Restriction map for
pF1KB7223
(Circular) (Six-base) MAPSORT of: pF1KB7223.seq Check: 8689 from: 1 to: 4628
pF1KB7223 4628 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 1666 1666
Size: 4628
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 1134 1567 2453 2885 3632 3900 582
Size: 552 433 886 432 747 268 1310
Fragments arranged by size:
1310 886 747 552 433 432 268
AflIII A'CryG_T
Cuts at: 702 1980 3084 4061 702
Size: 1278 1104 977 1269
Fragments arranged by size:
1278 1269 1104 977
AgeI A'CCGG_T
Cuts at: 3016 3016
Size: 4628
AlwNI CAG_nnn'CTG
Cuts at: 469 1933 3500 469
Size: 1464 1567 1597
Fragments arranged by size:
1597 1567 1464
ApaLI G'TGCA_C
Cuts at: 3398 3398
Size: 4628
ApoI r'AATT_y
Cuts at: 284 478 610 1023 1183 1632 3981 4237 284
Size: 194 132 413 160 449 2349 256 675
Fragments arranged by size:
2349 675 449 413 256 194 160 132
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 1648 1648
Size: 4628
BamHI G'GATC_C
Cuts at: 1653 1653
Size: 4628
BanI G'GyrC_C
Cuts at: 894 1644 2058 2306 2341 894
Size: 750 414 248 35 3181
Fragments arranged by size:
3181 750 414 248 35
BanII G_rGCy'C
Cuts at: 322 1642 2672 322
Size: 1320 1030 2278
Fragments arranged by size:
2278 1320 1030
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BbsI GAAGACnn'nnnn_
Cuts at: 699 973 699
Size: 274 4354
BciVI GTATCCnnnnn_n'
Cuts at: 248 522 2526 3287 4628 248
Size: 274 2004 761 1341 248
Fragments arranged by size:
2004 1341 761 274 248
BclI T'GATC_A
Cuts at: 1478 2148 1478
Size: 670 3958
BfrBI ATG'CAT
Cuts at: 1532 1532
Size: 4628
BglII A'GATC_T
Cuts at: 344 1437 2143 4180 344
Size: 1093 706 2037 792
Fragments arranged by size:
2037 1093 792 706
BlpI GC'TnA_GC
Cuts at: 1740 1740
Size: 4628
Bme1580I G_kGCm'C
Cuts at: 2253 2346 3402 2253
Size: 93 1056 3479
Fragments arranged by size:
3479 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1560 1840 1921 2256 4400 1560
Size: 280 81 335 2144 1788
Fragments arranged by size:
2144 1788 335 280 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 4628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1804 2683 3175 3473 1804
Size: 879 492 298 2959
Fragments arranged by size:
2959 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4332 4332
Size: 4628
BsaAI yAC'GTr
Cuts at: 1981 1981
Size: 4628
BsaBI GATnn'nnATC
Cuts at: 6 1483 2142 4179 6
Size: 1477 659 2037 455
Fragments arranged by size:
2037 1477 659 455
BsaHI Gr'CG_yC
Cuts at: 2059 2307 3009 4505 2059
Size: 248 702 1496 2182
Fragments arranged by size:
2182 1496 702 248
BsaWI w'CCGG_w
Cuts at: 275 1817 2338 3016 3290 3437 3848 275
Size: 1542 521 678 274 147 411 1055
Fragments arranged by size:
1542 1055 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1565 1630 1565
Size: 65 4563
BseYI C'CCAG_C
Cuts at: 426 837 859 1070 1397 1452 2053 2811 3388
Size: 411 22 211 327 55 601 758 577
Cuts at: 3388 3884 426
Size: 496 1170
Fragments arranged by size:
1170 758 601 577 496 411 327 211
55 22
BsiEI CG_ry'CG
Cuts at: 104 2170 2216 3424 104
Size: 2066 46 1208 1308
Fragments arranged by size:
2066 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 299 1642 2420 2610 3402 299
Size: 1343 778 190 792 1525
Fragments arranged by size:
1525 1343 792 778 190
BsmI GAATG_Cn'
Cuts at: 823 941 1003 1066 823
Size: 118 62 63 4385
Fragments arranged by size:
4385 118 63 62
Bsp1286I G_dGCh'C
Cuts at: 299 322 1642 2253 2346 2420 2610 2672 3402
Size: 23 1320 611 93 74 190 62 730
Cuts at: 3402 299
Size: 1525
Fragments arranged by size:
1525 1320 730 611 190 93 74 62
23
BspEI T'CCGG_A
Cuts at: 3848 3848
Size: 4628
BspHI T'CATG_A
Cuts at: 3804 3804
Size: 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1678 1892 2194 2575 1678
Size: 214 302 381 3731
Fragments arranged by size:
3731 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2920 2974 4458 4623 50
Size: 2870 54 1484 165 55
Fragments arranged by size:
2870 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1093 2540 1093
Size: 1447 3181
BsrFI r'CCGG_y
Cuts at: 2626 2807 3016 2626
Size: 181 209 4238
Fragments arranged by size:
4238 209 181
BsrGI T'GTAC_A
Cuts at: 1198 1198
Size: 4628
BssSI C'ACGA_G
Cuts at: 2899 3257 2899
Size: 358 4270
BstAPI GCAn_nnn'nTGC
Cuts at: 2005 2005
Size: 4628
BstBI TT'CG_AA
Cuts at: 2989 2989
Size: 4628
BstEII G'GTnAC_C
Cuts at: 948 948
Size: 4628
BstYI r'GATC_y
Cuts at: 1 344 1437 1653 2120 2143 2478 2724 3736
Size: 343 1093 216 467 23 335 246 1012
Cuts at: 3736 3822 3834 4180 1
Size: 86 12 346 449
Fragments arranged by size:
1093 1012 467 449 346 343 335 246
216 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4628
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 187 1871 187
Size: 1684 2944
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 2334 3192 2334
Size: 858 3770
EaeI y'GGCC_r
Cuts at: 376 1256 2213 2387 2778 2805 4150 376
Size: 880 957 174 391 27 1345 854
Fragments arranged by size:
1345 957 880 854 391 174 27
EagI C'GGCC_G
Cuts at: 2213 2213
Size: 4628
EarI CTCTTCn'nnn_
Cuts at: 780 808 2651 2861 780
Size: 28 1843 210 2547
Fragments arranged by size:
2547 1843 210 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 946 2836 3146 3292 4438 946
Size: 1890 310 146 1146 1136
Fragments arranged by size:
1890 1146 1136 310 146
EcoICRI GAG'CTC
Cuts at: 1640 1640
Size: 4628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 1134 1567 2453 2885 3632 3900 582
Size: 552 433 886 432 747 268 1310
Fragments arranged by size:
1310 886 747 552 433 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1767 1767
Size: 4628
EcoRI G'AATT_C
Cuts at: 1632 1632
Size: 4628
EcoRV GAT'ATC
Cuts at: 1553 1553
Size: 4628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4628
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 2062 2310 3332 4311 2062
Size: 248 1022 979 2379
Fragments arranged by size:
2379 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 960 992 2470 2500 2502 2532 960
Size: 32 1478 30 2 30 3056
Fragments arranged by size:
3056 1478 32 30 30 2
HincII GTy'rAC
Cuts at: 1667 1667
Size: 4628
HindIII A'AGCT_T
Cuts at: 166 1292 166
Size: 1126 3502
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 635 3299 3483 635
Size: 2664 184 1780
Fragments arranged by size:
2664 1780 184
MscI TGG'CCA
Cuts at: 378 2389 378
Size: 2011 2617
MslI CAynn'nnrTG
Cuts at: 2617 2744 2617
Size: 127 4501
MspA1I CmG'CkG
Cuts at: 430 1154 1452 1739 2053 2413 3426 3671 430
Size: 724 298 287 314 360 1013 245 1387
Fragments arranged by size:
1387 1013 724 360 314 298 287 245
NaeI GCC'GGC
Cuts at: 2809 2809
Size: 4628
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NcoI C'CATG_G
Cuts at: 107 107
Size: 4628
NgoMIV G'CCGG_C
Cuts at: 2807 2807
Size: 4628
NsiI A_TGCA'T
Cuts at: 1534 1534
Size: 4628
NspI r_CATG'y
Cuts at: 706 1681 2181 3088 706
Size: 975 500 907 2246
Fragments arranged by size:
2246 975 907 500
PciI A'CATG_T
Cuts at: 702 3084 702
Size: 2382 2246
PflMI CCAn_nnn'nTGG
Cuts at: 844 844
Size: 4628
PfoI T'CCnGG_A
Cuts at: 1113 1113
Size: 4628
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 4596
PstI C_TGCA'G
Cuts at: 1022 1675 2360 1022
Size: 653 685 3290
Fragments arranged by size:
3290 685 653
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 430 1154 1452 2053 2413 430
Size: 724 298 601 360 2645
Fragments arranged by size:
2645 724 601 360 298
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 1642 1642
Size: 4628
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 1147 4145 1147
Size: 2998 1630
SfcI C'TryA_G
Cuts at: 33 470 1018 1035 1371 1671 2356 3349 3540
Size: 437 548 17 336 300 685 993 191
Cuts at: 3540 33
Size: 1121
Fragments arranged by size:
1121 993 685 548 437 336 300 191
17
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 1650 1650
Size: 4628
SmlI C'TyrA_G
Cuts at: 1783 2698 3190 3452 1783
Size: 915 492 262 2959
Fragments arranged by size:
2959 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1681 2181 1681
Size: 500 4128
SspI AAT'ATT
Cuts at: 264 264
Size: 4628
StyI C'CwwG_G
Cuts at: 107 843 988 1762 2116 107
Size: 736 145 774 354 2619
Fragments arranged by size:
2619 774 736 354 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2211 2344 2803 3012 2211
Size: 133 459 209 3827
Fragments arranged by size:
3827 459 209 133
TatI w'GTAC_w
Cuts at: 1145 1167 1198 4143 1145
Size: 22 31 2945 1630
Fragments arranged by size:
2945 1630 31 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 496 528 1116 1148 4145 4177 496
Size: 32 588 32 2997 32 947
Fragments arranged by size:
2997 947 588 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2425 2425
Size: 4628
XbaI T'CTAG_A
Cuts at: 559 1659 559
Size: 1100 3528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 2123
Size: 4628
XmaI C'CCGG_G
Cuts at: 1648 1648
Size: 4628
XmnI GAAnn'nnTTC
Cuts at: 454 454
Size: 4628
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI