Restriction map for pF1KB6384
 (Circular) (Six-base) MAPSORT of: pF1KB6384.seq  Check: 7419  from: 1  to: 4013



pF1KB6384  4013 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    907     907 
   Size:       4013
 
AccI GT'mk_AC

Cuts at:    584    1051     584 
   Size:        467    3546
 
Acc65I G'GTAC_C

Cuts at:   1029    1029 
   Size:       4013
 
AclI AA'CG_TT

Cuts at:   3853    3853 
   Size:       4013
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    314    1838    2270    3017    3285     314 
   Size:       1524     432     747     268    1042

  Fragments arranged by size: 

                1524    1042     747     432     268
 
AflIII A'CryG_T

Cuts at:   1365    2469    3446    1365 
   Size:       1104     977    1932

  Fragments arranged by size: 

                1932    1104     977
 
AgeI A'CCGG_T

Cuts at:   2401    2401 
   Size:       4013
 
AlwNI CAG_nnn'CTG

Cuts at:   1318    2885    1318 
   Size:       1567    2446
 
ApaI G_GGCC'C

Cuts at:    935     935 
   Size:       4013
 
ApaLI G'TGCA_C

Cuts at:   2783    2783 
   Size:       4013
 
ApoI r'AATT_y

Cuts at:   1017    3366    3622    1017 
   Size:       2349     256    1408

  Fragments arranged by size: 

                2349    1408     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4013
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4013
 
AvaI C'yCGr_G

Cuts at:    129    1033     129 
   Size:        904    3109
 
BamHI G'GATC_C

Cuts at:   1038    1038 
   Size:       4013
 
BanI G'GyrC_C

Cuts at:   1029    1443    1691    1726    1029 
   Size:        414     248      35    3316

  Fragments arranged by size: 

                3316     414     248      35
 
BanII G_rGCy'C

Cuts at:    935    1027    2057     935 
   Size:         92    1030    2891

  Fragments arranged by size: 

                2891    1030      92
 
BbeI G_GCGC'C

Cuts at:   1447    1695    1447 
   Size:        248    3765
 
BbsI GAAGACnn'nnnn_

Cuts at:    419     620     419 
   Size:        201    3812
 
BciVI GTATCCnnnnn_n'

Cuts at:   1911    2672    4013    1911 
   Size:        761    1341    1911

  Fragments arranged by size: 

                1911    1341     761
 
BclI T'GATC_A

Cuts at:    498    1533     498 
   Size:       1035    2978
 
BglI GCCn_nnn'nGGC

Cuts at:    720     720 
   Size:       4013
 
BglII A'GATC_T

Cuts at:    623    1528    3565     623 
   Size:        905    2037    1071

  Fragments arranged by size: 

                2037    1071     905
 
BlpI GC'TnA_GC

Cuts at:   1125    1125 
   Size:       4013
 
Bme1580I G_kGCm'C

Cuts at:    935    1638    1731    2787     935 
   Size:        703      93    1056    2161

  Fragments arranged by size: 

                2161    1056     703      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    385     417     711    1225    1306    1641    3785     385 
   Size:         32     294     514      81     335    2144     613

  Fragments arranged by size: 

                2144     613     514     335     294      81      32
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    843    2380     843 
   Size:       1537    2476
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    178     490     178 
   Size:        312    3701
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1189    2068    2560    2858    1189 
   Size:        879     492     298    2344

  Fragments arranged by size: 

                2344     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    814    3717     814 
   Size:       2903    1110
 
BsaAI yAC'GTr

Cuts at:   1366    1366 
   Size:       4013
 
BsaBI GATnn'nnATC

Cuts at:      6     883    1527    3564       6 
   Size:        877     644    2037     455

  Fragments arranged by size: 

                2037     877     644     455
 
BsaHI Gr'CG_yC

Cuts at:   1444    1692    2394    3890    1444 
   Size:        248     702    1496    1567

  Fragments arranged by size: 

                1567    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    946    1202    1723    2401    2675    2822    3233     946 
   Size:        256     521     678     274     147     411    1726

  Fragments arranged by size: 

                1726     678     521     411     274     256     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    109     139     109 
   Size:         30    3983
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    127     130     969     127 
   Size:          3     839    3171

  Fragments arranged by size: 

                3171     839       3
 
BseYI C'CCAG_C

Cuts at:    135     850    1438    2196    2773    3269     135 
   Size:        715     588     758     577     496     879

  Fragments arranged by size: 

                 879     758     715     588     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    928     928 
   Size:       4013
 
BsiEI CG_ry'CG

Cuts at:    104    1555    1601    2809     104 
   Size:       1451      46    1208    1308

  Fragments arranged by size: 

                1451    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    365    1027    1805    1995    2787     365 
   Size:        662     778     190     792    1591

  Fragments arranged by size: 

                1591     792     778     662     190
 
Bsp1286I G_dGCh'C

Cuts at:    365     935    1027    1638    1731    1805    1995    2057    2787 
   Size:        570      92     611      93      74     190      62     730

Cuts at:   2787     365 
   Size:       1591

  Fragments arranged by size: 

                1591     730     611     570     190      93      92      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3233    3233 
   Size:       4013
 
BspHI T'CATG_A

Cuts at:   3189    3189 
   Size:       4013
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    815     907    1063    1277    1579    1960     815 
   Size:         92     156     214     302     381    2868

  Fragments arranged by size: 

                2868     381     302     214     156      92
 
BsrBI CCG'CTC

Cuts at:     50    2305    2359    3843    4008      50 
   Size:       2255      54    1484     165      55

  Fragments arranged by size: 

                2255    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    564     772    1925     564 
   Size:        208    1153    2652

  Fragments arranged by size: 

                2652    1153     208
 
BsrFI r'CCGG_y

Cuts at:   2011    2192    2401    2011 
   Size:        181     209    3623

  Fragments arranged by size: 

                3623     209     181
 
BssSI C'ACGA_G

Cuts at:   2284    2642    2284 
   Size:        358    3655
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1390    1390 
   Size:       4013
 
BstBI TT'CG_AA

Cuts at:   2374    2374 
   Size:       4013
 
BstEII G'GTnAC_C

Cuts at:    216     216 
   Size:       4013
 
BstYI r'GATC_y

Cuts at:      1     623     846    1038    1505    1528    1863    2109    3121 
   Size:        622     223     192     467      23     335     246    1012

Cuts at:   3121    3207    3219    3565       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     622     467     449     346     335     246     223
                 192      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    936     936 
   Size:       4013
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4013
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1963    2144    2307    1963 
   Size:        181     163    3669

  Fragments arranged by size: 

                3669     181     163
 
BtsI GCAGTG_nn'

Cuts at:    537     570    1256     537 
   Size:         33     686    3294

  Fragments arranged by size: 

                3294     686      33
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    546     581     546 
   Size:         35    3978
 
DraI TTT'AAA

Cuts at:   1012    1012 
   Size:       4013
 
DrdI GACnn_nn'nnGTC

Cuts at:    947    1719    2577     947 
   Size:        772     858    2383

  Fragments arranged by size: 

                2383     858     772
 
EaeI y'GGCC_r

Cuts at:    921    1598    1772    2163    2190    3535     921 
   Size:        677     174     391      27    1345    1399

  Fragments arranged by size: 

                1399    1345     677     391     174      27
 
EagI C'GGCC_G

Cuts at:   1598    1598 
   Size:       4013
 
EarI CTCTTCn'nnn_

Cuts at:    812    2036    2246     812 
   Size:       1224     210    2579

  Fragments arranged by size: 

                2579    1224     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2221    2531    2677    3823    2221 
   Size:        310     146    1146    2411

  Fragments arranged by size: 

                2411    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1025    1025 
   Size:       4013
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    178     314     490    1838    2270    3017    3285     178 
   Size:        136     176    1348     432     747     268     906

  Fragments arranged by size: 

                1348     906     747     432     268     176     136
 
EcoO109I rG'GnC_Cy

Cuts at:    901     931     932     940    1152     901 
   Size:         30       1       8     212    3762

  Fragments arranged by size: 

                3762     212      30       8       1
 
EcoRI G'AATT_C

Cuts at:   1017    1017 
   Size:       4013
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    534     595    2032     534 
   Size:         61    1437    2515

  Fragments arranged by size: 

                2515    1437      61
 
FspI TGC'GCA

Cuts at:   1794    1794 
   Size:       4013
 
HaeII r_GCGC'y

Cuts at:   1447    1695    2717    3696    1447 
   Size:        248    1022     979    1764

  Fragments arranged by size: 

                1764    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    106     109     138     141    1855    1885    1887    1917     106 
   Size:          3      29       3    1714      30       2      30    2202

  Fragments arranged by size: 

                2202    1714      30      30      29       3       3       2
            
 
HincII GTy'rAC

Cuts at:   1052    1052 
   Size:       4013
 
KasI G'GCGC_C

Cuts at:   1443    1691    1443 
   Size:        248    3765
 
KpnI G_GTAC'C

Cuts at:   1033    1033 
   Size:       4013
 
MluI A'CGCG_T

Cuts at:   3446    3446 
   Size:       4013
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2684    2868    2684 
   Size:        184    3829
 
MscI TGG'CCA

Cuts at:    923    1774     923 
   Size:        851    3162
 
MslI CAynn'nnrTG

Cuts at:   2002    2129    2002 
   Size:        127    3886
 
MspA1I CmG'CkG

Cuts at:   1124    1438    1798    2811    3056    1124 
   Size:        314     360    1013     245    2081

  Fragments arranged by size: 

                2081    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2194    2194 
   Size:       4013
 
NarI GG'CG_CC

Cuts at:   1444    1692    1444 
   Size:        248    3765
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4013
 
NdeI CA'TA_TG

Cuts at:    251     251 
   Size:       4013
 
NgoMIV G'CCGG_C

Cuts at:   2192    2192 
   Size:       4013
 
NspI r_CATG'y

Cuts at:    780    1066    1566    2473     780 
   Size:        286     500     907    2320

  Fragments arranged by size: 

                2320     907     500     286
 
PasI CC'CwG_GG

Cuts at:    171     171 
   Size:       4013
 
PciI A'CATG_T

Cuts at:   2469    2469 
   Size:       4013
 
PflMI CCAn_nnn'nTGG

Cuts at:    767     767 
   Size:       4013
 
PfoI T'CCnGG_A

Cuts at:    632     632 
   Size:       4013
 
PmeI GTTT'AAAC

Cuts at:   1012    1012 
   Size:       4013
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    613     645    3152    3184     613 
   Size:         32    2507      32    1442

  Fragments arranged by size: 

                2507    1442      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    940     940 
   Size:       4013
 
PspOMI G'GGCC_C

Cuts at:    931     931 
   Size:       4013
 
PstI C_TGCA'G

Cuts at:    145     918    1060    1745     145 
   Size:        773     142     685    2413

  Fragments arranged by size: 

                2413     773     685     142
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4013
 
PvuII CAG'CTG

Cuts at:   1438    1798    1438 
   Size:        360    3653
 
RsrII CG'GwC_CG

Cuts at:   2208    2208 
   Size:       4013
 
SacI G_AGCT'C

Cuts at:   1027    1027 
   Size:       4013
 
SalI G'TCGA_C

Cuts at:   1050    1050 
   Size:       4013
 
SapI GCTCTTCn'nnn_

Cuts at:   2036    2246    2036 
   Size:        210    3803
 
SbfI CC_TGCA'GG

Cuts at:    918    1060     918 
   Size:        142    3871
 
ScaI AGT'ACT

Cuts at:   3530    3530 
   Size:       4013
 
SfcI C'TryA_G

Cuts at:     33     141     914    1056    1741    2734    2925      33 
   Size:        108     773     142     685     993     191    1121

  Fragments arranged by size: 

                1121     993     773     685     191     142     108
 
SfoI GGC'GCC

Cuts at:   1445    1693    1445 
   Size:        248    3765
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4013
 
SmaI CCC'GGG

Cuts at:    131    1035     131 
   Size:        904    3109
 
SmlI C'TyrA_G

Cuts at:   1168    2083    2575    2837    1168 
   Size:        915     492     262    2344

  Fragments arranged by size: 

                2344     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4013
 
SphI G_CATG'C

Cuts at:   1066    1566    1066 
   Size:        500    3513
 
StyI C'CwwG_G

Cuts at:    107    1147    1501     107 
   Size:       1040     354    2619

  Fragments arranged by size: 

                2619    1040     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    436     468     715     838    1596    1729    2188    2397     436 
   Size:         32     247     123     758     133     459     209    2052

  Fragments arranged by size: 

                2052     758     459     247     209     133     123      32
            
 
TatI w'GTAC_w

Cuts at:   3528    3528 
   Size:       4013
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3530    3562    3530 
   Size:         32    3981
 
Tth111I GACn'n_nGTC

Cuts at:   1810    1810 
   Size:       4013
 
XbaI T'CTAG_A

Cuts at:   1044    1044 
   Size:       4013
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1508    1508 
   Size:       4013
 
XmaI C'CCGG_G

Cuts at:    129    1033     129 
   Size:        904    3109
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI   Bpu10I
    BsiWI     BsmI    BsmBI    BsrGI   BssHII    BstXI  BstZ17I     ClaI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NheI     NotI     NruI     NsiI     PacI     PmlI    PshAI     PsiI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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