Restriction map for pF1KB6382
 (Linear) (Six-base) MAPSORT of: KIBB6382.seq  Check: 6958  from: 1  to: 900



KIBB6382.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     477     900 
   Size:        184     293     423

  Fragments arranged by size: 

                 423     293     184
 
AflIII A'CryG_T

Cuts at:      0     734     790     900 
   Size:        734      56     110

  Fragments arranged by size: 

                 734     110      56
 
ApoI r'AATT_y

Cuts at:      0     184     900 
   Size:        184     716
 
AvaI C'yCGr_G

Cuts at:      0     707     900 
   Size:        707     193
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     483     889     900 
   Size:        483     406      11

  Fragments arranged by size: 

                 483     406      11
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     262     900 
   Size:        262     638
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     640     900 
   Size:         31     609     260

  Fragments arranged by size: 

                 609     260      31
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     805     900 
   Size:         34     771      95

  Fragments arranged by size: 

                 771      95      34
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     442     900 
   Size:        442     458
 
BsaAI yAC'GTr

Cuts at:      0     793     900 
   Size:        793     107
 
BsaHI Gr'CG_yC

Cuts at:      0     638     900 
   Size:        638     262
 
BsaWI w'CCGG_w

Cuts at:      0      81     900 
   Size:         81     819
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49     605     900 
   Size:         49     556     295

  Fragments arranged by size: 

                 556     295      49
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     736     900 
   Size:        736     164
 
BsiEI CG_ry'CG

Cuts at:      0      68     900 
   Size:         68     832
 
BsiHKAI G_wGCw'C

Cuts at:      0     708     772     900 
   Size:        708      64     128

  Fragments arranged by size: 

                 708     128      64
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      31     900 
   Size:         31     869
 
Bsp1286I G_dGCh'C

Cuts at:      0     708     772     900 
   Size:        708      64     128

  Fragments arranged by size: 

                 708     128      64
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     758     900 
   Size:        758     142
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     211     900 
   Size:        211     689
 
DraIII CAC_nnn'GTG

Cuts at:      0     211     900 
   Size:        211     689
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     523     900 
   Size:        523     377
 
EaeI y'GGCC_r

Cuts at:      0      65     900 
   Size:         65     835
 
EagI C'GGCC_G

Cuts at:      0      65     900 
   Size:         65     835
 
EarI CTCTTCn'nnn_

Cuts at:      0     392     900 
   Size:        392     508
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     184     477     640     900 
   Size:         31     153     293     163     260

  Fragments arranged by size: 

                 293     260     163     153      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      10     900 
   Size:         10     890
 
EcoO109I rG'GnC_Cy

Cuts at:      0     120     591     900 
   Size:        120     471     309

  Fragments arranged by size: 

                 471     309     120
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     260     292     900 
   Size:        260      32     608

  Fragments arranged by size: 

                 608     260      32
 
MslI CAynn'nnrTG

Cuts at:      0     695     793     900 
   Size:        695      98     107

  Fragments arranged by size: 

                 695     107      98
 
MspA1I CmG'CkG

Cuts at:      0     643     900 
   Size:        643     257
 
NotI GC'GGCC_GC

Cuts at:      0      65     900 
   Size:         65     835
 
NspI r_CATG'y

Cuts at:      0     345     900 
   Size:        345     555
 
PfoI T'CCnGG_A

Cuts at:      0     740     900 
   Size:        740     160
 
PpuMI rG'GwC_Cy

Cuts at:      0     120     591     900 
   Size:        120     471     309

  Fragments arranged by size: 

                 471     309     120
 
SmlI C'TyrA_G

Cuts at:      0      49     707     820     900 
   Size:         49     658     113      80

  Fragments arranged by size: 

                 658     113      80      49
 
StuI AGG'CCT

Cuts at:      0     778     900 
   Size:        778     122
 
StyI C'CwwG_G

Cuts at:      0     314     900 
   Size:        314     586
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     637     900 
   Size:        637     263
 
Tth111I GACn'n_nGTC

Cuts at:      0     478     900 
   Size:        478     422
 
XhoI C'TCGA_G

Cuts at:      0     707     900 
   Size:        707     193
 
XmnI GAAnn'nnTTC

Cuts at:      0     302     900 
   Size:        302     598
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AvaI     BbsI    BciVI     BpmI    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspMI   BstAPI   DraIII     DrdI     EaeI     EagI
     EarI  Eco57MI    EcoNI EcoO109I    Hin4I     MslI   MspA1I     NotI
     NspI     PfoI    PpuMI     SmlI     StuI     StyI    TaqII  Tth111I
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaBI    BsaXI    BseYI
    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI     TstI     XbaI     XcmI
     XmaI     ZraI
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