Restriction map for pF1KE0632
 (Circular) (Six-base) MAPSORT of: pF1KE0632.seq  Check: 4019  from: 1  to: 4241



pF1KE0632  4241 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AatII G_ACGT'C

Cuts at:   1010    1010 
   Size:       4241
 
AccI GT'mk_AC

Cuts at:   1279    1279 
   Size:       4241
 
Acc65I G'GTAC_C

Cuts at:    702    1257     702 
   Size:        555    3686
 
AclI AA'CG_TT

Cuts at:   4081    4081 
   Size:       4241
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2066    2498    3245    3513    2066 
   Size:        432     747     268    2794

  Fragments arranged by size: 

                2794     747     432     268
 
AflIII A'CryG_T

Cuts at:   1593    2697    3674    1593 
   Size:       1104     977    2160

  Fragments arranged by size: 

                2160    1104     977
 
AgeI A'CCGG_T

Cuts at:   2629    2629 
   Size:       4241
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    748     748 
   Size:       4241
 
AlwNI CAG_nnn'CTG

Cuts at:   1110    1149    1546    3113    1110 
   Size:         39     397    1567    2238

  Fragments arranged by size: 

                2238    1567     397      39
 
ApaI G_GGCC'C

Cuts at:   1223    1223 
   Size:       4241
 
ApaLI G'TGCA_C

Cuts at:    256     307     494    3011     256 
   Size:         51     187    2517    1486

  Fragments arranged by size: 

                2517    1486     187      51
 
ApoI r'AATT_y

Cuts at:    190    1245    3594    3850     190 
   Size:       1055    2349     256     581

  Fragments arranged by size: 

                2349    1055     581     256
 
AscI GG'CGCG_CC

Cuts at:   1058    1058 
   Size:       4241
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4241
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4241
 
AvaI C'yCGr_G

Cuts at:    565     706     903    1053    1079    1261     565 
   Size:        141     197     150      26     182    3545

  Fragments arranged by size: 

                3545     197     182     150     141      26
 
BamHI G'GATC_C

Cuts at:   1266    1266 
   Size:       4241
 
BanI G'GyrC_C

Cuts at:    637     675     702     922    1257    1671    1919    1954     637 
   Size:         38      27     220     335     414     248      35    2924

  Fragments arranged by size: 

                2924     414     335     248     220      38      35      27
            
 
BanII G_rGCy'C

Cuts at:    436    1223    1255    2285     436 
   Size:        787      32    1030    2392

  Fragments arranged by size: 

                2392    1030     787      32
 
BbeI G_GCGC'C

Cuts at:    641    1675    1923     641 
   Size:       1034     248    2959

  Fragments arranged by size: 

                2959    1034     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    378     793     378 
   Size:        415    3826
 
BciVI GTATCCnnnnn_n'

Cuts at:   2139    2900    4241    2139 
   Size:        761    1341    2139

  Fragments arranged by size: 

                2139    1341     761
 
BclI T'GATC_A

Cuts at:   1761    1761 
   Size:       4241
 
BglII A'GATC_T

Cuts at:   1756    3793    1756 
   Size:       2037    2204
 
BlpI GC'TnA_GC

Cuts at:   1153    1353    1153 
   Size:        200    4041
 
Bme1580I G_kGCm'C

Cuts at:    260     311     498     680    1024    1223    1866    1959    3015 
   Size:         51     187     182     344     199     643      93    1056

Cuts at:   3015     260 
   Size:       1486

  Fragments arranged by size: 

                1486    1056     643     344     199     187     182      93
                  51
 
BmgBI CAC'GTC

Cuts at:   1039    1039 
   Size:       4241
 
BmrI ACTGGGnnnn_n'

Cuts at:   1453    1534    1869    4013    1453 
   Size:         81     335    2144    1681

  Fragments arranged by size: 

                2144    1681     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2608    2608 
   Size:       4241
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    688     778    1048    1126     688 
   Size:         90     270      78    3803

  Fragments arranged by size: 

                3803     270      90      78
 
Bpu10I CC'TnA_GC

Cuts at:    379     379 
   Size:       4241
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1417    2296    2788    3086    1417 
   Size:        879     492     298    2572

  Fragments arranged by size: 

                2572     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    308    3945     308 
   Size:       3637     604
 
BsaAI yAC'GTr

Cuts at:   1594    1594 
   Size:       4241
 
BsaBI GATnn'nnATC

Cuts at:      6    1755    3792       6 
   Size:       1749    2037     455

  Fragments arranged by size: 

                2037    1749     455
 
BsaHI Gr'CG_yC

Cuts at:    638    1007    1672    1920    2622    4118     638 
   Size:        369     665     248     702    1496     761

  Fragments arranged by size: 

                1496     761     702     665     369     248
 
BsaWI w'CCGG_w

Cuts at:   1430    1951    2629    2903    3050    3461    1430 
   Size:        521     678     274     147     411    2210

  Fragments arranged by size: 

                2210     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    256     286     256 
   Size:         30    4211
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    418    1225     418 
   Size:        807    3434
 
BseYI C'CCAG_C

Cuts at:    459     615    1666    2424    3001    3497     459 
   Size:        156    1051     758     577     496    1203

  Fragments arranged by size: 

                1203    1051     758     577     496     156
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    462     574    1019     462 
   Size:        112     445    3684

  Fragments arranged by size: 

                3684     445     112
 
BsiEI CG_ry'CG

Cuts at:    104     888    1783    1829    3037     104 
   Size:        784     895      46    1208    1308

  Fragments arranged by size: 

                1308    1208     895     784      46
 
BsiHKAI G_wGCw'C

Cuts at:    260     311     498     727    1217    1255    2033    2223    3015 
   Size:         51     187     229     490      38     778     190     792

Cuts at:   3015     260 
   Size:       1486

  Fragments arranged by size: 

                1486     792     778     490     229     190     187      51
                  38
 
BsmBI CGTCTCn'nnnn_

Cuts at:    562     562 
   Size:       4241
 
Bsp1286I G_dGCh'C

Cuts at:    260     311     436     498     680     727    1024    1217    1223 
   Size:         51     125      62     182      47     297     193       6

Cuts at:   1223    1255    1866    1959    2033    2223    2285    3015     260 
   Size:         32     611      93      74     190      62     730    1486

  Fragments arranged by size: 

                1486     730     611     297     193     190     182     125
                  93      74      62      62      51      47      32       6
            
 
BspEI T'CCGG_A

Cuts at:   3461    3461 
   Size:       4241
 
BspHI T'CATG_A

Cuts at:   3417    3417 
   Size:       4241
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    326    1291    1505    1807    2188     326 
   Size:        965     214     302     381    2379

  Fragments arranged by size: 

                2379     965     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2533    2587    4071    4236      50 
   Size:       2483      54    1484     165      55

  Fragments arranged by size: 

                2483    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2153    2153 
   Size:       4241
 
BsrFI r'CCGG_y

Cuts at:    575     593    2239    2420    2629     575 
   Size:         18    1646     181     209    2187

  Fragments arranged by size: 

                2187    1646     209     181      18
 
BssHII G'CGCG_C

Cuts at:    464    1058     464 
   Size:        594    3647
 
BssSI C'ACGA_G

Cuts at:   2512    2870    2512 
   Size:        358    3883
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1110    1618    1110 
   Size:        508    3733
 
BstBI TT'CG_AA

Cuts at:   2602    2602 
   Size:       4241
 
BstYI r'GATC_y

Cuts at:      1    1266    1733    1756    2091    2337    3349    3435    3447 
   Size:       1265     467      23     335     246    1012      86      12

Cuts at:   3447    3793       1 
   Size:        346     449

  Fragments arranged by size: 

                1265    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    926    1223     926 
   Size:        297    3944
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    647    1007    2191    2372    2535     647 
   Size:        360    1184     181     163    2353

  Fragments arranged by size: 

                2353    1184     360     181     163
 
BtsI GCAGTG_nn'

Cuts at:    167    1484     167 
   Size:       1317    2924
 
ClaI AT'CG_AT

Cuts at:    548     548 
   Size:       4241
 
DraI TTT'AAA

Cuts at:   1240    1240 
   Size:       4241
 
DrdI GACnn_nn'nnGTC

Cuts at:   1006    1947    2805    1006 
   Size:        941     858    2442

  Fragments arranged by size: 

                2442     941     858
 
EaeI y'GGCC_r

Cuts at:    779     885    1826    2000    2391    2418    3763     779 
   Size:        106     941     174     391      27    1345    1257

  Fragments arranged by size: 

                1345    1257     941     391     174     106      27
 
EagI C'GGCC_G

Cuts at:    885    1826     885 
   Size:        941    3300
 
EarI CTCTTCn'nnn_

Cuts at:   2264    2474    2264 
   Size:        210    4031
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    202     462     517     733    1000    2449    2759    2905    4051 
   Size:        260      55     216     267    1449     310     146    1146

Cuts at:   4051     202 
   Size:        392

  Fragments arranged by size: 

                1449    1146     392     310     267     260     216     146
                  55
 
EcoICRI GAG'CTC

Cuts at:   1253    1253 
   Size:       4241
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    688     778    1048    1126    2066    2498    3245    3513     688 
   Size:         90     270      78     940     432     747     268    1416

  Fragments arranged by size: 

                1416     940     747     432     270     268      90      78
            
 
EcoO109I rG'GnC_Cy

Cuts at:    481     711     753     940    1119    1380     481 
   Size:        230      42     187     179     261    3342

  Fragments arranged by size: 

                3342     261     230     187     179      42
 
EcoRI G'AATT_C

Cuts at:    190    1245     190 
   Size:       1055    3186
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2260    2260 
   Size:       4241
 
FspI TGC'GCA

Cuts at:   2022    2022 
   Size:       4241
 
HaeII r_GCGC'y

Cuts at:    460     641    1192    1675    1923    2945    3924     460 
   Size:        181     551     483     248    1022     979     777

  Fragments arranged by size: 

                1022     979     777     551     483     248     181
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2083    2113    2115    2145    2083 
   Size:         30       2      30    4179

  Fragments arranged by size: 

                4179      30      30       2
 
HincII GTy'rAC

Cuts at:   1280    1280 
   Size:       4241
 
KasI G'GCGC_C

Cuts at:    637    1671    1919     637 
   Size:       1034     248    2959

  Fragments arranged by size: 

                2959    1034     248
 
KpnI G_GTAC'C

Cuts at:    706    1261     706 
   Size:        555    3686
 
MluI A'CGCG_T

Cuts at:   3674    3674 
   Size:       4241
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    299    2912    3096     299 
   Size:       2613     184    1444

  Fragments arranged by size: 

                2613    1444     184
 
MscI TGG'CCA

Cuts at:   2002    2002 
   Size:       4241
 
MslI CAynn'nnrTG

Cuts at:    550     775     808    2230    2357     550 
   Size:        225      33    1422     127    2434

  Fragments arranged by size: 

                2434    1422     225     127      33
 
MspA1I CmG'CkG

Cuts at:    615     928    1107    1116    1146    1225    1352    1666    2026 
   Size:        313     179       9      30      79     127     314     360

Cuts at:   2026    3039    3284     615 
   Size:       1013     245    1572

  Fragments arranged by size: 

                1572    1013     360     314     313     245     179     127
                  79      30       9
 
NaeI GCC'GGC

Cuts at:   2422    2422 
   Size:       4241
 
NarI GG'CG_CC

Cuts at:    638    1672    1920     638 
   Size:       1034     248    2959

  Fragments arranged by size: 

                2959    1034     248
 
NgoMIV G'CCGG_C

Cuts at:   2420    2420 
   Size:       4241
 
NotI GC'GGCC_GC

Cuts at:    885     885 
   Size:       4241
 
NspI r_CATG'y

Cuts at:   1294    1794    2701    1294 
   Size:        500     907    2834

  Fragments arranged by size: 

                2834     907     500
 
PciI A'CATG_T

Cuts at:   2697    2697 
   Size:       4241
 
PfoI T'CCnGG_A

Cuts at:    755     755 
   Size:       4241
 
PmeI GTTT'AAAC

Cuts at:   1240    1240 
   Size:       4241
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3380    3412    3380 
   Size:         32    4209
 
PpuMI rG'GwC_Cy

Cuts at:    481     753     940    1119     481 
   Size:        272     187     179    3603

  Fragments arranged by size: 

                3603     272     187     179
 
PshAI GACnn'nnGTC

Cuts at:   1175    1175 
   Size:       4241
 
PspOMI G'GGCC_C

Cuts at:   1219    1219 
   Size:       4241
 
PstI C_TGCA'G

Cuts at:    965    1115    1288    1973     965 
   Size:        150     173     685    3233

  Fragments arranged by size: 

                3233     685     173     150
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4241
 
PvuII CAG'CTG

Cuts at:   1666    2026    1666 
   Size:        360    3881
 
RsrII CG'GwC_CG

Cuts at:   2436    2436 
   Size:       4241
 
SacI G_AGCT'C

Cuts at:   1255    1255 
   Size:       4241
 
SacII CC_GC'GG

Cuts at:    929    1226     929 
   Size:        297    3944
 
SalI G'TCGA_C

Cuts at:   1278    1278 
   Size:       4241
 
SanDI GG'GwC_CC

Cuts at:    940     940 
   Size:       4241
 
SapI GCTCTTCn'nnn_

Cuts at:   2264    2474    2264 
   Size:        210    4031
 
SbfI CC_TGCA'GG

Cuts at:   1288    1288 
   Size:       4241
 
ScaI AGT'ACT

Cuts at:   3758    3758 
   Size:       4241
 
SexAI A'CCwGG_T

Cuts at:    557     797     557 
   Size:        240    4001
 
SfcI C'TryA_G

Cuts at:     33     961    1111    1284    1969    2962    3153      33 
   Size:        928     150     173     685     993     191    1121

  Fragments arranged by size: 

                1121     993     928     685     191     173     150
 
SfoI GGC'GCC

Cuts at:    639    1673    1921     639 
   Size:       1034     248    2959

  Fragments arranged by size: 

                2959    1034     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4241
 
SgrAI Cr'CCGG_yG

Cuts at:    575     575 
   Size:       4241
 
SmaI CCC'GGG

Cuts at:    708    1055    1081    1263     708 
   Size:        347      26     182    3686

  Fragments arranged by size: 

                3686     347     182      26
 
SmlI C'TyrA_G

Cuts at:   1396    2311    2803    3065    1396 
   Size:        915     492     262    2572

  Fragments arranged by size: 

                2572     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4241
 
SphI G_CATG'C

Cuts at:   1294    1794    1294 
   Size:        500    3741
 
StyI C'CwwG_G

Cuts at:   1375    1729    1375 
   Size:        354    3887
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    277    1824    1957    2416    2625     277 
   Size:       1547     133     459     209    1893

  Fragments arranged by size: 

                1893    1547     459     209     133
 
TatI w'GTAC_w

Cuts at:   3756    3756 
   Size:       4241
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    256     288    3758    3790     256 
   Size:         32    3470      32     707

  Fragments arranged by size: 

                3470     707      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    559     751     799    1009    2038     559 
   Size:        192      48     210    1029    2762

  Fragments arranged by size: 

                2762    1029     210     192      48
 
XbaI T'CTAG_A

Cuts at:   1272    1272 
   Size:       4241
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    549    1736     549 
   Size:       1187    3054
 
XmaI C'CCGG_G

Cuts at:    706    1053    1079    1261     706 
   Size:        347      26     182    3686

  Fragments arranged by size: 

                3686     347     182      26
 
ZraI GAC'GTC

Cuts at:   1008    1008 
   Size:       4241
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NotI     NspI     PciI
     PfoI     PmeI     PpiI    PpuMI    PshAI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AleI     AloI    AvrII     BaeI
    BbvCI     BcgI    BfrBI     BglI     BmtI    BsiWI     BsmI    BsrGI
   BstEII    BstXI  BstZ17I   Bsu36I    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI
     NruI     NsiI     PacI     PasI    PflMI     PmlI     PsiI    PspXI
     PsrI     SfiI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
     XmnI
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