Restriction map for pF1KB6384
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstXI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MslI        MspA1I      MunI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          475  GTCTAC               GT^[AC][GT]AC
AccII          74  CGCG                 CG^CG
AlwI          739  GATCC                GGATC(4/5)
AocI          827  CCTCAGG              CC^T[ACGT]AGG
ApaI          823  GGGCCC               GGGCC^C
AspHI         253  GAGCAC               G[AT]GC[AT]^C
AvaI           21  CCCGGG               C^[CT]CG[GA]G
BalI          813  TGGCCA               TGG^CCA
BanII         823  GGGCCC               G[GA]GC[CT]^C
BclI          390  TGATCA               T^GATCA
BglI          606  GCCCCAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         515  AGATCT               A^GATCT
BsaBI         771  GATCGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          712  GAGACC               GGTCTC(1/5)
BsaWI         838  TCCGGT               [AT]^CCGG[AT]
BsgI          799  GTGCAG               GTGCAG(16/14)
BsiHKAI       253  GAGCAC               G[AT]GC[AT]^C
BsmAI         712  GAGAC                GTCTC(1/5)
BsoBI          21  CCCGGG               C^[CT]CG[GA]G
BstEII        108  GGTGACC              G^GT[ACGT]ACC
BstPI         108  GGTGACC              G^GT[ACGT]ACC
BstUI          74  CGCG                 CG^CG
Bsu36I        827  CCTCAGG              CC^T[ACGT]AGG
CfoI           75  GCGC                 GCG^C
DrdI          833  GACCCTCCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          813  TGGCCA               [CT]^GGCC[GA]
EarI          709  GAAGAG               CTCTTC(1/4)
Eco81I        827  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       108  GGTGACC              G^GT[ACGT]ACC
FbaI          390  TGATCA               T^GATCA
HhaI           75  GCGC                 GCG^C
HinP1I         75  GCGC                 G^CGC
Ksp632I       709  GAAGAG               CTCTTC(1/4)
MaeII         138  ACGT                 A^CGT
MamI          771  GATCGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         813  TGGCCA               TGG^CCA
MscI          813  TGGCCA               TGG^CCA
MvnI           74  CGCG                 CG^CG
NdeI          142  CATATG               CA^TATG
NspI          668  GCATGT               [GA]CATG^[CT]
PflMI         653  CCAAGCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         831  AGGACCC              [GA]G^G[AT]CC[CT]
SbfI          805  CCTGCAGG             CCTGCA^GG
SmaI           21  CCCGGG               CCC^GGG
Sse8387I      805  CCTGCAGG             CCTGCA^GG
Tsp509I       282  AATT                 ^AATT
Van91I        653  CCAAGCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          21  CCCGGG               C^CCGGG
XmaI           21  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI           43  CCGC                 CCGC(-3/-1)
              579  CCGC                
AvaII         832  GGACC                G^G[AT]CC
              841  GGTCC               
BbsI          505  GAAGAC               GAAGAC(2/6)
              318  GTCTTC              
BfaI           82  CTAG                 C^TAG
              736  CTAG                
BmyI          253  GAGCAC               G[AGT]GC[ACT]^C
              823  GGGCCC              
BpmI           49  CTGGAG               CTGGAG(16/14)
              361  CTGGAG              
BpuAI         505  GAAGAC               GAAGAC(2/6)
              318  GTCTTC              
BsmFI          18  GTCCC                GGGAC(10/14)
              842  GTCCC               
Bsp1286I      253  GAGCAC               G[AGT]GC[ACT]^C
              823  GGGCCC              
BspMI         698  ACCTGC               ACCTGC(4/8)
              808  GCAGGT              
BsrDI         449  GCAATG               GCAATG(2/0)
              657  GCAATG              
BstF5I         78  CATCC                GGATG(2/0)
              249  GGATG               
BstYI         515  AGATCT               [GA]^GATC[CT]
              738  AGATCC              
FokI           78  CATCC                GGATG(9/13)
              249  GGATG               
MaeI           82  CTAG                 C^TAG
              736  CTAG                
MaeIII        100  GTGAC                ^GT[ACGT]AC
              109  GTGAC               
MflI          515  AGATCT               [GA]^GATC[CT]
              738  AGATCC              
MseI          284  TTAA                 T^TAA
              755  TTAA                
PstI           33  CTGCAG               CTGCA^G
              806  CTGCAG              
SfcI           33  CTGCAG               C^T[GA][CT]AG
              806  CTGCAG              
TfiI           85  GAATC                G^A[AT]TC
              442  GATTC               
Tru9I         284  TTAA                 T^TAA
              755  TTAA                
Tsp45I        100  GTGAC                ^GT[GC]AC
              109  GTGAC               
XhoII         515  AGATCT               [GA]^GATC[CT]
              738  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AluI        BbvI        BcnI        BsaJI       BseRI       
BsiYI       BslI        BsrI        BssKI       BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      Fnu4HI      HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MboI        
MboII       MnlI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
TspRI       
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