Restriction map for pF1KB7171
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfrI        BglI        BglII       BlpI        
Bpu1102I    BsaAI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BsrBI       BsrFI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRV       
FauI        FseI        FspI        HindIII     KasI        
KpnI        Ksp632I     KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp45I      Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcyI            3  GGCGTC               G[GA]^CG[CT]C
Aor51HI       895  AGCGCT               AGC^GCT
AspHI        1381  GAGCTC               G[AT]GC[AT]^C
AvaII         201  GGACC                G^G[AT]CC
AvrII         727  CCTAGG               C^CTAGG
BclI          657  TGATCA               T^GATCA
BcnI          480  CCGGG                CC^[GC]GG
BlnI          727  CCTAGG               C^CTAGG
BsaBI         973  GATGACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
BsiHKAI      1381  GAGCTC               G[AT]GC[AT]^C
BspHI        1052  TCATGA               T^CATGA
BspLU11I      314  ACATGT               A^CATGT
BssSI         997  CTCGTG               CACGAG(-5/-1)
CfoI          896  GCGC                 GCG^C
EaeI         1133  TGGCCG               [CT]^GGCC[GA]
Eco47III      895  AGCGCT               AGC^GCT
EcoNI         266  CCTACGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1355  GAATTC               G^AATTC
EcoT22I      1257  ATGCAT               ATGCA^T
FbaI          657  TGATCA               T^GATCA
HaeII         895  AGCGCT               [GA]GCGC^[CT]
HapII         480  CCGG                 C^CGG
HhaI          896  GCGC                 GCG^C
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
HinP1I        896  GCGC                 G^CGC
HincII        744  GTTAAC               GT[CT]^[GA]AC
HindII        744  GTTAAC               GT[CT]^[GA]AC
HpaI          744  GTTAAC               GTT^AAC
HpaII         480  CCGG                 C^CGG
MaeII        1389  ACGT                 A^CGT
MaeIII       1064  GTTAC                ^GT[ACGT]AC
MamI          973  GATGACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        157  CAGCGG               C[AC]G^C[GT]G
MspI          480  CCGG                 C^CGG
NciI          480  CCGGG                CC^[GC]GG
NsiI         1257  ATGCAT               ATGCA^T
NspI          314  ACATGT               [GA]CATG^[CT]
PflMI         115  CCAGGCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          713  CTGCAG               CTGCA^G
RcaI         1052  TCATGA               T^CATGA
SacI         1381  GAGCTC               GAGCT^C
TaqI         1128  TCGA                 T^CGA
TthHB8I      1128  TCGA                 T^CGA
Van91I        115  CCAGGCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          504  CCATAATAGATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI          523  AAATTT               [GA]^AATT[CT]
             1355  GAATTC              
AfaI          477  GTAC                 GT^AC
             1436  GTAC                
AflIII        314  ACATGT               A^C[GA][CT]GT
             1387  ACACGT              
ApoI          523  AAATTT               [GA]^AATT[CT]
             1355  GAATTC              
BanII          58  GAGCCC               G[GA]GC[CT]^C
             1381  GAGCTC              
BmyI           58  GAGCCC               G[AGT]GC[ACT]^C
             1381  GAGCTC              
BseRI         205  CTCCTC               GAGGAG(10/8)
             1378  GAGGAG              
BsmI         1085  GAATGC               GAATGC(1/-1)
              461  GCATTC              
Bsp1286I       58  GAGCCC               G[AGT]GC[ACT]^C
             1381  GAGCTC              
BspMI         780  ACCTGC               ACCTGC(4/8)
              272  GCAGGT              
BstYI         567  AGATCC               [GA]^GATC[CT]
              633  AGATCC              
DraI          775  TTTAAA               TTT^AAA
              879  TTTAAA              
HgaI            4  GCGTC                GACGC(5/10)
             1270  GCGTC               
HphI          191  TCACC                GGTGA(8/7)
              796  TCACC               
MflI          567  AGATCC               [GA]^GATC[CT]
              633  AGATCC              
NcoI           49  CCATGG               C^CATGG
               77  CCATGG              
RsaI          477  GTAC                 GT^AC
             1436  GTAC                
SfaNI         153  GATGC                GCATC(5/9)
              859  GATGC               
SfcI          494  CTACAG               C^T[GA][CT]AG
              713  CTGCAG              
TfiI          393  GAATC                G^A[AT]TC
              718  GATTC               
XhoII         567  AGATCC               [GA]^GATC[CT]
              633  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AluI        AlwI        BbsI        BbvI        
BfaI        BpmI        BpuAI       BsaI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       Cfr13I      DdeI        DpnII       DsaI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HinfI       ItaI        MaeI        MboI        MboII       
MnlI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       StyI        Tru9I       TseI        
Tsp509I     TspRI       
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