Restriction map for pF1KA0822
##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

Acc65I      AclI        AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       BbeI        BcgI        BciVI       
BlpI        Bpu1102I    BsgI        BsiWI       BsmBI       
BspLU11I    BssHII      BstEII      BstPI       CelII       
CpoI        DraI        DraII       EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO109I    EcoO65I     
FseI        HgaI        HpaI        KasI        KpnI        
KspI        MluI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PinAI       PmeI        PpuMI       PshAI       
Psp1406I    PvuI        RsrII       SacII       SbfI        
ScaI        SexAI       SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        1289  GACGTC               GACGT^C
AccIII       3052  TCCGGA               T^CCGGA
AcyI         1289  GACGTC               G[GA]^CG[CT]C
AflII        2013  CTTAAG               C^TTAAG
AspI         4128  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         4228  CCCGAG               C^[CT]CG[GA]G
AviII        2416  TGCGCA               TGC^GCA
AvrII        2190  CCTAGG               C^CTAGG
BalI         4343  TGGCCA               TGG^CCA
BanI         4288  GGTGCC               G^G[CT][GA]CC
BbrPI        1348  CACGTG               CAC^GTG
BfrI         2013  CTTAAG               C^TTAAG
BglI         4255  GCCATCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         2190  CCTAGG               C^CTAGG
BsaHI        1289  GACGTC               G[GA]^CG[CT]C
BsaI         1325  GGTCTC               GGTCTC(1/5)
BseAI        3052  TCCGGA               T^CCGGA
BsiEI        4214  CGACCG               CG[GA][CT]^CG
BsoBI        4228  CCCGAG               C^[CT]CG[GA]G
BspDI        3010  ATCGAT               AT^CGAT
BspEI        3052  TCCGGA               T^CCGGA
BsrBI        3933  GAGCGG               CCGCTC(-3/-3)
BsrFI         988  ACCGGC               [GA]^CCGG[CT]
BssSI        3795  CACGAG               CACGAG(-5/-1)
BstAPI       2797  GCATGCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         182  CCATGGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        988  ACCGGC               [GA]^CCGG[CT]
ClaI         3010  ATCGAT               AT^CGAT
DrdI         1283  GACATCGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         4343  TGGCCA               [CT]^GGCC[GA]
EcoRV        2006  GATATC               GAT^ATC
FauI         1966  GCGGG                CCCGC(4/6)
FspI         2416  TGCGCA               TGC^GCA
Hin1I        1289  GACGTC               G[GA]^CG[CT]C
MfeI         2420  CAATTG               C^AATTG
MluNI        4343  TGGCCA               TGG^CCA
MroI         3052  TCCGGA               T^CCGGA
MscI         4343  TGGCCA               TGG^CCA
MunI         2420  CAATTG               C^AATTG
PflFI        4128  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1348  CACGTG               CAC^GTG
PmlI         1348  CACGTG               CAC^GTG
PstI         4099  CTGCAG               CTGCA^G
SalI         4212  GTCGAC               G^TCGAC
SfcI         4099  CTGCAG               C^T[GA][CT]AG
SfiI         4254  GGCCATCCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         2797  GCATGC               GCATG^C
StuI          755  AGGCCT               AGG^CCT
Tth111I      4128  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         4301  CCACCCACTACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AocI         3973  CCTCAGG              CC^T[ACGT]AGG
             4476  CCTGAGG             
BamHI        1821  GGATCC               G^GATCC
             3573  GGATCC              
BclI         1344  TGATCA               T^GATCA
             2627  TGATCA              
BcnI         4217  CCGGG                CC^[GC]GG
             4260  CCGGG               
BglII         865  AGATCT               A^GATCT
             1500  AGATCT              
BsaAI        1348  CACGTG               [CT]AC^GT[GA]
             3241  TACGTG              
BsmI          431  GAATGC               GAATGC(1/-1)
              633  GCATTC              
Bsp1407I     1046  TGTACA               T^GTACA
             3468  TGTACA              
BspHI        1389  TCATGA               T^CATGA
             3233  TCATGA              
BsrGI        1046  TGTACA               T^GTACA
             3468  TGTACA              
Bst1107I      232  GTATAC               GTA^TAC
             2520  GTATAC              
BstBI        3157  TTCGAA               TT^CGAA
             3349  TTCGAA              
BstZ17I       232  GTATAC               GTA^TAC
             2520  GTATAC              
Bsu36I       3973  CCTCAGG              CC^T[ACGT]AGG
             4476  CCTGAGG             
DraIII        583  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2757  CACAGTGTG           
DsaI          182  CCATGG               C^C[GA][CT]GG
             2525  CCATGG              
Eco81I       3973  CCTCAGG              CC^T[ACGT]AGG
             4476  CCTGAGG             
FbaI         1344  TGATCA               T^GATCA
             2627  TGATCA              
HaeII        1407  AGCGCC               [GA]GCGC^[CT]
             3087  GGCGCT              
MspA1I       3726  CAGCTG               C[AC]G^C[GT]G
             4108  CAGCTG              
NciI         4217  CCGGG                CC^[GC]GG
             4260  CCGGG               
NcoI          182  CCATGG               C^CATGG
             2525  CCATGG              
NspI         2797  GCATGC               [GA]CATG^[CT]
             3164  ACATGC              
NspV         3157  TTCGAA               TT^CGAA
             3349  TTCGAA              
PvuII        3726  CAGCTG               CAG^CTG
             4108  CAGCTG              
RcaI         1389  TCATGA               T^CATGA
             3233  TCATGA              
SacI         3029  GAGCTC               GAGCT^C
             4657  GAGCTC              
SfuI         3157  TTCGAA               TT^CGAA
             3349  TTCGAA              
SmlI         2013  CTTAAG               C^T[CT][GA]AG
             4093  CTCAAG              
SspBI        1046  TGTACA               T^GTACA
             3468  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApoI        AseI        
AsnI        Asp700I     AspHI       AvaII       BanII       
BbsI        BbvCI       BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaBI       BsaJI       BsaWI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EarI        EcoNI       EcoRI       EcoRII      EcoT14I     
EcoT22I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       NsiI        PflMI       PleI        
PshBI       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmnI        
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