Restriction map for pF1KB5856
 (Circular) (Six-base) MAPSORT of: pF1KB5856.seq  Check: 188  from: 1  to: 4580



pF1KB5856  4580 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AccI GT'mk_AC

Cuts at:   1618    1618 
   Size:       4580
 
Acc65I G'GTAC_C

Cuts at:   1596    1596 
   Size:       4580
 
AclI AA'CG_TT

Cuts at:   4420    4420 
   Size:       4580
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    536    1202    2405    2837    3584    3852     536 
   Size:        666    1203     432     747     268    1264

  Fragments arranged by size: 

                1264    1203     747     666     432     268
 
AflIII A'CryG_T

Cuts at:    771    1488    1932    3036    4013     771 
   Size:        717     444    1104     977    1338

  Fragments arranged by size: 

                1338    1104     977     717     444
 
AgeI A'CCGG_T

Cuts at:   2968    2968 
   Size:       4580
 
AlwNI CAG_nnn'CTG

Cuts at:   1885    3452    1885 
   Size:       1567    3013
 
ApaLI G'TGCA_C

Cuts at:   3350    3350 
   Size:       4580
 
ApoI r'AATT_y

Cuts at:    315     393     460     735    1393    1567    1584    3933    4189 
   Size:         78      67     275     658     174      17    2349     256

Cuts at:   4189     315 
   Size:        706

  Fragments arranged by size: 

                2349     706     658     275     256     174      78      67
                  17
 
AseI AT'TA_AT

Cuts at:     20     926      20 
   Size:        906    3674
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4580
 
AvaI C'yCGr_G

Cuts at:   1206    1600    1206 
   Size:        394    4186
 
BamHI G'GATC_C

Cuts at:   1605    1605 
   Size:       4580
 
BanI G'GyrC_C

Cuts at:    166    1596    2010    2258    2293     166 
   Size:       1430     414     248      35    2453

  Fragments arranged by size: 

                2453    1430     414     248      35
 
BanII G_rGCy'C

Cuts at:   1442    1594    2624    1442 
   Size:        152    1030    3398

  Fragments arranged by size: 

                3398    1030     152
 
BbeI G_GCGC'C

Cuts at:   2014    2262    2014 
   Size:        248    4332
 
BbsI GAAGACnn'nnnn_

Cuts at:   1124    1124 
   Size:       4580
 
BciVI GTATCCnnnnn_n'

Cuts at:    735    2478    3239    4580     735 
   Size:       1743     761    1341     735

  Fragments arranged by size: 

                1743    1341     761     735
 
BclI T'GATC_A

Cuts at:    372    1323    2100     372 
   Size:        951     777    2852

  Fragments arranged by size: 

                2852     951     777
 
BfrBI ATG'CAT

Cuts at:   1110    1110 
   Size:       4580
 
BglII A'GATC_T

Cuts at:   2095    4132    2095 
   Size:       2037    2543
 
BlpI GC'TnA_GC

Cuts at:    889    1692     889 
   Size:        803    3777
 
Bme1580I G_kGCm'C

Cuts at:    169    2205    2298    3354     169 
   Size:       2036      93    1056    1395

  Fragments arranged by size: 

                2036    1395    1056      93
 
BmgBI CAC'GTC

Cuts at:   1034    1200    1034 
   Size:        166    4414
 
BmrI ACTGGGnnnn_n'

Cuts at:   1792    1873    2208    4352    1792 
   Size:         81     335    2144    2020

  Fragments arranged by size: 

                2144    2020     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2947    2947 
   Size:       4580
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1073    1287    1314    1073 
   Size:        214      27    4339

  Fragments arranged by size: 

                4339     214      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1756    2635    3127    3425    1756 
   Size:        879     492     298    2911

  Fragments arranged by size: 

                2911     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4284    4284 
   Size:       4580
 
BsaAI yAC'GTr

Cuts at:   1933    1933 
   Size:       4580
 
BsaBI GATnn'nnATC

Cuts at:      6     590    2094    4131       6 
   Size:        584    1504    2037     455

  Fragments arranged by size: 

                2037    1504     584     455
 
BsaHI Gr'CG_yC

Cuts at:   2011    2259    2961    4457    2011 
   Size:        248     702    1496    2134

  Fragments arranged by size: 

                2134    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1769    2290    2968    3242    3389    3800    1769 
   Size:        521     678     274     147     411    2549

  Fragments arranged by size: 

                2549     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    106     532     106 
   Size:        426    4154
 
BseYI C'CCAG_C

Cuts at:   2005    2763    3340    3836    2005 
   Size:        758     577     496    2749

  Fragments arranged by size: 

                2749     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    2122    2168    3376     104 
   Size:       2018      46    1208    1308

  Fragments arranged by size: 

                2018    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1442    1594    2372    2562    3354    1442 
   Size:        152     778     190     792    2668

  Fragments arranged by size: 

                2668     792     778     190     152
 
BsmBI CGTCTCn'nnnn_

Cuts at:    563     563 
   Size:       4580
 
Bsp1286I G_dGCh'C

Cuts at:    169    1442    1594    2205    2298    2372    2562    2624    3354 
   Size:       1273     152     611      93      74     190      62     730

Cuts at:   3354     169 
   Size:       1395

  Fragments arranged by size: 

                1395    1273     730     611     190     152      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3800    3800 
   Size:       4580
 
BspHI T'CATG_A

Cuts at:    530    3756     530 
   Size:       3226    1354
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1630    1844    2146    2527    1630 
   Size:        214     302     381    3683

  Fragments arranged by size: 

                3683     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2872    2926    4410    4575      50 
   Size:       2822      54    1484     165      55

  Fragments arranged by size: 

                2822    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2492    2492 
   Size:       4580
 
BsrFI r'CCGG_y

Cuts at:   2578    2759    2968    2578 
   Size:        181     209    4190

  Fragments arranged by size: 

                4190     209     181
 
BsrGI T'GTAC_A

Cuts at:    620     620 
   Size:       4580
 
BssSI C'ACGA_G

Cuts at:   2851    3209    2851 
   Size:        358    4222
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1957    1957 
   Size:       4580
 
BstBI TT'CG_AA

Cuts at:   2941    2941 
   Size:       4580
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1362    1362 
   Size:       4580
 
BstYI r'GATC_y

Cuts at:      1     174     653    1500    1605    2072    2095    2430    2676 
   Size:        173     479     847     105     467      23     335     246

Cuts at:   2676    3688    3774    3786    4132       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     847     479     467     449     346     335     246
                 173     105      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     170     107 
   Size:         63    4517
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1266    2530    2711    2874    1266 
   Size:       1264     181     163    2972

  Fragments arranged by size: 

                2972    1264     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1823    1823 
   Size:       4580
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1452    1487    1452 
   Size:         35    4545
 
DraI TTT'AAA

Cuts at:   1289    1579    1289 
   Size:        290    4290
 
DrdI GACnn_nn'nnGTC

Cuts at:   2286    3144    2286 
   Size:        858    3722
 
EaeI y'GGCC_r

Cuts at:   2165    2339    2730    2757    4102    2165 
   Size:        174     391      27    1345    2643

  Fragments arranged by size: 

                2643    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2165    2165 
   Size:       4580
 
EarI CTCTTCn'nnn_

Cuts at:   1070    2603    2813    1070 
   Size:       1533     210    2837

  Fragments arranged by size: 

                2837    1533     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2788    3098    3244    4390    2788 
   Size:        310     146    1146    2978

  Fragments arranged by size: 

                2978    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1440    1592    1440 
   Size:        152    4428
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    536    1073    1202    1287    1314    2405    2837    3584    3852 
   Size:        537     129      85      27    1091     432     747     268

Cuts at:   3852     536 
   Size:       1264

  Fragments arranged by size: 

                1264    1091     747     537     432     268     129      85
                  27
 
EcoO109I rG'GnC_Cy

Cuts at:    273    1719     273 
   Size:       1446    3134
 
EcoRI G'AATT_C

Cuts at:    315     735    1584     315 
   Size:        420     849    3311

  Fragments arranged by size: 

                3311     849     420
 
EcoRV GAT'ATC

Cuts at:    507     507 
   Size:       4580
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    862    1007    2599     862 
   Size:        145    1592    2843

  Fragments arranged by size: 

                2843    1592     145
 
FspI TGC'GCA

Cuts at:   2361    2361 
   Size:       4580
 
HaeII r_GCGC'y

Cuts at:   2014    2262    3284    4263    2014 
   Size:        248    1022     979    2331

  Fragments arranged by size: 

                2331    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1069    1101    1424    1456    2422    2452    2454    2484    1069 
   Size:         32     323      32     966      30       2      30    3165

  Fragments arranged by size: 

                3165     966     323      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1619    1619 
   Size:       4580
 
KasI G'GCGC_C

Cuts at:   2010    2258    2010 
   Size:        248    4332
 
KpnI G_GTAC'C

Cuts at:   1600    1600 
   Size:       4580
 
MfeI C'AATT_G

Cuts at:    215     215 
   Size:       4580
 
MluI A'CGCG_T

Cuts at:   4013    4013 
   Size:       4580
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    405    3251    3435     405 
   Size:       2846     184    1550

  Fragments arranged by size: 

                2846    1550     184
 
MscI TGG'CCA

Cuts at:   2341    2341 
   Size:       4580
 
MslI CAynn'nnrTG

Cuts at:    380     616    2569    2696     380 
   Size:        236    1953     127    2264

  Fragments arranged by size: 

                2264    1953     236     127
 
MspA1I CmG'CkG

Cuts at:    172    1358    1691    2005    2365    3378    3623     172 
   Size:       1186     333     314     360    1013     245    1129

  Fragments arranged by size: 

                1186    1129    1013     360     333     314     245
 
NaeI GCC'GGC

Cuts at:   2761    2761 
   Size:       4580
 
NarI GG'CG_CC

Cuts at:   2011    2259    2011 
   Size:        248    4332
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4580
 
NgoMIV G'CCGG_C

Cuts at:   2759    2759 
   Size:       4580
 
NsiI A_TGCA'T

Cuts at:   1112    1112 
   Size:       4580
 
NspI r_CATG'y

Cuts at:    775    1110    1492    1633    2133    3040     775 
   Size:        335     382     141     500     907    2315

  Fragments arranged by size: 

                2315     907     500     382     335     141
 
PciI A'CATG_T

Cuts at:    771    1488    3036     771 
   Size:        717    1548    2315

  Fragments arranged by size: 

                2315    1548     717
 
PfoI T'CCnGG_A

Cuts at:   1125    1125 
   Size:       4580
 
PmeI GTTT'AAAC

Cuts at:   1579    1579 
   Size:       4580
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1224    1256    3719    3751    1224 
   Size:         32    2463      32    2053

  Fragments arranged by size: 

                2463    2053      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    273     273 
   Size:       4580
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    825     857     825 
   Size:         32    4548
 
PstI C_TGCA'G

Cuts at:   1627    2312    1627 
   Size:        685    3895
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4580
 
PvuII CAG'CTG

Cuts at:   1358    2005    2365    1358 
   Size:        647     360    3573

  Fragments arranged by size: 

                3573     647     360
 
RsrII CG'GwC_CG

Cuts at:   2775    2775 
   Size:       4580
 
SacI G_AGCT'C

Cuts at:   1442    1594    1442 
   Size:        152    4428
 
SacII CC_GC'GG

Cuts at:    173     173 
   Size:       4580
 
SalI G'TCGA_C

Cuts at:   1617    1617 
   Size:       4580
 
SapI GCTCTTCn'nnn_

Cuts at:   1070    2603    2813    1070 
   Size:       1533     210    2837

  Fragments arranged by size: 

                2837    1533     210
 
SbfI CC_TGCA'GG

Cuts at:   1627    1627 
   Size:       4580
 
ScaI AGT'ACT

Cuts at:    745    4097     745 
   Size:       3352    1228
 
SfcI C'TryA_G

Cuts at:     33    1623    2308    3301    3492      33 
   Size:       1590     685     993     191    1121

  Fragments arranged by size: 

                1590    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   2012    2260    2012 
   Size:        248    4332
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4580
 
SmaI CCC'GGG

Cuts at:   1208    1602    1208 
   Size:        394    4186
 
SmlI C'TyrA_G

Cuts at:   1735    2650    3142    3404    1735 
   Size:        915     492     262    2911

  Fragments arranged by size: 

                2911     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4580
 
SphI G_CATG'C

Cuts at:   1110    1633    2133    1110 
   Size:        523     500    3557

  Fragments arranged by size: 

                3557     523     500
 
SspI AAT'ATT

Cuts at:    345     366     953    1191     345 
   Size:         21     587     238    3734

  Fragments arranged by size: 

                3734     587     238      21
 
StyI C'CwwG_G

Cuts at:    107    1714    2068     107 
   Size:       1607     354    2619

  Fragments arranged by size: 

                2619    1607     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2163    2296    2755    2964    2163 
   Size:        133     459     209    3779

  Fragments arranged by size: 

                3779     459     209     133
 
TatI w'GTAC_w

Cuts at:    620     743    4095     620 
   Size:        123    3352    1105

  Fragments arranged by size: 

                3352    1105     123
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4097    4129    4097 
   Size:         32    4548
 
Tth111I GACn'n_nGTC

Cuts at:   1201    2377    1201 
   Size:       1176    3404
 
XbaI T'CTAG_A

Cuts at:   1611    1611 
   Size:       4580
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1270    2075    1270 
   Size:        805    3775
 
XmaI C'CCGG_G

Cuts at:   1206    1600    1206 
   Size:        394    4186
 
XmnI GAAnn'nnTTC

Cuts at:   1190    1311    1190 
   Size:        121    4459
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NsiI     NspI     PciI     PfoI     PmeI
     PpiI    PpuMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI     BglI     BmtI
   Bpu10I    BsaXI     BsgI    BsiWI     BsmI   BssHII   BstEII  BstZ17I
   Bsu36I     ClaI   DraIII    EcoNI     FseI    FspAI  HindIII     HpaI
     NdeI     NheI     NotI     NruI     PacI     PasI    PflMI     PmlI
    PshAI     PsiI   PspOMI    PspXI    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     StuI     SwaI     XhoI     ZraI
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