Restriction map for pF1KE0988
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Aor51HI     ApoI        
AscI        AseI        AsnI        AspEI       AspI        
AvrII       BamHI       BbeI        BbrPI       BcgI        
BclI        BfaI        BfrI        BglII       BlnI        
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        DrdI        Eam1105I    Eco47III    
EcoRI       EcoRV       FbaI        FseI        HindIII     
KasI        MaeI        MamI        MfeI        MroI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SalI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tsp509I     
Tth111I     Van91I      XbaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I        488  GGTACC               G^GTACC
AflIII        505  ACGCGT               A^C[GA][CT]GT
AocI          785  CCTCAGG              CC^T[ACGT]AGG
ApaI         1816  GGGCCC               GGGCC^C
Asp700I       984  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       488  GGTACC               G^GTACC
AvaII         235  GGACC                G^G[AT]CC
BciVI         871  GGATAC               GTATCC(6/5)
BcnI          334  CCGGG                CC^[GC]GG
BglI          784  GCCTCAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1487  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1487  GCTCAGC              GC^T[ACGT]AGC
BsaAI         376  TACGTG               [CT]AC^GT[GA]
BspHI        1712  TCATGA               T^CATGA
BstEII       1578  GGTCACC              G^GT[ACGT]ACC
BstPI        1578  GGTCACC              G^GT[ACGT]ACC
Bsu36I        785  CCTCAGG              CC^T[ACGT]AGG
CelII        1487  GCTCAGC              GC^T[ACGT]AGC
Eco81I        785  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      1578  GGTCACC              G^GT[ACGT]ACC
EcoT22I      1527  ATGCAT               ATGCA^T
HaeII         185  AGCGCC               [GA]GCGC^[CT]
HpaI         1408  GTTAAC               GTT^AAC
HphI         1580  TCACC                GGTGA(8/7)
KpnI          488  GGTACC               GGTAC^C
MluI          505  ACGCGT               A^CGCGT
MseI         1409  TTAA                 T^TAA
NaeI          214  GCCGGC               GCC^GGC
NciI          334  CCGGG                CC^[GC]GG
NgoMIV        214  GCCGGC               G^CCGGC
NsiI         1527  ATGCAT               ATGCA^T
PaeR7I        322  CTCGAG               C^TCGAG
PleI          831  GACTC                GAGTC(4/5)
RcaI         1712  TCATGA               T^CATGA
ScaI          659  AGTACT               AGT^ACT
SexAI        1328  ACCAGGT              A^CC[AT]GGT
TfiI          848  GAATC                G^A[AT]TC
Tru9I        1409  TTAA                 T^TAA
XhoI          322  CTCGAG               C^TCGAG
XmnI          984  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   41 enzymes) #
###################################

AcyI          358  GACGCC               G[GA]^CG[CT]C
             1207  GGCGTC              
Alw44I        151  GTGCAC               G^TGCAC
             1372  GTGCAC              
AlwNI         112  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              788  CAGGGGCTG           
ApaLI         151  GTGCAC               G^TGCAC
             1372  GTGCAC              
AvaI          322  CTCGAG               C^[CT]CG[GA]G
              670  CCCGAG              
AviII         293  TGCGCA               TGC^GCA
             1076  TGCGCA              
BalI         1097  TGGCCA               TGG^CCA
             1799  TGGCCA              
BbsI          615  GAAGAC               GAAGAC(2/6)
              340  GTCTTC              
BbvCI         564  GCTGAGG              CCTCAGC(-5/-2)
             1840  GCTGAGG             
BpuAI         615  GAAGAC               GAAGAC(2/6)
              340  GTCTTC              
BsaHI         358  GACGCC               G[GA]^CG[CT]C
             1207  GGCGTC              
BsgI         1264  CTGCAC               GTGCAG(16/14)
             1324  CTGCAC              
BsiEI         310  CGGCCG               CG[GA][CT]^CG
              916  CGGCCG              
BsmAI         356  GAGAC                GTCTC(1/5)
              843  GAGAC               
BsmBI         356  GAGACG               CGTCTC(1/5)
              843  GAGACG              
BsmFI         600  GTCCC                GGGAC(10/14)
              900  GTCCC               
BsmI         1525  GAATGC               GAATGC(1/-1)
              665  GCATTC              
BsoBI         322  CTCGAG               C^[CT]CG[GA]G
              670  CCCGAG              
BsrI         1455  ACTGG                ACTGG(1/-1)
              111  CCAGT               
BstXI        1448  CCAAAGCACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1802  CCAGCAAGGTGG        
BstYI        1295  AGATCC               [GA]^GATC[CT]
             1727  GGATCT              
EagI          310  CGGCCG               C^GGCCG
              916  CGGCCG              
EclXI         310  CGGCCG               C^GGCCG
              916  CGGCCG              
Eco52I        310  CGGCCG               C^GGCCG
              916  CGGCCG              
EcoNI         411  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              747  CCTGAAGGAGG         
FspI          293  TGCGCA               TGC^GCA
             1076  TGCGCA              
Hin1I         358  GACGCC               G[GA]^CG[CT]C
             1207  GGCGTC              
HincII        828  GTTGAC               GT[CT]^[GA]AC
             1408  GTTAAC              
HindII        828  GTTGAC               GT[CT]^[GA]AC
             1408  GTTAAC              
HinfI         831  GACTC                G^A[ACGT]TC
              848  GAATC               
MflI         1295  AGATCC               [GA]^GATC[CT]
             1727  GGATCT              
MluNI        1097  TGGCCA               TGG^CCA
             1799  TGGCCA              
MscI         1097  TGGCCA               TGG^CCA
             1799  TGGCCA              
NspI         1073  GCATGC               [GA]CATG^[CT]
             1775  GCATGC              
PvuII         700  CAGCTG               CAG^CTG
             1174  CAGCTG              
SacI          244  GAGCTC               GAGCT^C
              970  GAGCTC              
SapI           96  GCTCTTC              GCTCTTC(1/4)
              987  GCTCTTC             
SfcI          954  CTACAG               C^T[GA][CT]AG
             1791  CTACAG              
SphI         1073  GCATGC               GCATG^C
             1775  GCATGC              
StuI          755  AGGCCT               AGG^CCT
             1592  AGGCCT              
XhoII        1295  AGATCC               [GA]^GATC[CT]
             1727  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       BanI        BanII       BbvI        BmyI        
BpmI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HpaII       
ItaI        Ksp632I     KspI        MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       RsaI        SacII       
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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