(Circular) (Six-base) MAPSORT of: pF1KB0947.seq Check: 6410 from: 1 to: 3647 pF1KB0947 3647 bp With 182 enzymes: SgfI * October 7, 2009 14:15 .. AccI GT'mk_AC Cuts at: 394 685 394 Size: 291 3356 Acc65I G'GTAC_C Cuts at: 663 663 Size: 3647 AclI AA'CG_TT Cuts at: 3487 3487 Size: 3647 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 364 1472 1904 2651 2919 364 Size: 1108 432 747 268 1092 Fragments arranged by size: 1108 1092 747 432 268 AflIII A'CryG_T Cuts at: 999 2103 3080 999 Size: 1104 977 1566 Fragments arranged by size: 1566 1104 977 AgeI A'CCGG_T Cuts at: 2035 2035 Size: 3647 AlwNI CAG_nnn'CTG Cuts at: 634 952 2519 634 Size: 318 1567 1762 Fragments arranged by size: 1762 1567 318 ApaLI G'TGCA_C Cuts at: 2417 2417 Size: 3647 ApoI r'AATT_y Cuts at: 651 3000 3256 651 Size: 2349 256 1042 Fragments arranged by size: 2349 1042 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3647 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3647 AvaI C'yCGr_G Cuts at: 667 667 Size: 3647 BamHI G'GATC_C Cuts at: 135 672 135 Size: 537 3110 BanI G'GyrC_C Cuts at: 663 1077 1325 1360 663 Size: 414 248 35 2950 Fragments arranged by size: 2950 414 248 35 BanII G_rGCy'C Cuts at: 661 1691 661 Size: 1030 2617 BbeI G_GCGC'C Cuts at: 1081 1329 1081 Size: 248 3399 BbsI GAAGACnn'nnnn_ Cuts at: 258 258 Size: 3647 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 3613 BciVI GTATCCnnnnn_n' Cuts at: 1545 2306 3647 1545 Size: 761 1341 1545 Fragments arranged by size: 1545 1341 761 BclI T'GATC_A Cuts at: 1167 1167 Size: 3647 BglI GCCn_nnn'nGGC Cuts at: 112 112 Size: 3647 BglII A'GATC_T Cuts at: 1162 3199 1162 Size: 2037 1610 BlpI GC'TnA_GC Cuts at: 759 759 Size: 3647 Bme1580I G_kGCm'C Cuts at: 1272 1365 2421 1272 Size: 93 1056 2498 Fragments arranged by size: 2498 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 859 940 1275 3419 859 Size: 81 335 2144 1087 Fragments arranged by size: 2144 1087 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2014 2014 Size: 3647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 446 446 Size: 3647 Bpu10I CC'TnA_GC Cuts at: 514 514 Size: 3647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 823 1702 2194 2492 823 Size: 879 492 298 1978 Fragments arranged by size: 1978 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3351 3351 Size: 3647 BsaAI yAC'GTr Cuts at: 1000 1000 Size: 3647 BsaBI GATnn'nnATC Cuts at: 6 1161 3198 6 Size: 1155 2037 455 Fragments arranged by size: 2037 1155 455 BsaHI Gr'CG_yC Cuts at: 1078 1326 2028 3524 1078 Size: 248 702 1496 1201 Fragments arranged by size: 1496 1201 702 248 BsaWI w'CCGG_w Cuts at: 836 1357 2035 2309 2456 2867 836 Size: 521 678 274 147 411 1616 Fragments arranged by size: 1616 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 399 574 1072 1830 2407 2903 399 Size: 175 498 758 577 496 1143 Fragments arranged by size: 1143 758 577 498 496 175 BsiEI CG_ry'CG Cuts at: 104 1189 1235 2443 104 Size: 1085 46 1208 1308 Fragments arranged by size: 1308 1208 1085 46 BsiHKAI G_wGCw'C Cuts at: 661 1439 1629 2421 661 Size: 778 190 792 1887 Fragments arranged by size: 1887 792 778 190 Bsp1286I G_dGCh'C Cuts at: 661 1272 1365 1439 1629 1691 2421 661 Size: 611 93 74 190 62 730 1887 Fragments arranged by size: 1887 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 2867 2867 Size: 3647 BspHI T'CATG_A Cuts at: 611 2823 611 Size: 2212 1435 BspMI ACCTGCnnnn'nnnn_ Cuts at: 207 474 697 911 1213 1594 207 Size: 267 223 214 302 381 2260 Fragments arranged by size: 2260 381 302 267 223 214 BsrBI CCG'CTC Cuts at: 50 1939 1993 3477 3642 50 Size: 1889 54 1484 165 55 Fragments arranged by size: 1889 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1559 1559 Size: 3647 BsrFI r'CCGG_y Cuts at: 111 1645 1826 2035 111 Size: 1534 181 209 1723 Fragments arranged by size: 1723 1534 209 181 BssSI C'ACGA_G Cuts at: 1918 2276 1918 Size: 358 3289 BstAPI GCAn_nnn'nTGC Cuts at: 457 1024 457 Size: 567 3080 BstBI TT'CG_AA Cuts at: 2008 2008 Size: 3647 BstYI r'GATC_y Cuts at: 1 135 672 1139 1162 1497 1743 2755 2841 Size: 134 537 467 23 335 246 1012 86 Cuts at: 2841 2853 3199 1 Size: 12 346 449 Fragments arranged by size: 1012 537 467 449 346 335 246 134 86 23 12 BstZ17I GTA'TAC Cuts at: 395 395 Size: 3647 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1597 1778 1941 1597 Size: 181 163 3303 Fragments arranged by size: 3303 181 163 BtsI GCAGTG_nn' Cuts at: 890 890 Size: 3647 DraI TTT'AAA Cuts at: 646 646 Size: 3647 DrdI GACnn_nn'nnGTC Cuts at: 1353 2211 1353 Size: 858 2789 EaeI y'GGCC_r Cuts at: 1232 1406 1797 1824 3169 1232 Size: 174 391 27 1345 1710 Fragments arranged by size: 1710 1345 391 174 27 EagI C'GGCC_G Cuts at: 1232 1232 Size: 3647 EarI CTCTTCn'nnn_ Cuts at: 129 595 1670 1880 129 Size: 466 1075 210 1896 Fragments arranged by size: 1896 1075 466 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1855 2165 2311 3457 1855 Size: 310 146 1146 2045 Fragments arranged by size: 2045 1146 310 146 EcoICRI GAG'CTC Cuts at: 659 659 Size: 3647 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 364 446 1472 1904 2651 2919 364 Size: 82 1026 432 747 268 1092 Fragments arranged by size: 1092 1026 747 432 268 82 EcoO109I rG'GnC_Cy Cuts at: 196 305 631 786 196 Size: 109 326 155 3057 Fragments arranged by size: 3057 326 155 109 EcoRI G'AATT_C Cuts at: 651 651 Size: 3647 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1666 1666 Size: 3647 FspI TGC'GCA Cuts at: 1428 1428 Size: 3647 HaeII r_GCGC'y Cuts at: 1081 1329 2351 3330 1081 Size: 248 1022 979 1398 Fragments arranged by size: 1398 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1489 1519 1521 1551 1489 Size: 30 2 30 3585 Fragments arranged by size: 3585 30 30 2 HincII GTy'rAC Cuts at: 686 686 Size: 3647 KasI G'GCGC_C Cuts at: 1077 1325 1077 Size: 248 3399 KpnI G_GTAC'C Cuts at: 667 667 Size: 3647 MluI A'CGCG_T Cuts at: 3080 3080 Size: 3647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2318 2502 2318 Size: 184 3463 MscI TGG'CCA Cuts at: 1408 1408 Size: 3647 MslI CAynn'nnrTG Cuts at: 1636 1763 1636 Size: 127 3520 MspA1I CmG'CkG Cuts at: 758 1072 1432 2445 2690 758 Size: 314 360 1013 245 1715 Fragments arranged by size: 1715 1013 360 314 245 NaeI GCC'GGC Cuts at: 113 1828 113 Size: 1715 1932 NarI GG'CG_CC Cuts at: 1078 1326 1078 Size: 248 3399 NgoMIV G'CCGG_C Cuts at: 111 1826 111 Size: 1715 1932 NspI r_CATG'y Cuts at: 700 1200 2107 700 Size: 500 907 2240 Fragments arranged by size: 2240 907 500 PciI A'CATG_T Cuts at: 2103 2103 Size: 3647 PflMI CCAn_nnn'nTGG Cuts at: 581 581 Size: 3647 PfoI T'CCnGG_A Cuts at: 423 423 Size: 3647 PmeI GTTT'AAAC Cuts at: 646 646 Size: 3647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2786 2818 2786 Size: 32 3615 PpuMI rG'GwC_Cy Cuts at: 196 305 631 196 Size: 109 326 3212 Fragments arranged by size: 3212 326 109 PstI C_TGCA'G Cuts at: 694 1379 694 Size: 685 2962 PvuI CG_AT'CG Cuts at: 104 104 Size: 3647 PvuII CAG'CTG Cuts at: 1072 1432 1072 Size: 360 3287 RsrII CG'GwC_CG Cuts at: 1842 1842 Size: 3647 SacI G_AGCT'C Cuts at: 661 661 Size: 3647 SalI G'TCGA_C Cuts at: 684 684 Size: 3647 SapI GCTCTTCn'nnn_ Cuts at: 1670 1880 1670 Size: 210 3437 SbfI CC_TGCA'GG Cuts at: 694 694 Size: 3647 ScaI AGT'ACT Cuts at: 3164 3164 Size: 3647 SfcI C'TryA_G Cuts at: 33 690 1375 2368 2559 33 Size: 657 685 993 191 1121 Fragments arranged by size: 1121 993 685 657 191 SfoI GGC'GCC Cuts at: 1079 1327 1079 Size: 248 3399 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3647 SmaI CCC'GGG Cuts at: 669 669 Size: 3647 SmlI C'TyrA_G Cuts at: 802 1717 2209 2471 802 Size: 915 492 262 1978 Fragments arranged by size: 1978 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3647 SphI G_CATG'C Cuts at: 700 1200 700 Size: 500 3147 StyI C'CwwG_G Cuts at: 375 781 1135 375 Size: 406 354 2887 Fragments arranged by size: 2887 406 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1230 1363 1822 2031 1230 Size: 133 459 209 2846 Fragments arranged by size: 2846 459 209 133 TatI w'GTAC_w Cuts at: 3162 3162 Size: 3647 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 399 431 3164 3196 399 Size: 32 2733 32 850 Fragments arranged by size: 2733 850 32 32 Tth111I GACn'n_nGTC Cuts at: 1444 1444 Size: 3647 XbaI T'CTAG_A Cuts at: 678 678 Size: 3647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1142 1142 Size: 3647 XmaI C'CCGG_G Cuts at: 667 667 Size: 3647 XmnI GAAnn'nnTTC Cuts at: 550 550 Size: 3647 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI