Restriction map for pF1KB7223
 (Linear) (Six-base) MAPSORT of: KIBB7223.seq  Check: 5662  from: 1  to: 1515



KIBB7223.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     474    1026    1459    1515 
   Size:        474     552     433      56

  Fragments arranged by size: 

                 552     474     433      56
 
AflIII A'CryG_T

Cuts at:      0     594    1515 
   Size:        594     921
 
AlwNI CAG_nnn'CTG

Cuts at:      0     361    1515 
   Size:        361    1154
 
ApoI r'AATT_y

Cuts at:      0     176     370     502     915    1075    1515 
   Size:        176     194     132     413     160     440

  Fragments arranged by size: 

                 440     413     194     176     160     132
 
BanI G'GyrC_C

Cuts at:      0     786    1515 
   Size:        786     729
 
BanII G_rGCy'C

Cuts at:      0     214    1515 
   Size:        214    1301
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     591     865    1515 
   Size:        591     274     650

  Fragments arranged by size: 

                 650     591     274
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     140     414    1515 
   Size:        140     274    1101

  Fragments arranged by size: 

                1101     274     140
 
BclI T'GATC_A

Cuts at:      0    1370    1515 
   Size:       1370     145
 
BfrBI ATG'CAT

Cuts at:      0    1424    1515 
   Size:       1424      91
 
BglII A'GATC_T

Cuts at:      0     236    1329    1515 
   Size:        236    1093     186

  Fragments arranged by size: 

                1093     236     186
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1452    1515 
   Size:       1452      63
 
BsaBI GATnn'nnATC

Cuts at:      0    1375    1515 
   Size:       1375     140
 
BsaWI w'CCGG_w

Cuts at:      0     167    1515 
   Size:        167    1348
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1457    1515 
   Size:       1457      58
 
BseYI C'CCAG_C

Cuts at:      0     318     729     751     962    1289    1344    1515 
   Size:        318     411      22     211     327      55     171

  Fragments arranged by size: 

                 411     327     318     211     171      55      22
 
BsiHKAI G_wGCw'C

Cuts at:      0     191    1515 
   Size:        191    1324
 
BsmI GAATG_Cn'

Cuts at:      0     715     833     895     958    1515 
   Size:        715     118      62      63     557

  Fragments arranged by size: 

                 715     557     118      63      62
 
Bsp1286I G_dGCh'C

Cuts at:      0     191     214    1515 
   Size:        191      23    1301

  Fragments arranged by size: 

                1301     191      23
 
BsrDI GCAATG_nn'

Cuts at:      0     985    1515 
   Size:        985     530
 
BsrGI T'GTAC_A

Cuts at:      0    1090    1515 
   Size:       1090     425
 
BstEII G'GTnAC_C

Cuts at:      0     840    1515 
   Size:        840     675
 
BstYI r'GATC_y

Cuts at:      0     236    1329    1515 
   Size:        236    1093     186

  Fragments arranged by size: 

                1093     236     186
 
BtsI GCAGTG_nn'

Cuts at:      0      79    1515 
   Size:         79    1436
 
EaeI y'GGCC_r

Cuts at:      0     268    1148    1515 
   Size:        268     880     367

  Fragments arranged by size: 

                 880     367     268
 
EarI CTCTTCn'nnn_

Cuts at:      0     672     700    1515 
   Size:        672      28     815

  Fragments arranged by size: 

                 815     672      28
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     838    1515 
   Size:        838     677
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     474    1026    1459    1515 
   Size:        474     552     433      56

  Fragments arranged by size: 

                 552     474     433      56
 
EcoRV GAT'ATC

Cuts at:      0    1445    1515 
   Size:       1445      70
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     852     884    1515 
   Size:        852      32     631

  Fragments arranged by size: 

                 852     631      32
 
HindIII A'AGCT_T

Cuts at:      0      58    1184    1515 
   Size:         58    1126     331

  Fragments arranged by size: 

                1126     331      58
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     527    1515 
   Size:        527     988
 
MscI TGG'CCA

Cuts at:      0     270    1515 
   Size:        270    1245
 
MspA1I CmG'CkG

Cuts at:      0     322    1046    1344    1515 
   Size:        322     724     298     171

  Fragments arranged by size: 

                 724     322     298     171
 
NsiI A_TGCA'T

Cuts at:      0    1426    1515 
   Size:       1426      89
 
NspI r_CATG'y

Cuts at:      0     598    1515 
   Size:        598     917
 
PciI A'CATG_T

Cuts at:      0     594    1515 
   Size:        594     921
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     736    1515 
   Size:        736     779
 
PfoI T'CCnGG_A

Cuts at:      0    1005    1515 
   Size:       1005     510
 
PstI C_TGCA'G

Cuts at:      0     914    1515 
   Size:        914     601
 
PvuII CAG'CTG

Cuts at:      0     322    1046    1344    1515 
   Size:        322     724     298     171

  Fragments arranged by size: 

                 724     322     298     171
 
ScaI AGT'ACT

Cuts at:      0    1039    1515 
   Size:       1039     476
 
SfcI C'TryA_G

Cuts at:      0     362     910     927    1263    1515 
   Size:        362     548      17     336     252

  Fragments arranged by size: 

                 548     362     336     252      17
 
SspI AAT'ATT

Cuts at:      0     156    1515 
   Size:        156    1359
 
StyI C'CwwG_G

Cuts at:      0     735     880    1515 
   Size:        735     145     635

  Fragments arranged by size: 

                 735     635     145
 
TatI w'GTAC_w

Cuts at:      0    1037    1059    1090    1515 
   Size:       1037      22      31     425

  Fragments arranged by size: 

                1037     425      31      22
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     388     420    1008    1040    1515 
   Size:        388      32     588      32     475

  Fragments arranged by size: 

                 588     475     388      32      32
 
XbaI T'CTAG_A

Cuts at:      0     451    1515 
   Size:        451    1064
 
XmnI GAAnn'nnTTC

Cuts at:      0     346    1515 
   Size:        346    1169
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     BanI    BanII     BbsI    BciVI
     BclI    BfrBI    BglII     BmrI    BsaBI    BsaWI    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrGI   BstEII    BstYI     BtsI
     EaeI     EarI     EciI  Eco57MI    EcoRV    Hin4I  HindIII     MmeI
     MscI   MspA1I     NsiI     NspI     PciI    PflMI     PfoI     PstI
    PvuII     ScaI     SfcI     SspI     StyI     TatI     TstI     XbaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII  Tth111I     XcmI
     XhoI     XmaI     ZraI
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