Restriction map for pF1KB6382
 (Circular) (Six-base) MAPSORT of: pF1KB6382.seq  Check: 9978  from: 1  to: 4013



pF1KB6382  4013 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AccI GT'mk_AC

Cuts at:   1051    1051 
   Size:       4013
 
Acc65I G'GTAC_C

Cuts at:   1029    1029 
   Size:       4013
 
AclI AA'CG_TT

Cuts at:   3853    3853 
   Size:       4013
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    292     585    1838    2270    3017    3285     292 
   Size:        293    1253     432     747     268    1020

  Fragments arranged by size: 

                1253    1020     747     432     293     268
 
AflIII A'CryG_T

Cuts at:    842     898    1365    2469    3446     842 
   Size:         56     467    1104     977    1409

  Fragments arranged by size: 

                1409    1104     977     467      56
 
AgeI A'CCGG_T

Cuts at:   2401    2401 
   Size:       4013
 
AlwNI CAG_nnn'CTG

Cuts at:   1318    2885    1318 
   Size:       1567    2446
 
ApaLI G'TGCA_C

Cuts at:   2783    2783 
   Size:       4013
 
ApoI r'AATT_y

Cuts at:    292    1017    3366    3622     292 
   Size:        725    2349     256     683

  Fragments arranged by size: 

                2349     725     683     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4013
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4013
 
AvaI C'yCGr_G

Cuts at:    815    1033     815 
   Size:        218    3795
 
BamHI G'GATC_C

Cuts at:   1038    1038 
   Size:       4013
 
BanI G'GyrC_C

Cuts at:   1029    1443    1691    1726    1029 
   Size:        414     248      35    3316

  Fragments arranged by size: 

                3316     414     248      35
 
BanII G_rGCy'C

Cuts at:   1027    2057    1027 
   Size:       1030    2983
 
BbeI G_GCGC'C

Cuts at:   1447    1695    1447 
   Size:        248    3765
 
BbsI GAAGACnn'nnnn_

Cuts at:    591     997     591 
   Size:        406    3607
 
BciVI GTATCCnnnnn_n'

Cuts at:    370    1911    2672    4013     370 
   Size:       1541     761    1341     370

  Fragments arranged by size: 

                1541    1341     761     370
 
BclI T'GATC_A

Cuts at:   1533    1533 
   Size:       4013
 
BglII A'GATC_T

Cuts at:   1528    3565    1528 
   Size:       2037    1976
 
BlpI GC'TnA_GC

Cuts at:   1125    1125 
   Size:       4013
 
Bme1580I G_kGCm'C

Cuts at:   1638    1731    2787    1638 
   Size:         93    1056    2864

  Fragments arranged by size: 

                2864    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1225    1306    1641    3785    1225 
   Size:         81     335    2144    1453

  Fragments arranged by size: 

                2144    1453     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2380    2380 
   Size:       4013
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     748     139 
   Size:        609    3404
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    142     913    1189    2068    2560    2858     142 
   Size:        771     276     879     492     298    1297

  Fragments arranged by size: 

                1297     879     771     492     298     276
 
BsaI GGTCTCn'nnnn_

Cuts at:    550    3717     550 
   Size:       3167     846
 
BsaAI yAC'GTr

Cuts at:    901    1366     901 
   Size:        465    3548
 
BsaBI GATnn'nnATC

Cuts at:      6    1527    3564       6 
   Size:       1521    2037     455

  Fragments arranged by size: 

                2037    1521     455
 
BsaHI Gr'CG_yC

Cuts at:    746    1444    1692    2394    3890     746 
   Size:        698     248     702    1496     869

  Fragments arranged by size: 

                1496     869     702     698     248
 
BsaWI w'CCGG_w

Cuts at:    189    1202    1723    2401    2675    2822    3233     189 
   Size:       1013     521     678     274     147     411     969

  Fragments arranged by size: 

                1013     969     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    157     713     157 
   Size:        556    3457
 
BseYI C'CCAG_C

Cuts at:   1438    2196    2773    3269    1438 
   Size:        758     577     496    2182

  Fragments arranged by size: 

                2182     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    844     844 
   Size:       4013
 
BsiEI CG_ry'CG

Cuts at:    104     176    1555    1601    2809     104 
   Size:         72    1379      46    1208    1308

  Fragments arranged by size: 

                1379    1308    1208      72      46
 
BsiHKAI G_wGCw'C

Cuts at:    816     880    1027    1805    1995    2787     816 
   Size:         64     147     778     190     792    2042

  Fragments arranged by size: 

                2042     792     778     190     147      64
 
BsmBI CGTCTCn'nnnn_

Cuts at:    139     139 
   Size:       4013
 
Bsp1286I G_dGCh'C

Cuts at:    816     880    1027    1638    1731    1805    1995    2057    2787 
   Size:         64     147     611      93      74     190      62     730

Cuts at:   2787     816 
   Size:       2042

  Fragments arranged by size: 

                2042     730     611     190     147      93      74      64
                  62
 
BspEI T'CCGG_A

Cuts at:   3233    3233 
   Size:       4013
 
BspHI T'CATG_A

Cuts at:   3189    3189 
   Size:       4013
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    866    1063    1277    1579    1960     866 
   Size:        197     214     302     381    2919

  Fragments arranged by size: 

                2919     381     302     214     197
 
BsrBI CCG'CTC

Cuts at:     50    2305    2359    3843    4008      50 
   Size:       2255      54    1484     165      55

  Fragments arranged by size: 

                2255    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1925    1925 
   Size:       4013
 
BsrFI r'CCGG_y

Cuts at:   2011    2192    2401    2011 
   Size:        181     209    3623

  Fragments arranged by size: 

                3623     209     181
 
BssSI C'ACGA_G

Cuts at:   2284    2642    2284 
   Size:        358    3655
 
BstAPI GCAn_nnn'nTGC

Cuts at:    319    1390     319 
   Size:       1071    2942
 
BstBI TT'CG_AA

Cuts at:   2374    2374 
   Size:       4013
 
BstYI r'GATC_y

Cuts at:      1    1038    1505    1528    1863    2109    3121    3207    3219 
   Size:       1037     467      23     335     246    1012      86      12

Cuts at:   3219    3565       1 
   Size:        346     449

  Fragments arranged by size: 

                1037    1012     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1963    2144    2307    1963 
   Size:        181     163    3669

  Fragments arranged by size: 

                3669     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1256    1256 
   Size:       4013
 
DraI TTT'AAA

Cuts at:   1012    1012 
   Size:       4013
 
DraIII CAC_nnn'GTG

Cuts at:    319     319 
   Size:       4013
 
DrdI GACnn_nn'nnGTC

Cuts at:    631    1719    2577     631 
   Size:       1088     858    2067

  Fragments arranged by size: 

                2067    1088     858
 
EaeI y'GGCC_r

Cuts at:    173    1598    1772    2163    2190    3535     173 
   Size:       1425     174     391      27    1345     651

  Fragments arranged by size: 

                1425    1345     651     391     174      27
 
EagI C'GGCC_G

Cuts at:    173    1598     173 
   Size:       1425    2588
 
EarI CTCTTCn'nnn_

Cuts at:    500    2036    2246     500 
   Size:       1536     210    2267

  Fragments arranged by size: 

                2267    1536     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2221    2531    2677    3823    2221 
   Size:        310     146    1146    2411

  Fragments arranged by size: 

                2411    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1025    1025 
   Size:       4013
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     292     585     748    1838    2270    3017    3285     139 
   Size:        153     293     163    1090     432     747     268     867

  Fragments arranged by size: 

                1090     867     747     432     293     268     163     153
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    118     118 
   Size:       4013
 
EcoO109I rG'GnC_Cy

Cuts at:    228     699    1152     228 
   Size:        471     453    3089

  Fragments arranged by size: 

                3089     471     453
 
EcoRI G'AATT_C

Cuts at:   1017    1017 
   Size:       4013
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2032    2032 
   Size:       4013
 
FspI TGC'GCA

Cuts at:   1794    1794 
   Size:       4013
 
HaeII r_GCGC'y

Cuts at:   1447    1695    2717    3696    1447 
   Size:        248    1022     979    1764

  Fragments arranged by size: 

                1764    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    368     400    1855    1885    1887    1917     368 
   Size:         32    1455      30       2      30    2464

  Fragments arranged by size: 

                2464    1455      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1052    1052 
   Size:       4013
 
KasI G'GCGC_C

Cuts at:   1443    1691    1443 
   Size:        248    3765
 
KpnI G_GTAC'C

Cuts at:   1033    1033 
   Size:       4013
 
MluI A'CGCG_T

Cuts at:   3446    3446 
   Size:       4013
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2684    2868    2684 
   Size:        184    3829
 
MscI TGG'CCA

Cuts at:   1774    1774 
   Size:       4013
 
MslI CAynn'nnrTG

Cuts at:    803     901    2002    2129     803 
   Size:         98    1101     127    2687

  Fragments arranged by size: 

                2687    1101     127      98
 
MspA1I CmG'CkG

Cuts at:    751    1124    1438    1798    2811    3056     751 
   Size:        373     314     360    1013     245    1708

  Fragments arranged by size: 

                1708    1013     373     360     314     245
 
NaeI GCC'GGC

Cuts at:   2194    2194 
   Size:       4013
 
NarI GG'CG_CC

Cuts at:   1444    1692    1444 
   Size:        248    3765
 
NgoMIV G'CCGG_C

Cuts at:   2192    2192 
   Size:       4013
 
NotI GC'GGCC_GC

Cuts at:    173     173 
   Size:       4013
 
NspI r_CATG'y

Cuts at:    453    1066    1566    2473     453 
   Size:        613     500     907    1993

  Fragments arranged by size: 

                1993     907     613     500
 
PciI A'CATG_T

Cuts at:   2469    2469 
   Size:       4013
 
PfoI T'CCnGG_A

Cuts at:    848     848 
   Size:       4013
 
PmeI GTTT'AAAC

Cuts at:   1012    1012 
   Size:       4013
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3152    3184    3152 
   Size:         32    3981
 
PpuMI rG'GwC_Cy

Cuts at:    228     699     228 
   Size:        471    3542
 
PstI C_TGCA'G

Cuts at:   1060    1745    1060 
   Size:        685    3328
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4013
 
PvuII CAG'CTG

Cuts at:   1438    1798    1438 
   Size:        360    3653
 
RsrII CG'GwC_CG

Cuts at:   2208    2208 
   Size:       4013
 
SacI G_AGCT'C

Cuts at:   1027    1027 
   Size:       4013
 
SalI G'TCGA_C

Cuts at:   1050    1050 
   Size:       4013
 
SapI GCTCTTCn'nnn_

Cuts at:   2036    2246    2036 
   Size:        210    3803
 
SbfI CC_TGCA'GG

Cuts at:   1060    1060 
   Size:       4013
 
ScaI AGT'ACT

Cuts at:   3530    3530 
   Size:       4013
 
SfcI C'TryA_G

Cuts at:     33    1056    1741    2734    2925      33 
   Size:       1023     685     993     191    1121

  Fragments arranged by size: 

                1121    1023     993     685     191
 
SfoI GGC'GCC

Cuts at:   1445    1693    1445 
   Size:        248    3765
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4013
 
SmaI CCC'GGG

Cuts at:   1035    1035 
   Size:       4013
 
SmlI C'TyrA_G

Cuts at:    157     815     928    1168    2083    2575    2837     157 
   Size:        658     113     240     915     492     262    1333

  Fragments arranged by size: 

                1333     915     658     492     262     240     113
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4013
 
SphI G_CATG'C

Cuts at:   1066    1566    1066 
   Size:        500    3513
 
StuI AGG'CCT

Cuts at:    886     886 
   Size:       4013
 
StyI C'CwwG_G

Cuts at:    422    1147    1501     422 
   Size:        725     354    2934

  Fragments arranged by size: 

                2934     725     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    745    1596    1729    2188    2397     745 
   Size:        851     133     459     209    2361

  Fragments arranged by size: 

                2361     851     459     209     133
 
TatI w'GTAC_w

Cuts at:   3528    3528 
   Size:       4013
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3530    3562    3530 
   Size:         32    3981
 
Tth111I GACn'n_nGTC

Cuts at:    586    1810     586 
   Size:       1224    2789
 
XbaI T'CTAG_A

Cuts at:   1044    1044 
   Size:       4013
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114    1508     114 
   Size:       1394    2619
 
XhoI C'TCGA_G

Cuts at:    815     815 
   Size:       4013
 
XmaI C'CCGG_G

Cuts at:   1033    1033 
   Size:       4013
 
XmnI GAAnn'nnTTC

Cuts at:    410     410 
   Size:       4013
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NotI     NspI     PciI
     PfoI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI   Bpu10I    BsaXI    BsiWI     BsmI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI
     NruI     NsiI     PacI     PasI    PflMI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     SwaI     ZraI
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