Restriction map for pF1KE0988
 (Linear) (Six-base) MAPSORT of: KIEE0988.seq  Check: 9347  from: 1  to: 1857



KIEE0988.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      29     432    1857 
   Size:         29     403    1425

  Fragments arranged by size: 

                1425     403      29
 
Acc65I G'GTAC_C

Cuts at:      0     488    1857 
   Size:        488    1369
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     736     742     769    1015    1132    1857 
   Size:         91     645       6      27     246     117     725

  Fragments arranged by size: 

                 725     645     246     117      91      27       6
 
AflIII A'CryG_T

Cuts at:      0     505    1857 
   Size:        505    1352
 
AleI CACnn'nnGTG

Cuts at:      0     937    1686    1857 
   Size:        937     749     171

  Fragments arranged by size: 

                 937     749     171
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     264     577    1417    1542    1857 
   Size:        264     313     840     125     315

  Fragments arranged by size: 

                 840     315     313     264     125
 
AlwNI CAG_nnn'CTG

Cuts at:      0     117     793    1857 
   Size:        117     676    1064

  Fragments arranged by size: 

                1064     676     117
 
ApaI G_GGCC'C

Cuts at:      0    1820    1857 
   Size:       1820      37
 
ApaLI G'TGCA_C

Cuts at:      0     151    1372    1857 
   Size:        151    1221     485

  Fragments arranged by size: 

                1221     485     151
 
AvaI C'yCGr_G

Cuts at:      0     322     670    1857 
   Size:        322     348    1187

  Fragments arranged by size: 

                1187     348     322
 
BanI G'GyrC_C

Cuts at:      0     199     488     676    1459    1857 
   Size:        199     289     188     783     398

  Fragments arranged by size: 

                 783     398     289     199     188
 
BanII G_rGCy'C

Cuts at:      0      98     248     737     952     974    1820    1857 
   Size:         98     150     489     215      22     846      37

  Fragments arranged by size: 

                 846     489     215     150      98      37      22
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     333     622    1857 
   Size:        333     289    1235

  Fragments arranged by size: 

                1235     333     289
 
BbvCI CC'TCA_GC

Cuts at:      0     565    1841    1857 
   Size:        565    1276      16

  Fragments arranged by size: 

                1276     565      16
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     865    1857 
   Size:        865     992
 
BfrBI ATG'CAT

Cuts at:      0    1529    1857 
   Size:       1529     328
 
BglI GCCn_nnn'nGGC

Cuts at:      0     790    1857 
   Size:        790    1067
 
BlpI GC'TnA_GC

Cuts at:      0    1488    1857 
   Size:       1488     369
 
Bme1580I G_kGCm'C

Cuts at:      0     155     589     679    1123    1376    1681    1820    1857 
   Size:        155     434      90     444     253     305     139      37

  Fragments arranged by size: 

                 444     434     305     253     155     139      90      37
            
 
BmgBI CAC'GTC

Cuts at:      0     389    1095    1857 
   Size:        389     706     762

  Fragments arranged by size: 

                 762     706     389
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1465    1857 
   Size:       1465     392
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     276    1857 
   Size:        276    1581
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     433    1375    1533    1587    1857 
   Size:        418      15     942     158      54     270

  Fragments arranged by size: 

                 942     418     270     158      54      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     565     881    1841    1857 
   Size:        565     316     960      16

  Fragments arranged by size: 

                 960     565     316      16
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     958    1492    1857 
   Size:        958     534     365

  Fragments arranged by size: 

                 958     534     365
 
BsaAI yAC'GTr

Cuts at:      0     378    1857 
   Size:        378    1479
 
BsaHI Gr'CG_yC

Cuts at:      0     359    1208    1857 
   Size:        359     849     649

  Fragments arranged by size: 

                 849     649     359
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1582    1612    1857 
   Size:       1582      30     245

  Fragments arranged by size: 

                1582     245      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76     526     982    1000    1306    1372    1857 
   Size:         76     450     456      18     306      66     485

  Fragments arranged by size: 

                 485     456     450     306      76      66      18
 
BseYI C'CCAG_C

Cuts at:      0     126     810    1249    1857 
   Size:        126     684     439     608

  Fragments arranged by size: 

                 684     608     439     126
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1249    1309    1857 
   Size:       1249      60     548

  Fragments arranged by size: 

                1249     548      60
 
BsiEI CG_ry'CG

Cuts at:      0     313     919    1857 
   Size:        313     606     938

  Fragments arranged by size: 

                 938     606     313
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     155     248     974    1376    1857 
   Size:         68      87      93     726     402     481

  Fragments arranged by size: 

                 726     481     402      93      87      68
 
BsmI GAATG_Cn'

Cuts at:      0     665    1531    1857 
   Size:        665     866     326

  Fragments arranged by size: 

                 866     665     326
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     350     837    1857 
   Size:        350     487    1020

  Fragments arranged by size: 

                1020     487     350
 
Bsp1286I G_dGCh'C

Cuts at:      0      68      98     155     248     589     679     737     952 
   Size:         68      30      57      93     341      90      58     215

Cuts at:    952     974    1123    1376    1681    1820    1857 
   Size:         22     149     253     305     139      37

  Fragments arranged by size: 

                 341     305     253     215     149     139      93      90
                  68      58      57      37      30      22
 
BspHI T'CATG_A

Cuts at:      0    1712    1857 
   Size:       1712     145
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      29     299     432    1857 
   Size:         29     270     133    1425

  Fragments arranged by size: 

                1425     270     133      29
 
BsrBI CCG'CTC

Cuts at:      0     477    1487    1506    1857 
   Size:        477    1010      19     351

  Fragments arranged by size: 

                1010     477     351      19
 
BsrDI GCAATG_nn'

Cuts at:      0     209     455    1314    1857 
   Size:        209     246     859     543

  Fragments arranged by size: 

                 859     543     246     209
 
BsrFI r'CCGG_y

Cuts at:      0     214     237     308    1412    1857 
   Size:        214      23      71    1104     445

  Fragments arranged by size: 

                1104     445     214      71      23
 
BssSI C'ACGA_G

Cuts at:      0     148     472     933    1857 
   Size:        148     324     461     924

  Fragments arranged by size: 

                 924     461     324     148
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     159     302     662    1167    1459    1776    1857 
   Size:        159     143     360     505     292     317      81

  Fragments arranged by size: 

                 505     360     317     292     159     143      81
 
BstEII G'GTnAC_C

Cuts at:      0    1578    1857 
   Size:       1578     279
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1455    1809    1857 
   Size:       1455     354      48

  Fragments arranged by size: 

                1455     354      48
 
BstYI r'GATC_y

Cuts at:      0    1295    1727    1857 
   Size:       1295     432     130

  Fragments arranged by size: 

                1295     432     130
 
Bsu36I CC'TnA_GG

Cuts at:      0     786    1857 
   Size:        786    1071
 
BtgI C'CryG_G

Cuts at:      0      11     362     571     913     926    1337    1763    1857 
   Size:         11     351     209     342      13     411     426      94

  Fragments arranged by size: 

                 426     411     351     342     209      94      13      11
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1574    1857 
   Size:       1574     283
 
BtsI GCAGTG_nn'

Cuts at:      0     777    1478    1857 
   Size:        777     701     379

  Fragments arranged by size: 

                 777     701     379
 
DraIII CAC_nnn'GTG

Cuts at:      0     159    1332    1459    1586    1857 
   Size:        159    1173     127     127     271

  Fragments arranged by size: 

                1173     271     159     127     127
 
EaeI y'GGCC_r

Cuts at:      0     310     916    1097    1313    1501    1605    1799    1857 
   Size:        310     606     181     216     188     104     194      58

  Fragments arranged by size: 

                 606     310     216     194     188     181     104      58
            
 
EagI C'GGCC_G

Cuts at:      0     310     916    1857 
   Size:        310     606     941

  Fragments arranged by size: 

                 941     606     310
 
EarI CTCTTCn'nnn_

Cuts at:      0     103     994    1136    1857 
   Size:        103     891     142     721

  Fragments arranged by size: 

                 891     721     142     103
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     103     742    1857 
   Size:        103     639    1115

  Fragments arranged by size: 

                1115     639     103
 
EcoICRI GAG'CTC

Cuts at:      0     246     972    1857 
   Size:        246     726     885

  Fragments arranged by size: 

                 885     726     246
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     418     433     736     742     769    1015    1132 
   Size:         91     327      15     303       6      27     246     117

Cuts at:   1132    1375    1533    1587    1857 
   Size:        243     158      54     270

  Fragments arranged by size: 

                 327     303     270     246     243     158     117      91
                  54      27      15       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     415     751    1857 
   Size:        415     336    1106

  Fragments arranged by size: 

                1106     415     336
 
EcoO109I rG'GnC_Cy

Cuts at:      0     408     435    1224    1816    1817    1857 
   Size:        408      27     789     592       1      40

  Fragments arranged by size: 

                 789     592     408      40      27       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1005    1857 
   Size:       1005     852
 
FspI TGC'GCA

Cuts at:      0     295    1078    1857 
   Size:        295     783     779

  Fragments arranged by size: 

                 783     779     295
 
HaeII r_GCGC'y

Cuts at:      0     189    1857 
   Size:        189    1668
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     822     854    1582    1614    1857 
   Size:        822      32     728      32     243

  Fragments arranged by size: 

                 822     728     243      32      32
 
HincII GTy'rAC

Cuts at:      0     830    1410    1857 
   Size:        830     580     447

  Fragments arranged by size: 

                 830     580     447
 
HpaI GTT'AAC

Cuts at:      0    1410    1857 
   Size:       1410     447
 
KpnI G_GTAC'C

Cuts at:      0     492    1857 
   Size:        492    1365
 
MluI A'CGCG_T

Cuts at:      0     505    1857 
   Size:        505    1352
 
MscI TGG'CCA

Cuts at:      0    1099    1801    1857 
   Size:       1099     702      56

  Fragments arranged by size: 

                1099     702      56
 
MslI CAynn'nnrTG

Cuts at:      0     937    1105    1243    1342    1524    1686    1857 
   Size:        937     168     138      99     182     162     171

  Fragments arranged by size: 

                 937     182     171     168     162     138      99
 
MspA1I CmG'CkG

Cuts at:      0      13     573     702     915    1176    1263    1857 
   Size:         13     560     129     213     261      87     594

  Fragments arranged by size: 

                 594     560     261     213     129      87      13
 
NaeI GCC'GGC

Cuts at:      0     216    1857 
   Size:        216    1641
 
NcoI C'CATG_G

Cuts at:      0     362     926    1337    1857 
   Size:        362     564     411     520

  Fragments arranged by size: 

                 564     520     411     362
 
NgoMIV G'CCGG_C

Cuts at:      0     214    1857 
   Size:        214    1643
 
NsiI A_TGCA'T

Cuts at:      0    1531    1857 
   Size:       1531     326
 
NspI r_CATG'y

Cuts at:      0    1077    1779    1857 
   Size:       1077     702      78

  Fragments arranged by size: 

                1077     702      78
 
PspOMI G'GGCC_C

Cuts at:      0    1816    1857 
   Size:       1816      41
 
PspXI vC'TCGA_Gb

Cuts at:      0     322    1857 
   Size:        322    1535
 
PvuII CAG'CTG

Cuts at:      0     702    1176    1857 
   Size:        702     474     681

  Fragments arranged by size: 

                 702     681     474
 
SacI G_AGCT'C

Cuts at:      0     248     974    1857 
   Size:        248     726     883

  Fragments arranged by size: 

                 883     726     248
 
SacII CC_GC'GG

Cuts at:      0      14     574     916    1857 
   Size:         14     560     342     941

  Fragments arranged by size: 

                 941     560     342      14
 
SapI GCTCTTCn'nnn_

Cuts at:      0     103     994    1857 
   Size:        103     891     863

  Fragments arranged by size: 

                 891     863     103
 
ScaI AGT'ACT

Cuts at:      0     661    1857 
   Size:        661    1196
 
SexAI A'CCwGG_T

Cuts at:      0    1328    1857 
   Size:       1328     529
 
SfcI C'TryA_G

Cuts at:      0     954    1791    1857 
   Size:        954     837      66

  Fragments arranged by size: 

                 954     837      66
 
SmlI C'TyrA_G

Cuts at:      0     322     973    1507    1857 
   Size:        322     651     534     350

  Fragments arranged by size: 

                 651     534     350     322
 
SphI G_CATG'C

Cuts at:      0    1077    1779    1857 
   Size:       1077     702      78

  Fragments arranged by size: 

                1077     702      78
 
StuI AGG'CCT

Cuts at:      0     757    1594    1857 
   Size:        757     837     263

  Fragments arranged by size: 

                 837     757     263
 
StyI C'CwwG_G

Cuts at:      0     362     926    1337    1857 
   Size:        362     564     411     520

  Fragments arranged by size: 

                 564     520     411     362
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     562     833    1447    1857 
   Size:        562     271     614     410

  Fragments arranged by size: 

                 614     562     410     271
 
TatI w'GTAC_w

Cuts at:      0     659    1857 
   Size:        659    1198
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1318    1350    1857 
   Size:       1318      32     507

  Fragments arranged by size: 

                1318     507      32
 
XhoI C'TCGA_G

Cuts at:      0     322    1857 
   Size:        322    1535
 
XmnI GAAnn'nnTTC

Cuts at:      0     988    1857 
   Size:        988     869
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII     BbsI    BbvCI    BciVI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I   HincII
     HpaI     KpnI     MluI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NsiI     NspI   PspOMI    PspXI    PvuII     SacI    SacII
     SapI     ScaI    SexAI     SfcI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI     BclI    BglII     BmtI     BsaI    BsaBI    BsaWI    BsiWI
    BspEI    BsrGI   BssHII    BstBI  BstZ17I     ClaI    CspCI     DraI
     DrdI    EcoRI    EcoRV     FseI    FspAI  HindIII     KasI     MfeI
     MmeI     NarI     NdeI     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI     PsrI     PstI     PvuI    RsrII     SalI    SanDI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI  Tth111I     XbaI     XcmI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com