Restriction map for pF1KB0959
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwI        Aor51HI     ApaI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvrII       BalI        BamHI       
BanII       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaI        
BseAI       BsiEI       BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       Bst1107I    BstBI       
BstXI       BstYI       BstZ17I     CelII       ClaI        
CpoI        DpnII       DraI        DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MaeI        MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
NcoI        NdeI        NdeII       NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
ScaI        SexAI       SfiI        SfuI        SmaI        
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Alw44I        346  GTGCAC               G^TGCAC
AocI          261  CCTCAGG              CC^T[ACGT]AGG
ApaLI         346  GTGCAC               G^TGCAC
BbeI          225  GGCGCC               GGCGC^C
BbrPI         445  CACGTG               CAC^GTG
BcnI           23  CCGGG                CC^[GC]GG
BpmI          466  CTGGAG               CTGGAG(16/14)
BsaAI         445  CACGTG               [CT]AC^GT[GA]
BsaWI         335  ACCGGA               [AT]^CCGG[AT]
BseRI          99  CTCCTC               GAGGAG(10/8)
BsgI          232  GTGCAG               GTGCAG(16/14)
BsmBI         511  GAGACG               CGTCTC(1/5)
BspMI         299  ACCTGC               ACCTGC(4/8)
BssHII        328  GCGCGC               G^CGCGC
BssSI          38  CACGAG               CACGAG(-5/-1)
BstAPI        426  GCAGCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        354  GGTGACC              G^GT[ACGT]ACC
BstF5I        548  GGATG                GGATG(2/0)
BstPI         354  GGTGACC              G^GT[ACGT]ACC
Bsu36I        261  CCTCAGG              CC^T[ACGT]AGG
DraIII        349  CACGGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          441  CGGCCA               [CT]^GGCC[GA]
EarI           82  CTCTTC               CTCTTC(1/4)
Eco81I        261  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       354  GGTGACC              G^GT[ACGT]ACC
FokI          548  GGATG                GGATG(9/13)
HaeII         225  GGCGCC               [GA]GCGC^[CT]
KasI          225  GGCGCC               G^GCGCC
Ksp632I        82  CTCTTC               CTCTTC(1/4)
MaeIII        355  GTGAC                ^GT[ACGT]AC
MboII          83  TCTTC                GAAGA(8/7)
NaeI          439  GCCGGC               GCC^GGC
NarI          225  GGCGCC               GG^CGCC
NciI           23  CCGGG                CC^[GC]GG
NgoMIV        439  GCCGGC               G^CCGGC
NlaIII        275  CATG                 CATG^
NspI          274  ACATGC               [GA]CATG^[CT]
PmaCI         445  CACGTG               CAC^GTG
PmlI          445  CACGTG               CAC^GTG
PvuII         556  CAGCTG               CAG^CTG
SbfI          456  CCTGCAGG             CCTGCA^GG
SfaNI         549  GATGC                GCATC(5/9)
SfoI          225  GGCGCC               GGC^GCC
SgrAI         227  CGCCGGTG             C[GA]^CCGG[CT]G
Sse8387I      456  CCTGCAGG             CCTGCA^GG
StuI          362  AGGCCT               AGG^CCT
Tsp45I        355  GTGAC                ^GT[GC]AC
TspRI         344  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcyI          225  GGCGCC               G[GA]^CG[CT]C
              513  GACGCC              
AfaI          297  GTAC                 GT^AC
              544  GTAC                
AspHI         339  GAGCAC               G[AT]GC[AT]^C
              346  GTGCAC              
AvaII          26  GGTCC                G^G[AT]CC
              267  GGTCC               
AviII         302  TGCGCA               TGC^GCA
              449  TGCGCA              
BanI           19  GGCACC               G^G[CT][GA]CC
              225  GGCGCC              
BglI          278  GCCGCAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              417  GCCTCGCAGGC         
BsaHI         225  GGCGCC               G[GA]^CG[CT]C
              513  GACGCC              
BsiHKAI       339  GAGCAC               G[AT]GC[AT]^C
              346  GTGCAC              
BsmAI         511  GAGAC                GTCTC(1/5)
              126  GTCTC               
BsrFI         228  GCCGGT               [GA]^CCGG[CT]
              439  GCCGGC              
BsrI          171  CCAGT                ACTGG(1/-1)
              294  CCAGT               
Cfr10I        228  GCCGGT               [GA]^CCGG[CT]
              439  GCCGGC              
DdeI          241  CTGAG                C^T[ACGT]AG
              262  CTCAG               
FspI          302  TGCGCA               TGC^GCA
              449  TGCGCA              
Hin1I         225  GGCGCC               G[GA]^CG[CT]C
              513  GACGCC              
HinfI          76  GACTC                G^A[ACGT]TC
              145  GAGTC               
HphI          354  GGTGA                GGTGA(8/7)
               31  TCACC               
MaeII          58  ACGT                 A^CGT
              446  ACGT                
PleI           76  GACTC                GAGTC(4/5)
              145  GAGTC               
PpuMI          25  GGGTCCT              [GA]G^G[AT]CC[CT]
              266  GGGTCCC             
PstI          457  CTGCAG               CTGCA^G
              499  CTGCAG              
RsaI          297  GTAC                 GT^AC
              544  GTAC                
SfcI          457  CTGCAG               C^T[GA][CT]AG
              499  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       BbvI        
BmyI        BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DraII       EcoO109I    EcoRII      
FauI        Fnu4HI      HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       ItaI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NlaIV       Sau96I      ScrFI       TseI        
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