Restriction map for pF1KB7323
 (Linear) (Six-base) MAPSORT of: KIBB7323.seq  Check: 9118  from: 1  to: 5862



KIBB7323.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     684    1087    1325    3295    3468    5862 
   Size:        684     403     238    1970     173    2394

  Fragments arranged by size: 

                2394    1970     684     403     238     173
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     474    1414    2620    3050    3118    3375    4219    5510 
   Size:        474     940    1206     430      68     257     844    1291

Cuts at:   5510    5862 
   Size:        352

  Fragments arranged by size: 

                1291    1206     940     844     474     430     352     257
                  68
 
AfeI AGC'GCT

Cuts at:      0    1741    5862 
   Size:       1741    4121
 
AflIII A'CryG_T

Cuts at:      0    2804    5862 
   Size:       2804    3058
 
AhdI GACnn_n'nnGTC

Cuts at:      0      72    5862 
   Size:         72    5790
 
AleI CACnn'nnGTG

Cuts at:      0    3478    4424    4648    5862 
   Size:       3478     946     224    1214

  Fragments arranged by size: 

                3478    1214     946     224
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2212    3514    5862 
   Size:       2212    1302    2348

  Fragments arranged by size: 

                2348    2212    1302
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     430     462    2368    2400    2551    2583    2641    2673 
   Size:        430      32    1906      32     151      32      58      32

Cuts at:   2673    5862 
   Size:       3189

  Fragments arranged by size: 

                3189    1906     430     151      58      32      32      32
                  32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3564    4767    5523    5862 
   Size:       3564    1203     756     339

  Fragments arranged by size: 

                3564    1203     756     339
 
ApaI G_GGCC'C

Cuts at:      0      10    2148    2252    3273    4601    5786    5800    5846 
   Size:         10    2138     104    1021    1328    1185      14      46

Cuts at:   5846    5862 
   Size:         16

  Fragments arranged by size: 

                2138    1328    1185    1021     104      46      16      14
                  10
 
ApaLI G'TGCA_C

Cuts at:      0    1822    1863    5862 
   Size:       1822      41    3999

  Fragments arranged by size: 

                3999    1822      41
 
ApoI r'AATT_y

Cuts at:      0    2452    5098    5862 
   Size:       2452    2646     764

  Fragments arranged by size: 

                2646    2452     764
 
AvaI C'yCGr_G

Cuts at:      0     399     867    1083    1132    1342    3265    3299    3449 
   Size:        399     468     216      49     210    1923      34     150

Cuts at:   3449    4238    4472    4600    4741    4850    5862 
   Size:        789     234     128     141     109    1012

  Fragments arranged by size: 

                1923    1012     789     468     399     234     216     210
                 150     141     128     109      49      34
 
BamHI G'GATC_C

Cuts at:      0    1172    5862 
   Size:       1172    4690
 
BanI G'GyrC_C

Cuts at:      0      16     565     696    2313    3119    3974    4418    5207 
   Size:         16     549     131    1617     806     855     444     789

Cuts at:   5207    5213    5862 
   Size:          6     649

  Fragments arranged by size: 

                1617     855     806     789     649     549     444     131
                  16       6
 
BanII G_rGCy'C

Cuts at:      0      10     131     392     821    1439    1484    2148    2252 
   Size:         10     121     261     429     618      45     664     104

Cuts at:   2252    2483    2512    2837    3273    3859    4094    4601    4831 
   Size:        231      29     325     436     586     235     507     230

Cuts at:   4831    4851    5030    5312    5786    5800    5846    5862 
   Size:         20     179     282     474      14      46      16

  Fragments arranged by size: 

                 664     618     586     507     474     436     429     325
                 282     261     235     231     230     179     121     104
                  46      45      29      20      16      14      10
 
BbeI G_GCGC'C

Cuts at:      0    2317    5217    5862 
   Size:       2317    2900     645

  Fragments arranged by size: 

                2900    2317     645
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1688    1958    2747    3056    5862 
   Size:       1688     270     789     309    2806

  Fragments arranged by size: 

                2806    1688     789     309     270
 
BbvCI CC'TCA_GC

Cuts at:      0    1996    5015    5862 
   Size:       1996    3019     847

  Fragments arranged by size: 

                3019    1996     847
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     928     962    5862 
   Size:        928      34    4900

  Fragments arranged by size: 

                4900     928      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    5190    5862 
   Size:       5190     672
 
BclI T'GATC_A

Cuts at:      0    1832    5862 
   Size:       1832    4030
 
BglI GCCn_nnn'nGGC

Cuts at:      0      14    1971    3013    3268    4164    5485    5841    5862 
   Size:         14    1957    1042     255     896    1321     356      21

  Fragments arranged by size: 

                1957    1321    1042     896     356     255      21      14
            
 
BlpI GC'TnA_GC

Cuts at:      0    2854    4957    5602    5862 
   Size:       2854    2103     645     260

  Fragments arranged by size: 

                2854    2103     645     260
 
Bme1580I G_kGCm'C

Cuts at:      0      10      19     701    1826    1867    2148    2155    2252 
   Size:         10       9     682    1125      41     281       7      97

Cuts at:   2252    3122    3273    4472    4601    4655    4749    5786    5800 
   Size:        870     151    1199     129      54      94    1037      14

Cuts at:   5800    5846    5862 
   Size:         46      16

  Fragments arranged by size: 

                1199    1125    1037     870     682     281     151     129
                  97      94      54      46      41      16      14      10
                   9       7
 
BmgBI CAC'GTC

Cuts at:      0     435    1476    2805    3612    3744    5622    5862 
   Size:        435    1041    1329     807     132    1878     240

  Fragments arranged by size: 

                1878    1329    1041     807     435     240     132
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       4    1932    2298    3966    5862 
   Size:          4    1928     366    1668    1896

  Fragments arranged by size: 

                1928    1896    1668     366       4
 
BmtI G_CTAG'C

Cuts at:      0     679    5862 
   Size:        679    5183
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2256    2376    4500    4644    5862 
   Size:       2256     120    2124     144    1218

  Fragments arranged by size: 

                2256    2124    1218     144     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     770    1141    1443    1528    1612    2134    2666    2668 
   Size:        770     371     302      85      84     522     532       2

Cuts at:   2668    2722    2734    3187    3199    4470    4972    5365    5407 
   Size:         54      12     453      12    1271     502     393      42

Cuts at:   5407    5611    5862 
   Size:        204     251

  Fragments arranged by size: 

                1271     770     532     522     502     453     393     371
                 302     251     204      85      84      54      42      12
                  12       2
 
Bpu10I CC'TnA_GC

Cuts at:      0    1499    1996    2785    3853    5015    5698    5862 
   Size:       1499     497     789    1068    1162     683     164

  Fragments arranged by size: 

                1499    1162    1068     789     683     497     164
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     292     805    1894    1963    2539    2779    3562 
   Size:        151     141     513    1089      69     576     240     783

Cuts at:   3562    5862 
   Size:       2300

  Fragments arranged by size: 

                2300    1089     783     576     513     240     151     141
                  69
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4444    5862 
   Size:       4444    1418
 
BsaAI yAC'GTr

Cuts at:      0    2328    2460    3879    4497    5862 
   Size:       2328     132    1419     618    1365

  Fragments arranged by size: 

                2328    1419    1365     618     132
 
BsaHI Gr'CG_yC

Cuts at:      0    1295    2314    4334    5214    5862 
   Size:       1295    1019    2020     880     648

  Fragments arranged by size: 

                2020    1295    1019     880     648
 
BsaWI w'CCGG_w

Cuts at:      0    3800    5417    5862 
   Size:       3800    1617     445

  Fragments arranged by size: 

                3800    1617     445
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     379     409     910     940    2065    2095    2365    2395 
   Size:        379      30     501      30    1125      30     270      30

Cuts at:   2395    2641    2671    3841    3871    4045    4075    5380    5410 
   Size:        246      30    1170      30     174      30    1305      30

Cuts at:   5410    5801    5831    5862 
   Size:        391      30      31

  Fragments arranged by size: 

                1305    1170    1125     501     391     379     270     246
                 174      31      30      30      30      30      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      37      40      64     469     855     904     987    1222 
   Size:         37       3      24     405     386      49      83     235

Cuts at:   1222    1225    1240    1243    1246    1296    1750    2656    2665 
   Size:          3      15       3       3      50     454     906       9

Cuts at:   2665    2731    3280    3478    3904    3919    3949    4620    4870 
   Size:         66     549     198     426      15      30     671     250

Cuts at:   4870    4882    4927    5032    5179    5422    5608    5745    5862 
   Size:         12      45     105     147     243     186     137     117

  Fragments arranged by size: 

                 906     671     549     454     426     405     386     250
                 243     235     198     186     147     137     117     105
                  83      66      50      49      45      37      30      24
                  15      15      12       9       3       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     701    1483    1944    2769    3272    4263    4313    4686 
   Size:        701     782     461     825     503     991      50     373

Cuts at:   4686    4712    4716    4830    5542    5862 
   Size:         26       4     114     712     320

  Fragments arranged by size: 

                 991     825     782     712     701     503     461     373
                 320     114      50      26       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2083    2218    2464    4466    5554    5862 
   Size:       2083     135     246    2002    1088     308

  Fragments arranged by size: 

                2083    2002    1088     308     246     135
 
BsiEI CG_ry'CG

Cuts at:      0     815    5862 
   Size:        815    5047
 
BsiHKAI G_wGCw'C

Cuts at:      0    1826    1867    2483    2837    3131    3859    4094    5862 
   Size:       1826      41     616     354     294     728     235    1768

  Fragments arranged by size: 

                1826    1768     728     616     354     294     235      41
            
 
BsmI GAATG_Cn'

Cuts at:      0     946    5862 
   Size:        946    4916
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     646    4864    5862 
   Size:        646    4218     998

  Fragments arranged by size: 

                4218     998     646
 
Bsp1286I G_dGCh'C

Cuts at:      0      10      19     131     392     701     821    1439    1484 
   Size:         10       9     112     261     309     120     618      45

Cuts at:   1484    1826    1867    2148    2155    2252    2483    2512    2837 
   Size:        342      41     281       7      97     231      29     325

Cuts at:   2837    3122    3131    3273    3859    4094    4472    4601    4655 
   Size:        285       9     142     586     235     378     129      54

Cuts at:   4655    4749    4831    4851    5030    5312    5786    5800    5846 
   Size:         94      82      20     179     282     474      14      46

Cuts at:   5846    5862 
   Size:         16

  Fragments arranged by size: 

                 618     586     474     378     342     325     309     285
                 282     281     261     235     231     179     142     129
                 120     112      97      94      82      54      46      45
                  41      29      20      16      14      10       9       9
                   7
 
BspEI T'CCGG_A

Cuts at:      0    5417    5862 
   Size:       5417     445
 
BspHI T'CATG_A

Cuts at:      0    2954    5862 
   Size:       2954    2908
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     684    1087    1325    1856    2774    3295    3468    4032 
   Size:        684     403     238     531     918     521     173     564

Cuts at:   4032    5363    5862 
   Size:       1331     499

  Fragments arranged by size: 

                1331     918     684     564     531     521     499     403
                 238     173
 
BsrBI CCG'CTC

Cuts at:      0    2360    3204    3915    4860    5169    5862 
   Size:       2360     844     711     945     309     693

  Fragments arranged by size: 

                2360     945     844     711     693     309
 
BsrDI GCAATG_nn'

Cuts at:      0     219     512    1320    1563    5862 
   Size:        219     293     808     243    4299

  Fragments arranged by size: 

                4299     808     293     243     219
 
BsrFI r'CCGG_y

Cuts at:      0    2820    3642    5210    5862 
   Size:       2820     822    1568     652

  Fragments arranged by size: 

                2820    1568     822     652
 
BsrGI T'GTAC_A

Cuts at:      0    5765    5862 
   Size:       5765      97
 
BssHII G'CGCG_C

Cuts at:      0    2264    5862 
   Size:       2264    3598
 
BssSI C'ACGA_G

Cuts at:      0    4822    5862 
   Size:       4822    1040
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2766    3126    4497    5862 
   Size:       2766     360    1371    1365

  Fragments arranged by size: 

                2766    1371    1365     360
 
BstEII G'GTnAC_C

Cuts at:      0     657    2331    5862 
   Size:        657    1674    3531

  Fragments arranged by size: 

                3531    1674     657
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1054    3156    3795    5862 
   Size:       1054    2102     639    2067

  Fragments arranged by size: 

                2102    2067    1054     639
 
BstYI r'GATC_y

Cuts at:      0     240     533    1172    1346    4937    5862 
   Size:        240     293     639     174    3591     925

  Fragments arranged by size: 

                3591     925     639     293     240     174
 
Bsu36I CC'TnA_GG

Cuts at:      0     502    4592    5862 
   Size:        502    4090    1270

  Fragments arranged by size: 

                4090    1270     502
 
BtgI C'CryG_G

Cuts at:      0      34     614    2045    2068    3008    3329    5287    5465 
   Size:         34     580    1431      23     940     321    1958     178

Cuts at:   5465    5862 
   Size:        397

  Fragments arranged by size: 

                1958    1431     940     580     397     321     178      34
                  23
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    5103    5862 
   Size:       5103     759
 
BtsI GCAGTG_nn'

Cuts at:      0     276    2095    3762    5862 
   Size:        276    1819    1667    2100

  Fragments arranged by size: 

                2100    1819    1667     276
 
ClaI AT'CG_AT

Cuts at:      0    1877    5862 
   Size:       1877    3985
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1303    1338    5862 
   Size:       1303      35    4524

  Fragments arranged by size: 

                4524    1303      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1608    3126    3513    5153    5862 
   Size:       1608    1518     387    1640     709

  Fragments arranged by size: 

                1640    1608    1518     709     387
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2047    5862 
   Size:       2047    3815
 
EaeI y'GGCC_r

Cuts at:      0     413     812    2168    2822    3326    3411    3482    3689 
   Size:        413     399    1356     654     504      85      71     207

Cuts at:   3689    3779    4861    5663    5862 
   Size:         90    1082     802     199

  Fragments arranged by size: 

                1356    1082     802     654     504     413     399     207
                 199      90      85      71
 
EagI C'GGCC_G

Cuts at:      0     812    5862 
   Size:        812    5050
 
EarI CTCTTCn'nnn_

Cuts at:      0     230     242     958     982    1199    1693    2998    3604 
   Size:        230      12     716      24     217     494    1305     606

Cuts at:   3604    4145    4195    4369    4414    5862 
   Size:        541      50     174      45    1448

  Fragments arranged by size: 

                1448    1305     716     606     541     494     230     217
                 174      50      45      24      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3349    4453    5862 
   Size:       3349    1104    1409

  Fragments arranged by size: 

                3349    1409    1104
 
EcoICRI GAG'CTC

Cuts at:      0    2481    2835    3857    4092    5862 
   Size:       2481     354    1022     235    1770

  Fragments arranged by size: 

                2481    1770    1022     354     235
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     474     770    1141    1414    1443    1528    1612    2134 
   Size:        474     296     371     273      29      85      84     522

Cuts at:   2134    2620    2666    2668    2722    2734    3050    3118    3187 
   Size:        486      46       2      54      12     316      68      69

Cuts at:   3187    3199    3375    4219    4470    4972    5365    5407    5510 
   Size:         12     176     844     251     502     393      42     103

Cuts at:   5510    5611    5862 
   Size:        101     251

  Fragments arranged by size: 

                 844     522     502     486     474     393     371     316
                 296     273     251     251     176     103     101      85
                  84      69      68      54      46      42      29      12
                  12       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1083    1912    2227    3166    3235    4018    5527    5572 
   Size:       1083     829     315     939      69     783    1509      45

Cuts at:   5572    5862 
   Size:        290

  Fragments arranged by size: 

                1509    1083     939     829     783     315     290      69
                  45
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6     423     745    1088    1468    1713    2118    2248 
   Size:          6     417     322     343     380     245     405     130

Cuts at:   2248    2249    2627    3015    3228    3269    3999    4362    4479 
   Size:          1     378     388     213      41     730     363     117

Cuts at:   4479    4597    4779    4881    5448    5577    5724    5796    5797 
   Size:        118     182     102     567     129     147      72       1

Cuts at:   5797    5842    5843    5862 
   Size:         45       1      19

  Fragments arranged by size: 

                 730     567     417     405     388     380     378     363
                 343     322     245     213     182     147     130     129
                 118     117     102      72      45      41      19       6
                   1       1       1
 
EcoRV GAT'ATC

Cuts at:      0    5859    5862 
   Size:       5859       3
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     846    5862 
   Size:        846    5016
 
HaeII r_GCGC'y

Cuts at:      0     590     911    1743    2317    5217    5747    5862 
   Size:        590     321     832     574    2900     530     115

  Fragments arranged by size: 

                2900     832     590     574     530     321     115
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      58      90     208     240     379     411    1654    1686 
   Size:         58      32     118      32     139      32    1243      32

Cuts at:   1686    3034    3066    3652    3684    4078    4090    4110    4122 
   Size:       1348      32     586      32     394      12      20      12

Cuts at:   4122    5801    5833    5862 
   Size:       1679      32      29

  Fragments arranged by size: 

                1679    1348    1243     586     394     139     118      58
                  32      32      32      32      32      32      32      29
                  20      12      12
 
HincII GTy'rAC

Cuts at:      0    4047    4560    5820    5862 
   Size:       4047     513    1260      42

  Fragments arranged by size: 

                4047    1260     513      42
 
KasI G'GCGC_C

Cuts at:      0    2313    5213    5862 
   Size:       2313    2900     649

  Fragments arranged by size: 

                2900    2313     649
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     875    5663    5862 
   Size:        236     639    4788     199

  Fragments arranged by size: 

                4788     639     236     199
 
MscI TGG'CCA

Cuts at:      0    3328    3484    5862 
   Size:       3328     156    2378

  Fragments arranged by size: 

                3328    2378     156
 
MslI CAynn'nnrTG

Cuts at:      0    1609    2956    3478    3980    4258    4424    4648    5862 
   Size:       1609    1347     522     502     278     166     224    1214

  Fragments arranged by size: 

                1609    1347    1214     522     502     278     224     166
            
 
MspA1I CmG'CkG

Cuts at:      0      36     616     625    1338    1410    1590    2112    4197 
   Size:         36     580       9     713      72     180     522    2085

Cuts at:   4197    4284    4686    4716    4902    5264    5589    5862 
   Size:         87     402      30     186     362     325     273

  Fragments arranged by size: 

                2085     713     580     522     402     362     325     273
                 186     180      87      72      36      30       9
 
NaeI GCC'GGC

Cuts at:      0    2822    5862 
   Size:       2822    3040
 
NarI GG'CG_CC

Cuts at:      0    2314    5214    5862 
   Size:       2314    2900     648

  Fragments arranged by size: 

                2900    2314     648
 
NcoI C'CATG_G

Cuts at:      0    2068    5465    5862 
   Size:       2068    3397     397

  Fragments arranged by size: 

                3397    2068     397
 
NgoMIV G'CCGG_C

Cuts at:      0    2820    5862 
   Size:       2820    3042
 
NheI G'CTAG_C

Cuts at:      0     675    5862 
   Size:        675    5187
 
NspI r_CATG'y

Cuts at:      0    2784    2820    4323    4558    5862 
   Size:       2784      36    1503     235    1304

  Fragments arranged by size: 

                2784    1503    1304     235      36
 
PasI CC'CwG_GG

Cuts at:      0     954    2148    5838    5862 
   Size:        954    1194    3690      24

  Fragments arranged by size: 

                3690    1194     954      24
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2519    3513    4353    5862 
   Size:       2519     994     840    1509

  Fragments arranged by size: 

                2519    1509     994     840
 
PfoI T'CCnGG_A

Cuts at:      0    2711    3164    3176    4186    4940    5862 
   Size:       2711     453      12    1010     754     922

  Fragments arranged by size: 

                2711    1010     922     754     453      12
 
PmlI CAC'GTG

Cuts at:      0    2460    4497    5862 
   Size:       2460    2037    1365

  Fragments arranged by size: 

                2460    2037    1365
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1513    1545    2641    2673    5862 
   Size:       1513      32    1096      32    3189

  Fragments arranged by size: 

                3189    1513    1096      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1713    2627    3999    4362    5448    5577    5724    5862 
   Size:       1713     914    1372     363    1086     129     147     138

  Fragments arranged by size: 

                1713    1372    1086     914     363     147     138     129
            
 
PspOMI G'GGCC_C

Cuts at:      0       6    2144    2248    3269    4597    5782    5796    5842 
   Size:          6    2138     104    1021    1328    1185      14      46

Cuts at:   5842    5862 
   Size:         20

  Fragments arranged by size: 

                2138    1328    1185    1021     104      46      20      14
                   6
 
PspXI vC'TCGA_Gb

Cuts at:      0     867    5862 
   Size:        867    4995
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2866    2898    5862 
   Size:       2866      32    2964

  Fragments arranged by size: 

                2964    2866      32
 
PstI C_TGCA'G

Cuts at:      0     951    5678    5862 
   Size:        951    4727     184

  Fragments arranged by size: 

                4727     951     184
 
PvuII CAG'CTG

Cuts at:      0    1590    2112    4197    4716    4902    5589    5862 
   Size:       1590     522    2085     519     186     687     273

  Fragments arranged by size: 

                2085    1590     687     522     519     273     186
 
SacI G_AGCT'C

Cuts at:      0    2483    2837    3859    4094    5862 
   Size:       2483     354    1022     235    1768

  Fragments arranged by size: 

                2483    1768    1022     354     235
 
SacII CC_GC'GG

Cuts at:      0      37     617    5862 
   Size:         37     580    5245

  Fragments arranged by size: 

                5245     580      37
 
SanDI GG'GwC_CC

Cuts at:      0    2627    5724    5862 
   Size:       2627    3097     138

  Fragments arranged by size: 

                3097    2627     138
 
SapI GCTCTTCn'nnn_

Cuts at:      0     958    4369    5862 
   Size:        958    3411    1493

  Fragments arranged by size: 

                3411    1493     958
 
ScaI AGT'ACT

Cuts at:      0    2875    5862 
   Size:       2875    2987
 
SexAI A'CCwGG_T

Cuts at:      0    2255    3509    5862 
   Size:       2255    1254    2353

  Fragments arranged by size: 

                2353    2255    1254
 
SfcI C'TryA_G

Cuts at:      0     647     947    1585    3085    3815    3832    5674    5862 
   Size:        647     300     638    1500     730      17    1842     188

  Fragments arranged by size: 

                1842    1500     730     647     638     300     188      17
            
 
SfoI GGC'GCC

Cuts at:      0    2315    5215    5862 
   Size:       2315    2900     647

  Fragments arranged by size: 

                2900    2315     647
 
SgrAI Cr'CCGG_yG

Cuts at:      0    5210    5862 
   Size:       5210     652
 
SmaI CCC'GGG

Cuts at:      0    3267    3301    4474    4743    5862 
   Size:       3267      34    1173     269    1119

  Fragments arranged by size: 

                3267    1173    1119     269      34
 
SmlI C'TyrA_G

Cuts at:      0     166     307     820     867    1909    1978    2554    2794 
   Size:        166     141     513      47    1042      69     576     240

Cuts at:   2794    3577    5862 
   Size:        783    2285

  Fragments arranged by size: 

                2285    1042     783     576     513     240     166     141
                  69      47
 
SpeI A'CTAG_T

Cuts at:      0    4524    5862 
   Size:       4524    1338
 
SphI G_CATG'C

Cuts at:      0    4323    5862 
   Size:       4323    1539
 
SspI AAT'ATT

Cuts at:      0    4114    5862 
   Size:       4114    1748
 
StuI AGG'CCT

Cuts at:      0     487    1918    3388    4291    5862 
   Size:        487    1431    1470     903    1571

  Fragments arranged by size: 

                1571    1470    1431     903     487
 
StyI C'CwwG_G

Cuts at:      0     731    2068    2735    2918    4756    4793    5009    5465 
   Size:        731    1337     667     183    1838      37     216     456

Cuts at:   5465    5528    5862 
   Size:         63     334

  Fragments arranged by size: 

                1838    1337     731     667     456     334     216     183
                  63      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     553    1563    2845    3520    3666    5862 
   Size:        553    1010    1282     675     146    2196

  Fragments arranged by size: 

                2196    1282    1010     675     553     146
 
TatI w'GTAC_w

Cuts at:      0    1378    2873    4568    5765    5862 
   Size:       1378    1495    1695    1197      97

  Fragments arranged by size: 

                1695    1495    1378    1197      97
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     163     195     910     942    3198    3230    4063    4095 
   Size:        163      32     715      32    2256      32     833      32

Cuts at:   4095    5862 
   Size:       1767

  Fragments arranged by size: 

                2256    1767     833     715     163      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3340    5862 
   Size:       3340    2522
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1358    3561    5862 
   Size:       1358    2203    2301

  Fragments arranged by size: 

                2301    2203    1358
 
XhoI C'TCGA_G

Cuts at:      0     867    5862 
   Size:        867    4995
 
XmaI C'CCGG_G

Cuts at:      0    3265    3299    4472    4741    5862 
   Size:       3265      34    1173     269    1121

  Fragments arranged by size: 

                3265    1173    1121     269      34
 
XmnI GAAnn'nnTTC

Cuts at:      0    2422    5359    5862 
   Size:       2422    2937     503

  Fragments arranged by size: 

                2937    2422     503
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NspI     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI
   PspOMI    PspXI     PsrI     PstI    PvuII     SacI    SacII    SanDI
     SapI     ScaI    SexAI     SfcI     SfoI    SgrAI     SmaI     SmlI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AscI     AseI
    AsiSI    AvrII     BaeI    BfrBI    BglII    BsaBI    BsiWI    BstBI
  BstZ17I     DraI    EcoRI     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     NdeI     NotI     NruI     NsiI     PacI
     PciI     PmeI    PshAI     PsiI     PvuI    RsrII     SalI     SbfI
     SfiI     SgfI    SnaBI     SrfI     SwaI     XbaI     ZraI
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