Restriction map for pF1KB7813
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AgeI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BcgI        BclI        BfrI        
BglI        BglII       BlnI        BsaAI       BsaBI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DraIII      EaeI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
FbaI        FseI        FspI        HaeII       HpaI        
KasI        KpnI        KspI        MamI        MflI        
MluI        MluNI       MroI        MscI        MspA1I      
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SwaI        XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII         361  GACGTC               GACGT^C
AccI         1190  GTCGAC               GT^[AC][GT]AC
AciI         1036  CCGC                 CCGC(-3/-1)
AcsI          159  AAATTC               [GA]^AATT[CT]
AflIII         49  ACGTGT               A^C[GA][CT]GT
ApaI          385  GGGCCC               GGGCC^C
ApoI          159  AAATTC               [GA]^AATT[CT]
Asp700I       513  GAAGCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1462  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          789  GGTGCC               G^G[CT][GA]CC
BbsI         1396  GTCTTC               GAAGAC(2/6)
BbvCI        1006  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1350  GGATAC               GTATCC(6/5)
BcnI         1503  CCGGG                CC^[GC]GG
BpuAI        1396  GTCTTC               GAAGAC(2/6)
BsaI          801  GAGACC               GGTCTC(1/5)
BseRI         652  GAGGAG               GAGGAG(10/8)
BsmI         1516  GAATGC               GAATGC(1/-1)
Bsp1407I     1472  TGTACA               T^GTACA
BsrDI           4  CATTGC               GCAATG(2/0)
BsrGI        1472  TGTACA               T^GTACA
BstXI        1478  CCATTCACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         129  GCATGC               GC[ACGT]^[ACGT]GC
CfoI          639  GCGC                 GCG^C
DpnII         228  GATC                 ^GATC
DrdI         1181  GACCAAAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1494  CCATGG               C^C[GA][CT]GG
EarI          190  GAAGAG               CTCTTC(1/4)
EcoT22I         1  ATGCAT               ATGCA^T
FauI         1035  CCCGC                CCCGC(4/6)
HgaI          559  GACGC                GACGC(5/10)
HhaI          639  GCGC                 GCG^C
HinP1I        639  GCGC                 G^CGC
HincII       1190  GTCGAC               GT[CT]^[GA]AC
HindII       1190  GTCGAC               GT[CT]^[GA]AC
HindIII      1343  AAGCTT               A^AGCTT
Ksp632I       190  GAAGAG               CTCTTC(1/4)
MboI          228  GATC                 ^GATC
MfeI           95  CAATTG               C^AATTG
MunI           95  CAATTG               C^AATTG
NciI         1503  CCGGG                CC^[GC]GG
NcoI         1494  CCATGG               C^CATGG
NdeII         228  GATC                 ^GATC
NsiI            1  ATGCAT               ATGCA^T
NspI          129  GCATGC               [GA]CATG^[CT]
PflFI        1462  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         585  CCATTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI         1190  GTCGAC               G^TCGAC
Sau3AI        228  GATC                 ^GATC
SmlI          108  CTCAAG               C^T[CT][GA]AG
SphI          129  GCATGC               GCATG^C
SspBI        1472  TGTACA               T^GTACA
SspI          778  AATATT               AAT^ATT
StuI         1208  AGGCCT               AGG^CCT
Tth111I      1462  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        585  CCATTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          389  CCAATGTGCTTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          513  GAAGCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI          361  GACGTC               G[GA]^CG[CT]C
              559  GACGCC              
AhdI          803  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1264  GACCACCAGTC         
AocI          576  CCTCAGG              CC^T[ACGT]AGG
             1366  CCTAAGG             
AspEI         803  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1264  GACCACCAGTC         
BanII         385  GGGCCC               G[GA]GC[CT]^C
             1371  GGGCTC              
BlpI          943  GCTGAGC              GC^T[ACGT]AGC
             1073  GCTCAGC             
Bpu1102I      943  GCTGAGC              GC^T[ACGT]AGC
             1073  GCTCAGC             
BsaHI         361  GACGTC               G[GA]^CG[CT]C
              559  GACGCC              
BsmFI         557  GGGAC                GGGAC(10/14)
             1505  GGGAC               
BspHI         716  TCATGA               T^CATGA
             1143  TCATGA              
BspMI         804  ACCTGC               ACCTGC(4/8)
              711  GCAGGT              
BstAPI        371  GCATGGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1220  GCAATAGCTGC         
Bsu36I        576  CCTCAGG              CC^T[ACGT]AGG
             1366  CCTAAGG             
CelII         943  GCTGAGC              GC^T[ACGT]AGC
             1073  GCTCAGC             
Eam1105I      803  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1264  GACCACCAGTC         
Eco81I        576  CCTCAGG              CC^T[ACGT]AGG
             1366  CCTAAGG             
HapII         333  CCGG                 C^CGG
             1503  CCGG                
Hin1I         361  GACGTC               G[GA]^CG[CT]C
              559  GACGCC              
HpaII         333  CCGG                 C^CGG
             1503  CCGG                
MseI          925  TTAA                 T^TAA
             1294  TTAA                
MspI          333  CCGG                 C^CGG
             1503  CCGG                
NdeI          929  CATATG               CA^TATG
              961  CATATG              
PpuMI         580  AGGACCC              [GA]G^G[AT]CC[CT]
             1505  GGGACCC             
RcaI          716  TCATGA               T^CATGA
             1143  TCATGA              
SfaNI         951  GATGC                GCATC(5/9)
             1026  GATGC               
TaqI         1191  TCGA                 T^CGA
             1575  TCGA                
Tru9I         925  TTAA                 T^TAA
             1294  TTAA                
TthHB8I      1191  TCGA                 T^CGA
             1575  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AfaI        AluI        AspHI       AvaII       BbvI        
BfaI        BmyI        BpmI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
DraII       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeI        MaeII       MaeIII      MboII       MnlI        
MslI        MvaI        MwoI        NlaIII      NlaIV       
PleI        RsaI        Sau96I      ScrFI       SfcI        
StyI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       
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