Restriction map for pF1KB8435
 (Linear) (Six-base) MAPSORT of: KIBB8435.seq  Check: 491  from: 1  to: 1509



KIBB8435.orf

 With 182 enzymes: SgfI * 

                        August 7, 2008 14:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     749     820    1241    1509 
   Size:        223     526      71     421     268

  Fragments arranged by size: 

                 526     421     268     223      71
 
AflIII A'CryG_T

Cuts at:      0    1326    1509 
   Size:       1326     183
 
AhdI GACnn_n'nnGTC

Cuts at:      0      91     699    1509 
   Size:         91     608     810

  Fragments arranged by size: 

                 810     608      91
 
AleI CACnn'nnGTG

Cuts at:      0      10    1509 
   Size:         10    1499
 
AlwNI CAG_nnn'CTG

Cuts at:      0     171     344     360     756    1509 
   Size:        171     173      16     396     753

  Fragments arranged by size: 

                 753     396     173     171      16
 
ApaLI G'TGCA_C

Cuts at:      0    1330    1468    1509 
   Size:       1330     138      41

  Fragments arranged by size: 

                1330     138      41
 
ApoI r'AATT_y

Cuts at:      0     936    1509 
   Size:        936     573
 
AscI GG'CGCG_CC

Cuts at:      0    1487    1509 
   Size:       1487      22
 
AvaI C'yCGr_G

Cuts at:      0    1482    1509 
   Size:       1482      27
 
BanI G'GyrC_C

Cuts at:      0     685    1439    1509 
   Size:        685     754      70

  Fragments arranged by size: 

                 754     685      70
 
BanII G_rGCy'C

Cuts at:      0     293    1509 
   Size:        293    1216
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     217     258     305    1055    1103    1509 
   Size:        217      41      47     750      48     406

  Fragments arranged by size: 

                 750     406     217      48      47      41
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     316     350    1509 
   Size:        316      34    1159

  Fragments arranged by size: 

                1159     316      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     358    1509 
   Size:        358    1151
 
BglI GCCn_nnn'nGGC

Cuts at:      0     793    1451    1509 
   Size:        793     658      58

  Fragments arranged by size: 

                 793     658      58
 
Bme1580I G_kGCm'C

Cuts at:      0    1334    1472    1509 
   Size:       1334     138      37

  Fragments arranged by size: 

                1334     138      37
 
BmgBI CAC'GTC

Cuts at:      0    1335    1509 
   Size:       1335     174
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1237    1509 
   Size:       1237     272
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     200     928    1509 
   Size:        200     728     581

  Fragments arranged by size: 

                 728     581     200
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     382    1078    1509 
   Size:        193     189     696     431

  Fragments arranged by size: 

                 696     431     193     189
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     883    1509 
   Size:        394     489     626

  Fragments arranged by size: 

                 626     489     394
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     398    1420    1509 
   Size:        398    1022      89

  Fragments arranged by size: 

                1022     398      89
 
BsaAI yAC'GTr

Cuts at:      0    1329    1509 
   Size:       1329     180
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     223     253     708     738    1029    1059    1509 
   Size:        223      30     455      30     291      30     450

  Fragments arranged by size: 

                 455     450     291     223      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     259    1438    1509 
   Size:        259    1179      71

  Fragments arranged by size: 

                1179     259      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3      87     511    1034    1509 
   Size:          3      84     424     523     475

  Fragments arranged by size: 

                 523     475     424      84       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     293    1334    1472    1509 
   Size:        293    1041     138      37

  Fragments arranged by size: 

                1041     293     138      37
 
BsmI GAATG_Cn'

Cuts at:      0     207    1509 
   Size:        207    1302
 
Bsp1286I G_dGCh'C

Cuts at:      0     293    1334    1472    1509 
   Size:        293    1041     138      37

  Fragments arranged by size: 

                1041     293     138      37
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      25     431    1286    1509 
   Size:         25     406     855     223

  Fragments arranged by size: 

                 855     406     223      25
 
BsrFI r'CCGG_y

Cuts at:      0     106    1400    1509 
   Size:        106    1294     109

  Fragments arranged by size: 

                1294     109     106
 
BsrGI T'GTAC_A

Cuts at:      0     611    1509 
   Size:        611     898
 
BssHII G'CGCG_C

Cuts at:      0    1487    1509 
   Size:       1487      22
 
BssSI C'ACGA_G

Cuts at:      0     622    1509 
   Size:        622     887
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1467    1509 
   Size:       1467      42
 
BstEII G'GTnAC_C

Cuts at:      0      12    1509 
   Size:         12    1497
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     641     924    1509 
   Size:        641     283     585

  Fragments arranged by size: 

                 641     585     283
 
BstYI r'GATC_y

Cuts at:      0     188     356    1509 
   Size:        188     168    1153

  Fragments arranged by size: 

                1153     188     168
 
Bsu36I CC'TnA_GG

Cuts at:      0     548    1509 
   Size:        548     961
 
BtgI C'CryG_G

Cuts at:      0       8     808     827    1133    1509 
   Size:          8     800      19     306     376

  Fragments arranged by size: 

                 800     376     306      19       8
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1344    1509 
   Size:       1344     165
 
BtsI GCAGTG_nn'

Cuts at:      0     461    1476    1509 
   Size:        461    1015      33

  Fragments arranged by size: 

                1015     461      33
 
EaeI y'GGCC_r

Cuts at:      0       2     108     431     926    1283    1509 
   Size:          2     106     323     495     357     226

  Fragments arranged by size: 

                 495     357     323     226     106       2
 
EcoICRI GAG'CTC

Cuts at:      0     291    1509 
   Size:        291    1218
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     223     382     749     820    1078    1241    1509 
   Size:        193      30     159     367      71     258     163     268

  Fragments arranged by size: 

                 367     268     258     193     163     159      71      30
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     552     795    1509 
   Size:        552     243     714

  Fragments arranged by size: 

                 714     552     243
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1057    1509 
   Size:       1057     452
 
FseI GG_CCGG'CC

Cuts at:      0    1404    1509 
   Size:       1404     105
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      77     109     223     255     685     717    1029    1061 
   Size:         77      32     114      32     430      32     312      32

Cuts at:   1061    1509 
   Size:        448

  Fragments arranged by size: 

                 448     430     312     114      77      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1158    1509 
   Size:       1158     351
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     108     236    1509 
   Size:        108     128    1273

  Fragments arranged by size: 

                1273     128     108
 
MscI TGG'CCA

Cuts at:      0       4     433     928    1285    1509 
   Size:          4     429     495     357     224

  Fragments arranged by size: 

                 495     429     357     224       4
 
MslI CAynn'nnrTG

Cuts at:      0      10     116     919    1509 
   Size:         10     106     803     590

  Fragments arranged by size: 

                 803     590     106      10
 
MspA1I CmG'CkG

Cuts at:      0    1503    1509 
   Size:       1503       6
 
NaeI GCC'GGC

Cuts at:      0    1402    1509 
   Size:       1402     107
 
NgoMIV G'CCGG_C

Cuts at:      0    1400    1509 
   Size:       1400     109
 
NspI r_CATG'y

Cuts at:      0     599    1509 
   Size:        599     910
 
PasI CC'CwG_GG

Cuts at:      0    1198    1509 
   Size:       1198     311
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     171     261    1509 
   Size:        171      90    1248

  Fragments arranged by size: 

                1248     171      90
 
PfoI T'CCnGG_A

Cuts at:      0     359    1509 
   Size:        359    1150
 
PmlI CAC'GTG

Cuts at:      0    1329    1509 
   Size:       1329     180
 
PpuMI rG'GwC_Cy

Cuts at:      0     552    1509 
   Size:        552     957
 
PstI C_TGCA'G

Cuts at:      0     456    1508    1509 
   Size:        456    1052       1

  Fragments arranged by size: 

                1052     456       1
 
SacI G_AGCT'C

Cuts at:      0     293    1509 
   Size:        293    1216
 
ScaI AGT'ACT

Cuts at:      0     319    1509 
   Size:        319    1190
 
SexAI A'CCwGG_T

Cuts at:      0     260    1235    1509 
   Size:        260     975     274

  Fragments arranged by size: 

                 975     274     260
 
SfcI C'TryA_G

Cuts at:      0     321     452    1504    1509 
   Size:        321     131    1052       5

  Fragments arranged by size: 

                1052     321     131       5
 
SmlI C'TyrA_G

Cuts at:      0     409     898    1509 
   Size:        409     489     611

  Fragments arranged by size: 

                 611     489     409
 
StuI AGG'CCT

Cuts at:      0    1177    1509 
   Size:       1177     332
 
TatI w'GTAC_w

Cuts at:      0     317     611    1382    1509 
   Size:        317     294     771     127

  Fragments arranged by size: 

                 771     317     294     127
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     708     740    1509 
   Size:        708      32     769

  Fragments arranged by size: 

                 769     708      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     718    1509 
   Size:        718     791
 
XmnI GAAnn'nnTTC

Cuts at:      0     877    1509 
   Size:        877     632
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AleI    AlwNI    ApaLI     ApoI     AscI
     AvaI     BanI    BanII     BbsI     BcgI    BciVI     BglI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaXI
    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     EaeI  EcoICRI  Eco57MI EcoO109I     FalI     FseI
    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NaeI   NgoMIV
     NspI     PasI    PflMI     PfoI     PmlI    PpuMI     PstI     SacI
     ScaI    SexAI     SfcI     SmlI     StuI     TatI     TstI  Tth111I
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AlfI     AloI     ApaI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BclI    BfrBI    BglII     BlpI     BmtI   Bpu10I
    BsaBI    BsaHI    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BstBI  BstZ17I     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI    EcoNI    EcoRI    EcoRV
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI    TaqII     XbaI     XcmI
     XhoI     XmaI     ZraI
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