Restriction map for pF1KE0632
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AflIII      
AgeI        AhdI        AlwI        AocI        Aor51HI     
AseI        AsnI        Asp700I     AspEI       AviII       
AvrII       BalI        BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaBI       BsaWI       
BseAI       BsiWI       BsmI        Bsp1407I    BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CpoI        
DraI        DraIII      Eam1105I    EarI        Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        Ksp632I     MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       Van91I      
XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         898  GACGTC               GACGT^C
Acc65I        594  GGTACC               G^GTACC
AcsI           82  GAATTC               [GA]^AATT[CT]
AfaI          595  GTAC                 GT^AC
ApaI         1111  GGGCCC               GGGCC^C
ApoI           82  GAATTC               [GA]^AATT[CT]
AscI          949  GGCGCGCC             GG^CGCGCC
Asp718I       594  GGTACC               G^GTACC
BbeI          529  GGCGCC               GGCGC^C
BfaI          254  CTAG                 C^TAG
BlpI         1044  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1044  GCTCAGC              GC^T[ACGT]AGC
BsaI          206  GAGACC               GGTCTC(1/5)
BsiEI         777  CGGCCG               CG[GA][CT]^CG
BsmBI         460  GAGACG               CGTCTC(1/5)
BspDI         439  ATCGAT               AT^CGAT
BspMI         209  ACCTGC               ACCTGC(4/8)
BsrI          918  ACTGG                ACTGG(1/-1)
BstAPI        996  GCAGCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1044  GCTCAGC              GC^T[ACGT]AGC
ClaI          439  ATCGAT               AT^CGAT
DpnII         708  GATC                 ^GATC
DrdI          892  GACAACGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          777  CGGCCG               C^GGCCG
EclXI         777  CGGCCG               C^GGCCG
Eco52I        777  CGGCCG               C^GGCCG
EcoRI          82  GAATTC               G^AATTC
HinfI          35  GATTC                G^A[ACGT]TC
KasI          529  GGCGCC               G^GCGCC
KpnI          594  GGTACC               GGTAC^C
MaeI          254  CTAG                 C^TAG
MboI          708  GATC                 ^GATC
NarI          529  GGCGCC               GG^CGCC
NdeII         708  GATC                 ^GATC
NotI          776  GCGGCCGC             GC^GGCCGC
PshAI        1063  GACCCCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          595  GTAC                 GT^AC
Sau3AI        708  GATC                 ^GATC
SfaNI         575  GCATC                GCATC(5/9)
SfoI          529  GGCGCC               GGC^GCC
SgrAI         466  CACCGGCG             C[GA]^CCGG[CT]G
TfiI           35  GATTC                G^A[AT]TC
Tsp509I        83  AATT                 ^AATT
XcmI          434  CCATCATCGATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI          529  GGCGCC               G[GA]^CG[CT]C
              898  GACGTC              
AlwNI         997  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1036  CAGCGGCTG           
BanII         324  GGGCTC               G[GA]GC[CT]^C
             1111  GGGCCC              
BbsI          678  GAAGAC               GAAGAC(2/6)
              277  GTCTTC              
BpuAI         678  GAAGAC               GAAGAC(2/6)
              277  GTCTTC              
BsaHI         529  GGCGCC               G[GA]^CG[CT]C
              898  GACGTC              
BseRI         295  GAGGAG               GAGGAG(10/8)
             1102  GAGGAG              
BsrFI         467  ACCGGC               [GA]^CCGG[CT]
              485  ACCGGC              
BssHII        356  GCGCGC               G^CGCGC
              950  GCGCGC              
Cfr10I        467  ACCGGC               [GA]^CCGG[CT]
              485  ACCGGC              
DsaI          818  CCGCGG               C^C[GA][CT]GG
             1115  CCGCGG              
EaeI          671  TGGCCG               [CT]^GGCC[GA]
              777  CGGCCG              
Hin1I         529  GGCGCC               G[GA]^CG[CT]C
              898  GACGTC              
HphI          654  GGTGA                GGTGA(8/7)
              734  TCACC               
KspI          818  CCGCGG               CCGC^GG
             1115  CCGCGG              
NlaIII        591  CATG                 CATG^
              702  CATG                
PstI          853  CTGCAG               CTGCA^G
             1003  CTGCAG              
SacII         818  CCGCGG               CCGC^GG
             1115  CCGCGG              
SexAI         449  ACCTGGT              A^CC[AT]GGT
              689  ACCAGGT             
SfcI          853  CTGCAG               C^T[GA][CT]AG
             1003  CTGCAG              
TaqI          440  TCGA                 T^CGA
              908  TCGA                
TthHB8I       440  TCGA                 T^CGA
              908  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AluI        Alw44I      ApaLI       
AspHI       AspI        AvaI        AvaII       BanI        
BbvI        BcnI        BmyI        BpmI        BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DraII       EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HpaII       ItaI        
MaeII       MaeIII      MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NlaIV       PflFI       PpuMI       Sau96I      
ScrFI       SmaI        TseI        Tsp45I      TspRI       
Tth111I     XmaCI       XmaI        
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