Restriction map for pF1KE0337
 (Circular) (Six-base) MAPSORT of: pF1KE0337.seq  Check: 3353  from: 1  to: 4640



pF1KE0337  4640 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:   1678    1678 
   Size:       4640
 
Acc65I G'GTAC_C

Cuts at:   1656    1656 
   Size:       4640
 
AclI AA'CG_TT

Cuts at:   4480    4480 
   Size:       4640
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1474    2465    2897    3644    3912    1474 
   Size:        991     432     747     268    2202

  Fragments arranged by size: 

                2202     991     747     432     268
 
AflIII A'CryG_T

Cuts at:   1992    3096    4073    1992 
   Size:       1104     977    2559

  Fragments arranged by size: 

                2559    1104     977
 
AgeI A'CCGG_T

Cuts at:   3028    3028 
   Size:       4640
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    842     842 
   Size:       4640
 
AlwNI CAG_nnn'CTG

Cuts at:    588     639    1348    1945    3512     588 
   Size:         51     709     597    1567    1716

  Fragments arranged by size: 

                1716    1567     709     597      51
 
ApaI G_GGCC'C

Cuts at:    577    1152     577 
   Size:        575    4065
 
ApaLI G'TGCA_C

Cuts at:    318    3410     318 
   Size:       3092    1548
 
ApoI r'AATT_y

Cuts at:   1644    3993    4249    1644 
   Size:       2349     256    2035

  Fragments arranged by size: 

                2349    2035     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4640
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4640
 
AvaI C'yCGr_G

Cuts at:    401     452     983    1547    1660     401 
   Size:         51     531     564     113    3381

  Fragments arranged by size: 

                3381     564     531     113      51
 
BamHI G'GATC_C

Cuts at:   1665    1665 
   Size:       4640
 
BanI G'GyrC_C

Cuts at:    120     233     390     485    1656    2070    2318    2353     120 
   Size:        113     157      95    1171     414     248      35    2407

  Fragments arranged by size: 

                2407    1171     414     248     157     113      95      35
            
 
BanII G_rGCy'C

Cuts at:    577    1152    1219    1321    1388    1654    2684     577 
   Size:        575      67     102      67     266    1030    2533

  Fragments arranged by size: 

                2533    1030     575     266     102      67      67
 
BbeI G_GCGC'C

Cuts at:   2074    2322    2074 
   Size:        248    4392
 
BbvCI CC'TCA_GC

Cuts at:   1186    1186 
   Size:       4640
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    974    1008     974 
   Size:         34    4606
 
BciVI GTATCCnnnnn_n'

Cuts at:   2538    3299    4640    2538 
   Size:        761    1341    2538

  Fragments arranged by size: 

                2538    1341     761
 
BclI T'GATC_A

Cuts at:   2160    2160 
   Size:       4640
 
BglI GCCn_nnn'nGGC

Cuts at:    690     690 
   Size:       4640
 
BglII A'GATC_T

Cuts at:   1571    2155    4192    1571 
   Size:        584    2037    2019

  Fragments arranged by size: 

                2037    2019     584
 
BlpI GC'TnA_GC

Cuts at:   1752    1752 
   Size:       4640
 
Bme1580I G_kGCm'C

Cuts at:    123     236     322     395     577    1152    1207    2265    2358 
   Size:        113      86      73     182     575      55    1058      93

Cuts at:   2358    3414     123 
   Size:       1056    1349

  Fragments arranged by size: 

                1349    1058    1056     575     182     113      93      86
                  73      55
 
BmrI ACTGGGnnnn_n'

Cuts at:   1500    1852    1933    2268    4412    1500 
   Size:        352      81     335    2144    1728

  Fragments arranged by size: 

                2144    1728     352     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    478    3007     478 
   Size:       2529    2111
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    439    1347     439 
   Size:        908    3732
 
Bpu10I CC'TnA_GC

Cuts at:   1186    1186 
   Size:       4640
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1483    1816    2695    3187    3485    1483 
   Size:        333     879     492     298    2638

  Fragments arranged by size: 

                2638     879     492     333     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    961    1006    4344     961 
   Size:         45    3338    1257

  Fragments arranged by size: 

                3338    1257      45
 
BsaAI yAC'GTr

Cuts at:    303    1993     303 
   Size:       1690    2950
 
BsaBI GATnn'nnATC

Cuts at:      6    2154    4191       6 
   Size:       2148    2037     455

  Fragments arranged by size: 

                2148    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    383     746    2071    2319    3021    4517     383 
   Size:        363    1325     248     702    1496     506

  Fragments arranged by size: 

                1496    1325     702     506     363     248
 
BsaWI w'CCGG_w

Cuts at:    997    1829    2350    3028    3302    3449    3860     997 
   Size:        832     521     678     274     147     411    1777

  Fragments arranged by size: 

                1777     832     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    124     436     124 
   Size:        312    4328
 
BseYI C'CCAG_C

Cuts at:    238     394     749    1144    1200    1437    1534    1602    2065 
   Size:        156     355     395      56     237      97      68     463

Cuts at:   2065    2823    3400    3896     238 
   Size:        758     577     496     982

  Fragments arranged by size: 

                 982     758     577     496     463     395     355     237
                 156      97      68      56
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1575    1575 
   Size:       4640
 
BsiEI CG_ry'CG

Cuts at:    104     351    2182    2228    3436     104 
   Size:        247    1831      46    1208    1308

  Fragments arranged by size: 

                1831    1308    1208     247      46
 
BsiHKAI G_wGCw'C

Cuts at:    322    1654    2432    2622    3414     322 
   Size:       1332     778     190     792    1548

  Fragments arranged by size: 

                1548    1332     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    925     925 
   Size:       4640
 
Bsp1286I G_dGCh'C

Cuts at:    123     236     322     395     577    1152    1207    1219    1321 
   Size:        113      86      73     182     575      55      12     102

Cuts at:   1321    1388    1654    2265    2358    2432    2622    2684    3414 
   Size:         67     266     611      93      74     190      62     730

Cuts at:   3414     123 
   Size:       1349

  Fragments arranged by size: 

                1349     730     611     575     266     190     182     113
                 102      93      86      74      73      67      62      55
                  12
 
BspEI T'CCGG_A

Cuts at:   3860    3860 
   Size:       4640
 
BspHI T'CATG_A

Cuts at:   1380    3816    1380 
   Size:       2436    2204
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    601    1127    1442    1690    1904    2206    2587     601 
   Size:        526     315     248     214     302     381    2654

  Fragments arranged by size: 

                2654     526     381     315     302     248     214
 
BsrBI CCG'CTC

Cuts at:     50    2932    2986    4470    4635      50 
   Size:       2882      54    1484     165      55

  Fragments arranged by size: 

                2882    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1614    2552    1614 
   Size:        938    3702
 
BsrFI r'CCGG_y

Cuts at:    346     350     475     603    2638    2819    3028     346 
   Size:          4     125     128    2035     181     209    1958

  Fragments arranged by size: 

                2035    1958     209     181     128     125       4
 
BssSI C'ACGA_G

Cuts at:   1096    2911    3269    1096 
   Size:       1815     358    2467

  Fragments arranged by size: 

                2467    1815     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1348    2017    1348 
   Size:        669    3971
 
BstBI TT'CG_AA

Cuts at:   3001    3001 
   Size:       4640
 
BstEII G'GTnAC_C

Cuts at:    426     426 
   Size:       4640
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114    1113    1542     114 
   Size:        999     429    3212

  Fragments arranged by size: 

                3212     999     429
 
BstYI r'GATC_y

Cuts at:      1    1571    1623    1665    2132    2155    2490    2736    3748 
   Size:       1570      52      42     467      23     335     246    1012

Cuts at:   3748    3834    3846    4192       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1570    1012     467     449     346     335     246      86
                  52      42      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    503     503 
   Size:       4640
 
BtgI C'CryG_G

Cuts at:    113     124     431     671     843    1263     113 
   Size:         11     307     240     172     420    3490

  Fragments arranged by size: 

                3490     420     307     240     172      11
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2590    2771    2934    2590 
   Size:        181     163    4296

  Fragments arranged by size: 

                4296     181     163
 
BtsI GCAGTG_nn'

Cuts at:    947    1153    1337    1883     947 
   Size:        206     184     546    3704

  Fragments arranged by size: 

                3704     546     206     184
 
ClaI AT'CG_AT

Cuts at:    540     540 
   Size:       4640
 
DraI TTT'AAA

Cuts at:   1639    1639 
   Size:       4640
 
DrdI GACnn_nn'nnGTC

Cuts at:   2346    3204    2346 
   Size:        858    3782
 
EaeI y'GGCC_r

Cuts at:    158     348     434     473     605     659     740    1066    1103 
   Size:        190      86      39     132      54      81     326      37

Cuts at:   1103    1448    2225    2399    2790    2817    4162     158 
   Size:        345     777     174     391      27    1345     636

  Fragments arranged by size: 

                1345     777     636     391     345     326     190     174
                 132      86      81      54      39      37      27
 
EagI C'GGCC_G

Cuts at:    348    2225     348 
   Size:       1877    2763
 
EarI CTCTTCn'nnn_

Cuts at:    923    1237    2663    2873     923 
   Size:        314    1426     210    2690

  Fragments arranged by size: 

                2690    1426     314     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    161    2848    3158    3304    4450     161 
   Size:       2687     310     146    1146     351

  Fragments arranged by size: 

                2687    1146     351     310     146
 
EcoICRI GAG'CTC

Cuts at:   1652    1652 
   Size:       4640
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    439    1347    1474    2465    2897    3644    3912     439 
   Size:        908     127     991     432     747     268    1167

  Fragments arranged by size: 

                1167     991     908     747     432     268     127
 
EcoNI CCTnn'n_nnAGG

Cuts at:    421    1408     421 
   Size:        987    3653
 
EcoO109I rG'GnC_Cy

Cuts at:    216     408     499     509     573     574     636     890     912 
   Size:        192      91      10      64       1      62     254      22

Cuts at:    912    1148    1149    1413    1557    1779     216 
   Size:        236       1     264     144     222    3077

  Fragments arranged by size: 

                3077     264     254     236     222     192     144      91
                  64      62      22      10       1       1
 
EcoRI G'AATT_C

Cuts at:   1644    1644 
   Size:       4640
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1320    2659    1320 
   Size:       1339    3301
 
FspI TGC'GCA

Cuts at:   2421    2421 
   Size:       4640
 
HaeII r_GCGC'y

Cuts at:    756    2074    2322    3344    4323     756 
   Size:       1318     248    1022     979    1073

  Fragments arranged by size: 

                1318    1073    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1563    1595    2482    2512    2514    2544    1563 
   Size:         32     887      30       2      30    3659

  Fragments arranged by size: 

                3659     887      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1679    1679 
   Size:       4640
 
KasI G'GCGC_C

Cuts at:   2070    2318    2070 
   Size:        248    4392
 
KpnI G_GTAC'C

Cuts at:   1660    1660 
   Size:       4640
 
MluI A'CGCG_T

Cuts at:   4073    4073 
   Size:       4640
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3311    3495    3311 
   Size:        184    4456
 
MscI TGG'CCA

Cuts at:    661    1068    1105    2401     661 
   Size:        407      37    1296    2900

  Fragments arranged by size: 

                2900    1296     407      37
 
MslI CAynn'nnrTG

Cuts at:    112     469     976    2629    2756     112 
   Size:        357     507    1653     127    1996

  Fragments arranged by size: 

                1996    1653     507     357     127
 
MspA1I CmG'CkG

Cuts at:    126     267     845     883    1392    1494    1519    1751    2065 
   Size:        141     578      38     509     102      25     232     314

Cuts at:   2065    2425    3438    3683     126 
   Size:        360    1013     245    1083

  Fragments arranged by size: 

                1083    1013     578     509     360     314     245     232
                 141     102      38      25
 
NaeI GCC'GGC

Cuts at:    352     477     605    2821     352 
   Size:        125     128    2216    2171

  Fragments arranged by size: 

                2216    2171     128     125
 
NarI GG'CG_CC

Cuts at:   2071    2319    2071 
   Size:        248    4392
 
NcoI C'CATG_G

Cuts at:    113     113 
   Size:       4640
 
NgoMIV G'CCGG_C

Cuts at:    350     475     603    2819     350 
   Size:        125     128    2216    2171

  Fragments arranged by size: 

                2216    2171     128     125
 
NspI r_CATG'y

Cuts at:    375    1693    2193    3100     375 
   Size:       1318     500     907    1915

  Fragments arranged by size: 

                1915    1318     907     500
 
PasI CC'CwG_GG

Cuts at:   1314    1314 
   Size:       4640
 
PciI A'CATG_T

Cuts at:   3096    3096 
   Size:       4640
 
PflMI CCAn_nnn'nTGG

Cuts at:    639    1101     639 
   Size:        462    4178
 
PmeI GTTT'AAAC

Cuts at:   1639    1639 
   Size:       4640
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3779    3811    3779 
   Size:         32    4608
 
PpuMI rG'GwC_Cy

Cuts at:    216     890     912    1413    1557     216 
   Size:        674      22     501     144    3299

  Fragments arranged by size: 

                3299     674     501     144      22
 
PshAI GACnn'nnGTC

Cuts at:    937     937 
   Size:       4640
 
PspOMI G'GGCC_C

Cuts at:    573    1148     573 
   Size:        575    4065
 
PstI C_TGCA'G

Cuts at:    954    1344    1571    1687    2372     954 
   Size:        390     227     116     685    3222

  Fragments arranged by size: 

                3222     685     390     227     116
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4640
 
PvuII CAG'CTG

Cuts at:    267     883    1494    1519    2065    2425     267 
   Size:        616     611      25     546     360    2482

  Fragments arranged by size: 

                2482     616     611     546     360      25
 
RsrII CG'GwC_CG

Cuts at:   2835    2835 
   Size:       4640
 
SacI G_AGCT'C

Cuts at:   1654    1654 
   Size:       4640
 
SacII CC_GC'GG

Cuts at:    127     846     127 
   Size:        719    3921
 
SalI G'TCGA_C

Cuts at:   1677    1677 
   Size:       4640
 
SanDI GG'GwC_CC

Cuts at:    890     890 
   Size:       4640
 
SapI GCTCTTCn'nnn_

Cuts at:   2663    2873    2663 
   Size:        210    4430
 
SbfI CC_TGCA'GG

Cuts at:   1687    1687 
   Size:       4640
 
ScaI AGT'ACT

Cuts at:   4157    4157 
   Size:       4640
 
SfcI C'TryA_G

Cuts at:     33     950    1340    1567    1683    2368    3361    3552      33 
   Size:        917     390     227     116     685     993     191    1121

  Fragments arranged by size: 

                1121     993     917     685     390     227     191     116
            
 
SfoI GGC'GCC

Cuts at:   2072    2320    2072 
   Size:        248    4392
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4640
 
SmaI CCC'GGG

Cuts at:    403    1549    1662     403 
   Size:       1146     113    3381

  Fragments arranged by size: 

                3381    1146     113
 
SmlI C'TyrA_G

Cuts at:   1498    1795    2710    3202    3464    1498 
   Size:        297     915     492     262    2674

  Fragments arranged by size: 

                2674     915     492     297     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4640
 
SphI G_CATG'C

Cuts at:   1693    2193    1693 
   Size:        500    4140
 
StyI C'CwwG_G

Cuts at:    113     489    1055    1169    1409    1774    2128     113 
   Size:        376     566     114     240     365     354    2625

  Fragments arranged by size: 

                2625     566     376     365     354     240     114
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    811    1327    2223    2356    2815    3024     811 
   Size:        516     896     133     459     209    2427

  Fragments arranged by size: 

                2427     896     516     459     209     133
 
TatI w'GTAC_w

Cuts at:    776    1292    4155     776 
   Size:        516    2863    1261

  Fragments arranged by size: 

                2863    1261     516
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4157    4189    4157 
   Size:         32    4608
 
Tth111I GACn'n_nGTC

Cuts at:   1258    2437    1258 
   Size:       1179    3461
 
XbaI T'CTAG_A

Cuts at:   1671    1671 
   Size:       4640
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    255     654     669     912    1167    2135     255 
   Size:        399      15     243     255     968    2760

  Fragments arranged by size: 

                2760     968     399     255     243      15
 
XmaI C'CCGG_G

Cuts at:    401    1547    1660     401 
   Size:       1146     113    3381

  Fragments arranged by size: 

                3381    1146     113
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     AscI
    AvrII     BaeI     BbsI    BfrBI    BmgBI     BmtI    BsaXI    BsiWI
     BsmI    BsrGI   BssHII  BstZ17I    CspCI   DraIII    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PfoI     PmlI     PsiI    PspXI     PsrI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
     XmnI     ZraI
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