Restriction map for pF1KE0435
##################################
# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        Eam1105I    Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PstI        PvuI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tsp45I      Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcyI          639  GGCGTC               G[GA]^CG[CT]C
Aor51HI       761  AGCGCT               AGC^GCT
AspHI         661  GTGCTC               G[AT]GC[AT]^C
AvaII         193  GGACC                G^G[AT]CC
BanI          670  GGCACC               G^G[CT][GA]CC
BbsI           64  GTCTTC               GAAGAC(2/6)
BpmI          765  CTCCAG               CTGGAG(16/14)
BpuAI          64  GTCTTC               GAAGAC(2/6)
BsaHI         639  GGCGTC               G[GA]^CG[CT]C
BseRI         455  CTCCTC               GAGGAG(10/8)
BsiEI           8  CGGCCG               CG[GA][CT]^CG
BsiHKAI       661  GTGCTC               G[AT]GC[AT]^C
BsmFI         147  GTCCC                GGGAC(10/14)
BspMI         587  ACCTGC               ACCTGC(4/8)
BsrDI          33  CATTGC               GCAATG(2/0)
BsrI          290  CCAGT                ACTGG(1/-1)
BstF5I        561  CATCC                GGATG(2/0)
DdeI          684  CTTAG                C^T[ACGT]AG
DpnII         216  GATC                 ^GATC
EaeI            8  CGGCCG               [CT]^GGCC[GA]
EagI            8  CGGCCG               C^GGCCG
EarI          303  GAAGAG               CTCTTC(1/4)
EclXI           8  CGGCCG               C^GGCCG
Eco47III      761  AGCGCT               AGC^GCT
Eco52I          8  CGGCCG               C^GGCCG
EcoT14I       419  CCTTGG               C^C[AT][AT]GG
FauI          691  GCGGG                CCCGC(4/6)
FokI          561  CATCC                GGATG(9/13)
HaeII         761  AGCGCT               [GA]GCGC^[CT]
HapII         110  CCGG                 C^CGG
Hin1I         639  GGCGTC               G[GA]^CG[CT]C
HpaII         110  CCGG                 C^CGG
Ksp632I       303  GAAGAG               CTCTTC(1/4)
MboI          216  GATC                 ^GATC
MseI          280  TTAA                 T^TAA
MspA1I        699  CAGCTG               C[AC]G^C[GT]G
MspI          110  CCGG                 C^CGG
NdeII         216  GATC                 ^GATC
NspI          507  GCATGT               [GA]CATG^[CT]
PflMI         585  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         486  GACGCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         699  CAGCTG               CAG^CTG
Sau3AI        216  GATC                 ^GATC
SfaNI         560  GCATC                GCATC(5/9)
StyI          419  CCTTGG               C^C[AT][AT]GG
Tru9I         280  TTAA                 T^TAA
Tsp509I       461  AATT                 ^AATT
Van91I        585  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AciI            7  GCGG                 CCGC(-3/-1)
              691  GCGG                
BmyI          661  GTGCTC               G[AGT]GC[ACT]^C
              669  GGGCAC              
BsiYI         149  CCCTTGTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              585  CCACCTGCTGG         
BslI          149  CCCTTGTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              585  CCACCTGCTGG         
BsmI          440  GCATTC               GAATGC(1/-1)
              679  GCATTC              
Bsp1286I      661  GTGCTC               G[AGT]GC[ACT]^C
              669  GGGCAC              
BspHI         377  TCATGA               T^CATGA
              482  TCATGA              
HgaI          486  GACGC                GACGC(5/10)
              640  GCGTC               
HphI          319  GGTGA                GGTGA(8/7)
               68  TCACC               
NlaIV         423  GGGGCC               GG[ACGT]^[ACGT]CC
              670  GGCACC              
PleI          415  GACTC                GAGTC(4/5)
              170  GAGTC               
RcaI          377  TCATGA               T^CATGA
              482  TCATGA              
TfiI          374  GAATC                G^A[AT]TC
              451  GATTC               
TspRI         291  CAGTG                CA[GC]TG[ACGT][ACGT]^
              434  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsmAI       BssKI       
BstNI       Cac8I       CfoI        Cfr13I      EcoRII      
Fnu4HI      HaeIII      HhaI        HinP1I      HinfI       
ItaI        MboII       MnlI        MvaI        MwoI        
NlaIII      Sau96I      ScrFI       TseI        
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