Restriction map for pF1KB8435
 (Circular) (Six-base) MAPSORT of: pF1KB8435.seq  Check: 3170  from: 1  to: 4622



pF1KB8435  4622 bp

 With 182 enzymes: SgfI * 

                        August 7, 2008 14:13  ..

AccI GT'mk_AC

Cuts at:   1660    1660 
   Size:       4622
 
Acc65I G'GTAC_C

Cuts at:   1638    1638 
   Size:       4622
 
AclI AA'CG_TT

Cuts at:   4462    4462 
   Size:       4622
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    331     857     928    1349    2447    2879    3232    3894     331 
   Size:        526      71     421    1098     432     353     662    1059

  Fragments arranged by size: 

                1098    1059     662     526     432     421     353      71
            
 
AflIII A'CryG_T

Cuts at:   1434    1974    3774    4055    1434 
   Size:        540    1800     281    2001

  Fragments arranged by size: 

                2001    1800     540     281
 
AhdI GACnn_n'nnGTC

Cuts at:    199     807     199 
   Size:        608    4014
 
AleI CACnn'nnGTG

Cuts at:    118     118 
   Size:       4622
 
AlwNI CAG_nnn'CTG

Cuts at:    279     452     468     864    1927    3365     279 
   Size:        173      16     396    1063    1438    1536

  Fragments arranged by size: 

                1536    1438    1063     396     173      16
 
ApaLI G'TGCA_C

Cuts at:   1438    1576    3460    1438 
   Size:        138    1884    2600

  Fragments arranged by size: 

                2600    1884     138
 
ApoI r'AATT_y

Cuts at:   1044    1626    3975    4231    1044 
   Size:        582    2349     256    1435

  Fragments arranged by size: 

                2349    1435     582     256
 
AscI GG'CGCG_CC

Cuts at:   1595    1595 
   Size:       4622
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4622
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4622
 
AvaI C'yCGr_G

Cuts at:   1590    1642    1590 
   Size:         52    4570
 
BamHI G'GATC_C

Cuts at:   1647    1647 
   Size:       4622
 
BanI G'GyrC_C

Cuts at:    793    1547    1638    2052    2300    2335     793 
   Size:        754      91     414     248      35    3080

  Fragments arranged by size: 

                3080     754     414     248      91      35
 
BanII G_rGCy'C

Cuts at:    401    1636    2666     401 
   Size:       1235    1030    2357

  Fragments arranged by size: 

                2357    1235    1030
 
BbeI G_GCGC'C

Cuts at:   2056    2304    2056 
   Size:        248    4374
 
BbsI GAAGACnn'nnnn_

Cuts at:    325     366     413    1163    1211     325 
   Size:         41      47     750      48    3736

  Fragments arranged by size: 

                3736     750      48      47      41
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    424     458     424 
   Size:         34    4588
 
BciVI GTATCCnnnnn_n'

Cuts at:    466    2520    3576    4622     466 
   Size:       2054    1056    1046     466

  Fragments arranged by size: 

                2054    1056    1046     466
 
BclI T'GATC_A

Cuts at:   2142    2142 
   Size:       4622
 
BglI GCCn_nnn'nGGC

Cuts at:    901    1559     901 
   Size:        658    3964
 
BglII A'GATC_T

Cuts at:   2137    4174    2137 
   Size:       2037    2585
 
BlpI GC'TnA_GC

Cuts at:   1734    1734 
   Size:       4622
 
Bme1580I G_kGCm'C

Cuts at:   1442    1580    2247    2340    3464    1442 
   Size:        138     667      93    1124    2600

  Fragments arranged by size: 

                2600    1124     667     138      93
 
BmgBI CAC'GTC

Cuts at:   1443    1443 
   Size:       4622
 
BmrI ACTGGGnnnn_n'

Cuts at:   1345    1834    1915    2250    4394    1345 
   Size:        489      81     335    2144    1573

  Fragments arranged by size: 

                2144    1573     489     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    308    1036    2989     308 
   Size:        728    1953    1941

  Fragments arranged by size: 

                1953    1941     728
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    301     490    1186     301 
   Size:        189     696    3737

  Fragments arranged by size: 

                3737     696     189
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    502     991    1798    2677    3391    3689     502 
   Size:        489     807     879     714     298    1435

  Fragments arranged by size: 

                1435     879     807     714     489     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    506    1528    4326     506 
   Size:       1022    2798     802

  Fragments arranged by size: 

                2798    1022     802
 
BsaAI yAC'GTr

Cuts at:   1437    1975    1437 
   Size:        538    4084
 
BsaBI GATnn'nnATC

Cuts at:      6    2136    4173       6 
   Size:       2130    2037     455

  Fragments arranged by size: 

                2130    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2053    2301    3003    4499    2053 
   Size:        248     702    1496    2176

  Fragments arranged by size: 

                2176    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1811    2332    3010    3421    3568    3842    1811 
   Size:        521     678     411     147     274    2591

  Fragments arranged by size: 

                2591     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    331     361     816     846    1137    1167     331 
   Size:         30     455      30     291      30    3786

  Fragments arranged by size: 

                3786     455     291      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    367    1546     367 
   Size:       1179    3443
 
BseYI C'CCAG_C

Cuts at:   2047    2805    3470    3878    2047 
   Size:        758     665     408    2791

  Fragments arranged by size: 

                2791     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    111     195     619    1142     111 
   Size:         84     424     523    3591

  Fragments arranged by size: 

                3591     523     424      84
 
BsiEI CG_ry'CG

Cuts at:    104    2164    2210    3440     104 
   Size:       2060      46    1230    1286

  Fragments arranged by size: 

                2060    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    401    1442    1580    1636    2414    2604    3464     401 
   Size:       1041     138      56     778     190     860    1559

  Fragments arranged by size: 

                1559    1041     860     778     190     138      56
 
BsmI GAATG_Cn'

Cuts at:    315     315 
   Size:       4622
 
Bsp1286I G_dGCh'C

Cuts at:    401    1442    1580    1636    2247    2340    2414    2604    2666 
   Size:       1041     138      56     611      93      74     190      62

Cuts at:   2666    3464     401 
   Size:        798    1559

  Fragments arranged by size: 

                1559    1041     798     611     190     138      93      74
                  62      56
 
BspHI T'CATG_A

Cuts at:   3054    3054 
   Size:       4622
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    133     539    1394    1605    1672    1886    2188    2569     133 
   Size:        406     855     211      67     214     302     381    2186

  Fragments arranged by size: 

                2186     855     406     381     302     214     211      67
            
 
BsrBI CCG'CTC

Cuts at:     50    2914    2968    4452    4617      50 
   Size:       2864      54    1484     165      55

  Fragments arranged by size: 

                2864    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2534    2534 
   Size:       4622
 
BsrFI r'CCGG_y

Cuts at:    214    1508    2620    2801     214 
   Size:       1294    1112     181    2035

  Fragments arranged by size: 

                2035    1294    1112     181
 
BsrGI T'GTAC_A

Cuts at:    719     719 
   Size:       4622
 
BssHII G'CGCG_C

Cuts at:   1595    1595 
   Size:       4622
 
BssSI C'ACGA_G

Cuts at:    730    2893    3601     730 
   Size:       2163     708    1751

  Fragments arranged by size: 

                2163    1751     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1575    1999    1575 
   Size:        424    4198
 
BstBI TT'CG_AA

Cuts at:   2983    2983 
   Size:       4622
 
BstEII G'GTnAC_C

Cuts at:    120     120 
   Size:       4622
 
BstXI CCAn_nnnn'nTGG

Cuts at:    749    1032     749 
   Size:        283    4339
 
BstYI r'GATC_y

Cuts at:      1     296     464    1647    2114    2137    2472    2718    3024 
   Size:        295     168    1183     467      23     335     246     306

Cuts at:   3024    3036    3122    4174       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1183    1052     467     449     335     306     295     246
                 168      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    656     656 
   Size:       4622
 
BtgI C'CryG_G

Cuts at:    107     116     916     935    1241     107 
   Size:          9     800      19     306    3488

  Fragments arranged by size: 

                3488     800     306      19       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1452    2572    2753    2916    1452 
   Size:       1120     181     163    3158

  Fragments arranged by size: 

                3158    1120     181     163
 
BtsI GCAGTG_nn'

Cuts at:    569    1584    1865     569 
   Size:       1015     281    3326

  Fragments arranged by size: 

                3326    1015     281
 
DraI TTT'AAA

Cuts at:   1621    1621 
   Size:       4622
 
DrdI GACnn_nn'nnGTC

Cuts at:   2328    3672    2328 
   Size:       1344    3278
 
EaeI y'GGCC_r

Cuts at:    110     216     539    1034    1391    2207    2381    2772    2799 
   Size:        106     323     495     357     816     174     391      27

Cuts at:   2799    4144     110 
   Size:       1345     588

  Fragments arranged by size: 

                1345     816     588     495     391     357     323     174
                 106      27
 
EagI C'GGCC_G

Cuts at:   2207    2207 
   Size:       4622
 
EarI CTCTTCn'nnn_

Cuts at:   2645    2855    2645 
   Size:        210    4412
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2830    3572    3718    4432    2830 
   Size:        742     146     714    3020

  Fragments arranged by size: 

                3020     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    399    1634     399 
   Size:       1235    3387
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    301     331     490     857     928    1186    1349    2447    2879 
   Size:         30     159     367      71     258     163    1098     432

Cuts at:   2879    3232    3894     301 
   Size:        353     662    1029

  Fragments arranged by size: 

                1098    1029     662     432     367     353     258     163
                 159      71      30
 
EcoO109I rG'GnC_Cy

Cuts at:    660     903    1761     660 
   Size:        243     858    3521

  Fragments arranged by size: 

                3521     858     243
 
EcoRI G'AATT_C

Cuts at:   1626    1626 
   Size:       4622
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1165    2641    1165 
   Size:       1476    3146
 
FseI GG_CCGG'CC

Cuts at:   1512    1512 
   Size:       4622
 
FspI TGC'GCA

Cuts at:   2403    2403 
   Size:       4622
 
HaeII r_GCGC'y

Cuts at:   2056    2304    3534    4305    2056 
   Size:        248    1230     771    2373

  Fragments arranged by size: 

                2373    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    185     217     331     363     793     825    1137    1169    2464 
   Size:         32     114      32     430      32     312      32    1295

Cuts at:   2464    2494    2496    2526     185 
   Size:         30       2      30    2281

  Fragments arranged by size: 

                2281    1295     430     312     114      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   1266    1661    1266 
   Size:        395    4227
 
KasI G'GCGC_C

Cuts at:   2052    2300    2052 
   Size:        248    4374
 
KpnI G_GTAC'C

Cuts at:   1642    1642 
   Size:       4622
 
MluI A'CGCG_T

Cuts at:   4055    4055 
   Size:       4622
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    216     344    3381    3565     216 
   Size:        128    3037     184    1273

  Fragments arranged by size: 

                3037    1273     184     128
 
MscI TGG'CCA

Cuts at:    112     541    1036    1393    2383     112 
   Size:        429     495     357     990    2351

  Fragments arranged by size: 

                2351     990     495     429     357
 
MslI CAynn'nnrTG

Cuts at:    118     224    1027    2611    2738     118 
   Size:        106     803    1584     127    2002

  Fragments arranged by size: 

                2002    1584     803     127     106
 
MspA1I CmG'CkG

Cuts at:   1611    1733    2047    2407    3191    3436    1611 
   Size:        122     314     360     784     245    2797

  Fragments arranged by size: 

                2797     784     360     314     245     122
 
NaeI GCC'GGC

Cuts at:   1510    2803    1510 
   Size:       1293    3329
 
NarI GG'CG_CC

Cuts at:   2053    2301    2053 
   Size:        248    4374
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4622
 
NgoMIV G'CCGG_C

Cuts at:   1508    2801    1508 
   Size:       1293    3329
 
NspI r_CATG'y

Cuts at:    707    1675    2175    3778     707 
   Size:        968     500    1603    1551

  Fragments arranged by size: 

                1603    1551     968     500
 
PasI CC'CwG_GG

Cuts at:   1306    1306 
   Size:       4622
 
PciI A'CATG_T

Cuts at:   3774    3774 
   Size:       4622
 
PflMI CCAn_nnn'nTGG

Cuts at:    279     369     279 
   Size:         90    4532
 
PfoI T'CCnGG_A

Cuts at:    467     467 
   Size:       4622
 
PmeI GTTT'AAAC

Cuts at:   1621    1621 
   Size:       4622
 
PmlI CAC'GTG

Cuts at:   1437    1437 
   Size:       4622
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3068    3100    3068 
   Size:         32    4590
 
PpuMI rG'GwC_Cy

Cuts at:    660     660 
   Size:       4622
 
PstI C_TGCA'G

Cuts at:    564    1616    1669    2354     564 
   Size:       1052      53     685    2832

  Fragments arranged by size: 

                2832    1052     685      53
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4622
 
PvuII CAG'CTG

Cuts at:   2047    2407    2047 
   Size:        360    4262
 
RsrII CG'GwC_CG

Cuts at:   2817    2817 
   Size:       4622
 
SacI G_AGCT'C

Cuts at:    401    1636     401 
   Size:       1235    3387
 
SalI G'TCGA_C

Cuts at:   1659    1659 
   Size:       4622
 
SapI GCTCTTCn'nnn_

Cuts at:   2645    2855    2645 
   Size:        210    4412
 
SbfI CC_TGCA'GG

Cuts at:   1669    1669 
   Size:       4622
 
ScaI AGT'ACT

Cuts at:    427    4139     427 
   Size:       3712     910
 
SexAI A'CCwGG_T

Cuts at:    368    1343     368 
   Size:        975    3647
 
SfcI C'TryA_G

Cuts at:     33     429     560    1612    1665    2350    3318    3509      33 
   Size:        396     131    1052      53     685     968     191    1146

  Fragments arranged by size: 

                1146    1052     968     685     396     191     131      53
            
 
SfoI GGC'GCC

Cuts at:   2054    2302    2054 
   Size:        248    4374
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4622
 
SmaI CCC'GGG

Cuts at:   1644    1644 
   Size:       4622
 
SmlI C'TyrA_G

Cuts at:    517    1006    1777    2692    3406    3668     517 
   Size:        489     771     915     714     262    1471

  Fragments arranged by size: 

                1471     915     771     714     489     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4622
 
SphI G_CATG'C

Cuts at:   1675    2175    1675 
   Size:        500    4122
 
StuI AGG'CCT

Cuts at:   1285    1285 
   Size:       4622
 
StyI C'CwwG_G

Cuts at:    107    1756    2110     107 
   Size:       1649     354    2619

  Fragments arranged by size: 

                2619    1649     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2205    2338    2797    3006    2205 
   Size:        133     459     209    3821

  Fragments arranged by size: 

                3821     459     209     133
 
TatI w'GTAC_w

Cuts at:    425     719    1490    4137     425 
   Size:        294     771    2647     910

  Fragments arranged by size: 

                2647     910     771     294
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    816     848    4139    4171     816 
   Size:         32    3291      32    1267

  Fragments arranged by size: 

                3291    1267      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    826    2419     826 
   Size:       1593    3029
 
XbaI T'CTAG_A

Cuts at:   1653    1653 
   Size:       4622
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2117    2117 
   Size:       4622
 
XmaI C'CCGG_G

Cuts at:   1642    1642 
   Size:       4622
 
XmnI GAAnn'nnTTC

Cuts at:    985     985 
   Size:       4622
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AleI    AlwNI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FseI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AlfI     AloI     ApaI
    AvrII     BaeI    BbvCI    BfrBI     BmtI   Bpu10I    BsiWI    BsmBI
    BspEI  BstZ17I     ClaI    CspCI   DraIII    EcoNI    EcoRV    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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