Restriction map for
pF1KB6572
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbvCI BcgI BciVI BfaI BfrI
BglII BlnI BsaBI BsaHI BsaI
BsgI BsiWI BsmI BspDI BspLU11I
BsrFI BssSI BstAPI BstEII BstPI
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DsaI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 259 GTATAC GT^[AC][GT]AC
AccIII 1169 TCCGGA T^CCGGA
AflIII 1076 ACGTGT A^C[GA][CT]GT
AlwNI 768 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1173 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 432 GAGCTC G[AT]GC[AT]^C
AvaI 950 CTCGGG C^[CT]CG[GA]G
BamHI 665 GGATCC G^GATCC
BanI 64 GGTGCC G^G[CT][GA]CC
BbrPI 1075 CACGTG CAC^GTG
BbvI 1026 GCTGC GCAGC(8/12)
BclI 458 TGATCA T^GATCA
BcnI 738 CCCGG CC^[GC]GG
BglI 67 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 879 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 879 GCTCAGC GC^T[ACGT]AGC
BsaWI 1169 TCCGGA [AT]^CCGG[AT]
BseAI 1169 TCCGGA T^CCGGA
BsiEI 464 CGGCCG CG[GA][CT]^CG
BsiHKAI 432 GAGCTC G[AT]GC[AT]^C
BsmAI 654 GAGAC GTCTC(1/5)
BsmBI 654 GAGACG CGTCTC(1/5)
BsoBI 950 CTCGGG C^[CT]CG[GA]G
Bsp1407I 340 TGTACA T^GTACA
BspEI 1169 TCCGGA T^CCGGA
BspMI 593 ACCTGC ACCTGC(4/8)
BsrBI 169 GAGCGG CCGCTC(-3/-3)
BsrGI 340 TGTACA T^GTACA
BsrI 477 ACTGG ACTGG(1/-1)
BssHII 110 GCGCGC G^CGCGC
Bst1107I 259 GTATAC GTA^TAC
BstBI 893 TTCGAA TT^CGAA
BstXI 15 CCAGCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 259 GTATAC GTA^TAC
CelII 879 GCTCAGC GC^T[ACGT]AGC
EagI 464 CGGCCG C^GGCCG
EclXI 464 CGGCCG C^GGCCG
Eco52I 464 CGGCCG C^GGCCG
EcoRI 849 GAATTC G^AATTC
FbaI 458 TGATCA T^GATCA
MroI 1169 TCCGGA T^CCGGA
MspA1I 16 CAGCTG C[AC]G^C[GT]G
NciI 738 CCCGG CC^[GC]GG
NspV 893 TTCGAA TT^CGAA
PmaCI 1075 CACGTG CAC^GTG
PmlI 1075 CACGTG CAC^GTG
PpuMI 527 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 16 CAGCTG CAG^CTG
SacI 432 GAGCTC GAGCT^C
SfuI 893 TTCGAA TT^CGAA
SmlI 1183 CTCAAG C^T[CT][GA]AG
SspBI 340 TGTACA T^GTACA
StuI 703 AGGCCT AGG^CCT
TfiI 629 GAATC G^A[AT]TC
TseI 1026 GCTGC G^C[AT]GC
XmnI 1173 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 111 CGCG CG^CG
445 CGCG
AcsI 288 GAATTT [GA]^AATT[CT]
849 GAATTC
ApaI 173 GGGCCC GGGCC^C
937 GGGCCC
ApoI 288 GAATTT [GA]^AATT[CT]
849 GAATTC
AvaII 528 GGACC G^G[AT]CC
796 GGACC
BbsI 840 GAAGAC GAAGAC(2/6)
130 GTCTTC
BpmI 265 CTGGAG CTGGAG(16/14)
748 CTGGAG
BpuAI 840 GAAGAC GAAGAC(2/6)
130 GTCTTC
BsaAI 1075 CACGTG [CT]AC^GT[GA]
1117 TACGTG
BspHI 332 TCATGA T^CATGA
455 TCATGA
BsrDI 137 GCAATG GCAATG(2/0)
905 GCAATG
BstUI 111 CGCG CG^CG
445 CGCG
BstYI 665 GGATCC [GA]^GATC[CT]
1187 AGATCC
DrdI 529 GACCCCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
677 GACAGAACAGTC
EarI 500 CTCTTC CTCTTC(1/4)
573 CTCTTC
EcoT14I 239 CCTTGG C^C[AT][AT]GG
788 CCTTGG
FauI 77 CCCGC CCCGC(4/6)
171 GCGGG
Fnu4HI 296 GCCGC GC^[ACGT]GC
1026 GCTGC
HaeII 870 AGCGCC [GA]GCGC^[CT]
1126 AGCGCC
HapII 739 CCGG C^CGG
1170 CCGG
HpaII 739 CCGG C^CGG
1170 CCGG
ItaI 296 GCCGC GC^[ACGT]GC
1026 GCTGC
Ksp632I 500 CTCTTC CTCTTC(1/4)
573 CTCTTC
MaeII 1076 ACGT A^CGT
1118 ACGT
MflI 665 GGATCC [GA]^GATC[CT]
1187 AGATCC
MseI 356 TTAA T^TAA
515 TTAA
MspI 739 CCGG C^CGG
1170 CCGG
MvnI 111 CGCG CG^CG
445 CGCG
NspI 84 ACATGC [GA]CATG^[CT]
1069 ACATGC
PflMI 984 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1100 CCACGACATGG
PstI 178 CTGCAG CTGCA^G
389 CTGCAG
RcaI 332 TCATGA T^CATGA
455 TCATGA
StyI 239 CCTTGG C^C[AT][AT]GG
788 CCTTGG
Tru9I 356 TTAA T^TAA
515 TTAA
Tsp509I 289 AATT ^AATT
850 AATT
Van91I 984 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1100 CCACGACATGG
XcmI 626 CCAGAATCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1100 CCACGACATGGCTGG
XhoII 665 GGATCC [GA]^GATC[CT]
1187 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI BalI
BanII BmyI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FokI HaeIII HhaI HinP1I HinfI
HphI MaeIII MboI MboII MluNI
MnlI MscI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI Tsp45I TspRI TthHB8I