Restriction map for pF1KB7323
##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AscI        AseI        AsnI        Asp718I     
AviII       AvrII       BfrI        BglII       BlnI        
BsaBI       BsiWI       BspLU11I    Bst1107I    BstBI       
BstZ17I     CpoI        DraI        EcoRI       EcoT22I     
FseI        FspI        HindIII     HpaI        KpnI        
MamI        MfeI        MluI        MunI        NdeI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        SbfI        SfiI        
SfuI        SnaBI       Sse8387I    SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccIII       5417  TCCGGA               T^CCGGA
AflIII       2804  ACGTGT               A^C[GA][CT]GT
AhdI           67  GACGAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1739  AGCGCT               AGC^GCT
AspEI          67  GACGAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3337  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1172  GGATCC               G^GATCC
BcgI          939  CGAATGCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        5179  GTATCC               GTATCC(6/5)
BclI         1832  TGATCA               T^GATCA
BsaI         4450  GAGACC               GGTCTC(1/5)
BseAI        5417  TCCGGA               T^CCGGA
BsiEI         812  CGGCCG               CG[GA][CT]^CG
BsmI          940  GAATGC               GAATGC(1/-1)
Bsp1407I     5765  TGTACA               T^GTACA
BspDI        1876  ATCGAT               AT^CGAT
BspEI        5417  TCCGGA               T^CCGGA
BspHI        2954  TCATGA               T^CATGA
BsrGI        5765  TGTACA               T^GTACA
BssHII       2264  GCGCGC               G^CGCGC
BssSI        4822  CACGAG               CACGAG(-5/-1)
ClaI         1876  ATCGAT               AT^CGAT
DrdI         2041  GACCCCACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          812  CGGCCG               C^GGCCG
Eam1105I       67  GACGAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         812  CGGCCG               C^GGCCG
Eco47III     1739  AGCGCT               AGC^GCT
Eco52I        812  CGGCCG               C^GGCCG
EcoRV        5857  GATATC               GAT^ATC
FbaI         1832  TGATCA               T^GATCA
MroI         5417  TCCGGA               T^CCGGA
NaeI         2820  GCCGGC               GCC^GGC
NgoMIV       2820  GCCGGC               G^CCGGC
NheI          675  GCTAGC               G^CTAGC
PaeR7I        867  CTCGAG               C^TCGAG
PflFI        3337  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         2954  TCATGA               T^CATGA
ScaI         2873  AGTACT               AGT^ACT
SgrAI        5209  CACCGGCG             C[GA]^CCGG[CT]G
SpeI         4524  ACTAGT               A^CTAGT
SphI         4319  GCATGC               GCATG^C
SspBI        5765  TGTACA               T^GTACA
SspI         4112  AATATT               AAT^ATT
Tth111I      3337  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          867  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AcsI         2452  AAATTC               [GA]^AATT[CT]
             5098  AAATTC              
Alw44I       1822  GTGCAC               G^TGCAC
             1863  GTGCAC              
AocI          501  CCTCAGG              CC^T[ACGT]AGG
             4591  CCTGAGG             
ApaLI        1822  GTGCAC               G^TGCAC
             1863  GTGCAC              
ApoI         2452  AAATTC               [GA]^AATT[CT]
             5098  AAATTC              
Asp700I      2418  GAAGGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5355  GAACAAGTTC          
BalI         3326  TGGCCA               TGG^CCA
             3482  TGGCCA              
BbeI         2313  GGCGCC               GGCGC^C
             5213  GGCGCC              
BbrPI        2458  CACGTG               CAC^GTG
             4495  CACGTG              
BbvCI        1995  GCTGAGG              CCTCAGC(-5/-2)
             5014  GCTGAGG             
BfaI          676  CTAG                 C^TAG
             4525  CTAG                
BsaWI        3800  ACCGGA               [AT]^CCGG[AT]
             5417  TCCGGA              
BsmBI         652  GAGACG               CGTCTC(1/5)
             4870  GAGACG              
BstEII        657  GGTCACC              G^GT[ACGT]ACC
             2331  GGTCACC             
BstPI         657  GGTCACC              G^GT[ACGT]ACC
             2331  GGTCACC             
Bsu36I        501  CCTCAGG              CC^T[ACGT]AGG
             4591  CCTGAGG             
Eco81I        501  CCTCAGG              CC^T[ACGT]AGG
             4591  CCTGAGG             
EcoO65I       657  GGTCACC              G^GT[ACGT]ACC
             2331  GGTCACC             
KasI         2313  GGCGCC               G^GCGCC
             5213  GGCGCC              
KspI           34  CCGCGG               CCGC^GG
              614  CCGCGG              
MaeI          676  CTAG                 C^TAG
             4525  CTAG                
MluNI        3326  TGGCCA               TGG^CCA
             3482  TGGCCA              
MscI         3326  TGGCCA               TGG^CCA
             3482  TGGCCA              
NarI         2313  GGCGCC               GG^CGCC
             5213  GGCGCC              
NcoI         2068  CCATGG               C^CATGG
             5465  CCATGG              
PmaCI        2458  CACGTG               CAC^GTG
             4495  CACGTG              
PmlI         2458  CACGTG               CAC^GTG
             4495  CACGTG              
PstI          947  CTGCAG               CTGCA^G
             5674  CTGCAG              
SacII          34  CCGCGG               CCGC^GG
              614  CCGCGG              
SapI          963  GAAGAGC              GCTCTTC(1/4)
             4374  GAAGAGC             
SexAI        2255  ACCTGGT              A^CC[AT]GGT
             3509  ACCTGGT             
SfoI         2313  GGCGCC               GGC^GCC
             5213  GGCGCC              
Tsp509I      2453  AATT                 ^AATT
             5099  AATT                
XcmI         1351  CCAAACGGTGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3554  CCAAGCAGGAGCTGG     
XmnI         2418  GAAGGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5355  GAACAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XhoII       XmaCI       XmaI        
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