Restriction map for pF1KB6345
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
BpmI        Bpu1102I    BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        HindIII     
HpaI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       Tsp509I     Tth111I     TthHB8I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcyI          289  GGCGCC               G[GA]^CG[CT]C
AlwNI         172  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          625  GGGCCC               GGGCC^C
Asp700I       588  GAATCGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          15  GTGCTC               G[AT]GC[AT]^C
AvaI          338  CCCGGG               C^[CT]CG[GA]G
BbeI          289  GGCGCC               GGCGC^C
BglI          467  GCCCTGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         242  AGATCT               A^GATCT
BsaHI         289  GGCGCC               G[GA]^CG[CT]C
BseRI         748  GAGGAG               GAGGAG(10/8)
BsiHKAI        15  GTGCTC               G[AT]GC[AT]^C
BsmFI         180  GGGAC                GGGAC(10/14)
BsoBI         338  CCCGGG               C^[CT]CG[GA]G
BspHI         584  TCATGA               T^CATGA
BspMI         668  ACCTGC               ACCTGC(4/8)
BsrDI         294  CATTGC               GCAATG(2/0)
BstYI         242  AGATCT               [GA]^GATC[CT]
Hin1I         289  GGCGCC               G[GA]^CG[CT]C
HincII        355  GTCAAC               GT[CT]^[GA]AC
HindII        355  GTCAAC               GT[CT]^[GA]AC
KasI          289  GGCGCC               G^GCGCC
MaeIII        505  GTCAC                ^GT[ACGT]AC
MflI          242  AGATCT               [GA]^GATC[CT]
MslI          393  CATGGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          289  GGCGCC               GG^CGCC
PleI          451  GACTC                GAGTC(4/5)
PpuMI         180  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          127  CTGCAG               CTGCA^G
RcaI          584  TCATGA               T^CATGA
SfcI          127  CTGCAG               C^T[GA][CT]AG
SfoI          289  GGCGCC               GGC^GCC
SmaI          338  CCCGGG               CCC^GGG
Tsp45I        505  GTCAC                ^GT[GC]AC
XcmI          155  CCATAGGCACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         242  AGATCT               [GA]^GATC[CT]
XmaCI         338  CCCGGG               C^CCGGG
XmaI          338  CCCGGG               C^CCGGG
XmnI          588  GAATCGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII         135  CGCG                 CG^CG
              360  CGCG                
AvaII         181  GGACC                G^G[AT]CC
              642  GGTCC               
BanI          289  GGCGCC               G^G[CT][GA]CC
              324  GGCACC              
BbsI          771  GAAGAC               GAAGAC(2/6)
               73  GTCTTC              
BpuAI         771  GAAGAC               GAAGAC(2/6)
               73  GTCTTC              
BsaAI         268  TACGTG               [CT]AC^GT[GA]
              723  CACGTA              
BstUI         135  CGCG                 CG^CG
              360  CGCG                
DdeI          688  CTGAG                C^T[ACGT]AG
              694  CTGAG               
DpnII         132  GATC                 ^GATC
              243  GATC                
EarI          673  CTCTTC               CTCTTC(1/4)
              760  GAAGAG              
EcoT14I       392  CCATGG               C^C[AT][AT]GG
              776  CCATGG              
HaeII         111  GGCGCT               [GA]GCGC^[CT]
              289  GGCGCC              
HphI          215  TCACC                GGTGA(8/7)
              506  TCACC               
Ksp632I       673  CTCTTC               CTCTTC(1/4)
              760  GAAGAG              
MaeII         269  ACGT                 A^CGT
              724  ACGT                
MboI          132  GATC                 ^GATC
              243  GATC                
MvnI          135  CGCG                 CG^CG
              360  CGCG                
NcoI          392  CCATGG               C^CATGG
              776  CCATGG              
NdeII         132  GATC                 ^GATC
              243  GATC                
PvuII         280  CAGCTG               CAG^CTG
              421  CAGCTG              
SapI          760  GAAGAGC              GCTCTTC(1/4)
              672  GCTCTTC             
Sau3AI        132  GATC                 ^GATC
              243  GATC                
SfaNI         308  GCATC                GCATC(5/9)
              434  GCATC               
SmlI           31  CTCAAG               C^T[CT][GA]AG
               97  CTCAAG              
StyI          392  CCATGG               C^C[AT][AT]GG
              776  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AluI        BalI        BanII       BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BsrBI       BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       CfoI        Cfr13I      
DraII       DsaI        EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
MboII       MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       PflMI       Sau96I      ScrFI       TfiI        
TseI        TspRI       Van91I      
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