Restriction map for pF1KB0959
 (Circular) (Six-base) MAPSORT of: pF1KB0959.seq  Check: 1309  from: 1  to: 3683



pF1KB0959  3683 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AccI GT'mk_AC

Cuts at:    721     721 
   Size:       3683
 
Acc65I G'GTAC_C

Cuts at:    699     699 
   Size:       3683
 
AclI AA'CG_TT

Cuts at:   3523    3523 
   Size:       3683
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1508    1940    2687    2955    1508 
   Size:        432     747     268    2236

  Fragments arranged by size: 

                2236     747     432     268
 
AflIII A'CryG_T

Cuts at:   1035    2139    3116    1035 
   Size:       1104     977    1602

  Fragments arranged by size: 

                1602    1104     977
 
AgeI A'CCGG_T

Cuts at:   2071    2071 
   Size:       3683
 
AlwNI CAG_nnn'CTG

Cuts at:    348     408     540     573     666     988    2555     348 
   Size:         60     132      33      93     322    1567    1476

  Fragments arranged by size: 

                1567    1476     322     132      93      60      33
 
ApaLI G'TGCA_C

Cuts at:    454    2453     454 
   Size:       1999    1684
 
ApoI r'AATT_y

Cuts at:    687    3036    3292     687 
   Size:       2349     256    1078

  Fragments arranged by size: 

                2349    1078     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3683
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3683
 
AvaI C'yCGr_G

Cuts at:    703     703 
   Size:       3683
 
BamHI G'GATC_C

Cuts at:    708     708 
   Size:       3683
 
BanI G'GyrC_C

Cuts at:    127     333     699    1113    1361    1396     127 
   Size:        206     366     414     248      35    2414

  Fragments arranged by size: 

                2414     414     366     248     206      35
 
BanII G_rGCy'C

Cuts at:    697    1727     697 
   Size:       1030    2653
 
BbeI G_GCGC'C

Cuts at:    337    1117    1365     337 
   Size:        780     248    2655

  Fragments arranged by size: 

                2655     780     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   1581    2342    3683    1581 
   Size:        761    1341    1581

  Fragments arranged by size: 

                1581    1341     761
 
BclI T'GATC_A

Cuts at:   1203    1203 
   Size:       3683
 
BglI GCCn_nnn'nGGC

Cuts at:    392     531     392 
   Size:        139    3544
 
BglII A'GATC_T

Cuts at:   1198    3235    1198 
   Size:       2037    1646
 
BlpI GC'TnA_GC

Cuts at:    795     795 
   Size:       3683
 
Bme1580I G_kGCm'C

Cuts at:    130     458     595    1308    1401    2457     130 
   Size:        328     137     713      93    1056    1356

  Fragments arranged by size: 

                1356    1056     713     328     137      93
 
BmgBI CAC'GTC

Cuts at:    167     167 
   Size:       3683
 
BmrI ACTGGGnnnn_n'

Cuts at:    895     976    1311    3455     895 
   Size:         81     335    2144    1123

  Fragments arranged by size: 

                2144    1123     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2050    2050 
   Size:       3683
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    595     595 
   Size:       3683
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    859    1738    2230    2528     859 
   Size:        879     492     298    2014

  Fragments arranged by size: 

                2014     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3387    3387 
   Size:       3683
 
BsaAI yAC'GTr

Cuts at:    555    1036     555 
   Size:        481    3202
 
BsaBI GATnn'nnATC

Cuts at:      6    1197    3234       6 
   Size:       1191    2037     455

  Fragments arranged by size: 

                2037    1191     455
 
BsaHI Gr'CG_yC

Cuts at:    334     622    1114    1362    2064    3560     334 
   Size:        288     492     248     702    1496     457

  Fragments arranged by size: 

                1496     702     492     457     288     248
 
BsaWI w'CCGG_w

Cuts at:    443     872    1393    2071    2345    2492    2903     443 
   Size:        429     521     678     274     147     411    1223

  Fragments arranged by size: 

                1223     678     521     429     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    268     298     268 
   Size:         30    3653
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    198     198 
   Size:       3683
 
BseYI C'CCAG_C

Cuts at:    516     588    1108    1866    2443    2939     516 
   Size:         72     520     758     577     496    1260

  Fragments arranged by size: 

                1260     758     577     520     496      72
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    361     361 
   Size:       3683
 
BsiEI CG_ry'CG

Cuts at:    104    1225    1271    2479     104 
   Size:       1121      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1121      46
 
BsiHKAI G_wGCw'C

Cuts at:    451     458     697    1475    1665    2457     451 
   Size:          7     239     778     190     792    1677

  Fragments arranged by size: 

                1677     792     778     239     190       7
 
BsmBI CGTCTCn'nnnn_

Cuts at:    613     613 
   Size:       3683
 
Bsp1286I G_dGCh'C

Cuts at:    130     451     458     595     697    1308    1401    1475    1665 
   Size:        321       7     137     102     611      93      74     190

Cuts at:   1665    1727    2457     130 
   Size:         62     730    1356

  Fragments arranged by size: 

                1356     730     611     321     190     137     102      93
                  74      62       7
 
BspEI T'CCGG_A

Cuts at:   2903    2903 
   Size:       3683
 
BspHI T'CATG_A

Cuts at:   2859    2859 
   Size:       3683
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    416     733     947    1249    1630     416 
   Size:        317     214     302     381    2469

  Fragments arranged by size: 

                2469     381     317     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1975    2029    3513    3678      50 
   Size:       1925      54    1484     165      55

  Fragments arranged by size: 

                1925    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1595    1595 
   Size:       3683
 
BsrFI r'CCGG_y

Cuts at:    336     547    1681    1862    2071     336 
   Size:        211    1134     181     209    1948

  Fragments arranged by size: 

                1948    1134     211     209     181
 
BssHII G'CGCG_C

Cuts at:    436     436 
   Size:       3683
 
BssSI C'ACGA_G

Cuts at:    146    1954    2312     146 
   Size:       1808     358    1517

  Fragments arranged by size: 

                1808    1517     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    540    1060     540 
   Size:        520    3163
 
BstBI TT'CG_AA

Cuts at:   2044    2044 
   Size:       3683
 
BstEII G'GTnAC_C

Cuts at:    462     462 
   Size:       3683
 
BstYI r'GATC_y

Cuts at:      1     708    1175    1198    1533    1779    2791    2877    2889 
   Size:        707     467      23     335     246    1012      86      12

Cuts at:   2889    3235       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     707     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    370     370 
   Size:       3683
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3683
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1633    1814    1977    1633 
   Size:        181     163    3339

  Fragments arranged by size: 

                3339     181     163
 
BtsI GCAGTG_nn'

Cuts at:    926     926 
   Size:       3683
 
DraI TTT'AAA

Cuts at:    682     682 
   Size:       3683
 
DraIII CAC_nnn'GTG

Cuts at:    462     462 
   Size:       3683
 
DrdI GACnn_nn'nnGTC

Cuts at:   1389    2247    1389 
   Size:        858    2825
 
EaeI y'GGCC_r

Cuts at:    549    1268    1442    1833    1860    3205     549 
   Size:        719     174     391      27    1345    1027

  Fragments arranged by size: 

                1345    1027     719     391     174      27
 
EagI C'GGCC_G

Cuts at:   1268    1268 
   Size:       3683
 
EarI CTCTTCn'nnn_

Cuts at:    196    1706    1916     196 
   Size:       1510     210    1963

  Fragments arranged by size: 

                1963    1510     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1891    2201    2347    3493    1891 
   Size:        310     146    1146    2081

  Fragments arranged by size: 

                2081    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    695     695 
   Size:       3683
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    595    1508    1940    2687    2955     595 
   Size:        913     432     747     268    1323

  Fragments arranged by size: 

                1323     913     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    134     155     316     345     375     822     134 
   Size:         21     161      29      30     447    2995

  Fragments arranged by size: 

                2995     447     161      30      29      21
 
EcoRI G'AATT_C

Cuts at:    687     687 
   Size:       3683
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1702    1702 
   Size:       3683
 
FspI TGC'GCA

Cuts at:    412     559    1464     412 
   Size:        147     905    2631

  Fragments arranged by size: 

                2631     905     147
 
HaeII r_GCGC'y

Cuts at:    337    1117    1365    2387    3366     337 
   Size:        780     248    1022     979     654

  Fragments arranged by size: 

                1022     979     780     654     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1525    1555    1557    1587    1525 
   Size:         30       2      30    3621

  Fragments arranged by size: 

                3621      30      30       2
 
HincII GTy'rAC

Cuts at:    722     722 
   Size:       3683
 
KasI G'GCGC_C

Cuts at:    333    1113    1361     333 
   Size:        780     248    2655

  Fragments arranged by size: 

                2655     780     248
 
KpnI G_GTAC'C

Cuts at:    703     703 
   Size:       3683
 
MluI A'CGCG_T

Cuts at:   3116    3116 
   Size:       3683
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2354    2538    2354 
   Size:        184    3499
 
MscI TGG'CCA

Cuts at:   1444    1444 
   Size:       3683
 
MslI CAynn'nnrTG

Cuts at:   1672    1799    1672 
   Size:        127    3556
 
MspA1I CmG'CkG

Cuts at:    416     540     666     794    1108    1468    2481    2726     416 
   Size:        124     126     128     314     360    1013     245    1373

  Fragments arranged by size: 

                1373    1013     360     314     245     128     126     124
            
 
NaeI GCC'GGC

Cuts at:    549    1864     549 
   Size:       1315    2368
 
NarI GG'CG_CC

Cuts at:    334    1114    1362     334 
   Size:        780     248    2655

  Fragments arranged by size: 

                2655     780     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3683
 
NgoMIV G'CCGG_C

Cuts at:    547    1862     547 
   Size:       1315    2368
 
NspI r_CATG'y

Cuts at:    386     736    1236    2143     386 
   Size:        350     500     907    1926

  Fragments arranged by size: 

                1926     907     500     350
 
PciI A'CATG_T

Cuts at:   2139    2139 
   Size:       3683
 
PmeI GTTT'AAAC

Cuts at:    682     682 
   Size:       3683
 
PmlI CAC'GTG

Cuts at:    555     555 
   Size:       3683
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2822    2854    2822 
   Size:         32    3651
 
PpuMI rG'GwC_Cy

Cuts at:    134     375     134 
   Size:        241    3442
 
PstI C_TGCA'G

Cuts at:    569     611     730    1415     569 
   Size:         42     119     685    2837

  Fragments arranged by size: 

                2837     685     119      42
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3683
 
PvuII CAG'CTG

Cuts at:    666    1108    1468     666 
   Size:        442     360    2881

  Fragments arranged by size: 

                2881     442     360
 
RsrII CG'GwC_CG

Cuts at:   1878    1878 
   Size:       3683
 
SacI G_AGCT'C

Cuts at:    697     697 
   Size:       3683
 
SalI G'TCGA_C

Cuts at:    720     720 
   Size:       3683
 
SanDI GG'GwC_CC

Cuts at:    375     375 
   Size:       3683
 
SapI GCTCTTCn'nnn_

Cuts at:   1706    1916    1706 
   Size:        210    3473
 
SbfI CC_TGCA'GG

Cuts at:    569     730     569 
   Size:        161    3522
 
ScaI AGT'ACT

Cuts at:   3200    3200 
   Size:       3683
 
SfcI C'TryA_G

Cuts at:     33     565     607     726    1411    2404    2595      33 
   Size:        532      42     119     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     532     191     119      42
 
SfoI GGC'GCC

Cuts at:    335    1115    1363     335 
   Size:        780     248    2655

  Fragments arranged by size: 

                2655     780     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3683
 
SgrAI Cr'CCGG_yG

Cuts at:    336     336 
   Size:       3683
 
SmaI CCC'GGG

Cuts at:    705     705 
   Size:       3683
 
SmlI C'TyrA_G

Cuts at:    838    1753    2245    2507     838 
   Size:        915     492     262    2014

  Fragments arranged by size: 

                2014     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3683
 
SphI G_CATG'C

Cuts at:    736    1236     736 
   Size:        500    3183
 
StuI AGG'CCT

Cuts at:    472     472 
   Size:       3683
 
StyI C'CwwG_G

Cuts at:    107     817    1171     107 
   Size:        710     354    2619

  Fragments arranged by size: 

                2619     710     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1266    1399    1858    2067    1266 
   Size:        133     459     209    2882

  Fragments arranged by size: 

                2882     459     209     133
 
TatI w'GTAC_w

Cuts at:   3198    3198 
   Size:       3683
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    151     183    3200    3232     151 
   Size:         32    3017      32     602

  Fragments arranged by size: 

                3017     602      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1480    1480 
   Size:       3683
 
XbaI T'CTAG_A

Cuts at:    714     714 
   Size:       3683
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1178    1178 
   Size:       3683
 
XmaI C'CCGG_G

Cuts at:    703     703 
   Size:       3683
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PmlI     PpiI
    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BmtI   Bpu10I    BsiWI     BsmI    BsrGI    BstXI  BstZ17I     ClaI
    CspCI    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SexAI
     SfiI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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