Restriction map for pF1KA1428
 (Linear) (Six-base) MAPSORT of: KIAA1428.seq  Check: 986  from: 1  to: 2127



KIAA1428.orf

 With 182 enzymes: SgfI * 

                        October 16, 2009 16:19  ..

AccI GT'mk_AC

Cuts at:      0    1303    2022    2127 
   Size:       1303     719     105

  Fragments arranged by size: 

                1303     719     105
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547    1499    2127 
   Size:        547     952     628

  Fragments arranged by size: 

                 952     628     547
 
AflII C'TTAA_G

Cuts at:      0    1134    2127 
   Size:       1134     993
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     680     712    2127 
   Size:        680      32    1415

  Fragments arranged by size: 

                1415     680      32
 
ApoI r'AATT_y

Cuts at:      0     670     808     825     921    1308    2127 
   Size:        670     138      17      96     387     819

  Fragments arranged by size: 

                 819     670     387     138      96      17
 
AseI AT'TA_AT

Cuts at:      0     431    2127 
   Size:        431    1696
 
AvaI C'yCGr_G

Cuts at:      0    1592    2127 
   Size:       1592     535
 
BamHI G'GATC_C

Cuts at:      0    1285    2127 
   Size:       1285     842
 
BanII G_rGCy'C

Cuts at:      0     250    2127 
   Size:        250    1877
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1013    2127 
   Size:       1013    1114
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     838    2127 
   Size:        838    1289
 
BclI T'GATC_A

Cuts at:      0     414    2127 
   Size:        414    1713
 
BfrBI ATG'CAT

Cuts at:      0     132     537    2127 
   Size:        132     405    1590

  Fragments arranged by size: 

                1590     405     132
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1424    2127 
   Size:       1424     703
 
BglII A'GATC_T

Cuts at:      0     363    2127 
   Size:        363    1764
 
BlpI GC'TnA_GC

Cuts at:      0     286    2127 
   Size:        286    1841
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     342    2127 
   Size:        342    1785
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     306    2127 
   Size:        306    1821
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58    1475    2127 
   Size:         58    1417     652

  Fragments arranged by size: 

                1417     652      58
 
Bpu10I CC'TnA_GC

Cuts at:      0    2062    2127 
   Size:       2062      65
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     727    2127 
   Size:        727    1400
 
BsaBI GATnn'nnATC

Cuts at:      0    1547    2127 
   Size:       1547     580
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43    2127 
   Size:         43    2084
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185    1145    2127 
   Size:        185     960     982

  Fragments arranged by size: 

                 982     960     185
 
BsiHKAI G_wGCw'C

Cuts at:      0     250    2127 
   Size:        250    1877
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      25    2127 
   Size:         25    2102
 
Bsp1286I G_dGCh'C

Cuts at:      0     250    2127 
   Size:        250    1877
 
BspHI T'CATG_A

Cuts at:      0     417    1086    2127 
   Size:        417     669    1041

  Fragments arranged by size: 

                1041     669     417
 
BsrDI GCAATG_nn'

Cuts at:      0     253    2127 
   Size:        253    1874
 
BsrGI T'GTAC_A

Cuts at:      0     500    1102    2127 
   Size:        500     602    1025

  Fragments arranged by size: 

                1025     602     500
 
BssSI C'ACGA_G

Cuts at:      0    1163    1222    2127 
   Size:       1163      59     905

  Fragments arranged by size: 

                1163     905      59
 
BstYI r'GATC_y

Cuts at:      0     363    1097    1285    1294    2127 
   Size:        363     734     188       9     833

  Fragments arranged by size: 

                 833     734     363     188       9
 
Bsu36I CC'TnA_GG

Cuts at:      0    1290    2127 
   Size:       1290     837
 
BtgI C'CryG_G

Cuts at:      0     939     965    2127 
   Size:        939      26    1162

  Fragments arranged by size: 

                1162     939      26
 
BtsI GCAGTG_nn'

Cuts at:      0     308    2127 
   Size:        308    1819
 
DraI TTT'AAA

Cuts at:      0     194     354    1655    2127 
   Size:        194     160    1301     472

  Fragments arranged by size: 

                1301     472     194     160
 
EaeI y'GGCC_r

Cuts at:      0     962    1234    1255    1398    1416    2127 
   Size:        962     272      21     143      18     711

  Fragments arranged by size: 

                 962     711     272     143      21      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     566    1085    2075    2104    2127 
   Size:        566     519     990      29      23

  Fragments arranged by size: 

                 990     566     519      29      23
 
EcoICRI GAG'CTC

Cuts at:      0     248    2127 
   Size:        248    1879
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     547    1475    1499    2127 
   Size:         58     489     928      24     628

  Fragments arranged by size: 

                 928     628     489      58      24
 
HaeII r_GCGC'y

Cuts at:      0    1746    2127 
   Size:       1746     381
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1522    1554    2127 
   Size:       1522      32     573

  Fragments arranged by size: 

                1522     573      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     125    1836    2127 
   Size:        125    1711     291

  Fragments arranged by size: 

                1711     291     125
 
MscI TGG'CCA

Cuts at:      0     964    1400    1418    2127 
   Size:        964     436      18     709

  Fragments arranged by size: 

                 964     709     436      18
 
MslI CAynn'nnrTG

Cuts at:      0    1553    2127 
   Size:       1553     574
 
NcoI C'CATG_G

Cuts at:      0     965    2127 
   Size:        965    1162
 
NsiI A_TGCA'T

Cuts at:      0     134     539    2127 
   Size:        134     405    1588

  Fragments arranged by size: 

                1588     405     134
 
NspI r_CATG'y

Cuts at:      0     612    1885    2127 
   Size:        612    1273     242

  Fragments arranged by size: 

                1273     612     242
 
SacI G_AGCT'C

Cuts at:      0     250    2127 
   Size:        250    1877
 
SmaI CCC'GGG

Cuts at:      0    1594    2127 
   Size:       1594     533
 
SmlI C'TyrA_G

Cuts at:      0    1134    2127 
   Size:       1134     993
 
SphI G_CATG'C

Cuts at:      0    1885    2127 
   Size:       1885     242
 
SrfI GCCC'GGGC

Cuts at:      0    1594    2127 
   Size:       1594     533
 
SspI AAT'ATT

Cuts at:      0     684    2127 
   Size:        684    1443
 
StuI AGG'CCT

Cuts at:      0     959    2127 
   Size:        959    1168
 
StyI C'CwwG_G

Cuts at:      0     965    1518    2127 
   Size:        965     553     609

  Fragments arranged by size: 

                 965     609     553
 
TatI w'GTAC_w

Cuts at:      0     500     566    1102    1462    2127 
   Size:        500      66     536     360     665

  Fragments arranged by size: 

                 665     536     500     360      66
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     795     827    2127 
   Size:        795      32    1300

  Fragments arranged by size: 

                1300     795      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      57    2127 
   Size:         57    2070
 
XbaI T'CTAG_A

Cuts at:      0    1326    2127 
   Size:       1326     801
 
XmaI C'CCGG_G

Cuts at:      0    1592    2127 
   Size:       1592     535
 
XmnI GAAnn'nnTTC

Cuts at:      0     395    2127 
   Size:        395    1732
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AloI     ApoI     AseI     AvaI    BamHI
    BanII     BbsI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BseRI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrDI    BsrGI    BssSI    BstYI
   Bsu36I     BtgI     BtsI     DraI     EaeI     EarI  EcoICRI  Eco57MI
    HaeII    Hin4I     MmeI     MscI     MslI     NcoI     NsiI     NspI
     SacI     SmaI     SmlI     SphI     SrfI     SspI     StuI     StyI
     TatI     TstI  Tth111I     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI     BanI     BbeI    BbvCI     BcgI Bme1580I    BmgBI     BmtI
    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI
     BsmI    BspEI    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SwaI    TaqII     XcmI     XhoI     ZraI
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