Restriction map for pF1KB6345
 (Linear) (Six-base) MAPSORT of: KIBB6345.seq  Check: 3179  from: 1  to: 795



KIBB6345.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     177     795 
   Size:        177     618
 
ApaI G_GGCC'C

Cuts at:      0     629     795 
   Size:        629     166
 
AvaI C'yCGr_G

Cuts at:      0     338     795 
   Size:        338     457
 
BanI G'GyrC_C

Cuts at:      0     289     324     795 
   Size:        289      35     471

  Fragments arranged by size: 

                 471     289      35
 
BanII G_rGCy'C

Cuts at:      0      91     478     629     795 
   Size:         91     387     151     166

  Fragments arranged by size: 

                 387     166     151      91
 
BbeI G_GCGC'C

Cuts at:      0     293     795 
   Size:        293     502
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      66     778     795 
   Size:         66     712      17

  Fragments arranged by size: 

                 712      66      17
 
BglI GCCn_nnn'nGGC

Cuts at:      0     473     795 
   Size:        473     322
 
BglII A'GATC_T

Cuts at:      0     242     795 
   Size:        242     553
 
Bme1580I G_kGCm'C

Cuts at:      0     629     795 
   Size:        629     166
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     618     753     795 
   Size:        618     135      42

  Fragments arranged by size: 

                 618     135      42
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      33     795 
   Size:         33     762
 
Bpu10I CC'TnA_GC

Cuts at:      0     688     795 
   Size:        688     107
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16      82     795 
   Size:         16      66     713

  Fragments arranged by size: 

                 713      66      16
 
BsaAI yAC'GTr

Cuts at:      0     270     725     795 
   Size:        270     455      70

  Fragments arranged by size: 

                 455     270      70
 
BsaHI Gr'CG_yC

Cuts at:      0     290     795 
   Size:        290     505
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     763     795 
   Size:        763      32
 
BseYI C'CCAG_C

Cuts at:      0     278     480     555     795 
   Size:        278     202      75     240

  Fragments arranged by size: 

                 278     240     202      75
 
BsiHKAI G_wGCw'C

Cuts at:      0      19     795 
   Size:         19     776
 
Bsp1286I G_dGCh'C

Cuts at:      0      19      91     478     629     795 
   Size:         19      72     387     151     166

  Fragments arranged by size: 

                 387     166     151      72      19
 
BspHI T'CATG_A

Cuts at:      0     584     795 
   Size:        584     211
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     677     795 
   Size:        677     118
 
BsrBI CCG'CTC

Cuts at:      0     191     330     563     604     765     795 
   Size:        191     139     233      41     161      30

  Fragments arranged by size: 

                 233     191     161     139      41      30
 
BsrDI GCAATG_nn'

Cuts at:      0     293     795 
   Size:        293     502
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     219     399     471     795 
   Size:        219     180      72     324

  Fragments arranged by size: 

                 324     219     180      72
 
BstYI r'GATC_y

Cuts at:      0     242     795 
   Size:        242     553
 
BtgI C'CryG_G

Cuts at:      0      20     392     776     795 
   Size:         20     372     384      19

  Fragments arranged by size: 

                 384     372      20      19
 
EaeI y'GGCC_r

Cuts at:      0      56     103     152     195     350     707     795 
   Size:         56      47      49      43     155     357      88

  Fragments arranged by size: 

                 357     155      88      56      49      47      43
 
EarI CTCTTCn'nnn_

Cuts at:      0     679     755     795 
   Size:        679      76      40

  Fragments arranged by size: 

                 679      76      40
 
EcoO109I rG'GnC_Cy

Cuts at:      0     174     181     625     795 
   Size:        174       7     444     170

  Fragments arranged by size: 

                 444     174     170       7
 
HaeII r_GCGC'y

Cuts at:      0     115     293     795 
   Size:        115     178     502

  Fragments arranged by size: 

                 502     178     115
 
HincII GTy'rAC

Cuts at:      0     357     795 
   Size:        357     438
 
KasI G'GCGC_C

Cuts at:      0     289     795 
   Size:        289     506
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     504     795 
   Size:        504     291
 
MscI TGG'CCA

Cuts at:      0      58     154     709     795 
   Size:         58      96     555      86

  Fragments arranged by size: 

                 555      96      86      58
 
MslI CAynn'nnrTG

Cuts at:      0     397     795 
   Size:        397     398
 
MspA1I CmG'CkG

Cuts at:      0     282     423     756     795 
   Size:        282     141     333      39

  Fragments arranged by size: 

                 333     282     141      39
 
NarI GG'CG_CC

Cuts at:      0     290     795 
   Size:        290     505
 
NcoI C'CATG_G

Cuts at:      0     392     776     795 
   Size:        392     384      19

  Fragments arranged by size: 

                 392     384      19
 
PasI CC'CwG_GG

Cuts at:      0     177     510     795 
   Size:        177     333     285

  Fragments arranged by size: 

                 333     285     177
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     177     285     393     795 
   Size:        177     108     108     402

  Fragments arranged by size: 

                 402     177     108     108
 
PpuMI rG'GwC_Cy

Cuts at:      0     181     795 
   Size:        181     614
 
PspOMI G'GGCC_C

Cuts at:      0     625     795 
   Size:        625     170
 
PstI C_TGCA'G

Cuts at:      0     131     795 
   Size:        131     664
 
PvuII CAG'CTG

Cuts at:      0     282     423     795 
   Size:        282     141     372

  Fragments arranged by size: 

                 372     282     141
 
SanDI GG'GwC_CC

Cuts at:      0     181     795 
   Size:        181     614
 
SapI GCTCTTCn'nnn_

Cuts at:      0     679     755     795 
   Size:        679      76      40

  Fragments arranged by size: 

                 679      76      40
 
SfcI C'TryA_G

Cuts at:      0     127     795 
   Size:        127     668
 
SfoI GGC'GCC

Cuts at:      0     291     795 
   Size:        291     504
 
SmaI CCC'GGG

Cuts at:      0     340     795 
   Size:        340     455
 
SmlI C'TyrA_G

Cuts at:      0      31      97     795 
   Size:         31      66     698

  Fragments arranged by size: 

                 698      66      31
 
StyI C'CwwG_G

Cuts at:      0     392     776     795 
   Size:        392     384      19

  Fragments arranged by size: 

                 392     384      19
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     162     795 
   Size:        162     633
 
XmaI C'CCGG_G

Cuts at:      0     338     795 
   Size:        338     457
 
XmnI GAAnn'nnTTC

Cuts at:      0     592     795 
   Size:        592     203
 

 Enzymes that do cut:

    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BbsI     BglI
    BglII Bme1580I     BmrI     BplI   Bpu10I    BpuEI    BsaAI    BsaHI
    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BstXI    BstYI     BtgI     EaeI     EarI EcoO109I    HaeII   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     PasI
    PflMI    PpuMI   PspOMI     PstI    PvuII    SanDI     SapI     SfcI
     SfoI     SmaI     SmlI     StyI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BlpI    BmgBI     BmtI     BpmI     BsaI
    BsaBI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KpnI
     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     ZraI
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