Restriction map for pF1KA0822
 (Linear) (Six-base) MAPSORT of: KIAA0822.seq  Check: 7634  from: 1  to: 4743



KIAA0822.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3399    4743 
   Size:       3399    1344
 
AatII G_ACGT'C

Cuts at:      0    1293    4743 
   Size:       1293    3450
 
AccI GT'mk_AC

Cuts at:      0     233    2521    3732    4213    4633    4743 
   Size:        233    2288    1211     481     420     110

  Fragments arranged by size: 

                2288    1211     481     420     233     110
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     891    1330    1872    2263    3473    4132    4743 
   Size:        383     508     439     542     391    1210     659     611

  Fragments arranged by size: 

                1210     659     611     542     508     439     391     383
            
 
AflII C'TTAA_G

Cuts at:      0    2013    4743 
   Size:       2013    2730
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     775     807    4743 
   Size:        775      32    3936

  Fragments arranged by size: 

                3936     775      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     613    3090    4017    4321    4677    4743 
   Size:        613    2477     927     304     356      66

  Fragments arranged by size: 

                2477     927     613     356     304      66
 
ApoI r'AATT_y

Cuts at:      0     105     343    1149    1203    1262    1417    2149    3587 
   Size:        105     238     806      54      59     155     732    1438

Cuts at:   3587    3687    4402    4743 
   Size:        100     715     341

  Fragments arranged by size: 

                1438     806     732     715     341     238     155     105
                 100      59      54
 
AseI AT'TA_AT

Cuts at:      0     551    2267    2482    4743 
   Size:        551    1716     215    2261

  Fragments arranged by size: 

                2261    1716     551     215
 
AvaI C'yCGr_G

Cuts at:      0    4228    4743 
   Size:       4228     515
 
AvrII C'CTAG_G

Cuts at:      0    2190    4743 
   Size:       2190    2553
 
BamHI G'GATC_C

Cuts at:      0    1821    3573    4743 
   Size:       1821    1752    1170

  Fragments arranged by size: 

                1821    1752    1170
 
BanI G'GyrC_C

Cuts at:      0    4288    4743 
   Size:       4288     455
 
BanII G_rGCy'C

Cuts at:      0    1972    3033    4460    4661    4743 
   Size:       1972    1061    1427     201      82

  Fragments arranged by size: 

                1972    1427    1061     201      82
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     769     897    3111    3167    4132    4743 
   Size:        769     128    2214      56     965     611

  Fragments arranged by size: 

                2214     965     769     611     128      56
 
BbvCI CC'TCA_GC

Cuts at:      0    4045    4061    4322    4743 
   Size:       4045      16     261     421

  Fragments arranged by size: 

                4045     421     261      16
 
BclI T'GATC_A

Cuts at:      0    1344    2627    4743 
   Size:       1344    1283    2116

  Fragments arranged by size: 

                2116    1344    1283
 
BfrBI ATG'CAT

Cuts at:      0     633     718    1159    2707    4743 
   Size:        633      85     441    1548    2036

  Fragments arranged by size: 

                2036    1548     633     441      85
 
BglI GCCn_nnn'nGGC

Cuts at:      0    4261    4743 
   Size:       4261     482
 
BglII A'GATC_T

Cuts at:      0     865    1500    4743 
   Size:        865     635    3243

  Fragments arranged by size: 

                3243     865     635
 
Bme1580I G_kGCm'C

Cuts at:      0    4293    4743 
   Size:       4293     450
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     867    1890    2821    4186    4743 
   Size:        867    1023     931    1365     557

  Fragments arranged by size: 

                1365    1023     931     867     557
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1750    4743 
   Size:       1750    2993
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1397    3911    3973    4039    4444    4660    4675    4743 
   Size:       1397    2514      62      66     405     216      15      68

  Fragments arranged by size: 

                2514    1397     405     216      68      66      62      15
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    4045    4061    4168    4322    4743 
   Size:       4045      16     107     154     421

  Fragments arranged by size: 

                4045     421     154     107      16
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    4078    4743 
   Size:       4078     665
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1331    4743 
   Size:       1331    3412
 
BsaAI yAC'GTr

Cuts at:      0    1350    3243    4743 
   Size:       1350    1893    1500

  Fragments arranged by size: 

                1893    1500    1350
 
BsaBI GATnn'nnATC

Cuts at:      0    1376    2180    4700    4743 
   Size:       1376     804    2520      43

  Fragments arranged by size: 

                2520    1376     804      43
 
BsaHI Gr'CG_yC

Cuts at:      0    1290    4743 
   Size:       1290    3453
 
BsaWI w'CCGG_w

Cuts at:      0    1678    3052    4119    4743 
   Size:       1678    1374    1067     624

  Fragments arranged by size: 

                1678    1374    1067     624
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     589     619    4743 
   Size:        589      30    4124

  Fragments arranged by size: 

                4124     589      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     610     997    2558    4506    4627    4705    4714    4743 
   Size:        610     387    1561    1948     121      78       9      29

  Fragments arranged by size: 

                1948    1561     610     387     121      78      29       9
            
 
BseYI C'CCAG_C

Cuts at:      0    1121    3038    4677    4743 
   Size:       1121    1917    1639      66

  Fragments arranged by size: 

                1917    1639    1121      66
 
BsiEI CG_ry'CG

Cuts at:      0    4217    4743 
   Size:       4217     526
 
BsiHKAI G_wGCw'C

Cuts at:      0     452    2681    3033    4661    4743 
   Size:        452    2229     352    1628      82

  Fragments arranged by size: 

                2229    1628     452     352      82
 
BsmI GAATG_Cn'

Cuts at:      0     437     633    4743 
   Size:        437     196    4110

  Fragments arranged by size: 

                4110     437     196
 
Bsp1286I G_dGCh'C

Cuts at:      0     452    1972    2681    3033    4293    4460    4661    4743 
   Size:        452    1520     709     352    1260     167     201      82

  Fragments arranged by size: 

                1520    1260     709     452     352     201     167      82
            
 
BspEI T'CCGG_A

Cuts at:      0    3052    4743 
   Size:       3052    1691
 
BspHI T'CATG_A

Cuts at:      0    1389    3233    4743 
   Size:       1389    1844    1510

  Fragments arranged by size: 

                1844    1510    1389
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     289    3399    4427    4635    4743 
   Size:        289    3110    1028     208     108

  Fragments arranged by size: 

                3110    1028     289     208     108
 
BsrBI CCG'CTC

Cuts at:      0    3935    4743 
   Size:       3935     808
 
BsrDI GCAATG_nn'

Cuts at:      0     109    2279    2808    2993    3719    4743 
   Size:        109    2170     529     185     726    1024

  Fragments arranged by size: 

                2170    1024     726     529     185     109
 
BsrFI r'CCGG_y

Cuts at:      0     988    4743 
   Size:        988    3755
 
BsrGI T'GTAC_A

Cuts at:      0    1046    3468    4743 
   Size:       1046    2422    1275

  Fragments arranged by size: 

                2422    1275    1046
 
BssSI C'ACGA_G

Cuts at:      0    3795    4743 
   Size:       3795     948
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2803    4743 
   Size:       2803    1940
 
BstBI TT'CG_AA

Cuts at:      0    3158    3350    4743 
   Size:       3158     192    1393

  Fragments arranged by size: 

                3158    1393     192
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     189    4743 
   Size:        189    4554
 
BstYI r'GATC_y

Cuts at:      0     865    1500    1821    2169    3573    4472    4743 
   Size:        865     635     321     348    1404     899     271

  Fragments arranged by size: 

                1404     899     865     635     348     321     271
 
BstZ17I GTA'TAC

Cuts at:      0     234    2522    4743 
   Size:        234    2288    2221

  Fragments arranged by size: 

                2288    2221     234
 
Bsu36I CC'TnA_GG

Cuts at:      0    3974    4477    4743 
   Size:       3974     503     266

  Fragments arranged by size: 

                3974     503     266
 
BtgI C'CryG_G

Cuts at:      0     182    2525    4743 
   Size:        182    2343    2218

  Fragments arranged by size: 

                2343    2218     182
 
BtsI GCAGTG_nn'

Cuts at:      0     172    1532    1570    2473    4743 
   Size:        172    1360      38     903    2270

  Fragments arranged by size: 

                2270    1360     903     172      38
 
ClaI AT'CG_AT

Cuts at:      0    3011    4743 
   Size:       3011    1732
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3384    3419    4743 
   Size:       3384      35    1324

  Fragments arranged by size: 

                3384    1324      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     588    2762    4743 
   Size:        588    2174    1981

  Fragments arranged by size: 

                2174    1981     588
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1289    4743 
   Size:       1289    3454
 
EaeI y'GGCC_r

Cuts at:      0    4343    4743 
   Size:       4343     400
 
EarI CTCTTCn'nnn_

Cuts at:      0       1     542    1891    1977    2361    2513    3533    3748 
   Size:          1     541    1349      86     384     152    1020     215

Cuts at:   3748    4730    4743 
   Size:        982      13

  Fragments arranged by size: 

                1349    1020     982     541     384     215     152      86
                  13       1
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1939    4743 
   Size:       1939    2804
 
EcoICRI GAG'CTC

Cuts at:      0    3031    4659    4743 
   Size:       3031    1628      84

  Fragments arranged by size: 

                3031    1628      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     891    1330    1397    1872    2263    3473    3911 
   Size:        383     508     439      67     475     391    1210     438

Cuts at:   3911    3973    4039    4132    4444    4660    4675    4743 
   Size:         62      66      93     312     216      15      68

  Fragments arranged by size: 

                1210     508     475     439     438     391     383     312
                 216      93      68      67      66      62      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2189    4642    4729    4743 
   Size:       2189    2453      87      14

  Fragments arranged by size: 

                2453    2189      87      14
 
EcoRI G'AATT_C

Cuts at:      0     105     343    1149    1417    3687    4743 
   Size:        105     238     806     268    2270    1056

  Fragments arranged by size: 

                2270    1056     806     268     238     105
 
EcoRV GAT'ATC

Cuts at:      0    2008    4743 
   Size:       2008    2735
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2131    4176    4743 
   Size:       2131    2045     567

  Fragments arranged by size: 

                2131    2045     567
 
FspI TGC'GCA

Cuts at:      0    2418    4743 
   Size:       2418    2325
 
HaeII r_GCGC'y

Cuts at:      0    1411    3091    4743 
   Size:       1411    1680    1652

  Fragments arranged by size: 

                1680    1652    1411
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     589     621    1992    2024    2396    2428    2722    2754 
   Size:        589      32    1371      32     372      32     294      32

Cuts at:   2754    3558    3590    4195    4227    4331    4363    4743 
   Size:        804      32     605      32     104      32     380

  Fragments arranged by size: 

                1371     804     605     589     380     372     294     104
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0      28    1568    4214    4743 
   Size:         28    1540    2646     529

  Fragments arranged by size: 

                2646    1540     529      28
 
HindIII A'AGCT_T

Cuts at:      0    1083    1636    2016    4568    4743 
   Size:       1083     553     380    2552     175

  Fragments arranged by size: 

                2552    1083     553     380     175
 
MfeI C'AATT_G

Cuts at:      0    2420    4743 
   Size:       2420    2323
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1778    4410    4743 
   Size:       1778    2632     333

  Fragments arranged by size: 

                2632    1778     333
 
MscI TGG'CCA

Cuts at:      0    4345    4743 
   Size:       4345     398
 
MslI CAynn'nnrTG

Cuts at:      0     473    2758    2998    3232    3880    4743 
   Size:        473    2285     240     234     648     863

  Fragments arranged by size: 

                2285     863     648     473     240     234
 
MspA1I CmG'CkG

Cuts at:      0    3728    4110    4743 
   Size:       3728     382     633

  Fragments arranged by size: 

                3728     633     382
 
NcoI C'CATG_G

Cuts at:      0     182    2525    4743 
   Size:        182    2343    2218

  Fragments arranged by size: 

                2343    2218     182
 
NsiI A_TGCA'T

Cuts at:      0     635     720    1161    2709    4743 
   Size:        635      85     441    1548    2034

  Fragments arranged by size: 

                2034    1548     635     441      85
 
NspI r_CATG'y

Cuts at:      0    2801    3168    4743 
   Size:       2801     367    1575

  Fragments arranged by size: 

                2801    1575     367
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1813    1902    3910    4311    4743 
   Size:       1813      89    2008     401     432

  Fragments arranged by size: 

                2008    1813     432     401      89
 
PfoI T'CCnGG_A

Cuts at:      0    2589    4652    4743 
   Size:       2589    2063      91

  Fragments arranged by size: 

                2589    2063      91
 
PmlI CAC'GTG

Cuts at:      0    1350    4743 
   Size:       1350    3393
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2336    2368    4743 
   Size:       2336      32    2375

  Fragments arranged by size: 

                2375    2336      32
 
PsiI TTA'TAA

Cuts at:      0     661     862    2744    3020    4532    4743 
   Size:        661     201    1882     276    1512     211

  Fragments arranged by size: 

                1882    1512     661     276     211     201
 
PstI C_TGCA'G

Cuts at:      0    4103    4743 
   Size:       4103     640
 
PvuII CAG'CTG

Cuts at:      0    3728    4110    4743 
   Size:       3728     382     633

  Fragments arranged by size: 

                3728     633     382
 
SacI G_AGCT'C

Cuts at:      0    3033    4661    4743 
   Size:       3033    1628      82

  Fragments arranged by size: 

                3033    1628      82
 
SalI G'TCGA_C

Cuts at:      0    4212    4743 
   Size:       4212     531
 
SapI GCTCTTCn'nnn_

Cuts at:      0     542    1977    4730    4743 
   Size:        542    1435    2753      13

  Fragments arranged by size: 

                2753    1435     542      13
 
SfcI C'TryA_G

Cuts at:      0    4099    4743 
   Size:       4099     644
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    4261    4743 
   Size:       4261     482
 
SmlI C'TyrA_G

Cuts at:      0    2013    4093    4743 
   Size:       2013    2080     650

  Fragments arranged by size: 

                2080    2013     650
 
SphI G_CATG'C

Cuts at:      0    2801    4743 
   Size:       2801    1942
 
StuI AGG'CCT

Cuts at:      0     757    4743 
   Size:        757    3986
 
StyI C'CwwG_G

Cuts at:      0     182    1067    1422    1947    2190    2525    4664    4743 
   Size:        182     885     355     525     243     335    2139      79

  Fragments arranged by size: 

                2139     885     525     355     335     243     182      79
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3023    4318    4352    4669    4743 
   Size:       3023    1295      34     317      74

  Fragments arranged by size: 

                3023    1295     317      74      34
 
TatI w'GTAC_w

Cuts at:      0    1046    2298    2880    2902    3107    3468    4616    4743 
   Size:       1046    1252     582      22     205     361    1148     127

  Fragments arranged by size: 

                1252    1148    1046     582     361     205     127      22
            
 
Tth111I GACn'n_nGTC

Cuts at:      0    4131    4743 
   Size:       4131     612
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4308    4743 
   Size:       4308     435
 
XmnI GAAnn'nnTTC

Cuts at:      0     979    2821    3650    4743 
   Size:        979    1842     829    1093

  Fragments arranged by size: 

                1842    1093     979     829
 
ZraI GAC'GTC

Cuts at:      0    1291    4743 
   Size:       1291    3452
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI    AflII     AloI    AlwNI     ApoI
     AseI     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BbvCI
     BclI    BfrBI     BglI    BglII Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI
    PflMI     PfoI     PmlI     PpiI     PsiI     PstI    PvuII     SacI
     SalI     SapI     SfcI     SfiI     SmlI     SphI     StuI     StyI
    TaqII     TatI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AleI     AlfI
     ApaI    ApaLI     AscI    AsiSI     BaeI     BbeI     BcgI    BciVI
     BlpI    BmgBI     BmtI     BsgI    BsiWI    BsmBI   BssHII   BstEII
    BtgZI     DraI     EagI EcoO109I     FseI    FspAI     HpaI     KasI
     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI     PmeI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII    SanDI     SbfI     ScaI
    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI     TstI     XbaI     XhoI     XmaI
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