Restriction map for pF1KB9980
 (Linear) (Six-base) MAPSORT of: KIBB9980.seq  Check: 1538  from: 1  to: 2124



KIBB9980.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1247    2124 
   Size:       1247     877
 
AflIII A'CryG_T

Cuts at:      0     846    2124 
   Size:        846    1278
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1789    2124 
   Size:       1789     335
 
ApoI r'AATT_y

Cuts at:      0     125     275     893    1150    1378    1746    2124 
   Size:        125     150     618     257     228     368     378

  Fragments arranged by size: 

                 618     378     368     257     228     150     125
 
AvaI C'yCGr_G

Cuts at:      0     226    2124 
   Size:        226    1898
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      95     128    2124 
   Size:         95      33    1996

  Fragments arranged by size: 

                1996      95      33
 
BamHI G'GATC_C

Cuts at:      0     544    2124 
   Size:        544    1580
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1487    1964    2124 
   Size:       1487     477     160

  Fragments arranged by size: 

                1487     477     160
 
BclI T'GATC_A

Cuts at:      0     285    2124 
   Size:        285    1839
 
BfrBI ATG'CAT

Cuts at:      0     888    2124 
   Size:        888    1236
 
BglII A'GATC_T

Cuts at:      0    1290    1588    2124 
   Size:       1290     298     536

  Fragments arranged by size: 

                1290     536     298
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1816    2124 
   Size:       1816     308
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     186    2124 
   Size:        186    1938
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     597    1772    2124 
   Size:        597    1175     352

  Fragments arranged by size: 

                1175     597     352
 
BsaBI GATnn'nnATC

Cuts at:      0      62    2081    2124 
   Size:         62    2019      43

  Fragments arranged by size: 

                2019      62      43
 
BsaWI w'CCGG_w

Cuts at:      0     495    2124 
   Size:        495    1629
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     821     851    2124 
   Size:        821      30    1273

  Fragments arranged by size: 

                1273     821      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1408    1510    1615    1775    2124 
   Size:       1408     102     105     160     349

  Fragments arranged by size: 

                1408     349     160     105     102
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1351    2124 
   Size:       1351     773
 
BsiHKAI G_wGCw'C

Cuts at:      0    1584    2124 
   Size:       1584     540
 
Bsp1286I G_dGCh'C

Cuts at:      0    1584    2124 
   Size:       1584     540
 
BspHI T'CATG_A

Cuts at:      0     186     384    2124 
   Size:        186     198    1740

  Fragments arranged by size: 

                1740     198     186
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     229    2124 
   Size:        229    1895
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     415    2124 
   Size:        415    1709
 
BstYI r'GATC_y

Cuts at:      0     544    1290    1588    2124 
   Size:        544     746     298     536

  Fragments arranged by size: 

                 746     544     536     298
 
BtgI C'CryG_G

Cuts at:      0     319    2124 
   Size:        319    1805
 
BtsI GCAGTG_nn'

Cuts at:      0    1339    2124 
   Size:       1339     785
 
ClaI AT'CG_AT

Cuts at:      0    1490    2124 
   Size:       1490     634
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     455     490    2124 
   Size:        455      35    1634

  Fragments arranged by size: 

                1634     455      35
 
DraI TTT'AAA

Cuts at:      0      39    1872    2124 
   Size:         39    1833     252

  Fragments arranged by size: 

                1833     252      39
 
DraIII CAC_nnn'GTG

Cuts at:      0     415     909    2124 
   Size:        415     494    1215

  Fragments arranged by size: 

                1215     494     415
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1833    2124 
   Size:       1833     291
 
EarI CTCTTCn'nnn_

Cuts at:      0     905    1223    1701    1713    1722    1731    1773    2124 
   Size:        905     318     478      12       9       9      42     351

  Fragments arranged by size: 

                 905     478     351     318      42      12       9       9
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1247    1816    2124 
   Size:       1247     569     308

  Fragments arranged by size: 

                1247     569     308
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1265    2124 
   Size:       1265     859
 
EcoRI G'AATT_C

Cuts at:      0    1746    2124 
   Size:       1746     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1045    2124 
   Size:       1045    1079
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     607     639    1217    1249    1669    1701    2044    2076 
   Size:        607      32     578      32     420      32     343      32

Cuts at:   2076    2124 
   Size:         48

  Fragments arranged by size: 

                 607     578     420     343      48      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     297    2124 
   Size:        297    1827
 
HindIII A'AGCT_T

Cuts at:      0     999    2124 
   Size:        999    1125
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     781    1412    2124 
   Size:        781     631     712

  Fragments arranged by size: 

                 781     712     631
 
MslI CAynn'nnrTG

Cuts at:      0     737    1469    2124 
   Size:        737     732     655

  Fragments arranged by size: 

                 737     732     655
 
MspA1I CmG'CkG

Cuts at:      0     777    2124 
   Size:        777    1347
 
NcoI C'CATG_G

Cuts at:      0     319    2124 
   Size:        319    1805
 
NsiI A_TGCA'T

Cuts at:      0     890    2124 
   Size:        890    1234
 
NspI r_CATG'y

Cuts at:      0     850    2124 
   Size:        850    1274
 
PciI A'CATG_T

Cuts at:      0     846    2124 
   Size:        846    1278
 
PshAI GACnn'nnGTC

Cuts at:      0     491    1790    2124 
   Size:        491    1299     334

  Fragments arranged by size: 

                1299     491     334
 
PspXI vC'TCGA_Gb

Cuts at:      0     226    2124 
   Size:        226    1898
 
PstI C_TGCA'G

Cuts at:      0      12    2124 
   Size:         12    2112
 
PvuII CAG'CTG

Cuts at:      0     777    2124 
   Size:        777    1347
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1773    2124 
   Size:       1773     351
 
ScaI AGT'ACT

Cuts at:      0       6     675    1306    2124 
   Size:          6     669     631     818

  Fragments arranged by size: 

                 818     669     631       6
 
SfcI C'TryA_G

Cuts at:      0       8    1104    2124 
   Size:          8    1096    1020

  Fragments arranged by size: 

                1096    1020       8
 
SmlI C'TyrA_G

Cuts at:      0     201     226    2124 
   Size:        201      25    1898

  Fragments arranged by size: 

                1898     201      25
 
SpeI A'CTAG_T

Cuts at:      0    2098    2124 
   Size:       2098      26
 
SspI AAT'ATT

Cuts at:      0      44     244    2124 
   Size:         44     200    1880

  Fragments arranged by size: 

                1880     200      44
 
StyI C'CwwG_G

Cuts at:      0     319     419    2124 
   Size:        319     100    1705

  Fragments arranged by size: 

                1705     319     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     241     312    1351    2124 
   Size:        241      71    1039     773

  Fragments arranged by size: 

                1039     773     241      71
 
TatI w'GTAC_w

Cuts at:      0       4     673    1304    1371    2124 
   Size:          4     669     631      67     753

  Fragments arranged by size: 

                 753     669     631      67       4
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     791     823    2124 
   Size:        791      32    1301

  Fragments arranged by size: 

                1301     791      32
 
XbaI T'CTAG_A

Cuts at:      0    1843    2124 
   Size:       1843     281
 
XhoI C'TCGA_G

Cuts at:      0     226    2124 
   Size:        226    1898
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     AvaI     BaeI    BamHI     BbsI
     BclI    BfrBI    BglII     BpmI    BpuEI     BsaI    BsaBI    BsaWI
    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI   BstAPI
    BstYI     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EarI  Eco57MI    EcoNI    EcoRI     FalI    Hin4I   HincII  HindIII
     MmeI     MslI   MspA1I     NcoI     NsiI     NspI     PciI    PshAI
    PspXI     PstI    PvuII     SapI     ScaI     SfcI     SmlI     SpeI
     SspI     StyI    TaqII     TatI     TstI     XbaI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BsaAI    BsaHI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     EaeI     EagI     EciI  EcoICRI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StuI     SwaI
  Tth111I     XcmI     XmaI     XmnI     ZraI
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