Restriction map for
pF1KB6384
(Linear) (Six-base) MAPSORT of: KIBB6384.seq Check: 8074 from: 1 to: 900
KIBB6384.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 799 900
Size: 799 101
AccI GT'mk_AC
Cuts at: 0 476 900
Size: 476 424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 900
Size: 206 694
ApaI G_GGCC'C
Cuts at: 0 827 900
Size: 827 73
AvaI C'yCGr_G
Cuts at: 0 21 900
Size: 21 879
BanII G_rGCy'C
Cuts at: 0 827 900
Size: 827 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 311 512 900
Size: 311 201 388
Fragments arranged by size:
388 311 201
BclI T'GATC_A
Cuts at: 0 390 900
Size: 390 510
BglI GCCn_nnn'nGGC
Cuts at: 0 612 900
Size: 612 288
BglII A'GATC_T
Cuts at: 0 515 900
Size: 515 385
Bme1580I G_kGCm'C
Cuts at: 0 827 900
Size: 827 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 277 309 603 900
Size: 277 32 294 297
Fragments arranged by size:
297 294 277 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 735 900
Size: 735 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 382 900
Size: 70 312 518
Fragments arranged by size:
518 312 70
BsaI GGTCTCn'nnnn_
Cuts at: 0 706 900
Size: 706 194
BsaBI GATnn'nnATC
Cuts at: 0 775 900
Size: 775 125
BsaWI w'CCGG_w
Cuts at: 0 838 900
Size: 838 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 900
Size: 1 30 869
Fragments arranged by size:
869 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 22 861 900
Size: 19 3 839 39
Fragments arranged by size:
839 39 19 3
BseYI C'CCAG_C
Cuts at: 0 27 742 900
Size: 27 715 158
Fragments arranged by size:
715 158 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 900
Size: 820 80
BsiHKAI G_wGCw'C
Cuts at: 0 257 900
Size: 257 643
Bsp1286I G_dGCh'C
Cuts at: 0 257 827 900
Size: 257 570 73
Fragments arranged by size:
570 257 73
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 707 799 900
Size: 707 92 101
Fragments arranged by size:
707 101 92
BsrDI GCAATG_nn'
Cuts at: 0 456 664 900
Size: 456 208 236
Fragments arranged by size:
456 236 208
BstEII G'GTnAC_C
Cuts at: 0 108 900
Size: 108 792
BstYI r'GATC_y
Cuts at: 0 515 738 900
Size: 515 223 162
Fragments arranged by size:
515 223 162
Bsu36I CC'TnA_GG
Cuts at: 0 828 900
Size: 828 72
BtsI GCAGTG_nn'
Cuts at: 0 429 462 900
Size: 429 33 438
Fragments arranged by size:
438 429 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 438 473 900
Size: 438 35 427
Fragments arranged by size:
438 427 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 839 900
Size: 839 61
EaeI y'GGCC_r
Cuts at: 0 813 900
Size: 813 87
EarI CTCTTCn'nnn_
Cuts at: 0 704 900
Size: 704 196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 206 382 900
Size: 70 136 176 518
Fragments arranged by size:
518 176 136 70
EcoO109I rG'GnC_Cy
Cuts at: 0 793 823 824 832 900
Size: 793 30 1 8 68
Fragments arranged by size:
793 68 30 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 426 487 900
Size: 426 61 413
Fragments arranged by size:
426 413 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 30 33 900
Size: 1 29 3 867
Fragments arranged by size:
867 29 3 1
MscI TGG'CCA
Cuts at: 0 815 900
Size: 815 85
NdeI CA'TA_TG
Cuts at: 0 143 900
Size: 143 757
NspI r_CATG'y
Cuts at: 0 672 900
Size: 672 228
PasI CC'CwG_GG
Cuts at: 0 63 900
Size: 63 837
PflMI CCAn_nnn'nTGG
Cuts at: 0 659 900
Size: 659 241
PfoI T'CCnGG_A
Cuts at: 0 524 900
Size: 524 376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 900
Size: 505 32 363
Fragments arranged by size:
505 363 32
PpuMI rG'GwC_Cy
Cuts at: 0 832 900
Size: 832 68
PspOMI G'GGCC_C
Cuts at: 0 823 900
Size: 823 77
PstI C_TGCA'G
Cuts at: 0 37 810 900
Size: 37 773 90
Fragments arranged by size:
773 90 37
SbfI CC_TGCA'GG
Cuts at: 0 810 900
Size: 810 90
SfcI C'TryA_G
Cuts at: 0 33 806 900
Size: 33 773 94
Fragments arranged by size:
773 94 33
SmaI CCC'GGG
Cuts at: 0 23 900
Size: 23 877
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 328 360 607 730 900
Size: 328 32 247 123 170
Fragments arranged by size:
328 247 170 123 32
XmaI C'CCGG_G
Cuts at: 0 21 900
Size: 21 879
Enzymes that do cut:
AarI AccI AcuI ApaI AvaI BanII BbsI BclI
BglI BglII Bme1580I BmrI BplI BpmI BsaI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BstEII BstYI Bsu36I BtsI CspCI DrdI EaeI
EarI Eco57MI EcoO109I FalI Hin4I MscI NdeI NspI
PasI PflMI PfoI PpiI PpuMI PspOMI PstI SbfI
SfcI SmaI TaqII XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BlpI BmgBI BmtI Bpu10I BpuEI BsaAI
BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
BtgI BtgZI ClaI DraI DraIII EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI