Restriction map for pF1KB6722
 (Circular) (Six-base) MAPSORT of: pF1KB6722.seq  Check: 6091  from: 1  to: 3515



pF1KB6722  3515 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    553     553 
   Size:       3515
 
Acc65I G'GTAC_C

Cuts at:    531     531 
   Size:       3515
 
AclI AA'CG_TT

Cuts at:   3355    3355 
   Size:       3515
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1340    1772    2519    2787    1340 
   Size:        432     747     268    2068

  Fragments arranged by size: 

                2068     747     432     268
 
AfeI AGC'GCT

Cuts at:    313     313 
   Size:       3515
 
AflIII A'CryG_T

Cuts at:    867    1971    2948     867 
   Size:       1104     977    1434

  Fragments arranged by size: 

                1434    1104     977
 
AgeI A'CCGG_T

Cuts at:   1903    1903 
   Size:       3515
 
AlwNI CAG_nnn'CTG

Cuts at:    120     363     375     820    2387     120 
   Size:        243      12     445    1567    1248

  Fragments arranged by size: 

                1567    1248     445     243      12
 
ApaLI G'TGCA_C

Cuts at:   2285    2285 
   Size:       3515
 
ApoI r'AATT_y

Cuts at:    519    2868    3124     519 
   Size:       2349     256     910

  Fragments arranged by size: 

                2349     910     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3515
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3515
 
AvaI C'yCGr_G

Cuts at:    535     535 
   Size:       3515
 
BamHI G'GATC_C

Cuts at:    540     540 
   Size:       3515
 
BanI G'GyrC_C

Cuts at:    531     945    1193    1228     531 
   Size:        414     248      35    2818

  Fragments arranged by size: 

                2818     414     248      35
 
BanII G_rGCy'C

Cuts at:    192     350     463     529    1559     192 
   Size:        158     113      66    1030    2148

  Fragments arranged by size: 

                2148    1030     158     113      66
 
BbeI G_GCGC'C

Cuts at:    949    1197     949 
   Size:        248    3267
 
BciVI GTATCCnnnnn_n'

Cuts at:   1413    2174    3515    1413 
   Size:        761    1341    1413

  Fragments arranged by size: 

                1413    1341     761
 
BclI T'GATC_A

Cuts at:   1035    1035 
   Size:       3515
 
BglII A'GATC_T

Cuts at:   1030    3067    1030 
   Size:       2037    1478
 
BlpI GC'TnA_GC

Cuts at:    627     627 
   Size:       3515
 
Bme1580I G_kGCm'C

Cuts at:   1140    1233    2289    1140 
   Size:         93    1056    2366

  Fragments arranged by size: 

                2366    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    727     808    1143    3287     727 
   Size:         81     335    2144     955

  Fragments arranged by size: 

                2144     955     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1882    1882 
   Size:       3515
 
Bpu10I CC'TnA_GC

Cuts at:    191     270     191 
   Size:         79    3436
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    190     691    1570    2062    2360     190 
   Size:        501     879     492     298    1345

  Fragments arranged by size: 

                1345     879     501     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3219    3219 
   Size:       3515
 
BsaAI yAC'GTr

Cuts at:    868     868 
   Size:       3515
 
BsaBI GATnn'nnATC

Cuts at:      6    1029    3066       6 
   Size:       1023    2037     455

  Fragments arranged by size: 

                2037    1023     455
 
BsaHI Gr'CG_yC

Cuts at:    946    1194    1896    3392     946 
   Size:        248     702    1496    1069

  Fragments arranged by size: 

                1496    1069     702     248
 
BsaWI w'CCGG_w

Cuts at:    251     704    1225    1903    2177    2324    2735     251 
   Size:        453     521     678     274     147     411    1031

  Fragments arranged by size: 

                1031     678     521     453     411     274     147
 
BseYI C'CCAG_C

Cuts at:    285     940    1698    2275    2771     285 
   Size:        655     758     577     496    1029

  Fragments arranged by size: 

                1029     758     655     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    353     376     353 
   Size:         23    3492
 
BsiEI CG_ry'CG

Cuts at:    104    1057    1103    2311     104 
   Size:        953      46    1208    1308

  Fragments arranged by size: 

                1308    1208     953      46
 
BsiHKAI G_wGCw'C

Cuts at:    350     529    1307    1497    2289     350 
   Size:        179     778     190     792    1576

  Fragments arranged by size: 

                1576     792     778     190     179
 
Bsp1286I G_dGCh'C

Cuts at:    192     350     463     529    1140    1233    1307    1497    1559 
   Size:        158     113      66     611      93      74     190      62

Cuts at:   1559    2289     192 
   Size:        730    1418

  Fragments arranged by size: 

                1418     730     611     190     158     113      93      74
                  66      62
 
BspEI T'CCGG_A

Cuts at:   2735    2735 
   Size:       3515
 
BspHI T'CATG_A

Cuts at:   2691    2691 
   Size:       3515
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    565     779    1081    1462     565 
   Size:        214     302     381    2618

  Fragments arranged by size: 

                2618     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     486    1807    1861    3345    3510      50 
   Size:        436    1321      54    1484     165      55

  Fragments arranged by size: 

                1484    1321     436     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1427    1427 
   Size:       3515
 
BsrFI r'CCGG_y

Cuts at:   1513    1694    1903    1513 
   Size:        181     209    3125

  Fragments arranged by size: 

                3125     209     181
 
BssSI C'ACGA_G

Cuts at:   1786    2144    1786 
   Size:        358    3157
 
BstAPI GCAn_nnn'nTGC

Cuts at:    366     892     366 
   Size:        526    2989
 
BstBI TT'CG_AA

Cuts at:   1876    1876 
   Size:       3515
 
BstYI r'GATC_y

Cuts at:      1     540    1007    1030    1365    1611    2623    2709    2721 
   Size:        539     467      23     335     246    1012      86      12

Cuts at:   2721    3067       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     539     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3515
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1465    1646    1809    1465 
   Size:        181     163    3171

  Fragments arranged by size: 

                3171     181     163
 
BtsI GCAGTG_nn'

Cuts at:    183     758     183 
   Size:        575    2940
 
DraI TTT'AAA

Cuts at:    514     514 
   Size:       3515
 
DrdI GACnn_nn'nnGTC

Cuts at:    486    1221    2079     486 
   Size:        735     858    1922

  Fragments arranged by size: 

                1922     858     735
 
EaeI y'GGCC_r

Cuts at:   1100    1274    1665    1692    3037    1100 
   Size:        174     391      27    1345    1578

  Fragments arranged by size: 

                1578    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1100    1100 
   Size:       3515
 
EarI CTCTTCn'nnn_

Cuts at:   1538    1748    1538 
   Size:        210    3305
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1723    2033    2179    3325    1723 
   Size:        310     146    1146    1913

  Fragments arranged by size: 

                1913    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    348     527     348 
   Size:        179    3336
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1340    1772    2519    2787    1340 
   Size:        432     747     268    2068

  Fragments arranged by size: 

                2068     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    167     431     654     167 
   Size:        264     223    3028

  Fragments arranged by size: 

                3028     264     223
 
EcoRI G'AATT_C

Cuts at:    519     519 
   Size:       3515
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1534    1534 
   Size:       3515
 
FspI TGC'GCA

Cuts at:   1296    1296 
   Size:       3515
 
HaeII r_GCGC'y

Cuts at:    315     949    1197    2219    3198     315 
   Size:        634     248    1022     979     632

  Fragments arranged by size: 

                1022     979     634     632     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    357     389    1357    1387    1389    1419     357 
   Size:         32     968      30       2      30    2453

  Fragments arranged by size: 

                2453     968      32      30      30       2
 
HincII GTy'rAC

Cuts at:    554     554 
   Size:       3515
 
HindIII A'AGCT_T

Cuts at:    267     267 
   Size:       3515
 
KasI G'GCGC_C

Cuts at:    945    1193     945 
   Size:        248    3267
 
KpnI G_GTAC'C

Cuts at:    535     535 
   Size:       3515
 
MluI A'CGCG_T

Cuts at:   2948    2948 
   Size:       3515
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2186    2370    2186 
   Size:        184    3331
 
MscI TGG'CCA

Cuts at:   1276    1276 
   Size:       3515
 
MslI CAynn'nnrTG

Cuts at:   1504    1631    1504 
   Size:        127    3388
 
MspA1I CmG'CkG

Cuts at:    363     626     940    1300    2313    2558     363 
   Size:        263     314     360    1013     245    1320

  Fragments arranged by size: 

                1320    1013     360     314     263     245
 
NaeI GCC'GGC

Cuts at:   1696    1696 
   Size:       3515
 
NarI GG'CG_CC

Cuts at:    946    1194     946 
   Size:        248    3267
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3515
 
NgoMIV G'CCGG_C

Cuts at:   1694    1694 
   Size:       3515
 
NspI r_CATG'y

Cuts at:    568    1068    1975     568 
   Size:        500     907    2108

  Fragments arranged by size: 

                2108     907     500
 
PasI CC'CwG_GG

Cuts at:    408     408 
   Size:       3515
 
PciI A'CATG_T

Cuts at:   1971    1971 
   Size:       3515
 
PmeI GTTT'AAAC

Cuts at:    514     514 
   Size:       3515
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2654    2686    2654 
   Size:         32    3483
 
PpuMI rG'GwC_Cy

Cuts at:    167     167 
   Size:       3515
 
PstI C_TGCA'G

Cuts at:    418     562    1247     418 
   Size:        144     685    2686

  Fragments arranged by size: 

                2686     685     144
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3515
 
PvuII CAG'CTG

Cuts at:    363     940    1300     363 
   Size:        577     360    2578

  Fragments arranged by size: 

                2578     577     360
 
RsrII CG'GwC_CG

Cuts at:   1710    1710 
   Size:       3515
 
SacI G_AGCT'C

Cuts at:    350     529     350 
   Size:        179    3336
 
SalI G'TCGA_C

Cuts at:    552     552 
   Size:       3515
 
SapI GCTCTTCn'nnn_

Cuts at:   1538    1748    1538 
   Size:        210    3305
 
SbfI CC_TGCA'GG

Cuts at:    562     562 
   Size:       3515
 
ScaI AGT'ACT

Cuts at:   3032    3032 
   Size:       3515
 
SfcI C'TryA_G

Cuts at:     33     414     558    1243    2236    2427      33 
   Size:        381     144     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     381     191     144
 
SfoI GGC'GCC

Cuts at:    947    1195     947 
   Size:        248    3267
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3515
 
SmaI CCC'GGG

Cuts at:    537     537 
   Size:       3515
 
SmlI C'TyrA_G

Cuts at:    205     670    1585    2077    2339     205 
   Size:        465     915     492     262    1381

  Fragments arranged by size: 

                1381     915     492     465     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3515
 
SphI G_CATG'C

Cuts at:    568    1068     568 
   Size:        500    3015
 
StyI C'CwwG_G

Cuts at:    107     171     233     649    1003     107 
   Size:         64      62     416     354    2619

  Fragments arranged by size: 

                2619     416     354      64      62
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    342    1098    1231    1690    1899     342 
   Size:        756     133     459     209    1958

  Fragments arranged by size: 

                1958     756     459     209     133
 
TatI w'GTAC_w

Cuts at:    209    3030     209 
   Size:       2821     694
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3032    3064    3032 
   Size:         32    3483
 
Tth111I GACn'n_nGTC

Cuts at:   1312    1312 
   Size:       3515
 
XbaI T'CTAG_A

Cuts at:    546     546 
   Size:       3515
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    178     347    1010     178 
   Size:        169     663    2683

  Fragments arranged by size: 

                2683     663     169
 
XmaI C'CCGG_G

Cuts at:    535     535 
   Size:       3515
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI     PmeI
     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI    BsaXI    BseRI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
     XmnI     ZraI
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