Restriction map for pF1KB7328
##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       AclI        AflII       AgeI        Alw44I      
AocI        ApaLI       AscI        AseI        AsnI        
AspI        AviII       BbeI        BbvCI       BcgI        
BciVI       BfrI        BglI        BspDI       BspLU11I    
BssHII      Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
Eco81I      EcoRI       EcoT22I     FseI        FspI        
HpaI        KasI        KspI        MfeI        MunI        
NarI        NcoI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SbfI        SexAI       SfiI        
SfoI        SfuI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       2372  GGTACC               G^GTACC
AccIII       3472  TCCGGA               T^CCGGA
AhdI         1882  GACCCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      2422  AGCGCT               AGC^GCT
Asp700I      3464  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2372  GGTACC               G^GTACC
AspEI        1882  GACCCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         3803  CCCGAG               C^[CT]CG[GA]G
AvrII        1578  CCTAGG               C^CTAGG
BanI         2372  GGTACC               G^G[CT][GA]CC
BlnI         1578  CCTAGG               C^CTAGG
BlpI         3195  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3195  GCTGAGC              GC^T[ACGT]AGC
BsaBI         372  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        3472  TCCGGA               T^CCGGA
BsiWI         758  CGTACG               C^GTACG
BsoBI        3803  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     2561  TGTACA               T^GTACA
BspEI        3472  TCCGGA               T^CCGGA
BsrGI        2561  TGTACA               T^GTACA
BssSI        2821  CTCGTG               CACGAG(-5/-1)
CelII        3195  GCTGAGC              GC^T[ACGT]AGC
DraIII        337  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1892  CGGCCG               C^GGCCG
Eam1105I     1882  GACCCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1892  CGGCCG               C^GGCCG
Eco47III     2422  AGCGCT               AGC^GCT
Eco52I       1892  CGGCCG               C^GGCCG
HincII        240  GTCGAC               GT[CT]^[GA]AC
HindII        240  GTCGAC               GT[CT]^[GA]AC
HindIII      1497  AAGCTT               A^AGCTT
KpnI         2372  GGTACC               GGTAC^C
MamI          372  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          363  ACGCGT               A^CGCGT
MroI         3472  TCCGGA               T^CCGGA
NaeI           47  GCCGGC               GCC^GGC
NdeI          245  CATATG               CA^TATG
NgoMIV         47  GCCGGC               G^CCGGC
NspI         2544  GCATGT               [GA]CATG^[CT]
SalI          240  GTCGAC               G^TCGAC
SapI         3808  GCTCTTC              GCTCTTC(1/4)
ScaI         3154  AGTACT               AGT^ACT
SgrAI         182  CGCCGGTG             C[GA]^CCGG[CT]G
SmlI         2926  CTCAAG               C^T[CT][GA]AG
SspBI        2561  TGTACA               T^GTACA
XmnI         3464  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI         1900  GACGCC               G[GA]^CG[CT]C
             2596  GGCGTC              
AflIII        363  ACGCGT               A^C[GA][CT]GT
             2348  ACACGT              
BbrPI         337  CACGTG               CAC^GTG
             1906  CACGTG              
BclI          932  TGATCA               T^GATCA
             1701  TGATCA              
BcnI          132  CCCGG                CC^[GC]GG
              146  CCGGG               
BsaHI        1900  GACGCC               G[GA]^CG[CT]C
             2596  GGCGTC              
BsaI         1772  GAGACC               GGTCTC(1/5)
             2449  GGTCTC              
BsaWI         575  TCCGGT               [AT]^CCGG[AT]
             3472  TCCGGA              
BsgI         1486  CTGCAC               GTGCAG(16/14)
             3915  CTGCAC              
BsiEI        1857  CGACCG               CG[GA][CT]^CG
             1892  CGGCCG              
BsmBI        1104  GAGACG               CGTCTC(1/5)
             3235  GAGACG              
BsmFI        3322  GGGAC                GGGAC(10/14)
             3329  GTCCC               
BsmI         3298  GAATGC               GAATGC(1/-1)
             3557  GCATTC              
BspHI        3095  TCATGA               T^CATGA
             3701  TCATGA              
BsrBI        1792  GAGCGG               CCGCTC(-3/-3)
             3198  GAGCGG              
EcoRV         798  GATATC               GAT^ATC
             3550  GATATC              
FauI         1841  CCCGC                CCCGC(4/6)
             1794  GCGGG               
FbaI          932  TGATCA               T^GATCA
             1701  TGATCA              
Hin1I        1900  GACGCC               G[GA]^CG[CT]C
             2596  GGCGTC              
NciI          132  CCCGG                CC^[GC]GG
              146  CCGGG               
PflMI        3332  CCAAGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3517  CCAGCCAGTGG         
PmaCI         337  CACGTG               CAC^GTG
             1906  CACGTG              
PmlI          337  CACGTG               CAC^GTG
             1906  CACGTG              
PshAI        1959  GACAGTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2533  GACATCGGTC          
PstI         1594  CTGCAG               CTGCA^G
             2791  CTGCAG              
RcaI         3095  TCATGA               T^CATGA
             3701  TCATGA              
StuI          323  AGGCCT               AGG^CCT
             3168  AGGCCT              
Van91I       3332  CCAAGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3517  CCAGCCAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AspHI       AvaII       BalI        BamHI       BanII       
BbsI        BbvI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaAI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT14I     Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
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