

  Fragments arranged by size: 

                4247    1062     955     685     608     413     168
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       8138
 
PvuII CAG'CTG

Cuts at:   1045    3826    5563    5923    1045 
   Size:       2781    1737     360    3260

  Fragments arranged by size: 

                3260    2781    1737     360
 
RsrII CG'GwC_CG

Cuts at:   6333    6333 
   Size:       8138
 
SacI G_AGCT'C

Cuts at:   4044    5152    4044 
   Size:       1108    7030
 
SalI G'TCGA_C

Cuts at:   2537    5175    2537 
   Size:       2638    5500
 
SapI GCTCTTCn'nnn_

Cuts at:   1721    3992    6161    6371    1721 
   Size:       2271    2169     210    3488

  Fragments arranged by size: 

                3488    2271    2169     210
 
SbfI CC_TGCA'GG

Cuts at:   5185    5185 
   Size:       8138
 
ScaI AGT'ACT

Cuts at:   1357    3574    3855    4924    7655    1357 
   Size:       2217     281    1069    2731    1840

  Fragments arranged by size: 

                2731    2217    1840    1069     281
 
SexAI A'CCwGG_T

Cuts at:   2945    3023    2945 
   Size:         78    8060
 
SfcI C'TryA_G

Cuts at:     33     662     818    1975    2143    2349    2546    3098    4160 
   Size:        629     156    1157     168     206     197     552    1062

Cuts at:   4160    4320    4768    4985    5181    5866    6834    7025      33 
   Size:        160     448     217     196     685     968     191    1146

  Fragments arranged by size: 

                1157    1146    1062     968     685     629     552     448
                 217     206     197     196     191     168     160     156
            
 
SfoI GGC'GCC

Cuts at:   5570    5818    5570 
   Size:        248    7890
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8138
 
SmaI CCC'GGG

Cuts at:   5160    5160 
   Size:       8138
 
SmlI C'TyrA_G

Cuts at:    967    1861    1886    2395    3599    4033    5293    6208    6922 
   Size:        894      25     509    1204     434    1260     915     714

Cuts at:   6922    7184     967 
   Size:        262    1921

  Fragments arranged by size: 

                1921    1260    1204     915     894     714     509     434
                 262      25
 
SnaBI TAC'GTA

Cuts at:   1168    1168 
   Size:       8138
 
SpeI A'CTAG_T

Cuts at:     67    3544      67 
   Size:       3477    4661
 
SphI G_CATG'C

Cuts at:   1882    5191    5691    1882 
   Size:       3309     500    4329

  Fragments arranged by size: 

                4329    3309     500
 
SspI AAT'ATT

Cuts at:   4140    4140 
   Size:       8138
 
StuI AGG'CCT

Cuts at:   1419    1419 
   Size:       8138
 
StyI C'CwwG_G

Cuts at:    107    2686    4145    4295    5272    5626     107 
   Size:       2579    1459     150     977     354    2619

  Fragments arranged by size: 

                2619    2579    1459     977     354     150
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1486    2050    3799    5721    5854    6313    6522    1486 
   Size:        564    1749    1922     133     459     209    3102

  Fragments arranged by size: 

                3102    1922    1749     564     459     209     133
 
TatI w'GTAC_w

Cuts at:   1355    1422    1792    2517    3030    3572    3758    3853    3939 
   Size:         67     370     725     513     542     186      95      86

Cuts at:   3939    4922    7653    1355 
   Size:        983    2731    1840

  Fragments arranged by size: 

                2731    1840     983     725     542     513     370     186
                  95      86      67
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4662    4694    7655    7687    4662 
   Size:         32    2961      32    5113

  Fragments arranged by size: 

                5113    2961      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2455    5935    2455 
   Size:       3480    4658
 
XbaI T'CTAG_A

Cuts at:   4129    5169    4129 
   Size:       1040    7098
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   4259    5068    5633    4259 
   Size:        809     565    6764

  Fragments arranged by size: 

                6764     809     565
 
XhoI C'TCGA_G

Cuts at:   3599    3599 
   Size:       8138
 
XmaI C'CCGG_G

Cuts at:   5158    5158 
   Size:       8138
 
XmnI GAAnn'nnTTC

Cuts at:   3527    3901    3527 
   Size:        374    7764
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AleI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NsiI     NspI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AgeI     AhdI     AloI     ApaI     AscI
    AvrII     BcgI     BglI    BmgBI    BsiWI    BsmBI   BssHII  BstZ17I
     FseI    FspAI  HindIII     MfeI     NotI     NruI     PacI     PfoI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI
    SgrAI     SrfI     SwaI     ZraI
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