Restriction map for pF1KB5894
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AvaI        AviII       BamHI       
BanI        BbeI        BbrPI       BbsI        BcgI        
BcnI        BfrI        BglI        BglII       BpuAI       
BsaAI       BsaHI       BsaI        BseRI       BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspHI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FauI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MflI        MluI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XbaI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccIII        949  TCCGGA               T^CCGGA
AflIII        129  ACATGT               A^C[GA][CT]GT
AlwI         1251  GATCC                GGATC(4/5)
AseI          817  ATTAAT               AT^TAAT
AsnI          817  ATTAAT               AT^TAAT
Asp700I      1170  GAATAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1289  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1551  CCTAGG               C^CTAGG
BalI          581  TGGCCA               TGG^CCA
BanII         413  GAGCTC               G[GA]GC[CT]^C
BbvCI         244  GCTGAGG              CCTCAGC(-5/-2)
BciVI         301  GTATCC               GTATCC(6/5)
BclI           89  TGATCA               T^GATCA
BlnI         1551  CCTAGG               C^CTAGG
BpmI         1409  CTGGAG               CTGGAG(16/14)
BsaBI        1203  GATAGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         949  TCCGGA               [AT]^CCGG[AT]
BseAI         949  TCCGGA               T^CCGGA
BspEI         949  TCCGGA               T^CCGGA
BspLU11I      129  ACATGT               A^CATGT
BspMI        1333  ACCTGC               ACCTGC(4/8)
BsrBI         545  CCGCTC               CCGCTC(-3/-3)
BsrDI        1368  CATTGC               GCAATG(2/0)
BstXI         232  CCAACCCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1037  GCGC                 GCG^C
DraI         1444  TTTAAA               TTT^AAA
DraII          22  GGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI          581  TGGCCA               [CT]^GGCC[GA]
EcoO109I       22  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1492  GAATTC               G^AATTC
FbaI           89  TGATCA               T^GATCA
HaeII        1036  GGCGCT               [GA]GCGC^[CT]
HapII         950  CCGG                 C^CGG
HhaI         1037  GCGC                 GCG^C
HinP1I       1037  GCGC                 G^CGC
HindIII       774  AAGCTT               A^AGCTT
HpaII         950  CCGG                 C^CGG
MamI         1203  GATAGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1326  CAATTG               C^AATTG
MluNI         581  TGGCCA               TGG^CCA
MroI          949  TCCGGA               T^CCGGA
MscI          581  TGGCCA               TGG^CCA
MspI          950  CCGG                 C^CGG
MunI         1326  CAATTG               C^AATTG
PflFI        1289  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         281  CCATGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          785  GACTC                GAGTC(4/5)
PpuMI          22  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI         817  ATTAAT               AT^TAAT
PstI         1487  CTGCAG               CTGCA^G
SacI          413  GAGCTC               GAGCT^C
SapI          879  GCTCTTC              GCTCTTC(1/4)
StuI          507  AGGCCT               AGG^CCT
TaqI          850  TCGA                 T^CGA
Tth111I      1289  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       850  TCGA                 T^CGA
Van91I        281  CCATGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1497  CCAAAAGAAGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1170  GAATAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI          545  CCGC                 CCGC(-3/-1)
             1372  GCGG                
AfaI          188  GTAC                 GT^AC
             1365  GTAC                
AlwNI         694  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1562  CAGAGACTG           
BfaI          913  CTAG                 C^TAG
             1552  CTAG                
BlpI          937  GCTCAGC              GC^T[ACGT]AGC
             1511  GCTCAGC             
Bpu1102I      937  GCTCAGC              GC^T[ACGT]AGC
             1511  GCTCAGC             
BsiYI         281  CCATGCTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              545  CCGCTCTCAGG         
BslI          281  CCATGCTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              545  CCGCTCTCAGG         
BsmI          596  GCATTC               GAATGC(1/-1)
             1318  GCATTC              
BstAPI        654  GCACATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1309  GCAATAGCTGC         
Cac8I         579  GCTGGC               GC[ACGT]^[ACGT]GC
              681  GCGAGC              
CelII         937  GCTCAGC              GC^T[ACGT]AGC
             1511  GCTCAGC             
EcoT14I       238  CCTTGG               C^C[AT][AT]GG
             1551  CCTAGG              
MaeI          913  CTAG                 C^TAG
             1552  CTAG                
MaeII        1095  ACGT                 A^CGT
             1293  ACGT                
MseI          818  TTAA                 T^TAA
             1445  TTAA                
MspA1I        263  CAGCTG               C[AC]G^C[GT]G
              577  CAGCTG              
NspI          129  ACATGT               [GA]CATG^[CT]
              930  GCATGT              
PvuII         263  CAGCTG               CAG^CTG
              577  CAGCTG              
RsaI          188  GTAC                 GT^AC
             1365  GTAC                
StyI          238  CCTTGG               C^C[AT][AT]GG
             1551  CCTAGG              
TfiI          371  GAATC                G^A[AT]TC
              953  GAATC               
Tru9I         818  TTAA                 T^TAA
             1445  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AcsI        AluI        ApoI        AspHI       AvaII       
BbvI        BmyI        BsaJI       BsiHKAI     BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MslI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TseI        Tsp45I      
Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com