Restriction map for pF1KB8998
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BclI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BsgI        
BsiWI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BssHII      Bst1107I    BstAPI      BstBI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraII       DraIII      DrdI        Eam1105I    Eco47III    
EcoO109I    EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        TaqI        Tru9I       Tth111I     
TthHB8I     XbaI        XhoI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AlwI          596  GGATC                GGATC(4/5)
AlwNI         640  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          921  CCTGAGG              CC^T[ACGT]AGG
Asp700I       849  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          962  CCCGGG               C^[CT]CG[GA]G
BciVI         872  GTATCC               GTATCC(6/5)
BsaWI         329  TCCGGT               [AT]^CCGG[AT]
BseRI          15  CTCCTC               GAGGAG(10/8)
BsiEI         149  CGGCCG               CG[GA][CT]^CG
BsmBI        1009  CGTCTC               CGTCTC(1/5)
BsoBI         962  CCCGGG               C^[CT]CG[GA]G
Bsp1407I       23  TGTACA               T^GTACA
BsrFI         349  ACCGGC               [GA]^CCGG[CT]
BsrGI          23  TGTACA               T^GTACA
BssSI         354  CACGAG               CACGAG(-5/-1)
BstEII        345  GGTGACC              G^GT[ACGT]ACC
BstPI         345  GGTGACC              G^GT[ACGT]ACC
BstYI         596  GGATCT               [GA]^GATC[CT]
Bsu36I        921  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        349  ACCGGC               [GA]^CCGG[CT]
DsaI          222  CCACGG               C^C[GA][CT]GG
EagI          149  CGGCCG               C^GGCCG
EarI          232  CTCTTC               CTCTTC(1/4)
EclXI         149  CGGCCG               C^GGCCG
Eco52I        149  CGGCCG               C^GGCCG
Eco81I        921  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1209  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       345  GGTGACC              G^GT[ACGT]ACC
Ksp632I       232  CTCTTC               CTCTTC(1/4)
MflI          596  GGATCT               [GA]^GATC[CT]
PflMI         377  CCATCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmaI          962  CCCGGG               CCC^GGG
SmlI         1168  CTCAAG               C^T[CT][GA]AG
SspBI          23  TGTACA               T^GTACA
TfiI          849  GAATC                G^A[AT]TC
Tsp509I       838  AATT                 ^AATT
Van91I        377  CCATCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         596  GGATCT               [GA]^GATC[CT]
XmaCI         962  CCCGGG               C^CCGGG
XmaI          962  CCCGGG               C^CCGGG
XmnI          849  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII         611  CGCG                 CG^CG
              877  CGCG                
BanI          561  GGTGCC               G^G[CT][GA]CC
              952  GGCACC              
BfaI          421  CTAG                 C^TAG
             1198  CTAG                
BsmFI         514  GTCCC                GGGAC(10/14)
             1119  GTCCC               
BspMI         107  ACCTGC               ACCTGC(4/8)
              996  GCAGGT              
BstUI         611  CGCG                 CG^CG
              877  CGCG                
CfoI          212  GCGC                 GCG^C
              610  GCGC                
DpnII         597  GATC                 ^GATC
              903  GATC                
HhaI          212  GCGC                 GCG^C
              610  GCGC                
HinP1I        212  GCGC                 G^CGC
              610  GCGC                
MaeI          421  CTAG                 C^TAG
             1198  CTAG                
MboI          597  GATC                 ^GATC
              903  GATC                
MspA1I        619  CAGCTG               C[AC]G^C[GT]G
              947  CAGCTG              
MvnI          611  CGCG                 CG^CG
              877  CGCG                
NdeII         597  GATC                 ^GATC
              903  GATC                
PleI          411  GACTC                GAGTC(4/5)
             1117  GAGTC               
PvuII         619  CAGCTG               CAG^CTG
              947  CAGCTG              
Sau3AI        597  GATC                 ^GATC
              903  GATC                
SfaNI         320  GCATC                GCATC(5/9)
              368  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       AvaII       
BalI        BanII       BbvI        BcnI        BmyI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BsrBI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstXI       Cac8I       Cfr13I      
DdeI        EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboII       
MluNI       MnlI        MscI        MslI        MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PstI        RsaI        SacI        Sau96I      ScrFI       
SfcI        TseI        Tsp45I      TspRI       XcmI        
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