Restriction map for pF1KB6710
 (Circular) (Six-base) MAPSORT of: pF1KB6710.seq  Check: 5327  from: 1  to: 3503



pF1KB6710  3503 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AccI GT'mk_AC

Cuts at:    541     541 
   Size:       3503
 
Acc65I G'GTAC_C

Cuts at:    519     519 
   Size:       3503
 
AclI AA'CG_TT

Cuts at:   3343    3343 
   Size:       3503
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1328    1760    2507    2775    1328 
   Size:        432     747     268    2056

  Fragments arranged by size: 

                2056     747     432     268
 
AflIII A'CryG_T

Cuts at:    855    1959    2936     855 
   Size:       1104     977    1422

  Fragments arranged by size: 

                1422    1104     977
 
AgeI A'CCGG_T

Cuts at:   1891    1891 
   Size:       3503
 
AlwNI CAG_nnn'CTG

Cuts at:    808    2375     808 
   Size:       1567    1936
 
ApaLI G'TGCA_C

Cuts at:   2273    2273 
   Size:       3503
 
ApoI r'AATT_y

Cuts at:    253     507    2856    3112     253 
   Size:        254    2349     256     644

  Fragments arranged by size: 

                2349     644     256     254
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3503
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3503
 
AvaI C'yCGr_G

Cuts at:    523     523 
   Size:       3503
 
BamHI G'GATC_C

Cuts at:    528     528 
   Size:       3503
 
BanI G'GyrC_C

Cuts at:    519     933    1181    1216     519 
   Size:        414     248      35    2806

  Fragments arranged by size: 

                2806     414     248      35
 
BanII G_rGCy'C

Cuts at:    517    1547     517 
   Size:       1030    2473
 
BbeI G_GCGC'C

Cuts at:    937    1185     937 
   Size:        248    3255
 
BbvCI CC'TCA_GC

Cuts at:    426     426 
   Size:       3503
 
BciVI GTATCCnnnnn_n'

Cuts at:   1401    2162    3503    1401 
   Size:        761    1341    1401

  Fragments arranged by size: 

                1401    1341     761
 
BclI T'GATC_A

Cuts at:    329    1023     329 
   Size:        694    2809
 
BglII A'GATC_T

Cuts at:   1018    3055    1018 
   Size:       2037    1466
 
BlpI GC'TnA_GC

Cuts at:    615     615 
   Size:       3503
 
Bme1580I G_kGCm'C

Cuts at:   1128    1221    2277    1128 
   Size:         93    1056    2354

  Fragments arranged by size: 

                2354    1056      93
 
BmgBI CAC'GTC

Cuts at:    348     348 
   Size:       3503
 
BmrI ACTGGGnnnn_n'

Cuts at:    715     796    1131    3275     715 
   Size:         81     335    2144     943

  Fragments arranged by size: 

                2144     943     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1870    1870 
   Size:       3503
 
Bpu10I CC'TnA_GC

Cuts at:    426     426 
   Size:       3503
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    124     679    1558    2050    2348     124 
   Size:        555     879     492     298    1279

  Fragments arranged by size: 

                1279     879     555     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3207    3207 
   Size:       3503
 
BsaAI yAC'GTr

Cuts at:    856     856 
   Size:       3503
 
BsaBI GATnn'nnATC

Cuts at:      6    1017    3054       6 
   Size:       1011    2037     455

  Fragments arranged by size: 

                2037    1011     455
 
BsaHI Gr'CG_yC

Cuts at:    934    1182    1884    3380     934 
   Size:        248     702    1496    1057

  Fragments arranged by size: 

                1496    1057     702     248
 
BsaWI w'CCGG_w

Cuts at:    212     692    1213    1891    2165    2312    2723     212 
   Size:        480     521     678     274     147     411     992

  Fragments arranged by size: 

                 992     678     521     480     411     274     147
 
BseYI C'CCAG_C

Cuts at:    280     928    1686    2263    2759     280 
   Size:        648     758     577     496    1024

  Fragments arranged by size: 

                1024     758     648     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1045    1091    2299     104 
   Size:        941      46    1208    1308

  Fragments arranged by size: 

                1308    1208     941      46
 
BsiHKAI G_wGCw'C

Cuts at:    199     517    1295    1485    2277     199 
   Size:        318     778     190     792    1425

  Fragments arranged by size: 

                1425     792     778     318     190
 
Bsp1286I G_dGCh'C

Cuts at:    199     517    1128    1221    1295    1485    1547    2277     199 
   Size:        318     611      93      74     190      62     730    1425

  Fragments arranged by size: 

                1425     730     611     318     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2723    2723 
   Size:       3503
 
BspHI T'CATG_A

Cuts at:   2679    2679 
   Size:       3503
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    553     767    1069    1450     553 
   Size:        214     302     381    2606

  Fragments arranged by size: 

                2606     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1795    1849    3333    3498      50 
   Size:       1745      54    1484     165      55

  Fragments arranged by size: 

                1745    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1415    1415 
   Size:       3503
 
BsrFI r'CCGG_y

Cuts at:   1501    1682    1891    1501 
   Size:        181     209    3113

  Fragments arranged by size: 

                3113     209     181
 
BssSI C'ACGA_G

Cuts at:   1774    2132    1774 
   Size:        358    3145
 
BstAPI GCAn_nnn'nTGC

Cuts at:    880     880 
   Size:       3503
 
BstBI TT'CG_AA

Cuts at:   1864    1864 
   Size:       3503
 
BstXI CCAn_nnnn'nTGG

Cuts at:    280     280 
   Size:       3503
 
BstYI r'GATC_y

Cuts at:      1     528     995    1018    1353    1599    2611    2697    2709 
   Size:        527     467      23     335     246    1012      86      12

Cuts at:   2709    3055       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     527     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3503
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1453    1634    1797    1453 
   Size:        181     163    3159

  Fragments arranged by size: 

                3159     181     163
 
BtsI GCAGTG_nn'

Cuts at:    746     746 
   Size:       3503
 
DraI TTT'AAA

Cuts at:    502     502 
   Size:       3503
 
DrdI GACnn_nn'nnGTC

Cuts at:   1209    2067    1209 
   Size:        858    2645
 
EaeI y'GGCC_r

Cuts at:   1088    1262    1653    1680    3025    1088 
   Size:        174     391      27    1345    1566

  Fragments arranged by size: 

                1566    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1088    1088 
   Size:       3503
 
EarI CTCTTCn'nnn_

Cuts at:   1526    1736    1526 
   Size:        210    3293
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1711    2021    2167    3313    1711 
   Size:        310     146    1146    1901

  Fragments arranged by size: 

                1901    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    515     515 
   Size:       3503
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1328    1760    2507    2775    1328 
   Size:        432     747     268    2056

  Fragments arranged by size: 

                2056     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    642     642 
   Size:       3503
 
EcoRI G'AATT_C

Cuts at:    507     507 
   Size:       3503
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1522    1522 
   Size:       3503
 
FspI TGC'GCA

Cuts at:    115    1284     115 
   Size:       1169    2334
 
FspAI rTGC'GCAy

Cuts at:    115     115 
   Size:       3503
 
HaeII r_GCGC'y

Cuts at:    937    1185    2207    3186     937 
   Size:        248    1022     979    1254

  Fragments arranged by size: 

                1254    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    464     496    1345    1375    1377    1407     464 
   Size:         32     849      30       2      30    2560

  Fragments arranged by size: 

                2560     849      32      30      30       2
 
HincII GTy'rAC

Cuts at:    542     542 
   Size:       3503
 
KasI G'GCGC_C

Cuts at:    933    1181     933 
   Size:        248    3255
 
KpnI G_GTAC'C

Cuts at:    523     523 
   Size:       3503
 
MluI A'CGCG_T

Cuts at:   2936    2936 
   Size:       3503
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2174    2358    2174 
   Size:        184    3319
 
MscI TGG'CCA

Cuts at:   1264    1264 
   Size:       3503
 
MslI CAynn'nnrTG

Cuts at:    226    1492    1619     226 
   Size:       1266     127    2110

  Fragments arranged by size: 

                2110    1266     127
 
MspA1I CmG'CkG

Cuts at:    430     614     928    1288    2301    2546     430 
   Size:        184     314     360    1013     245    1387

  Fragments arranged by size: 

                1387    1013     360     314     245     184
 
NaeI GCC'GGC

Cuts at:   1684    1684 
   Size:       3503
 
NarI GG'CG_CC

Cuts at:    934    1182     934 
   Size:        248    3255
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3503
 
NgoMIV G'CCGG_C

Cuts at:   1682    1682 
   Size:       3503
 
NspI r_CATG'y

Cuts at:    556    1056    1963     556 
   Size:        500     907    2096

  Fragments arranged by size: 

                2096     907     500
 
PciI A'CATG_T

Cuts at:   1959    1959 
   Size:       3503
 
PmeI GTTT'AAAC

Cuts at:    502     502 
   Size:       3503
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2642    2674    2642 
   Size:         32    3471
 
PstI C_TGCA'G

Cuts at:    550    1235     550 
   Size:        685    2818
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3503
 
PvuII CAG'CTG

Cuts at:    430     928    1288     430 
   Size:        498     360    2645

  Fragments arranged by size: 

                2645     498     360
 
RsrII CG'GwC_CG

Cuts at:   1698    1698 
   Size:       3503
 
SacI G_AGCT'C

Cuts at:    517     517 
   Size:       3503
 
SalI G'TCGA_C

Cuts at:    540     540 
   Size:       3503
 
SapI GCTCTTCn'nnn_

Cuts at:   1526    1736    1526 
   Size:        210    3293
 
SbfI CC_TGCA'GG

Cuts at:    550     550 
   Size:       3503
 
ScaI AGT'ACT

Cuts at:   3020    3020 
   Size:       3503
 
SfcI C'TryA_G

Cuts at:     33     546    1231    2224    2415      33 
   Size:        513     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     513     191
 
SfoI GGC'GCC

Cuts at:    935    1183     935 
   Size:        248    3255
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3503
 
SmaI CCC'GGG

Cuts at:    525     525 
   Size:       3503
 
SmlI C'TyrA_G

Cuts at:    139     658    1573    2065    2327     139 
   Size:        519     915     492     262    1315

  Fragments arranged by size: 

                1315     915     519     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3503
 
SphI G_CATG'C

Cuts at:    556    1056     556 
   Size:        500    3003
 
StyI C'CwwG_G

Cuts at:    107     637     991     107 
   Size:        530     354    2619

  Fragments arranged by size: 

                2619     530     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1086    1219    1678    1887    1086 
   Size:        133     459     209    2702

  Fragments arranged by size: 

                2702     459     209     133
 
TatI w'GTAC_w

Cuts at:    414    3018     414 
   Size:       2604     899
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3020    3052    3020 
   Size:         32    3471
 
Tth111I GACn'n_nGTC

Cuts at:   1300    1300 
   Size:       3503
 
XbaI T'CTAG_A

Cuts at:    534     534 
   Size:       3503
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    998     998 
   Size:       3503
 
XmaI C'CCGG_G

Cuts at:    523     523 
   Size:       3503
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PmeI
     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI     BglI
     BmtI     BpmI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI     ZraI
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