Restriction map for pF1KB5894
 (Linear) (Six-base) MAPSORT of: KIBB5894.seq  Check: 4228  from: 1  to: 1572



KIBB5894.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676    1572 
   Size:        676     896
 
AflIII A'CryG_T

Cuts at:      0     129    1572 
   Size:        129    1443
 
AleI CACnn'nnGTG

Cuts at:      0      49    1572 
   Size:         49    1523
 
AlwNI CAG_nnn'CTG

Cuts at:      0     699    1567    1572 
   Size:        699     868       5

  Fragments arranged by size: 

                 868     699       5
 
ApoI r'AATT_y

Cuts at:      0     625    1492    1546    1572 
   Size:        625     867      54      26

  Fragments arranged by size: 

                 867     625      54      26
 
AseI AT'TA_AT

Cuts at:      0     818    1572 
   Size:        818     754
 
AvrII C'CTAG_G

Cuts at:      0    1551    1572 
   Size:       1551      21
 
BanII G_rGCy'C

Cuts at:      0     417    1572 
   Size:        417    1155
 
BbvCI CC'TCA_GC

Cuts at:      0     245    1572 
   Size:        245    1327
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     312    1572 
   Size:        312    1260
 
BclI T'GATC_A

Cuts at:      0      89    1572 
   Size:         89    1483
 
BlpI GC'TnA_GC

Cuts at:      0     938    1512    1572 
   Size:        938     574      60

  Fragments arranged by size: 

                 938     574      60
 
BmgBI CAC'GTC

Cuts at:      0    1294    1572 
   Size:       1294     278
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      29    1572 
   Size:         29    1543
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1430    1572 
   Size:       1430     142
 
Bpu10I CC'TnA_GC

Cuts at:      0     245    1572 
   Size:        245    1327
 
BsaBI GATnn'nnATC

Cuts at:      0    1207    1572 
   Size:       1207     365
 
BsaWI w'CCGG_w

Cuts at:      0     949    1572 
   Size:        949     623
 
BseYI C'CCAG_C

Cuts at:      0      54    1572 
   Size:         54    1518
 
BsiHKAI G_wGCw'C

Cuts at:      0     417     561    1418    1572 
   Size:        417     144     857     154

  Fragments arranged by size: 

                 857     417     154     144
 
BsmI GAATG_Cn'

Cuts at:      0     596    1318    1572 
   Size:        596     722     254

  Fragments arranged by size: 

                 722     596     254
 
Bsp1286I G_dGCh'C

Cuts at:      0     417     561    1418    1572 
   Size:        417     144     857     154

  Fragments arranged by size: 

                 857     417     154     144
 
BspEI T'CCGG_A

Cuts at:      0     949    1572 
   Size:        949     623
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1342    1572 
   Size:       1342     230
 
BsrBI CCG'CTC

Cuts at:      0     547    1572 
   Size:        547    1025
 
BsrDI GCAATG_nn'

Cuts at:      0    1367    1572 
   Size:       1367     205
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     660    1315    1572 
   Size:        660     655     257

  Fragments arranged by size: 

                 660     655     257
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     239    1572 
   Size:        239    1333
 
BtsI GCAGTG_nn'

Cuts at:      0      44    1055    1572 
   Size:         44    1011     517

  Fragments arranged by size: 

                1011     517      44
 
DraI TTT'AAA

Cuts at:      0    1446    1572 
   Size:       1446     126
 
EaeI y'GGCC_r

Cuts at:      0     581    1572 
   Size:        581     991
 
EarI CTCTTCn'nnn_

Cuts at:      0     245     886    1498    1572 
   Size:        245     641     612      74

  Fragments arranged by size: 

                 641     612     245      74
 
EcoICRI GAG'CTC

Cuts at:      0     415    1572 
   Size:        415    1157
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676    1430    1572 
   Size:        676     754     142

  Fragments arranged by size: 

                 754     676     142
 
EcoO109I rG'GnC_Cy

Cuts at:      0      23    1572 
   Size:         23    1549
 
EcoRI G'AATT_C

Cuts at:      0    1492    1572 
   Size:       1492      80
 
HaeII r_GCGC'y

Cuts at:      0    1040    1572 
   Size:       1040     532
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     603     635    1085    1117    1572 
   Size:        603      32     450      32     455

  Fragments arranged by size: 

                 603     455     450      32      32
 
HindIII A'AGCT_T

Cuts at:      0     774    1572 
   Size:        774     798
 
MfeI C'AATT_G

Cuts at:      0    1326    1572 
   Size:       1326     246
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     390     565    1572 
   Size:        390     175    1007

  Fragments arranged by size: 

                1007     390     175
 
MscI TGG'CCA

Cuts at:      0     583    1572 
   Size:        583     989
 
MslI CAynn'nnrTG

Cuts at:      0      49      85    1144    1150    1572 
   Size:         49      36    1059       6     422

  Fragments arranged by size: 

                1059     422      49      36       6
 
MspA1I CmG'CkG

Cuts at:      0     265     579    1572 
   Size:        265     314     993

  Fragments arranged by size: 

                 993     314     265
 
NspI r_CATG'y

Cuts at:      0     133     934    1572 
   Size:        133     801     638

  Fragments arranged by size: 

                 801     638     133
 
PciI A'CATG_T

Cuts at:      0     129    1572 
   Size:        129    1443
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     287    1572 
   Size:        287    1285
 
PpuMI rG'GwC_Cy

Cuts at:      0      23    1572 
   Size:         23    1549
 
PsiI TTA'TAA

Cuts at:      0     460    1572 
   Size:        460    1112
 
PstI C_TGCA'G

Cuts at:      0    1491    1572 
   Size:       1491      81
 
PvuII CAG'CTG

Cuts at:      0     265     579    1572 
   Size:        265     314     993

  Fragments arranged by size: 

                 993     314     265
 
SacI G_AGCT'C

Cuts at:      0     417    1572 
   Size:        417    1155
 
SanDI GG'GwC_CC

Cuts at:      0      23    1572 
   Size:         23    1549
 
SapI GCTCTTCn'nnn_

Cuts at:      0     886    1572 
   Size:        886     686
 
SfcI C'TryA_G

Cuts at:      0     123    1487    1535    1559    1572 
   Size:        123    1364      48      24      13

  Fragments arranged by size: 

                1364     123      48      24      13
 
StuI AGG'CCT

Cuts at:      0     509    1572 
   Size:        509    1063
 
StyI C'CwwG_G

Cuts at:      0     238    1551    1572 
   Size:        238    1313      21

  Fragments arranged by size: 

                1313     238      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     127     170     335    1572 
   Size:        127      43     165    1237

  Fragments arranged by size: 

                1237     165     127      43
 
TatI w'GTAC_w

Cuts at:      0     187    1364    1572 
   Size:        187    1177     208

  Fragments arranged by size: 

                1177     208     187
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1376    1408    1572 
   Size:       1376      32     164

  Fragments arranged by size: 

                1376     164      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1292    1572 
   Size:       1292     280
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1504    1572 
   Size:       1504      68
 
XmnI GAAnn'nnTTC

Cuts at:      0    1174    1572 
   Size:       1174     398
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     ApoI     AseI    AvrII    BanII
    BbvCI    BciVI     BclI     BlpI    BmgBI     BmrI     BpmI   Bpu10I
    BsaBI    BsaWI    BseYI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI   BstAPI    BstXI     BtsI     DraI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I  HindIII     MfeI
     MmeI     MscI     MslI   MspA1I     NspI     PciI    PflMI    PpuMI
     PsiI     PstI    PvuII     SacI    SanDI     SapI     SfcI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
     BaeI    BamHI     BanI     BbeI     BbsI     BcgI    BfrBI     BglI
    BglII Bme1580I     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaHI
    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspHI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     XbaI     XhoI     XmaI     ZraI
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