Restriction map for pF1KB7771
 (Linear) (Six-base) MAPSORT of: KIBB7771.seq  Check: 4230  from: 1  to: 1350



KIBB7771.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     803     941    1350 
   Size:        803     138     409

  Fragments arranged by size: 

                 803     409     138
 
AflIII A'CryG_T

Cuts at:      0     186    1350 
   Size:        186    1164
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1071    1350 
   Size:       1071     279
 
ApoI r'AATT_y

Cuts at:      0     283     631    1350 
   Size:        283     348     719

  Fragments arranged by size: 

                 719     348     283
 
BanII G_rGCy'C

Cuts at:      0     306    1350 
   Size:        306    1044
 
Bme1580I G_kGCm'C

Cuts at:      0    1221    1350 
   Size:       1221     129
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     565    1350 
   Size:        565     785
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     712    1350 
   Size:        712     638
 
Bpu10I CC'TnA_GC

Cuts at:      0     480    1350 
   Size:        480     870
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     265    1350 
   Size:        265    1085
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1164    1194    1350 
   Size:       1164      30     156

  Fragments arranged by size: 

                1164     156      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     780     783     786     789    1304    1350 
   Size:        780       3       3       3     515      46

  Fragments arranged by size: 

                 780     515      46       3       3       3
 
BsiHKAI G_wGCw'C

Cuts at:      0    1313    1350 
   Size:       1313      37
 
Bsp1286I G_dGCh'C

Cuts at:      0     306    1221    1313    1350 
   Size:        306     915      92      37

  Fragments arranged by size: 

                 915     306      92      37
 
BspHI T'CATG_A

Cuts at:      0     954    1350 
   Size:        954     396
 
BsrGI T'GTAC_A

Cuts at:      0    1301    1350 
   Size:       1301      49
 
BssSI C'ACGA_G

Cuts at:      0     308    1021    1350 
   Size:        308     713     329

  Fragments arranged by size: 

                 713     329     308
 
BstBI TT'CG_AA

Cuts at:      0     369    1350 
   Size:        369     981
 
BstYI r'GATC_y

Cuts at:      0     618    1350 
   Size:        618     732
 
BtgI C'CryG_G

Cuts at:      0    1140    1269    1350 
   Size:       1140     129      81

  Fragments arranged by size: 

                1140     129      81
 
ClaI AT'CG_AT

Cuts at:      0     490    1350 
   Size:        490     860
 
DraI TTT'AAA

Cuts at:      0     638    1350 
   Size:        638     712
 
EarI CTCTTCn'nnn_

Cuts at:      0     733     777     807     917     937    1096    1232    1350 
   Size:        733      44      30     110      20     159     136     118

  Fragments arranged by size: 

                 733     159     136     118     110      44      30      20
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     712     803     941    1350 
   Size:        712      91     138     409

  Fragments arranged by size: 

                 712     409     138      91
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      54    1350 
   Size:         54    1296
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1057    1089    1164    1196    1350 
   Size:       1057      32      75      32     154

  Fragments arranged by size: 

                1057     154      75      32      32
 
HincII GTy'rAC

Cuts at:      0     411    1350 
   Size:        411     939
 
HindIII A'AGCT_T

Cuts at:      0     949    1350 
   Size:        949     401
 
NcoI C'CATG_G

Cuts at:      0    1269    1350 
   Size:       1269      81
 
NdeI CA'TA_TG

Cuts at:      0      99     156    1350 
   Size:         99      57    1194

  Fragments arranged by size: 

                1194      99      57
 
SapI GCTCTTCn'nnn_

Cuts at:      0     733    1232    1350 
   Size:        733     499     118

  Fragments arranged by size: 

                 733     499     118
 
StyI C'CwwG_G

Cuts at:      0     995    1269    1350 
   Size:        995     274      81

  Fragments arranged by size: 

                 995     274      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     289    1328    1350 
   Size:        289    1039      22

  Fragments arranged by size: 

                1039     289      22
 
TatI w'GTAC_w

Cuts at:      0     552    1301    1350 
   Size:        552     749      49

  Fragments arranged by size: 

                 749     552      49
 
XmnI GAAnn'nnTTC

Cuts at:      0      10    1350 
   Size:         10    1340
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     ApoI    BanII Bme1580I     BmrI     BpmI
   Bpu10I     BsaI    BsaXI    BseRI  BsiHKAI Bsp1286I    BspHI    BsrGI
    BssSI    BstBI    BstYI     BtgI     ClaI     DraI     EarI  Eco57MI
     FalI    Hin4I   HincII  HindIII     NcoI     NdeI     SapI     StyI
    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII   BstAPI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com