Restriction map for pF1KB8907
 (Linear) (Six-base) MAPSORT of: KIBB8907.seq  Check: 30  from: 1  to: 717



KIBB8907.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     205     717 
   Size:         92     113     512

  Fragments arranged by size: 

                 512     113      92
 
AlwNI CAG_nnn'CTG

Cuts at:      0     237     717 
   Size:        237     480
 
ApaI G_GGCC'C

Cuts at:      0     481     717 
   Size:        481     236
 
BanI G'GyrC_C

Cuts at:      0     121     717 
   Size:        121     596
 
BanII G_rGCy'C

Cuts at:      0     355     481     492     717 
   Size:        355     126      11     225

  Fragments arranged by size: 

                 355     225     126      11
 
BbeI G_GCGC'C

Cuts at:      0     125     717 
   Size:        125     592
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      70     717 
   Size:         70     647
 
BglI GCCn_nnn'nGGC

Cuts at:      0     344     717 
   Size:        344     373
 
Bme1580I G_kGCm'C

Cuts at:      0     481     717 
   Size:        481     236
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     519     717 
   Size:        519     198
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     348     603     717 
   Size:        348     255     114

  Fragments arranged by size: 

                 348     255     114
 
BsaHI Gr'CG_yC

Cuts at:      0      25     122     142     717 
   Size:         25      97      20     575

  Fragments arranged by size: 

                 575      97      25      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     298     717 
   Size:        298     419
 
BseYI C'CCAG_C

Cuts at:      0     108     377     457     660     717 
   Size:        108     269      80     203      57

  Fragments arranged by size: 

                 269     203     108      80      57
 
BsiEI CG_ry'CG

Cuts at:      0     411     717 
   Size:        411     306
 
Bsp1286I G_dGCh'C

Cuts at:      0     355     481     492     717 
   Size:        355     126      11     225

  Fragments arranged by size: 

                 355     225     126      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      41     212     717 
   Size:         41     171     505

  Fragments arranged by size: 

                 505     171      41
 
BsrBI CCG'CTC

Cuts at:      0     288     362     717 
   Size:        288      74     355

  Fragments arranged by size: 

                 355     288      74
 
BsrDI GCAATG_nn'

Cuts at:      0     306     717 
   Size:        306     411
 
BsrFI r'CCGG_y

Cuts at:      0     151     410     717 
   Size:        151     259     307

  Fragments arranged by size: 

                 307     259     151
 
BstEII G'GTnAC_C

Cuts at:      0     240     717 
   Size:        240     477
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     465     717 
   Size:        465     252
 
BstYI r'GATC_y

Cuts at:      0     224     717 
   Size:        224     493
 
BtgI C'CryG_G

Cuts at:      0      11      84     717 
   Size:         11      73     633

  Fragments arranged by size: 

                 633      73      11
 
BtsI GCAGTG_nn'

Cuts at:      0     272     697     717 
   Size:        272     425      20

  Fragments arranged by size: 

                 425     272      20
 
ClaI AT'CG_AT

Cuts at:      0     215     717 
   Size:        215     502
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      15     717 
   Size:         15     702
 
EaeI y'GGCC_r

Cuts at:      0     153     717 
   Size:        153     564
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     205     717 
   Size:         92     113     512

  Fragments arranged by size: 

                 512     113      92
 
EcoO109I rG'GnC_Cy

Cuts at:      0     477     717 
   Size:        477     240
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     681     717 
   Size:        681      36
 
HaeII r_GCGC'y

Cuts at:      0     125     184     717 
   Size:        125      59     533

  Fragments arranged by size: 

                 533     125      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     409     441     717 
   Size:        409      32     276

  Fragments arranged by size: 

                 409     276      32
 
HincII GTy'rAC

Cuts at:      0     299     717 
   Size:        299     418
 
KasI G'GCGC_C

Cuts at:      0     121     717 
   Size:        121     596
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     717 
   Size:        260     457
 
MslI CAynn'nnrTG

Cuts at:      0     217     717 
   Size:        217     500
 
MspA1I CmG'CkG

Cuts at:      0      86     237     377     717 
   Size:         86     151     140     340

  Fragments arranged by size: 

                 340     151     140      86
 
NarI GG'CG_CC

Cuts at:      0     122     717 
   Size:        122     595
 
PasI CC'CwG_GG

Cuts at:      0     136     604     717 
   Size:        136     468     113

  Fragments arranged by size: 

                 468     136     113
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     162     717 
   Size:        162     555
 
PfoI T'CCnGG_A

Cuts at:      0     440     717 
   Size:        440     277
 
PspOMI G'GGCC_C

Cuts at:      0     477     717 
   Size:        477     240
 
PstI C_TGCA'G

Cuts at:      0     233     717 
   Size:        233     484
 
PvuII CAG'CTG

Cuts at:      0     237     377     717 
   Size:        237     140     340

  Fragments arranged by size: 

                 340     237     140
 
SacII CC_GC'GG

Cuts at:      0      87     717 
   Size:         87     630
 
SfcI C'TryA_G

Cuts at:      0     229     717 
   Size:        229     488
 
SfoI GGC'GCC

Cuts at:      0     123     717 
   Size:        123     594
 
SmlI C'TyrA_G

Cuts at:      0     363     582     717 
   Size:        363     219     135

  Fragments arranged by size: 

                 363     219     135
 
Tth111I GACn'n_nGTC

Cuts at:      0     421     717 
   Size:        421     296
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     411     549     717 
   Size:        411     138     168

  Fragments arranged by size: 

                 411     168     138
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     BanI    BanII     BbeI     BbsI     BglI
 Bme1580I     BmrI    BpuEI    BsaHI    BseRI    BseYI    BsiEI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI   BstEII    BstXI    BstYI     BtgI
     BtsI     ClaI     DrdI     EaeI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I   HincII     KasI     MmeI     MslI   MspA1I     NarI     PasI
    PflMI     PfoI   PspOMI     PstI    PvuII    SacII     SfcI     SfoI
     SmlI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrGI   BssHII
    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI    CspCI     DraI
   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI     XbaI     XhoI     XmaI     XmnI
     ZraI
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