Restriction map for pF1KB6271
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AhdI        ApoI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BamHI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BsaBI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrDI       BsrGI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KpnI        MamI        MfeI        MluI        MroI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        SexAI       SfuI        
SmaI        SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        Tru9I       Tsp509I     
Tth111I     XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          366  GTCTAC               GT^[AC][GT]AC
AgeI         1517  ACCGGT               A^CCGGT
AocI          765  CCTGAGG              CC^T[ACGT]AGG
AscI          684  GGCGCGCC             GG^CGCGCC
AviII         820  TGCGCA               TGC^GCA
BalI         1343  TGGCCA               TGG^CCA
BbrPI        1878  CACGTG               CAC^GTG
BbsI         1138  GTCTTC               GAAGAC(2/6)
BpuAI        1138  GTCTTC               GAAGAC(2/6)
BsaI          346  GAGACC               GGTCTC(1/5)
BsmBI         202  GAGACG               CGTCTC(1/5)
BspHI         836  TCATGA               T^CATGA
BssSI        1129  CACGAG               CACGAG(-5/-1)
BstAPI        456  GCAGGCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI          80  AGATCC               [GA]^GATC[CT]
Bsu36I        765  CCTGAGG              CC^T[ACGT]AGG
DrdI         1588  GACTCGCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        765  CCTGAGG              CC^T[ACGT]AGG
FspI          820  TGCGCA               TGC^GCA
HincII        229  GTCAAC               GT[CT]^[GA]AC
HindII        229  GTCAAC               GT[CT]^[GA]AC
MflI           80  AGATCC               [GA]^GATC[CT]
MluNI        1343  TGGCCA               TGG^CCA
MscI         1343  TGGCCA               TGG^CCA
NspI         1032  GCATGT               [GA]CATG^[CT]
PflMI        1479  CCATCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1517  ACCGGT               A^CCGGT
PmaCI        1878  CACGTG               CAC^GTG
PmlI         1878  CACGTG               CAC^GTG
PpuMI         602  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI          836  TCATGA               T^CATGA
SapI          570  GCTCTTC              GCTCTTC(1/4)
SbfI         1752  CCTGCAGG             CCTGCA^GG
ScaI         1046  AGTACT               AGT^ACT
SfiI          744  GGCCGCTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI          33  CGCCGGCG             C[GA]^CCGG[CT]G
Sse8387I     1752  CCTGCAGG             CCTGCA^GG
StuI          731  AGGCCT               AGG^CCT
TfiI          117  GATTC                G^A[AT]TC
Van91I       1479  CCATCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1780  TCTAGA               T^CTAGA
XhoII          80  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Alw44I        394  GTGCAC               G^TGCAC
              967  GTGCAC              
Aor51HI       805  AGCGCT               AGC^GCT
             1334  AGCGCT              
ApaLI         394  GTGCAC               G^TGCAC
              967  GTGCAC              
BfaI          964  CTAG                 C^TAG
             1781  CTAG                
BlpI          219  GCTGAGC              GC^T[ACGT]AGC
              270  GCTCAGC             
Bpu1102I      219  GCTGAGC              GC^T[ACGT]AGC
              270  GCTCAGC             
BsaWI         971  ACCGGA               [AT]^CCGG[AT]
             1517  ACCGGT              
BsgI          702  CTGCAC               GTGCAG(16/14)
             1267  CTGCAC              
BsrBI         696  CCGCTC               CCGCTC(-3/-3)
             1053  CCGCTC              
BssHII        538  GCGCGC               G^CGCGC
              685  GCGCGC              
BstXI         350  CCAAGGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1684  CCATGCAGGTGG        
CelII         219  GCTGAGC              GC^T[ACGT]AGC
              270  GCTCAGC             
EarI          571  CTCTTC               CTCTTC(1/4)
              649  CTCTTC              
Eco47III      805  AGCGCT               AGC^GCT
             1334  AGCGCT              
Ksp632I       571  CTCTTC               CTCTTC(1/4)
              649  CTCTTC              
KspI           49  CCGCGG               CCGC^GG
             1631  CCGCGG              
MaeI          964  CTAG                 C^TAG
             1781  CTAG                
MslI         1685  CATGCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1874  CATCCACGTG          
SacII          49  CCGCGG               CCGC^GG
             1631  CCGCGG              
TaqI          632  TCGA                 T^CGA
             1361  TCGA                
TthHB8I       632  TCGA                 T^CGA
             1361  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbeI        BbvI        
BcnI        BglI        BmyI        BpmI        BsaAI       
BsaHI       BsaJI       BseRI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        MaeII       MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       PleI        
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
StyI        TseI        Tsp45I      TspRI       XcmI        
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