Restriction map for pF1KB0494
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BcgI        BclI        BfrI        
BlnI        BsaWI       BseAI       BsiWI       BspEI       
BspHI       BspLU11I    BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      DraI        
DraIII      Eam1105I    Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        MfeI        
MluI        MroI        MseI        MunI        NdeI        
NheI        NsiI        NspV        PacI        PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        SalI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspI        SwaI        Tru9I       Tth111I     XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI         1366  GTAGAC               GT^[AC][GT]AC
AcsI          896  AAATTT               [GA]^AATT[CT]
Alw44I       2044  GTGCAC               G^TGCAC
ApaLI        2044  GTGCAC               G^TGCAC
ApoI          896  AAATTT               [GA]^AATT[CT]
BamHI        2712  GGATCC               G^GATCC
BbrPI         736  CACGTG               CAC^GTG
BciVI        1384  GGATAC               GTATCC(6/5)
BglII        2672  AGATCT               A^GATCT
BsaBI        2187  GATAGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI         238  GAGACG               CGTCTC(1/5)
BsmI          963  GCATTC               GAATGC(1/-1)
Bsp1407I     1433  TGTACA               T^GTACA
BspDI        1372  ATCGAT               AT^CGAT
BsrGI        1433  TGTACA               T^GTACA
BssHII       2779  GCGCGC               G^CGCGC
ClaI         1372  ATCGAT               AT^CGAT
CpoI         2315  CGGTCCG              CG^G[AT]CCG
DrdI         2308  GACGTGCCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          504  CGGCCG               C^GGCCG
EclXI         504  CGGCCG               C^GGCCG
Eco52I        504  CGGCCG               C^GGCCG
KspI          211  CCGCGG               CCGC^GG
MamI         2187  GATAGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         1646  GCCGGC               GCC^GGC
NgoMIV       1646  GCCGGC               G^CCGGC
NotI          503  GCGGCCGC             GC^GGCCGC
NruI         2195  TCGCGA               TCG^CGA
NspI         2069  ACATGC               [GA]CATG^[CT]
PmaCI         736  CACGTG               CAC^GTG
PmlI          736  CACGTG               CAC^GTG
RsrII        2315  CGGTCCG              CG^G[AT]CCG
SacII         211  CCGCGG               CCGC^GG
SapI         1503  GAAGAGC              GCTCTTC(1/4)
SbfI         2766  CCTGCAGG             CCTGCA^GG
SexAI        1421  ACCAGGT              A^CC[AT]GGT
Sse8387I     2766  CCTGCAGG             CCTGCA^GG
SspBI        1433  TGTACA               T^GTACA
Tsp509I       897  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AatII         397  GACGTC               GACGT^C
              982  GACGTC              
AflIII        737  ACGTGT               A^C[GA][CT]GT
             2885  ACGTGT              
ApaI          222  GGGCCC               GGGCC^C
             2075  GGGCCC              
BbsI          462  GAAGAC               GAAGAC(2/6)
             2480  GTCTTC              
BbvCI        2487  CCTCAGC              CCTCAGC(-5/-2)
             1287  GCTGAGG             
BfaI          439  CTAG                 C^TAG
             1963  CTAG                
BglI          131  GCCCCAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2697  GCCCTCGAGGC         
BpuAI         462  GAAGAC               GAAGAC(2/6)
             2480  GTCTTC              
BsaAI         556  TACGTG               [CT]AC^GT[GA]
              736  CACGTG              
BsrDI        1212  CATTGC               GCAATG(2/0)
             2084  GCAATG              
EcoNI         675  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2049  CCTGCCCCAGG         
HincII        985  GTCAAC               GT[CT]^[GA]AC
             2587  GTCAAC              
HindII        985  GTCAAC               GT[CT]^[GA]AC
             2587  GTCAAC              
MaeI          439  CTAG                 C^TAG
             1963  CTAG                
PaeR7I        705  CTCGAG               C^TCGAG
             2700  CTCGAG              
SacI         1439  GAGCTC               GAGCT^C
             2446  GAGCTC              
ScaI          518  AGTACT               AGT^ACT
              779  AGTACT              
StuI         1457  AGGCCT               AGG^CCT
             2842  AGGCCT              
XhoI          705  CTCGAG               C^TCGAG
             2700  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbeI        BbvI        
BcnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        XhoII       
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