Restriction map for pF1KB0945
 (Circular) (Six-base) MAPSORT of: pF1KB0945.seq  Check: 6076  from: 1  to: 3623



pF1KB0945  3623 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AccI GT'mk_AC

Cuts at:    317     506     661     317 
   Size:        189     155    3279

  Fragments arranged by size: 

                3279     189     155
 
Acc65I G'GTAC_C

Cuts at:    639     639 
   Size:       3623
 
AclI AA'CG_TT

Cuts at:   3463    3463 
   Size:       3623
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1448    1880    2627    2895    1448 
   Size:        432     747     268    2176

  Fragments arranged by size: 

                2176     747     432     268
 
AflIII A'CryG_T

Cuts at:    975    2079    3056     975 
   Size:       1104     977    1542

  Fragments arranged by size: 

                1542    1104     977
 
AgeI A'CCGG_T

Cuts at:   2011    2011 
   Size:       3623
 
AlwNI CAG_nnn'CTG

Cuts at:    928    2495     928 
   Size:       1567    2056
 
ApaLI G'TGCA_C

Cuts at:   2393    2393 
   Size:       3623
 
ApoI r'AATT_y

Cuts at:    328     562     627    2976    3232     328 
   Size:        234      65    2349     256     719

  Fragments arranged by size: 

                2349     719     256     234      65
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3623
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3623
 
AvaI C'yCGr_G

Cuts at:    643     643 
   Size:       3623
 
BamHI G'GATC_C

Cuts at:    648     648 
   Size:       3623
 
BanI G'GyrC_C

Cuts at:    639    1053    1301    1336     639 
   Size:        414     248      35    2926

  Fragments arranged by size: 

                2926     414     248      35
 
BanII G_rGCy'C

Cuts at:    637    1667     637 
   Size:       1030    2593
 
BbeI G_GCGC'C

Cuts at:   1057    1305    1057 
   Size:        248    3375
 
BciVI GTATCCnnnnn_n'

Cuts at:   1521    2282    3623    1521 
   Size:        761    1341    1521

  Fragments arranged by size: 

                1521    1341     761
 
BclI T'GATC_A

Cuts at:   1143    1143 
   Size:       3623
 
BglII A'GATC_T

Cuts at:   1138    3175    1138 
   Size:       2037    1586
 
BlpI GC'TnA_GC

Cuts at:    735     735 
   Size:       3623
 
Bme1580I G_kGCm'C

Cuts at:   1248    1341    2397    1248 
   Size:         93    1056    2474

  Fragments arranged by size: 

                2474    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    835     916    1251    3395     835 
   Size:         81     335    2144    1063

  Fragments arranged by size: 

                2144    1063     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    360    1990     360 
   Size:       1630    1993
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    799    1678    2170    2468     799 
   Size:        879     492     298    1954

  Fragments arranged by size: 

                1954     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3327    3327 
   Size:       3623
 
BsaAI yAC'GTr

Cuts at:    976     976 
   Size:       3623
 
BsaBI GATnn'nnATC

Cuts at:      6    1137    3174       6 
   Size:       1131    2037     455

  Fragments arranged by size: 

                2037    1131     455
 
BsaHI Gr'CG_yC

Cuts at:    341    1054    1302    2004    3500     341 
   Size:        713     248     702    1496     464

  Fragments arranged by size: 

                1496     713     702     464     248
 
BsaWI w'CCGG_w

Cuts at:    812    1333    2011    2285    2432    2843     812 
   Size:        521     678     274     147     411    1592

  Fragments arranged by size: 

                1592     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1048    1806    2383    2879    1048 
   Size:        758     577     496    1792

  Fragments arranged by size: 

                1792     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1165    1211    2419     104 
   Size:       1061      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1061      46
 
BsiHKAI G_wGCw'C

Cuts at:    145     637    1415    1605    2397     145 
   Size:        492     778     190     792    1371

  Fragments arranged by size: 

                1371     792     778     492     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    348     348 
   Size:       3623
 
Bsp1286I G_dGCh'C

Cuts at:    145     637    1248    1341    1415    1605    1667    2397     145 
   Size:        492     611      93      74     190      62     730    1371

  Fragments arranged by size: 

                1371     730     611     492     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2843    2843 
   Size:       3623
 
BspHI T'CATG_A

Cuts at:   2799    2799 
   Size:       3623
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    673     887    1189    1570     673 
   Size:        214     302     381    2726

  Fragments arranged by size: 

                2726     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1915    1969    3453    3618      50 
   Size:       1865      54    1484     165      55

  Fragments arranged by size: 

                1865    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1535    1535 
   Size:       3623
 
BsrFI r'CCGG_y

Cuts at:   1621    1802    2011    1621 
   Size:        181     209    3233

  Fragments arranged by size: 

                3233     209     181
 
BssSI C'ACGA_G

Cuts at:   1894    2252    1894 
   Size:        358    3265
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1000    1000 
   Size:       3623
 
BstBI TT'CG_AA

Cuts at:   1984    1984 
   Size:       3623
 
BstYI r'GATC_y

Cuts at:      1     648    1115    1138    1473    1719    2731    2817    2829 
   Size:        647     467      23     335     246    1012      86      12

Cuts at:   2829    3175       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     647     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3623
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1573    1754    1917    1573 
   Size:        181     163    3279

  Fragments arranged by size: 

                3279     181     163
 
BtsI GCAGTG_nn'

Cuts at:    866     866 
   Size:       3623
 
DraI TTT'AAA

Cuts at:    324     622     324 
   Size:        298    3325
 
DrdI GACnn_nn'nnGTC

Cuts at:   1329    2187    1329 
   Size:        858    2765
 
EaeI y'GGCC_r

Cuts at:   1208    1382    1773    1800    3145    1208 
   Size:        174     391      27    1345    1686

  Fragments arranged by size: 

                1686    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1208    1208 
   Size:       3623
 
EarI CTCTTCn'nnn_

Cuts at:    224     448    1646    1856     224 
   Size:        224    1198     210    1991

  Fragments arranged by size: 

                1991    1198     224     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1831    2141    2287    3433    1831 
   Size:        310     146    1146    2021

  Fragments arranged by size: 

                2021    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    635     635 
   Size:       3623
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1448    1880    2627    2895    1448 
   Size:        432     747     268    2176

  Fragments arranged by size: 

                2176     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    762     762 
   Size:       3623
 
EcoRI G'AATT_C

Cuts at:    328     562     627     328 
   Size:        234      65    3324

  Fragments arranged by size: 

                3324     234      65
 
EcoRV GAT'ATC

Cuts at:    591     591 
   Size:       3623
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    146    1642     146 
   Size:       1496    2127
 
FspI TGC'GCA

Cuts at:   1404    1404 
   Size:       3623
 
HaeII r_GCGC'y

Cuts at:   1057    1305    2327    3306    1057 
   Size:        248    1022     979    1374

  Fragments arranged by size: 

                1374    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    223     255    1465    1495    1497    1527     223 
   Size:         32    1210      30       2      30    2319

  Fragments arranged by size: 

                2319    1210      32      30      30       2
 
HincII GTy'rAC

Cuts at:    662     662 
   Size:       3623
 
HindIII A'AGCT_T

Cuts at:    193     193 
   Size:       3623
 
KasI G'GCGC_C

Cuts at:   1053    1301    1053 
   Size:        248    3375
 
KpnI G_GTAC'C

Cuts at:    643     643 
   Size:       3623
 
MluI A'CGCG_T

Cuts at:   3056    3056 
   Size:       3623
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2294    2478    2294 
   Size:        184    3439
 
MscI TGG'CCA

Cuts at:   1384    1384 
   Size:       3623
 
MslI CAynn'nnrTG

Cuts at:    152    1612    1739     152 
   Size:       1460     127    2036

  Fragments arranged by size: 

                2036    1460     127
 
MspA1I CmG'CkG

Cuts at:    734    1048    1408    2421    2666     734 
   Size:        314     360    1013     245    1691

  Fragments arranged by size: 

                1691    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1804    1804 
   Size:       3623
 
NarI GG'CG_CC

Cuts at:   1054    1302    1054 
   Size:        248    3375
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3623
 
NgoMIV G'CCGG_C

Cuts at:   1802    1802 
   Size:       3623
 
NspI r_CATG'y

Cuts at:    676    1176    2083     676 
   Size:        500     907    2216

  Fragments arranged by size: 

                2216     907     500
 
PciI A'CATG_T

Cuts at:   2079    2079 
   Size:       3623
 
PmeI GTTT'AAAC

Cuts at:    622     622 
   Size:       3623
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2762    2794    2762 
   Size:         32    3591
 
PstI C_TGCA'G

Cuts at:    670    1355     670 
   Size:        685    2938
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3623
 
PvuII CAG'CTG

Cuts at:   1048    1408    1048 
   Size:        360    3263
 
RsrII CG'GwC_CG

Cuts at:   1818    1818 
   Size:       3623
 
SacI G_AGCT'C

Cuts at:    637     637 
   Size:       3623
 
SalI G'TCGA_C

Cuts at:    660     660 
   Size:       3623
 
SapI GCTCTTCn'nnn_

Cuts at:   1646    1856    1646 
   Size:        210    3413
 
SbfI CC_TGCA'GG

Cuts at:    670     670 
   Size:       3623
 
ScaI AGT'ACT

Cuts at:   3140    3140 
   Size:       3623
 
SfcI C'TryA_G

Cuts at:     33     666    1351    2344    2535      33 
   Size:        633     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     633     191
 
SfoI GGC'GCC

Cuts at:   1055    1303    1055 
   Size:        248    3375
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3623
 
SmaI CCC'GGG

Cuts at:    645     645 
   Size:       3623
 
SmlI C'TyrA_G

Cuts at:    778    1693    2185    2447     778 
   Size:        915     492     262    1954

  Fragments arranged by size: 

                1954     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3623
 
SphI G_CATG'C

Cuts at:    676    1176     676 
   Size:        500    3123
 
StyI C'CwwG_G

Cuts at:    107     757    1111     107 
   Size:        650     354    2619

  Fragments arranged by size: 

                2619     650     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1206    1339    1798    2007    1206 
   Size:        133     459     209    2822

  Fragments arranged by size: 

                2822     459     209     133
 
TatI w'GTAC_w

Cuts at:    270    3138     270 
   Size:       2868     755
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3140    3172    3140 
   Size:         32    3591
 
Tth111I GACn'n_nGTC

Cuts at:   1420    1420 
   Size:       3623
 
XbaI T'CTAG_A

Cuts at:    654     654 
   Size:       3623
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1118    1118 
   Size:       3623
 
XmaI C'CCGG_G

Cuts at:    643     643 
   Size:       3623
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI
    BsiWI     BsmI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI     FseI    FspAI     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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