Restriction map for pF1KB8567
 (Circular) (Six-base) MAPSORT of: pF1KB8567.seq  Check: 5498  from: 1  to: 4307



pF1KB8567  4307 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AccI GT'mk_AC

Cuts at:   1345    1345 
   Size:       4307
 
Acc65I G'GTAC_C

Cuts at:   1323    1323 
   Size:       4307
 
AclI AA'CG_TT

Cuts at:   4147    4147 
   Size:       4307
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1249    2132    2564    2917    3579    1249 
   Size:        883     432     353     662    1977

  Fragments arranged by size: 

                1977     883     662     432     353
 
AflII C'TTAA_G

Cuts at:    915     915 
   Size:       4307
 
AflIII A'CryG_T

Cuts at:    671    1659    3459    3740     671 
   Size:        988    1800     281    1238

  Fragments arranged by size: 

                1800    1238     988     281
 
AlwNI CAG_nnn'CTG

Cuts at:   1039    1612    3050    1039 
   Size:        573    1438    2296

  Fragments arranged by size: 

                2296    1438     573
 
ApaLI G'TGCA_C

Cuts at:   3145    3145 
   Size:       4307
 
ApoI r'AATT_y

Cuts at:    880    1186    1311    3660    3916     880 
   Size:        306     125    2349     256    1271

  Fragments arranged by size: 

                2349    1271     306     256     125
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4307
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4307
 
AvaI C'yCGr_G

Cuts at:   1327    1327 
   Size:       4307
 
BamHI G'GATC_C

Cuts at:   1332    1332 
   Size:       4307
 
BanI G'GyrC_C

Cuts at:   1323    1737    1985    2020    1323 
   Size:        414     248      35    3610

  Fragments arranged by size: 

                3610     414     248      35
 
BanII G_rGCy'C

Cuts at:   1321    2351    1321 
   Size:       1030    3277
 
BbeI G_GCGC'C

Cuts at:   1741    1989    1741 
   Size:        248    4059
 
BbsI GAAGACnn'nnnn_

Cuts at:    806     806 
   Size:       4307
 
BbvCI CC'TCA_GC

Cuts at:    491    1247     491 
   Size:        756    3551
 
BciVI GTATCCnnnnn_n'

Cuts at:   1102    2205    3261    4307    1102 
   Size:       1103    1056    1046    1102

  Fragments arranged by size: 

                1103    1102    1056    1046
 
BclI T'GATC_A

Cuts at:   1827    1827 
   Size:       4307
 
BglII A'GATC_T

Cuts at:   1822    3859    1822 
   Size:       2037    2270
 
BlpI GC'TnA_GC

Cuts at:   1419    1419 
   Size:       4307
 
Bme1580I G_kGCm'C

Cuts at:   1932    2025    3149    1932 
   Size:         93    1124    3090

  Fragments arranged by size: 

                3090    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1519    1600    1935    4079    1519 
   Size:         81     335    2144    1747

  Fragments arranged by size: 

                2144    1747     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    417    2674     417 
   Size:       2257    2050
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    562     562 
   Size:       4307
 
Bpu10I CC'TnA_GC

Cuts at:    491    1247     491 
   Size:        756    3551
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1483    2362    3076    3374    1483 
   Size:        879     714     298    2416

  Fragments arranged by size: 

                2416     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4011    4011 
   Size:       4307
 
BsaAI yAC'GTr

Cuts at:   1660    1660 
   Size:       4307
 
BsaBI GATnn'nnATC

Cuts at:      6     521    1821    3858       6 
   Size:        515    1300    2037     455

  Fragments arranged by size: 

                2037    1300     515     455
 
BsaHI Gr'CG_yC

Cuts at:    123    1738    1986    2688    4184     123 
   Size:       1615     248     702    1496     246

  Fragments arranged by size: 

                1615    1496     702     248     246
 
BsaWI w'CCGG_w

Cuts at:   1496    2017    2695    3106    3253    3527    1496 
   Size:        521     678     411     147     274    2276

  Fragments arranged by size: 

                2276     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    652     682    1264    1294     652 
   Size:         30     582      30    3665

  Fragments arranged by size: 

                3665     582      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    790     790 
   Size:       4307
 
BseYI C'CCAG_C

Cuts at:   1732    2490    3155    3563    1732 
   Size:        758     665     408    2476

  Fragments arranged by size: 

                2476     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104    1849    1895    3125     104 
   Size:       1745      46    1230    1286

  Fragments arranged by size: 

                1745    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1321    2099    2289    3149    1321 
   Size:        778     190     860    2479

  Fragments arranged by size: 

                2479     860     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1321    1932    2025    2099    2289    2351    3149    1321 
   Size:        611      93      74     190      62     798    2479

  Fragments arranged by size: 

                2479     798     611     190      93      74      62
 
BspHI T'CATG_A

Cuts at:   2739    2739 
   Size:       4307
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1357    1571    1873    2254    1357 
   Size:        214     302     381    3410

  Fragments arranged by size: 

                3410     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2599    2653    4137    4302      50 
   Size:       2549      54    1484     165      55

  Fragments arranged by size: 

                2549    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2219    2219 
   Size:       4307
 
BsrFI r'CCGG_y

Cuts at:   2305    2486    2305 
   Size:        181    4126
 
BsrGI T'GTAC_A

Cuts at:    668     668 
   Size:       4307
 
BssSI C'ACGA_G

Cuts at:   2578    3286    2578 
   Size:        708    3599
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1039    1684    1039 
   Size:        645    3662
 
BstBI TT'CG_AA

Cuts at:   2668    2668 
   Size:       4307
 
BstXI CCAn_nnnn'nTGG

Cuts at:    144     615    1158     144 
   Size:        471     543    3293

  Fragments arranged by size: 

                3293     543     471
 
BstYI r'GATC_y

Cuts at:      1    1332    1799    1822    2157    2403    2709    2721    2807 
   Size:       1331     467      23     335     246     306      12      86

Cuts at:   2807    3859       1 
   Size:       1052     449

  Fragments arranged by size: 

                1331    1052     467     449     335     306     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    392     392 
   Size:       4307
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4307
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1176    2257    2438    2601    1176 
   Size:       1081     181     163    2882

  Fragments arranged by size: 

                2882    1081     181     163
 
BtsI GCAGTG_nn'

Cuts at:    507    1550     507 
   Size:       1043    3264
 
ClaI AT'CG_AT

Cuts at:    693     693 
   Size:       4307
 
DraI TTT'AAA

Cuts at:   1306    1306 
   Size:       4307
 
DrdI GACnn_nn'nnGTC

Cuts at:   2013    3357    2013 
   Size:       1344    2963
 
EaeI y'GGCC_r

Cuts at:   1148    1892    2066    2457    2484    3829    1148 
   Size:        744     174     391      27    1345    1626

  Fragments arranged by size: 

                1626    1345     744     391     174      27
 
EagI C'GGCC_G

Cuts at:   1892    1892 
   Size:       4307
 
EarI CTCTTCn'nnn_

Cuts at:    407     576    2330    2540     407 
   Size:        169    1754     210    2174

  Fragments arranged by size: 

                2174    1754     210     169
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2515    3257    3403    4117    2515 
   Size:        742     146     714    2705

  Fragments arranged by size: 

                2705     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1319    1319 
   Size:       4307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    562    1249    2132    2564    2917    3579     562 
   Size:        687     883     432     353     662    1290

  Fragments arranged by size: 

                1290     883     687     662     432     353
 
EcoO109I rG'GnC_Cy

Cuts at:    376    1446     376 
   Size:       1070    3237
 
EcoRI G'AATT_C

Cuts at:   1311    1311 
   Size:       4307
 
EcoRV GAT'ATC

Cuts at:    519     519 
   Size:       4307
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2326    2326 
   Size:       4307
 
FspI TGC'GCA

Cuts at:   2088    2088 
   Size:       4307
 
HaeII r_GCGC'y

Cuts at:    115    1741    1989    3219    3990     115 
   Size:       1626     248    1230     771     432

  Fragments arranged by size: 

                1626    1230     771     432     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    735     767     771     803    1264    1296    2149    2179    2181 
   Size:         32       4      32     461      32     853      30       2

Cuts at:   2181    2211     735 
   Size:         30    2831

  Fragments arranged by size: 

                2831     853     461      32      32      32      30      30
                   4       2
 
HincII GTy'rAC

Cuts at:    312    1346     312 
   Size:       1034    3273
 
HindIII A'AGCT_T

Cuts at:    428     727     428 
   Size:        299    4008
 
KasI G'GCGC_C

Cuts at:   1737    1985    1737 
   Size:        248    4059
 
KpnI G_GTAC'C

Cuts at:   1327    1327 
   Size:       4307
 
MluI A'CGCG_T

Cuts at:   3740    3740 
   Size:       4307
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    144    3066    3250     144 
   Size:       2922     184    1201

  Fragments arranged by size: 

                2922    1201     184
 
MscI TGG'CCA

Cuts at:   1150    2068    1150 
   Size:        918    3389
 
MslI CAynn'nnrTG

Cuts at:    613    2296    2423     613 
   Size:       1683     127    2497

  Fragments arranged by size: 

                2497    1683     127
 
MspA1I CmG'CkG

Cuts at:    540    1039    1251    1418    1732    2092    2876    3121     540 
   Size:        499     212     167     314     360     784     245    1726

  Fragments arranged by size: 

                1726     784     499     360     314     245     212     167
            
 
NaeI GCC'GGC

Cuts at:   2488    2488 
   Size:       4307
 
NarI GG'CG_CC

Cuts at:   1738    1986    1738 
   Size:        248    4059
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4307
 
NgoMIV G'CCGG_C

Cuts at:   2486    2486 
   Size:       4307
 
NspI r_CATG'y

Cuts at:    603     675    1360    1860    3463     603 
   Size:         72     685     500    1603    1447

  Fragments arranged by size: 

                1603    1447     685     500      72
 
PciI A'CATG_T

Cuts at:    671    3459     671 
   Size:       2788    1519
 
PfoI T'CCnGG_A

Cuts at:    718     718 
   Size:       4307
 
PmeI GTTT'AAAC

Cuts at:   1306    1306 
   Size:       4307
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2753    2785    2753 
   Size:         32    4275
 
PpuMI rG'GwC_Cy

Cuts at:    376     376 
   Size:       4307
 
PstI C_TGCA'G

Cuts at:    502     659    1354    2039     502 
   Size:        157     695     685    2770

  Fragments arranged by size: 

                2770     695     685     157
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4307
 
PvuII CAG'CTG

Cuts at:    540    1039    1251    1732    2092     540 
   Size:        499     212     481     360    2755

  Fragments arranged by size: 

                2755     499     481     360     212
 
RsrII CG'GwC_CG

Cuts at:   2502    2502 
   Size:       4307
 
SacI G_AGCT'C

Cuts at:   1321    1321 
   Size:       4307
 
SalI G'TCGA_C

Cuts at:   1344    1344 
   Size:       4307
 
SapI GCTCTTCn'nnn_

Cuts at:   2330    2540    2330 
   Size:        210    4097
 
SbfI CC_TGCA'GG

Cuts at:   1354    1354 
   Size:       4307
 
ScaI AGT'ACT

Cuts at:   3824    3824 
   Size:       4307
 
SfcI C'TryA_G

Cuts at:     33     498     655    1350    2035    3003    3194      33 
   Size:        465     157     695     685     968     191    1146

  Fragments arranged by size: 

                1146     968     695     685     465     191     157
 
SfoI GGC'GCC

Cuts at:   1739    1987    1739 
   Size:        248    4059
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4307
 
SmaI CCC'GGG

Cuts at:   1329    1329 
   Size:       4307
 
SmlI C'TyrA_G

Cuts at:    915    1462    2377    3091    3353     915 
   Size:        547     915     714     262    1869

  Fragments arranged by size: 

                1869     915     714     547     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4307
 
SphI G_CATG'C

Cuts at:    603    1360    1860     603 
   Size:        757     500    3050

  Fragments arranged by size: 

                3050     757     500
 
SspI AAT'ATT

Cuts at:    153     795     153 
   Size:        642    3665
 
StyI C'CwwG_G

Cuts at:    107    1441    1795     107 
   Size:       1334     354    2619

  Fragments arranged by size: 

                2619    1334     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1890    2023    2482    2691    1890 
   Size:        133     459     209    3506

  Fragments arranged by size: 

                3506     459     209     133
 
TatI w'GTAC_w

Cuts at:    348     668    3822     348 
   Size:        320    3154     833

  Fragments arranged by size: 

                3154     833     320
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    264     296    3824    3856     264 
   Size:         32    3528      32     715

  Fragments arranged by size: 

                3528     715      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    132    2104     132 
   Size:       1972    2335
 
XbaI T'CTAG_A

Cuts at:   1338    1338 
   Size:       4307
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    975    1802     975 
   Size:        827    3480
 
XmaI C'CCGG_G

Cuts at:   1327    1327 
   Size:       4307
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI
    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AgeI     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BsgI    BsiWI     BsmI    BsmBI    BspEI   BssHII   BstEII
  BstZ17I    CspCI   DraIII    EcoNI     FseI    FspAI     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI
     XmnI     ZraI
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