Restriction map for pF1KE0424
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       AvrII       BbeI        BbrPI       BbsI        
BciVI       BclI        BfrI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      ClaI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HindIII     HpaI        KasI        
MamI        MfeI        MluI        MroI        MseI        
MunI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
SacI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        952  GGTACC               G^GTACC
AccI          406  GTCGAC               GT^[AC][GT]AC
AccII          41  CGCG                 CG^CG
Asp718I       952  GGTACC               G^GTACC
AspHI         621  GAGCAC               G[AT]GC[AT]^C
BamHI         797  GGATCC               G^GATCC
BbvCI        1341  CCTCAGC              CCTCAGC(-5/-2)
BcgI          408  CGACTGGTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          700  GCCGTGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          288  GCTGAGC              GC^T[ACGT]AGC
BpmI          244  CTGGAG               CTGGAG(16/14)
Bpu1102I      288  GCTGAGC              GC^T[ACGT]AGC
BsiEI         179  CGGCCG               CG[GA][CT]^CG
BsiHKAI       621  GAGCAC               G[AT]GC[AT]^C
BsmAI        1145  GTCTC                GTCTC(1/5)
BsmI          363  GAATGC               GAATGC(1/-1)
BspHI         189  TCATGA               T^CATGA
BsrBI         788  CCGCTC               CCGCTC(-3/-3)
BsrDI        1046  GCAATG               GCAATG(2/0)
BsrFI          78  GCCGGT               [GA]^CCGG[CT]
BssSI        1150  CTCGTG               CACGAG(-5/-1)
BstAPI       1179  GCAGATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          41  CGCG                 CG^CG
BstXI        1041  CCACAGCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         288  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         78  GCCGGT               [GA]^CCGG[CT]
CpoI          668  CGGTCCG              CG^G[AT]CCG
EagI          179  CGGCCG               C^GGCCG
EarI         1309  CTCTTC               CTCTTC(1/4)
EclXI         179  CGGCCG               C^GGCCG
Eco52I        179  CGGCCG               C^GGCCG
EcoNI         914  CCTTTGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII        406  GTCGAC               GT[CT]^[GA]AC
HindII        406  GTCGAC               GT[CT]^[GA]AC
KpnI          952  GGTACC               GGTAC^C
Ksp632I      1309  CTCTTC               CTCTTC(1/4)
KspI           40  CCGCGG               CCGC^GG
MaeII        1217  ACGT                 A^CGT
MvnI           41  CGCG                 CG^CG
NdeI          311  CATATG               CA^TATG
PaeR7I         23  CTCGAG               C^TCGAG
PvuII         727  CAGCTG               CAG^CTG
RcaI          189  TCATGA               T^CATGA
RsrII         668  CGGTCCG              CG^G[AT]CCG
SacII          40  CCGCGG               CCGC^GG
SalI          406  GTCGAC               G^TCGAC
SapI         1308  GCTCTTC              GCTCTTC(1/4)
SphI           65  GCATGC               GCATG^C
XhoI           23  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          831  GTAC                 GT^AC
              953  GTAC                
AvaI           23  CTCGAG               C^[CT]CG[GA]G
              454  CTCGGG              
BalI          482  TGGCCA               TGG^CCA
              980  TGGCCA              
BanI          570  GGCACC               G^G[CT][GA]CC
              952  GGTACC              
BfaI          358  CTAG                 C^TAG
              718  CTAG                
BseRI         384  CTCCTC               GAGGAG(10/8)
             1147  CTCCTC              
BsgI         1330  CTGCAC               GTGCAG(16/14)
              874  GTGCAG              
BsoBI          23  CTCGAG               C^[CT]CG[GA]G
              454  CTCGGG              
MaeI          358  CTAG                 C^TAG
              718  CTAG                
MluNI         482  TGGCCA               TGG^CCA
              980  TGGCCA              
MscI          482  TGGCCA               TGG^CCA
              980  TGGCCA              
MslI         1042  CACAGCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1275  CATCTGGATG          
NspI           65  GCATGC               [GA]CATG^[CT]
              471  GCATGT              
PstI          277  CTGCAG               CTGCA^G
              442  CTGCAG              
RsaI          831  GTAC                 GT^AC
              953  GTAC                
SbfI          276  CCTGCAGG             CCTGCA^GG
              441  CCTGCAGG            
SfcI          277  CTGCAG               C^T[GA][CT]AG
              442  CTGCAG              
Sse8387I      276  CCTGCAGG             CCTGCA^GG
              441  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       ApaI        
AvaII       BanII       BbvI        BcnI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XhoII       
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