Restriction map for pF1KE0988
 (Circular) (Six-base) MAPSORT of: pF1KE0988.seq  Check: 8500  from: 1  to: 4970



pF1KE0988  4970 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    137     540     137 
   Size:        403    4567
 
AccI GT'mk_AC

Cuts at:   2008    2008 
   Size:       4970
 
Acc65I G'GTAC_C

Cuts at:    596    1986     596 
   Size:       1390    3580
 
AclI AA'CG_TT

Cuts at:   4810    4810 
   Size:       4970
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    199     844     850     877    1123    1240    2795    3227    3974 
   Size:        645       6      27     246     117    1555     432     747

Cuts at:   3974    4242     199 
   Size:        268     927

  Fragments arranged by size: 

                1555     927     747     645     432     268     246     117
                  27       6
 
AflIII A'CryG_T

Cuts at:    613    2322    3426    4403     613 
   Size:       1709    1104     977    1180

  Fragments arranged by size: 

                1709    1180    1104     977
 
AgeI A'CCGG_T

Cuts at:   3358    3358 
   Size:       4970
 
AleI CACnn'nnGTG

Cuts at:   1045    1794    1045 
   Size:        749    4221
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    372     685    1525    1650     372 
   Size:        313     840     125    3692

  Fragments arranged by size: 

                3692     840     313     125
 
AlwNI CAG_nnn'CTG

Cuts at:    225     901    2275    3842     225 
   Size:        676    1374    1567    1353

  Fragments arranged by size: 

                1567    1374    1353     676
 
ApaI G_GGCC'C

Cuts at:   1928    1928 
   Size:       4970
 
ApaLI G'TGCA_C

Cuts at:    259    1480    3740     259 
   Size:       1221    2260    1489

  Fragments arranged by size: 

                2260    1489    1221
 
ApoI r'AATT_y

Cuts at:   1974    4323    4579    1974 
   Size:       2349     256    2365

  Fragments arranged by size: 

                2365    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4970
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4970
 
AvaI C'yCGr_G

Cuts at:    430     778    1990     430 
   Size:        348    1212    3410

  Fragments arranged by size: 

                3410    1212     348
 
BamHI G'GATC_C

Cuts at:   1995    1995 
   Size:       4970
 
BanI G'GyrC_C

Cuts at:    307     596     784    1567    1986    2400    2648    2683     307 
   Size:        289     188     783     419     414     248      35    2594

  Fragments arranged by size: 

                2594     783     419     414     289     248     188      35
            
 
BanII G_rGCy'C

Cuts at:    206     356     845    1060    1082    1928    1984    3014     206 
   Size:        150     489     215      22     846      56    1030    2162

  Fragments arranged by size: 

                2162    1030     846     489     215     150      56      22
            
 
BbeI G_GCGC'C

Cuts at:   2404    2652    2404 
   Size:        248    4722
 
BbsI GAAGACnn'nnnn_

Cuts at:    441     730     441 
   Size:        289    4681
 
BbvCI CC'TCA_GC

Cuts at:    673    1949     673 
   Size:       1276    3694
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4936
 
BciVI GTATCCnnnnn_n'

Cuts at:    973    2868    3629    4970     973 
   Size:       1895     761    1341     973

  Fragments arranged by size: 

                1895    1341     973     761
 
BclI T'GATC_A

Cuts at:   2490    2490 
   Size:       4970
 
BfrBI ATG'CAT

Cuts at:   1637    1637 
   Size:       4970
 
BglI GCCn_nnn'nGGC

Cuts at:    898     898 
   Size:       4970
 
BglII A'GATC_T

Cuts at:   2485    4522    2485 
   Size:       2037    2933
 
BlpI GC'TnA_GC

Cuts at:   1596    2082    1596 
   Size:        486    4484
 
Bme1580I G_kGCm'C

Cuts at:    263     697     787    1231    1484    1789    1928    2595    2688 
   Size:        434      90     444     253     305     139     667      93

Cuts at:   2688    3744     263 
   Size:       1056    1489

  Fragments arranged by size: 

                1489    1056     667     444     434     305     253     139
                  93      90
 
BmgBI CAC'GTC

Cuts at:    497    1203     497 
   Size:        706    4264
 
BmrI ACTGGGnnnn_n'

Cuts at:   1573    2182    2263    2598    4742    1573 
   Size:        609      81     335    2144    1801

  Fragments arranged by size: 

                2144    1801     609     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    384    3337     384 
   Size:       2953    2017
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    526     541    1483    1641    1695     526 
   Size:         15     942     158      54    3801

  Fragments arranged by size: 

                3801     942     158      54      15
 
Bpu10I CC'TnA_GC

Cuts at:    673     989    1949     673 
   Size:        316     960    3694

  Fragments arranged by size: 

                3694     960     316
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1066    1600    2146    3025    3517    3815    1066 
   Size:        534     546     879     492     298    2221

  Fragments arranged by size: 

                2221     879     546     534     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4674    4674 
   Size:       4970
 
BsaAI yAC'GTr

Cuts at:    486    2323     486 
   Size:       1837    3133
 
BsaBI GATnn'nnATC

Cuts at:      6    2484    4521       6 
   Size:       2478    2037     455

  Fragments arranged by size: 

                2478    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    467    1316    2401    2649    3351    4847     467 
   Size:        849    1085     248     702    1496     590

  Fragments arranged by size: 

                1496    1085     849     702     590     248
 
BsaWI w'CCGG_w

Cuts at:   2159    2680    3358    3632    3779    4190    2159 
   Size:        521     678     274     147     411    2939

  Fragments arranged by size: 

                2939     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1690    1720    1690 
   Size:         30    4940
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    184     634    1090    1108    1414    1480     184 
   Size:        450     456      18     306      66    3674

  Fragments arranged by size: 

                3674     456     450     306      66      18
 
BseYI C'CCAG_C

Cuts at:    234     918    1357    2395    3153    3730    4226     234 
   Size:        684     439    1038     758     577     496     978

  Fragments arranged by size: 

                1038     978     758     684     577     496     439
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1357    1417    1357 
   Size:         60    4910
 
BsiEI CG_ry'CG

Cuts at:    104     421    1027    2512    2558    3766     104 
   Size:        317     606    1485      46    1208    1308

  Fragments arranged by size: 

                1485    1308    1208     606     317      46
 
BsiHKAI G_wGCw'C

Cuts at:    176     263     356    1082    1484    1984    2762    2952    3744 
   Size:         87      93     726     402     500     778     190     792

Cuts at:   3744     176 
   Size:       1402

  Fragments arranged by size: 

                1402     792     778     726     500     402     190      93
                  87
 
BsmI GAATG_Cn'

Cuts at:    773    1639     773 
   Size:        866    4104
 
BsmBI CGTCTCn'nnnn_

Cuts at:    458     945     458 
   Size:        487    4483
 
Bsp1286I G_dGCh'C

Cuts at:    176     206     263     356     697     787     845    1060    1082 
   Size:         30      57      93     341      90      58     215      22

Cuts at:   1082    1231    1484    1789    1928    1984    2595    2688    2762 
   Size:        149     253     305     139      56     611      93      74

Cuts at:   2762    2952    3014    3744     176 
   Size:        190      62     730    1402

  Fragments arranged by size: 

                1402     730     611     341     305     253     215     190
                 149     139      93      93      90      74      62      58
                  57      56      30      22
 
BspEI T'CCGG_A

Cuts at:   4190    4190 
   Size:       4970
 
BspHI T'CATG_A

Cuts at:   1820    4146    1820 
   Size:       2326    2644
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    137     407     540    2020    2234    2536    2917     137 
   Size:        270     133    1480     214     302     381    2190

  Fragments arranged by size: 

                2190    1480     381     302     270     214     133
 
BsrBI CCG'CTC

Cuts at:     50     585    1595    1614    3262    3316    4800    4965      50 
   Size:        535    1010      19    1648      54    1484     165      55

  Fragments arranged by size: 

                1648    1484    1010     535     165      55      54      19
            
 
BsrDI GCAATG_nn'

Cuts at:    317     563    1422    2882     317 
   Size:        246     859    1460    2405

  Fragments arranged by size: 

                2405    1460     859     246
 
BsrFI r'CCGG_y

Cuts at:    322     345     416    1520    2968    3149    3358     322 
   Size:         23      71    1104    1448     181     209    1934

  Fragments arranged by size: 

                1934    1448    1104     209     181      71      23
 
BssSI C'ACGA_G

Cuts at:    256     580    1041    3241    3599     256 
   Size:        324     461    2200     358    1627

  Fragments arranged by size: 

                2200    1627     461     358     324
 
BstAPI GCAn_nnn'nTGC

Cuts at:    267     410     770    1275    1567    1884    2347     267 
   Size:        143     360     505     292     317     463    2890

  Fragments arranged by size: 

                2890     505     463     360     317     292     143
 
BstBI TT'CG_AA

Cuts at:   3331    3331 
   Size:       4970
 
BstEII G'GTnAC_C

Cuts at:   1686    1686 
   Size:       4970
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1563    1917    1563 
   Size:        354    4616
 
BstYI r'GATC_y

Cuts at:      1    1403    1835    1995    2462    2485    2820    3066    4078 
   Size:       1402     432     160     467      23     335     246    1012

Cuts at:   4078    4164    4176    4522       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1402    1012     467     449     432     346     335     246
                 160      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    894     894 
   Size:       4970
 
BtgI C'CryG_G

Cuts at:    119     470     679    1021    1034    1445    1871     119 
   Size:        351     209     342      13     411     426    3218

  Fragments arranged by size: 

                3218     426     411     351     342     209      13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1682    2920    3101    3264    1682 
   Size:       1238     181     163    3388

  Fragments arranged by size: 

                3388    1238     181     163
 
BtsI GCAGTG_nn'

Cuts at:    885    1586    2213     885 
   Size:        701     627    3642

  Fragments arranged by size: 

                3642     701     627
 
DraI TTT'AAA

Cuts at:   1969    1969 
   Size:       4970
 
DraIII CAC_nnn'GTG

Cuts at:    267    1440    1567    1694     267 
   Size:       1173     127     127    3543

  Fragments arranged by size: 

                3543    1173     127     127
 
DrdI GACnn_nn'nnGTC

Cuts at:   2676    3534    2676 
   Size:        858    4112
 
EaeI y'GGCC_r

Cuts at:    418    1024    1205    1421    1609    1713    1907    2555    2729 
   Size:        606     181     216     188     104     194     648     174

Cuts at:   2729    3120    3147    4492     418 
   Size:        391      27    1345     896

  Fragments arranged by size: 

                1345     896     648     606     391     216     194     188
                 181     174     104      27
 
EagI C'GGCC_G

Cuts at:    418    1024    2555     418 
   Size:        606    1531    2833

  Fragments arranged by size: 

                2833    1531     606
 
EarI CTCTTCn'nnn_

Cuts at:    211    1102    1244    2993    3203     211 
   Size:        891     142    1749     210    1978

  Fragments arranged by size: 

                1978    1749     891     210     142
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    211     850    3178    3488    3634    4780     211 
   Size:        639    2328     310     146    1146     401

  Fragments arranged by size: 

                2328    1146     639     401     310     146
 
EcoICRI GAG'CTC

Cuts at:    354    1080    1982     354 
   Size:        726     902    3342

  Fragments arranged by size: 

                3342     902     726
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    199     526     541     844     850     877    1123    1240    1483 
   Size:        327      15     303       6      27     246     117     243

Cuts at:   1483    1641    1695    2795    3227    3974    4242     199 
   Size:        158      54    1100     432     747     268     927

  Fragments arranged by size: 

                1100     927     747     432     327     303     268     246
                 243     158     117      54      27      15       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:    523     859     523 
   Size:        336    4634
 
EcoO109I rG'GnC_Cy

Cuts at:    516     543    1332    1924    1925    2109     516 
   Size:         27     789     592       1     184    3377

  Fragments arranged by size: 

                3377     789     592     184      27       1
 
EcoRI G'AATT_C

Cuts at:   1974    1974 
   Size:       4970
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1113    2989    1113 
   Size:       1876    3094
 
FspI TGC'GCA

Cuts at:    403    1186    2751     403 
   Size:        783    1565    2622

  Fragments arranged by size: 

                2622    1565     783
 
HaeII r_GCGC'y

Cuts at:    297    2404    2652    3674    4653     297 
   Size:       2107     248    1022     979     614

  Fragments arranged by size: 

                2107    1022     979     614     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    930     962    1690    1722    2812    2842    2844    2874     930 
   Size:         32     728      32    1090      30       2      30    3026

  Fragments arranged by size: 

                3026    1090     728      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    938    1518    2009     938 
   Size:        580     491    3899

  Fragments arranged by size: 

                3899     580     491
 
HpaI GTT'AAC

Cuts at:   1518    1518 
   Size:       4970
 
KasI G'GCGC_C

Cuts at:   2400    2648    2400 
   Size:        248    4722
 
KpnI G_GTAC'C

Cuts at:    600    1990     600 
   Size:       1390    3580
 
MluI A'CGCG_T

Cuts at:    613    4403     613 
   Size:       3790    1180
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3641    3825    3641 
   Size:        184    4786
 
MscI TGG'CCA

Cuts at:   1207    1909    2731    1207 
   Size:        702     822    3446

  Fragments arranged by size: 

                3446     822     702
 
MslI CAynn'nnrTG

Cuts at:   1045    1213    1351    1450    1632    1794    2959    3086    1045 
   Size:        168     138      99     182     162    1165     127    2929

  Fragments arranged by size: 

                2929    1165     182     168     162     138     127      99
            
 
MspA1I CmG'CkG

Cuts at:    121     681     810    1023    1284    1371    2081    2395    2755 
   Size:        560     129     213     261      87     710     314     360

Cuts at:   2755    3768    4013     121 
   Size:       1013     245    1078

  Fragments arranged by size: 

                1078    1013     710     560     360     314     261     245
                 213     129      87
 
NaeI GCC'GGC

Cuts at:    324    3151     324 
   Size:       2827    2143
 
NarI GG'CG_CC

Cuts at:   2401    2649    2401 
   Size:        248    4722
 
NcoI C'CATG_G

Cuts at:    470    1034    1445     470 
   Size:        564     411    3995

  Fragments arranged by size: 

                3995     564     411
 
NgoMIV G'CCGG_C

Cuts at:    322    3149     322 
   Size:       2827    2143
 
NsiI A_TGCA'T

Cuts at:   1639    1639 
   Size:       4970
 
NspI r_CATG'y

Cuts at:   1185    1887    2023    2523    3430    1185 
   Size:        702     136     500     907    2725

  Fragments arranged by size: 

                2725     907     702     500     136
 
PciI A'CATG_T

Cuts at:   3426    3426 
   Size:       4970
 
PmeI GTTT'AAAC

Cuts at:   1969    1969 
   Size:       4970
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4109    4141    4109 
   Size:         32    4938
 
PspOMI G'GGCC_C

Cuts at:   1924    1924 
   Size:       4970
 
PspXI vC'TCGA_Gb

Cuts at:    430     430 
   Size:       4970
 
PstI C_TGCA'G

Cuts at:   2017    2702    2017 
   Size:        685    4285
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4970
 
PvuII CAG'CTG

Cuts at:    810    1284    2395    2755     810 
   Size:        474    1111     360    3025

  Fragments arranged by size: 

                3025    1111     474     360
 
RsrII CG'GwC_CG

Cuts at:   3165    3165 
   Size:       4970
 
SacI G_AGCT'C

Cuts at:    356    1082    1984     356 
   Size:        726     902    3342

  Fragments arranged by size: 

                3342     902     726
 
SacII CC_GC'GG

Cuts at:    122     682    1024     122 
   Size:        560     342    4068

  Fragments arranged by size: 

                4068     560     342
 
SalI G'TCGA_C

Cuts at:   2007    2007 
   Size:       4970
 
SapI GCTCTTCn'nnn_

Cuts at:    211    1102    2993    3203     211 
   Size:        891    1891     210    1978

  Fragments arranged by size: 

                1978    1891     891     210
 
SbfI CC_TGCA'GG

Cuts at:   2017    2017 
   Size:       4970
 
ScaI AGT'ACT

Cuts at:    769    4487     769 
   Size:       3718    1252
 
SexAI A'CCwGG_T

Cuts at:   1436    1436 
   Size:       4970
 
SfcI C'TryA_G

Cuts at:     33    1062    1899    2013    2698    3691    3882      33 
   Size:       1029     837     114     685     993     191    1121

  Fragments arranged by size: 

                1121    1029     993     837     685     191     114
 
SfoI GGC'GCC

Cuts at:   2402    2650    2402 
   Size:        248    4722
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4970
 
SmaI CCC'GGG

Cuts at:   1992    1992 
   Size:       4970
 
SmlI C'TyrA_G

Cuts at:    430    1081    1615    2125    3040    3532    3794     430 
   Size:        651     534     510     915     492     262    1606

  Fragments arranged by size: 

                1606     915     651     534     510     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4970
 
SphI G_CATG'C

Cuts at:   1185    1887    2023    2523    1185 
   Size:        702     136     500    3632

  Fragments arranged by size: 

                3632     702     500     136
 
StuI AGG'CCT

Cuts at:    865    1702     865 
   Size:        837    4133
 
StyI C'CwwG_G

Cuts at:    470    1034    1445    2104    2458     470 
   Size:        564     411     659     354    2982

  Fragments arranged by size: 

                2982     659     564     411     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    670     941    1555    2553    2686    3145    3354     670 
   Size:        271     614     998     133     459     209    2286

  Fragments arranged by size: 

                2286     998     614     459     271     209     133
 
TatI w'GTAC_w

Cuts at:    767    4485     767 
   Size:       3718    1252
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1426    1458    4487    4519    1426 
   Size:         32    3029      32    1877

  Fragments arranged by size: 

                3029    1877      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2767    2767 
   Size:       4970
 
XbaI T'CTAG_A

Cuts at:   2001    2001 
   Size:       4970
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2465    2465 
   Size:       4970
 
XhoI C'TCGA_G

Cuts at:    430     430 
   Size:       4970
 
XmaI C'CCGG_G

Cuts at:   1990    1990 
   Size:       4970
 
XmnI GAAnn'nnTTC

Cuts at:   1096    1096 
   Size:       4970
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI
     NspI     PciI     PmeI     PpiI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AloI     AscI    AvrII     BaeI
     BmtI    BsiWI    BsrGI   BssHII  BstZ17I     ClaI    CspCI    EcoRV
     FseI    FspAI  HindIII     MfeI     NdeI     NheI     NotI     NruI
     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI
     PsrI    SanDI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
     ZraI
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