Restriction map for pF1KE0032
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AgeI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AviII       AvrII       BbrPI       BciVI       BclI        
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        HindIII     
KpnI        KspI        MamI        MfeI        MluI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Van91I      XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          476  GTCTAC               GT^[AC][GT]AC
AccIII        290  TCCGGA               T^CCGGA
AcyI          340  GGCGCC               G[GA]^CG[CT]C
AhdI          541  GACTCCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         1481  GGGCCC               GGGCC^C
AspEI         541  GACTCCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1786  GAGCTC               G[AT]GC[AT]^C
AspI          850  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1013  TGGCCA               TGG^CCA
BbeI          340  GGCGCC               GGCGC^C
BcgI          201  GCACAGACTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI         340  GGCGCC               G[GA]^CG[CT]C
BsaI          175  GAGACC               GGTCTC(1/5)
BsaWI         290  TCCGGA               [AT]^CCGG[AT]
BseAI         290  TCCGGA               T^CCGGA
BsiHKAI      1786  GAGCTC               G[AT]GC[AT]^C
BspEI         290  TCCGGA               T^CCGGA
BspHI        1250  TCATGA               T^CATGA
BsrBI          15  CCGCTC               CCGCTC(-3/-3)
BsrDI         978  CATTGC               GCAATG(2/0)
BssSI         885  CACGAG               CACGAG(-5/-1)
BstAPI       1689  GCAATACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1348  GGTGACC              G^GT[ACGT]ACC
BstPI        1348  GGTGACC              G^GT[ACGT]ACC
BstXI        1004  CCACGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI            3  GACTGCTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      541  GACTCCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1348  GGTGACC              G^GT[ACGT]ACC
Hin1I         340  GGCGCC               G[GA]^CG[CT]C
HpaI         1317  GTTAAC               GTT^AAC
KasI          340  GGCGCC               G^GCGCC
MaeII         698  ACGT                 A^CGT
MluNI        1013  TGGCCA               TGG^CCA
MroI          290  TCCGGA               T^CCGGA
MscI         1013  TGGCCA               TGG^CCA
NarI          340  GGCGCC               GG^CGCC
PaeR7I       1789  CTCGAG               C^TCGAG
PflFI         850  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         482  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI         1250  TCATGA               T^CATGA
SacI         1786  GAGCTC               GAGCT^C
ScaI         1610  AGTACT               AGT^ACT
SfoI          340  GGCGCC               GGC^GCC
StuI         1289  AGGCCT               AGG^CCT
Tth111I       850  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1789  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         215  CGCG                 CG^CG
              225  CGCG                
BamHI         626  GGATCC               G^GATCC
             1386  GGATCC              
BanI          340  GGCGCC               G^G[CT][GA]CC
             1154  GGCACC              
BbsI          894  GTCTTC               GAAGAC(2/6)
             1141  GTCTTC              
BbvCI         567  CCTCAGC              CCTCAGC(-5/-2)
             1356  GCTGAGG             
BfaI          394  CTAG                 C^TAG
             1199  CTAG                
BpmI         1747  CTCCAG               CTGGAG(16/14)
              752  CTGGAG              
BpuAI         894  GTCTTC               GAAGAC(2/6)
             1141  GTCTTC              
BstUI         215  CGCG                 CG^CG
              225  CGCG                
EcoRI         371  GAATTC               G^AATTC
             1225  GAATTC              
HaeII         340  GGCGCC               [GA]GCGC^[CT]
             1764  GGCGCT              
MaeI          394  CTAG                 C^TAG
             1199  CTAG                
MvnI          215  CGCG                 CG^CG
              225  CGCG                
NaeI          161  GCCGGC               GCC^GGC
              582  GCCGGC              
NgoMIV        161  GCCGGC               G^CCGGC
              582  GCCGGC              
PstI         1306  CTGCAG               CTGCA^G
             1696  CTGCAG              
SfaNI         572  GCATC                GCATC(5/9)
             1509  GCATC               
SmaI          126  CCCGGG               CCC^GGG
             1478  CCCGGG              
SmlI         1525  CTCAAG               C^T[CT][GA]AG
             1789  CTCGAG              
TaqI         1676  TCGA                 T^CGA
             1790  TCGA                
TfiI          430  GAATC                G^A[AT]TC
             1089  GATTC               
TthHB8I      1676  TCGA                 T^CGA
             1790  TCGA                
XmaCI         126  CCCGGG               C^CCGGG
             1478  CCCGGG              
XmaI          126  CCCGGG               C^CCGGG
             1478  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaI        AvaII       BanII       
BbvI        BcnI        BglI        BlpI        BmyI        
Bpu1102I    BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        StyI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       
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